An amino acid liquid synthetic medium for the development of mycellal and yeast forms ofCandida albicans

1975 ◽  
Vol 13 (2) ◽  
pp. 148-153 ◽  
Author(s):  
K.L. Lee ◽  
Helen R. Buckley ◽  
Charlotte C. Campbell
Keyword(s):  
Amino Acid ◽  
Synthetic Medium ◽  
Ofcandida Albicans ◽  
Liquid Synthetic Medium

THE NUTRITION OF VIBRIO COSTICOLUS: I. A SIMPLIFIED SYNTHETIC MEDIUM

1962 ◽  
Vol 8 (6) ◽  
pp. 923-928 ◽  
Author(s):  
W. L. Flannery ◽  
Dianne M. Kennedy
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Sodium Chloride ◽  
Sodium Nitrate ◽  
Growth Response ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Maximum Growth ◽  
Potassium Phosphate Buffer ◽  
Liquid Synthetic Medium

A liquid synthetic medium is described in which Vibrio costicolus grows well in 24 hours. The medium contains glucose, L-cystine, L-glutamic acid, L-arginine, DL-valine, DL-isoleucine, potassium phosphate buffer, magnesium sulphate, sodium nitrate, and sodium chloride. The addition of 10 vitamins, 3 bases, 14 amino acids, and 5 salts did not improve the medium. No growth was obtained if cystine, glucose, or sodium chloride was omitted from the medium. The remaining constituents of the simplified medium were not essential but were found necessary for maximum growth. An amino acid antagonism was observed between valine and isoleucine. When added alone, either amino acid depressed the growth response, but when added together they were stimulatory.

THE METABOLISM OF ANIMAL TISSUES CULTIVATED IN VITRO: II. AMINO ACID METABOLISM OF CHICK EMBRYONIC KIDNEY, CHICK EMBRYONIC LIVER, AND MONKEY KIDNEY CORTEX CULTURES

1958 ◽  
Vol 36 (1) ◽  
pp. 171-184 ◽  
Author(s):  
Arthur E. Pasieka ◽  
Helen J. Morton ◽  
Joseph F. Morgan
Keyword(s):  
Tissue Culture ◽  
Amino Acid ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Amino Acid Uptake ◽  
Monkey Kidney ◽  
Kidney Cortex ◽  
Acid Uptake ◽  
Embryonic Liver

Freshly-explanted chick embryonic kidney, chick embryonic liver, and trypsinized monkey kidney cortex cells have been cultivated in vitro in completely synthetic medium M 150. The amino acid changes in the nutrient medium during cultivation of these tissues have been studied by paper chromatography. A characteristic pattern of amino acid uptake and accumulation in the used culture medium has been demonstrated with each type of tissue culture. It has also been shown that, while the amino acid changes in the medium are different with each type of tissue culture, all cultures examined removed adenine from the medium and liberated small amounts of material thought to be hypoxanthine.

scholarly journals Targeted Disruption of a Fungal G-Protein β Subunit Gene Results in Increased Vegetative Growth but Reduced Virulence

1997 ◽  
Vol 10 (8) ◽  
pp. 984-993 ◽  
Author(s):  
Shin Kasahara ◽  
Donald L. Nuss
Keyword(s):  
Amino Acid ◽  
G Protein ◽  
Vegetative Growth ◽  
Synthetic Medium ◽  
Cryphonectria Parasitica ◽  
Β Subunit ◽  
Subunit Gene ◽  
Targeted Disruption ◽  
Fungal Virulence ◽  
Α Subunit

Targeted disruption of two G-protein α subunit genes in the chestnut blight fungus Cryphonectria parasitica revealed roles for the Giα subunit CPG-1 in fungal reproduction, virulence, and vegetative growth. A second Gα subunit, CPG-2, was found to be dispensable for these functions. We now report the cloning and targeted disruption of a C. parasitica G-protein β subunit gene. The deduced amino acid sequence encoded by this gene, designated cpgb-1, was found to share 66.2, 65.9, and 66.7% amino acid identity with Gβ homologues from human, Drosophila, and Dictyostelium origins, respectively, but only 39.7% identity with the Saccharomyces cerevisiae Gβ homologue STE4 product. Low stringency Southern hybridization failed to detect any related Gβ subunit genes in C. parasitica. Targeted disruption of cpgb-1 resulted in several of the changes previously reported to accompany disruption of the C. parasitica Giα subunit gene cpg-1. These included very significant reductions in pigmentation, asexual sporulation, and virulence. In contrast to results obtained for Giα gene disruption, the reduction in virulence resulting from the disruption of a Gβ gene was accompanied by increased, rather than decreased, vegetative growth on synthetic medium. The relevance of these results to mechanisms of fungal virulence is considered.

scholarly journals The effect of farnesol on amino acid incorporation by a wild-type and cell-wall variant strain ofCandida albicans

2005 ◽  
Vol 51 (8) ◽  
pp. 715-718 ◽  
Author(s):  
Phyllis C Braun
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Amino Acid ◽  
Quorum Sensing ◽  
Cell Surface ◽  
Wild Type ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Ofcandida Albicans ◽  
Density Growth

Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 µg/mL in yeast nitrogen broth. Farnesol concentrations greater than 10 µg/mL abolished the enhanced incorporation, and the magnitude of the increased incorporation was dependent on cell-surface hydrophobicity.Key words: Candida albicans, farnesol, amino acid incorporation.

Components in brain heart infusion selective for chromogenic variants of Staphylococcus aureus

1970 ◽  
Vol 16 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Joseph T. Parisi ◽  
Michael P. Kiley
Keyword(s):  
Amino Acids ◽  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Chromatographic Analysis ◽  
Synthetic Medium ◽  
Brain Heart Infusion ◽  
Large Numbers ◽  
Complete Synthetic Medium ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Large numbers of chromogenic variants were isolated from cultures of a parent strain of Staphylococcus aureus growing in the dialysate but not in the residue of brain heart infusion (Difco). Gas–liquid chromatographic analysis of the dialysate detected 18 amino acids in this medium. Large numbers of chromogenic variants also were isolated from 13 of 18 synthetic media deficient in a single amino acid but not in the complete synthetic medium containing all 18 amino acids. Gas–liquid chromatographic analysis detected marked quantitative differences in the amino acid metabolites present in a complete synthetic medium and the synthetic medium deficient in arginine after growth for 12 days. The data suggest that differences in the amino acid metabolism of the parent and chromogenic variants could account for the population changes observed in brain heart infusion.

Toxicity of free riboflavin and methionine–riboflavin solutions to Phytophthora infestans and the reduction of potato late blight disease

1992 ◽  
Vol 38 (11) ◽  
pp. 1108-1111 ◽  
Author(s):  
C. M. Jordan ◽  
R. J. Wakeman ◽  
J. E. DeVay
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Synthetic Medium ◽  
Hyphal Growth ◽  
Fluorescent Light ◽  
Potato Plants ◽  
Contrast Exposure ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Liquid Synthetic Medium

Treatments of sporangia and zoospores of Phytophthora infestans race 1.2.3.4 with methionine or riboflavin for durations of up to 8 h under fluorescent light did not affect its colonization of rye-seed agar. In contrast, exposure of sporangia and zoospores to methionine-riboflavin mixture for 2 h or more resulted in the failure of race 1.2.3.4 to grow when transferred to rye-seed agar medium. Hyphal growth of races 1.2.3.4 and 0, when incubated in liquid synthetic medium, was inhibited by free riboflavin. Potato plants, Solanum tuberosum cv. Kennebec, when pretreated with a methionine–riboflavin mixture and then spray inoculated with race 1.2.3.4 developed fewer diseased leaves per plant than plants pretreated with water, methionine, or riboflavin. Key words: Solanum tuberosum, hydroxyl radical, superoxide anion.

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