scholarly journals Mechanism of topology simplification by type II DNA topoisomerases

2001 ◽  
Vol 98 (6) ◽  
pp. 3045-3049 ◽  
Author(s):  
A. V. Vologodskii ◽  
W. Zhang ◽  
V. V. Rybenkov ◽  
A. A. Podtelezhnikov ◽  
D. Subramanian ◽  
...  
Keyword(s):  
Type Ii ◽  
Dna Topoisomerases ◽  
Type Ii Dna Topoisomerases ◽  
Topology Simplification

The role of ATP in the reactions of type II DNA topoisomerases

2010 ◽  
Vol 38 (2) ◽  
pp. 438-442 ◽  
Author(s):  
Andrew D. Bates ◽  
Anthony Maxwell
Keyword(s):  
Atp Hydrolysis ◽  
Dna Gyrase ◽  
Dna Topology ◽  
Type Ii ◽  
Free Energies ◽  
Dna Topoisomerases ◽  
Type Ii Dna Topoisomerases ◽  
Topology Simplification ◽  
Hydrolysis Of

Type II DNA topoisomerases catalyse changes in DNA topology in reactions coupled to the hydrolysis of ATP. In the case of DNA gyrase, which can introduce supercoils into DNA, the requirement for free energy is clear. However, the non-supercoiling type II enzymes carry out reactions that are apparently energetically favourable, so their requirement for ATP hydrolysis is not so obvious. It has been shown that many of these enzymes (the type IIA family) can simplify the topology of their DNA substrates to a level beyond that expected at equilibrium. Although this seems to explain their usage of ATP, we show that the free energies involved in topology simplification are very small (<0.2% of that available from ATP) and we argue that topology simplification may simply be an evolutionary relic.

scholarly journals DNA G-segment bending is not the sole determinant of topology simplification by type II DNA topoisomerases

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Neil H. Thomson ◽  
Sergio Santos ◽  
Lesley A. Mitchenall ◽  
Tanya Stuchinskaya ◽  
James A. Taylor ◽  
...  
Keyword(s):  
Type Ii ◽  
Dna Topoisomerases ◽  
Type Ii Dna Topoisomerases ◽  
Topology Simplification

DNA crossovers and type II DNA topoisomerases: a thermodynamical study 1 1Edited by T. Richmond

1998 ◽  
Vol 284 (5) ◽  
pp. 1279-1287 ◽  
Author(s):  
Jean-Louis Sikorav ◽  
Bertrand Duplantier ◽  
Gérard Jannink ◽  
Youri Timsit
Keyword(s):  
Type Ii ◽  
Dna Topoisomerases ◽  
Type Ii Dna Topoisomerases

Sequence-specific poisons of type II DNA topoisomerases

1998 ◽  
pp. 7-38 ◽  
Author(s):  
Giovanni Capranico ◽  
Monica Binaschi ◽  
Maria E. Borgnetto ◽  
Franco Zunino ◽  
Mariagrazia Cornarotti ◽  
...  
Keyword(s):  
Type Ii ◽  
Dna Topoisomerases ◽  
Type Ii Dna Topoisomerases

scholarly journals TOP2B: The First Thirty Years

2018 ◽  
Vol 19 (9) ◽  
pp. 2765 ◽  
Author(s):  
Caroline Austin ◽  
Ka Lee ◽  
Rebecca Swan ◽  
Mushtaq Khazeem ◽  
Catriona Manville ◽  
...  
Keyword(s):  
Dependent Manner ◽  
Type Ii ◽  
Dna Duplex ◽  
Topoisomerase Iiα ◽  
Dna Topoisomerase ◽  
Dna Topoisomerases ◽  
Cellular Processes ◽  
Double Stranded Dna ◽  
Type Ii Dna Topoisomerases ◽  
Strand Passage

Type II DNA topoisomerases (EC 5.99.1.3) are enzymes that catalyse topological changes in DNA in an ATP dependent manner. Strand passage reactions involve passing one double stranded DNA duplex (transported helix) through a transient enzyme-bridged break in another (gated helix). This activity is required for a range of cellular processes including transcription. Vertebrates have two isoforms: topoisomerase IIα and β. Topoisomerase IIβ was first reported in 1987. Here we review the research on DNA topoisomerase IIβ over the 30 years since its discovery.

Epitope Distribution of Randomly Established Monoclonal Antibodies against Human Type II DNA Topoisomerases

2002 ◽  
Vol 132 (3) ◽  
pp. 409-416 ◽  
Author(s):  
A. Sakaguchi ◽  
M. Miyaike ◽  
K. Kuroda ◽  
N. Nozaki ◽  
M. Tanaka ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Type Ii ◽  
Dna Topoisomerases ◽  
Human Type ◽  
Type Ii Dna Topoisomerases

scholarly journals Hot-spot consensus of fluoroquinolone-mediated DNA cleavage by Gram-negative and Gram-positive type II DNA topoisomerases

2007 ◽  
Vol 35 (18) ◽  
pp. 6075-6085 ◽  
Author(s):  
S. N. Richter ◽  
G. Giaretta ◽  
V. Comuzzi ◽  
E. Leo ◽  
L. A. Mitchenall ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Hot Spot ◽  
Type Ii ◽  
Gram Positive ◽  
Dna Topoisomerases ◽  
Gram Negative ◽  
Positive Type ◽  
Type Ii Dna Topoisomerases
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