Antifungal Activity of Some Plant Extracts Against Fungal Pathogens of Tea (Camellia sinensis.)

Background: Fungal infections are one of the common skin diseases with difficulty in their treatment approach. The present efficient drugs for fungal infection are limited. Aim: To determine the therapeutic efficacy of plant extracts as alternative antifungal agents. Materials and methods: 100 clinical samples [68 from female and 32 from male] were collected during the period from March to July 2017 from subjects attending Dermatology Clinic in Salah Uldean General Hospital. Fungal infection was diagnosed with using KOH wet preparation. Fungal species identified by using conventional approach. The active ingredients existing in the plant extracts were detected and analyzed through qualitative and quantitative detection technique of chemical compounds using a high performance liquid chromatographic device (HPLC). Agar diffusion method was used to determine antifungal activity of plant extracts. Results: Direct microscopic examination showed that there were (75%) positive samples, while culture shows (67%) positive samples. The isolated dermatophytes belong to Epidermophyoton, Microsporum, and Trichophyton genus. The predominant dermatophytes were T. rubrum (25%) species. The highest frequency of infection was in the age group of 11-20 years. The sensitivity of the tested fungi to the aqueous and alcoholic plant extracts varies. Alcoholic extract of the hot pepper plant was more effective as antifungal than the aqueous extract of the same plant. However, aqueous hot pepper extracts was more effective against T. mentagrophyte than that of alcoholic extract. Additionally, alcoholic Sumac extract shows higher efficacy that aqueous extract. Conclusion: Hot pepper and Sumac extracts show antifungal activity against Microsporum canis, Trichophyton rubrum and T. mentagrophyte.

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Antifungal activity of medicinal plant extracts for potential management of Fusarium pathogens

Research on Crops ◽

10.31830/2348-7542.2019.058 ◽

2019 ◽

Vol 20 (2) ◽

Keyword(s):

Antifungal Activity ◽

Medicinal Plant ◽

Plant Extracts ◽

Medicinal Plant Extracts ◽

Fusarium Pathogens

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Antifungal activity of plant extracts against dandruff causing organism Malassezia furfur

International Journal of Bioassays ◽

10.21746/ijbio.2016.11.0010 ◽

2016 ◽

Vol 5 (11) ◽

pp. 5047 ◽

Cited By ~ 3

Author(s):

Mamatha Pingili* ◽

Sridhar Vanga ◽

Rama Krishna Raparla

Keyword(s):

Antifungal Activity ◽

Plant Extracts ◽

Malassezia Furfur ◽

Plate Method

In the present study, an attempt was made to know the activity of different plant extracts against the dandruff causing organism Malassezia furfur. Various parts of twelve different plants were collected and their crude as well as powered extracts were used for the activity studies. The evaluation was done using cup plate method. Sensitivity of the organism to marketed antidandruff shampoos was also studied. Of all the extracts, antifungal activity was shown by lemon, amla, shikakai, henna (decreasing order of activity). These results were compared with the antifungal activity of three branded shampoos. On comparison, it is inferred that plant extracts showed promising activity against Malassezia furfur when compared with the marketed antidandruff shampoos.

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Biodirected Synthesis of Silver Nanoparticles Using Aqueous Honey Solutions and Evaluation of Their Antifungal Activity against Pathogenic Candida Spp.

International Journal of Molecular Sciences ◽

10.3390/ijms22147715 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7715

Author(s):

Grzegorz Czernel ◽

Dominika Bloch ◽

Arkadiusz Matwijczuk ◽

Jolanta Cieśla ◽

Monika Kędzierska-Matysek ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antifungal Activity ◽

Fungal Pathogens ◽

Fungal Growth ◽

Disc Diffusion ◽

Candida Spp ◽

Antifungal Effect ◽

Synthesis Of Nanoparticles ◽

Honey Solution ◽

Diffusion Assay

Silver nanoparticles (AgNPs) were synthesized using aqueous honey solutions with a concentration of 2%, 10%, and 20%—AgNPs-H2, AgNPs-H10, and AgNPs-H20. The reaction was conducted at 35 °C and 70 °C. Additionally, nanoparticles obtained with the citrate method (AgNPs-C), while amphotericin B (AmB) and fluconazole were used as controls. The presence and physicochemical properties of AgNPs was affirmed by analyzing the sample with ultraviolet–visible (UV–Vis) and fluorescence spectroscopy, scanning electron microscopy (SEM), and dynamic light scattering (DLS). The 20% honey solution caused an inhibition of the synthesis of nanoparticles at 35 °C. The antifungal activity of the AgNPs was evaluated using opportunistic human fungal pathogens Candida albicans and Candida parapsilosis. The antifungal effect was determined by the minimum inhibitory concentration (MIC) and disc diffusion assay. The highest activity in the MIC tests was observed in the AgNPs-H2 variant. AgNPs-H10 and AgNPs-H20 showed no activity or even stimulated fungal growth. The results of the Kirby–Bauer disc diffusion susceptibility test for C. parapsilosis strains indicated stronger antifungal activity of AgNPs-H than fluconazole. The study demonstrated that the antifungal activity of AgNPs is closely related to the concentration of honey used for the synthesis thereof.

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Protective Antifungal Activity of Plantago major Extract Against the Phytopathogenic Fungi Phytophthora cinnamomi, Diplodia corticola and Colletotrichum Species

Proceedings ◽

10.3390/foods_2020-07678 ◽

2020 ◽

Vol 70 (1) ◽

pp. 94

Author(s):

Cláudia Ferreira ◽

Rui Oliveira

Keyword(s):

Antifungal Activity ◽

Secondary Metabolites ◽

Plant Extracts ◽

Phytophthora Cinnamomi ◽

Fungal Infections ◽

Petri Dish ◽

Phytopathogenic Fungi ◽

Colletotrichum Acutatum ◽

Lower Probability ◽

Plantago Major

Synthetic fungicides for crops protection raise environmental and human concerns due to accumulation in edible vegetables, showing significant toxicity to humans, and in soil, groundwater and rivers, affecting ecological balance. In addition, they are prone to the development of resistant strains because of the single target-based mechanism of action. Plant extracts provide attractive alternatives, as they constitute a rich source of biodegradable secondary metabolites, such as phenols, flavonoids and saponins, which have multiple modes of antifungal action and a lower probability of the development of resistant fungi. This work has the objective of identifying plant extracts with antifungal activity, aiming to contribute to food safety and sustainable agricultural practices. We selected a saponin-containing plant, Plantago major, and extracted secondary metabolites with 50% (v/v) ethanol, dried by evaporation, and dissolved in water. For antifungal activity, the phytopathogenic fungi Colletotrichum acutatum, Colletotrichum gloeosporioides, Colletotrichum godetiae, Colletotrichum nymphaeae, Diplodia corticola and Phytophthora cinnamomi were selected because they affect fruits and vegetables, such as strawberry, almond, apple, avocado, blueberry and chestnut trees. The aqueous extract was incorporated into PDA medium at different concentrations and mycelial discs were placed in the center of each Petri dish. Growth was measured as the radial mycelial growth at 3, 6, and 9 days incubation at 25 °C in the dark. The maximum growth inhibition (32.2%) was obtained against P. cinnamomi with 2000 µg/mL extract followed by C. gloeosporioides (25.7%) on the sixth day and by C. godetiae and C. nymphaeae (21.1%) on the ninth day. Results show that P. major presents antifungal activity in all phytopathogenic fungi tested and the extract can be used to protect important crops, by inhibiting the development of fungal infections and promoting food security and a sustainable agriculture.

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Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

FEMS Microbiology Ecology ◽

10.1093/femsec/fiz138 ◽

2019 ◽

Cited By ~ 6

Author(s):

Maria-Dimitra Tsolakidou ◽

Ioannis A Stringlis ◽

Natalia Fanega-Sleziak ◽

Stella Papageorgiou ◽

Antria Tsalakou ◽

...

Keyword(s):

Antifungal Activity ◽

Fungal Pathogens ◽

Growth Parameters ◽

Tomato Plants ◽

Beneficial Effects ◽

Negative Effect ◽

Tomato Growth ◽

In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

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