scholarly journals Mechanism of topology simplification by type II DNA topoisomerases

2001 ◽  
Vol 98 (6) ◽  
pp. 3045-3049 ◽  
Author(s):  
A. V. Vologodskii ◽  
W. Zhang ◽  
V. V. Rybenkov ◽  
A. A. Podtelezhnikov ◽  
D. Subramanian ◽  
...  
2010 ◽  
Vol 38 (2) ◽  
pp. 438-442 ◽  
Author(s):  
Andrew D. Bates ◽  
Anthony Maxwell

Type II DNA topoisomerases catalyse changes in DNA topology in reactions coupled to the hydrolysis of ATP. In the case of DNA gyrase, which can introduce supercoils into DNA, the requirement for free energy is clear. However, the non-supercoiling type II enzymes carry out reactions that are apparently energetically favourable, so their requirement for ATP hydrolysis is not so obvious. It has been shown that many of these enzymes (the type IIA family) can simplify the topology of their DNA substrates to a level beyond that expected at equilibrium. Although this seems to explain their usage of ATP, we show that the free energies involved in topology simplification are very small (<0.2% of that available from ATP) and we argue that topology simplification may simply be an evolutionary relic.


2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Neil H. Thomson ◽  
Sergio Santos ◽  
Lesley A. Mitchenall ◽  
Tanya Stuchinskaya ◽  
James A. Taylor ◽  
...  

1998 ◽  
Vol 284 (5) ◽  
pp. 1279-1287 ◽  
Author(s):  
Jean-Louis Sikorav ◽  
Bertrand Duplantier ◽  
Gérard Jannink ◽  
Youri Timsit

Author(s):  
Giovanni Capranico ◽  
Monica Binaschi ◽  
Maria E. Borgnetto ◽  
Franco Zunino ◽  
Mariagrazia Cornarotti ◽  
...  

2018 ◽  
Vol 19 (9) ◽  
pp. 2765 ◽  
Author(s):  
Caroline Austin ◽  
Ka Lee ◽  
Rebecca Swan ◽  
Mushtaq Khazeem ◽  
Catriona Manville ◽  
...  

Type II DNA topoisomerases (EC 5.99.1.3) are enzymes that catalyse topological changes in DNA in an ATP dependent manner. Strand passage reactions involve passing one double stranded DNA duplex (transported helix) through a transient enzyme-bridged break in another (gated helix). This activity is required for a range of cellular processes including transcription. Vertebrates have two isoforms: topoisomerase IIα and β. Topoisomerase IIβ was first reported in 1987. Here we review the research on DNA topoisomerase IIβ over the 30 years since its discovery.


2002 ◽  
Vol 132 (3) ◽  
pp. 409-416 ◽  
Author(s):  
A. Sakaguchi ◽  
M. Miyaike ◽  
K. Kuroda ◽  
N. Nozaki ◽  
M. Tanaka ◽  
...  

2007 ◽  
Vol 35 (18) ◽  
pp. 6075-6085 ◽  
Author(s):  
S. N. Richter ◽  
G. Giaretta ◽  
V. Comuzzi ◽  
E. Leo ◽  
L. A. Mitchenall ◽  
...  

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