Isolation and Identification of Phosphate Solubilizing Bacillus spp. from Tamarix ramosissima Rhizosphere and Their Effect on Growth of Phaseolus vulgaris Under Salinity Stress

2020 ◽  
Vol 37 (10) ◽  
pp. 901-908 ◽  
Author(s):  
Ali Abdelmoteleb ◽  
D. Gonzalez-Mendoza
Keyword(s):  
Phaseolus Vulgaris ◽  
Salinity Stress ◽  
Tamarix Ramosissima ◽  
Isolation And Identification ◽  
Bacillus Spp ◽  
Phosphate Solubilizing ◽  
Effect On Growth

scholarly journals Isolation and Identification of Uranium Tolerant Phosphate-Solubilizing Bacillus spp. and Their Synergistic Strategies to U(VI) Immobilization

2021 ◽  
Vol 12 ◽  
Author(s):  
Juan Zhong ◽  
Xuewu Hu ◽  
Xingyu Liu ◽  
Xinglan Cui ◽  
Ying Lv ◽  
...  
Keyword(s):  
Extracellular Polymeric Substances ◽  
Rrna Genes ◽  
Phosphate Solubilizing Bacteria ◽  
Ftir Analysis ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Interesting Phenomenon ◽  
Bacterial Surface ◽  
Bacillus Spp ◽  
Phosphate Solubilizing

The remediation of uranium (U) through phosphate-solubilizing bacteria (PSB) is an emerging technique as well as an interesting phenomenon for transforming mobile U into stable minerals in the environment. While studies are well needed for in-depth understanding of the mechanism of U(VI) immobilization by PSB. In this study, two PSB were isolated from a U-tailing repository site. These bacterial strains (ZJ-1 and ZJ-3) were identified as Bacillus spp. by the sequence analysis of 16S ribosomal RNA (rRNA) genes. Incubation of PSB in liquid medium showed that the isolate ZJ-3 could solubilize more than 230 mg L–1 P from glycerol-3-phosphate and simultaneously removed over 70% of 50 mg L–1 U(VI) within 1 h. During this process, the rapid appearance of yellow precipitates was observed. The microscopic and spectroscopic analysis demonstrated that the precipitates were associated with U-phosphate compound in the form of saleeite-like substances. Besides, scanning electron microscopy coupled with energy-dispersive X-ray (SEM-EDS) and Fourier transform infrared spectroscopy (FTIR) analysis of the precipitates confirmed that the extracellular polymeric substances (EPS) might also play a key role in U sequestration. Furthermore, SEM and FTIR analysis revealed that part of U(VI) was adsorbed on the bacterial surface through cellular phosphate, hydroxy, carboxyl, and amide groups. This study provides new insights into the synergistic strategies enhancing U immobilization rates by Bacillus spp. that uses glycerol-3-phosphate as the phosphorus source, the process of which contributes to harmful pollutant biodegradation.

Nodulation ofPhaseolus vulgarisbyRhizobium etliis enhanced by the presence ofBacillus

1997 ◽  
Vol 43 (1) ◽  
pp. 1-8 ◽  
Author(s):  
M. Srinivasan ◽  
F. B. Holl ◽  
D. J. Petersen
Keyword(s):  
Phaseolus Vulgaris ◽  
Lateral Root ◽  
Root Formation ◽  
Bacterial Species ◽  
Plant Roots ◽  
Rhizobium Etli ◽  
Phaseolus Acutifolius ◽  
Plant Interaction ◽  
Split Root ◽  
Bacillus Spp

The ability of Bacillus spp. to alter the nodulation of Phaseolus vulgaris by Rhizobium etli was assessed. The simultaneous presence of both Rhizobium etli TAL 182 and Bacillus megaterium S49 on plant roots during the early stages of plant growth was necessary for enhanced nodulation of Phaseolus vulgaris by the Rhizobium microsymbiont. Coinoculation with both bacterial species also facilitated heterologous nodulation of Rhizobium TAL 182 on Phaseolus acutifolius. These results are consistent with earlier reports of increased root hair proliferation and lateral root formation in response to coinoculation. Split-root experiments revealed that coinoculation partially suppressed host-controlled regulation of nodulation, implicating a plant interaction with the two bacterial species. Changes to the nodulation potential of R. etli due to coinoculation with Bacillus spp. demonstrate the potential for root-associated organisms other than rhizobia to alter the dynamics of the legume–Rhizobium symbiosis.Key words: Bacillus, nodulation enhancement, heterologous nodulation.

scholarly journals Potencial in vitro de Bacillus spp. no controle de Curtobacterium flaccumfaciens pv. flaccumfaciens em feijoeiro-comum

2016 ◽  
Vol 42 (4) ◽  
pp. 360-362
Author(s):  
Evelynne Urzêdo Leão ◽  
◽  
Júlio Cesar da Silva ◽  
Fabíola Rodrigues Medeiros ◽  
Gabriela Silva Santa Rosa Macêdo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Bacillus Licheniformis ◽  
Bacillus Spp

RESUMO O feijoeiro-comum (Phaseolus vulgaris) é uma cultura de grande relevância na alimentação da população brasileira. A murcha-de-curtobacterium ou murcha bacteriana, causada por Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff). é uma doença vascular que acomete o feijoeiro causando danos significativos. Neste contexto, o objetivo deste trabalho foi avaliar a ação in vitro de Bacillus spp. na inibição de dois isolados de Cff, colonização do sistema radicular e desenvolvimento de plântulas de feijoeiro-comum. Foram realizados dois ensaios in vitro para verificar a atividade antagônica dos isolados Bacillus licheniformis, B. subtilis e B. subtilis + B. lichenformis a dois isolados de Cff. Todos os isolados de Bacillus spp. apresentaram inibição no crescimento dos isolados de Cff. Não foi observada a colonização das raízes das plântulas de feijoeiro-comum, pelos isolados bacterianos avaliados.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Potential Piriformospora indica effect on growth and mineral nutrition of Phaseolus vulgaris crop under low phosphorus intake

2020 ◽  
Vol 44 (4) ◽  
pp. 498-507
Author(s):  
Hayet Beltayef ◽  
Mongi Melki ◽  
Wafa Saidi ◽  
Rim Hajri ◽  
Cristina Cruz ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Mineral Nutrition ◽  
Piriformospora Indica ◽  
Low Phosphorus ◽  
Phosphorus Intake ◽  
Effect On Growth
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