A thermogelling organic-inorganic hybrid hydrogel with excellent printability, shape fidelity and cytocompatibility for 3D bioprinting

2021 ◽  
Author(s):  
Chen Hu ◽  
Taufiq Ahmad ◽  
Malik Salman Haider ◽  
Lukas Hahn ◽  
Philipp Stahlhut ◽  
...  
Keyword(s):  
Diblock Copolymer ◽  
Molecular Level ◽  
Three Dimensional ◽  
The Novel ◽  
3D Bioprinting ◽  
Hybrid Hydrogel ◽  
Decellularized Extracellular Matrix ◽  
Ionic Crosslinking ◽  
Hybrid Hydrogels ◽  
Wide Range

Abstract Alginates are the most commonly used bioink in biofabrication, but their rheological profiles makes it very challenging to perform real 3D printing. In this study, an advanced hybrid hydrogel ink was developed, a mixture of thermogelling diblock copolymer, alginate and clay i.e. Laponite XLG. The reversible thermogelling and shear thinning properties of the diblock copolymer in the ink system improves handling and 3D printability significantly. Various three-dimensional constructs, including suspended filaments, were printed successfully with high shape fidelity and excellent stackability. Subsequent ionic crosslinking of alginate fixates the printed scaffolds, while the diblock copolymer is washed out of the structure, acting as a fugitive material on the (macro)molecular level. Finally, cell-laden printing and culture over 21 days demonstrated good cytocompatibility and feasibility of the novel hybrid hydrogels for 3D bioprinting. We believe that the developed material could be interesting for a wide range of bioprinting applications including tissue engineering and drug screening, potentially enabling also other biological bioinks such as collagen, hyaluronic acid, decellularized extracellular matrix or cellulose based bioinks.

Self-assembled three-dimensional hierarchical graphene hybrid hydrogels with ultrathin β-MnO2 nanobelts for high performance supercapacitors

2015 ◽  
Vol 3 (4) ◽  
pp. 1540-1548 ◽  
Author(s):  
Sheng Zhu ◽  
Hui Zhang ◽  
Ping Chen ◽  
Lin-Hui Nie ◽  
Chuan-Hao Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
High Performance ◽  
Three Dimensional ◽  
The Self ◽  
Mass Loading ◽  
Hybrid Hydrogel ◽  
Precise Control ◽  
Ultrafine Structure ◽  
Hybrid Hydrogels ◽  
Self Assembled

A facile protocol for the self-assembly of the rGO/β-MnO2 hybrid hydrogel with ultrafine structure and precise control of mass-loading for high performance supercapacitors is reported.

scholarly journals Aspiration-assisted bioprinting for precise positioning of biologics

2020 ◽  
Vol 6 (10) ◽  
pp. eaaw5111 ◽  
Author(s):  
Bugra Ayan ◽  
Dong Nyoung Heo ◽  
Zhifeng Zhang ◽  
Madhuri Dey ◽  
Adomas Povilianskas ◽  
...  
Keyword(s):  
Self Assembly ◽  
Physical Mechanism ◽  
Single Cells ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Precise Control ◽  
3D Space ◽  
Magnitude Range ◽  
Wide Range ◽  
Order Of Magnitude

Three-dimensional (3D) bioprinting is an appealing approach for building tissues; however, bioprinting of mini-tissue blocks (i.e., spheroids) with precise control on their positioning in 3D space has been a major obstacle. Here, we unveil “aspiration-assisted bioprinting (AAB),” which enables picking and bioprinting biologics in 3D through harnessing the power of aspiration forces, and when coupled with microvalve bioprinting, it facilitated different biofabrication schemes including scaffold-based or scaffold-free bioprinting at an unprecedented placement precision, ~11% with respect to the spheroid size. We studied the underlying physical mechanism of AAB to understand interactions between aspirated viscoelastic spheroids and physical governing forces during aspiration and bioprinting. We bioprinted a wide range of biologics with dimensions in an order-of-magnitude range including tissue spheroids (80 to 600 μm), tissue strands (~800 μm), or single cells (electrocytes, ~400 μm), and as applications, we illustrated the patterning of angiogenic sprouting spheroids and self-assembly of osteogenic spheroids.

scholarly journals Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2203 ◽  
Author(s):  
JunJie Yu ◽  
SuJeong Lee ◽  
Sunkyung Choi ◽  
Kee K. Kim ◽  
Bokyeong Ryu ◽  
...  
Keyword(s):  
Three Dimensional ◽  
The Novel ◽  
Osteochondral Defects ◽  
3D Bioprinting ◽  
Hybrid Scaffold ◽  
Osteochondral Tissue ◽  
Biological Performance ◽  
Osteochondral Tissue Engineering ◽  
Made In

Osteochondral defects, including damage to both the articular cartilage and the subchondral bone, are challenging to repair. Although many technological advancements have been made in recent years, there are technical difficulties in the engineering of cartilage and bone layers, simultaneously. Moreover, there is a great need for a valuable in vitro platform enabling the assessment of osteochondral tissues to reduce pre-operative risk. Three-dimensional (3D) bioprinting systems may be a promising approach for fabricating human tissues and organs. Here, we aimed to develop a polycaprolactone (PCL)/alginate bipartite hybrid scaffold using a multihead 3D bioprinting system. The hybrid scaffold was composed of PCL, which could improve the mechanical properties of the construct, and alginate, encapsulating progenitor cells that could differentiate into cartilage and bone. To differentiate the bipartite hybrid scaffold into osteochondral tissue, a polydimethylsiloxane coculture system for osteochondral tissue (PCSOT) was designed and developed. Based on evaluation of the biological performance of the novel hybrid scaffold, the PCL/alginate bipartite scaffold was successfully fabricated; importantly, our findings suggest that this PCSOT system may be applicable as an in vitro platform for osteochondral tissue engineering.

Novel Design of a Three-Dimensional Printed Umbilical Cord Clamp: A Proof of Concept Study

2019 ◽  
Vol 13 (3) ◽  
Author(s):  
Betul Mammadov ◽  
Gulsen Vural ◽  
Ersin Aytac ◽  
Emil Mammadov
Keyword(s):  
Umbilical Cord ◽  
Umbilical Vein ◽  
Human Subjects ◽  
Three Dimensional ◽  
The Novel ◽  
3D Print ◽  
Wide Range ◽  
Fluid Leak ◽  
Umbilical Cords ◽  
Novel Design

Parents frequently report complaints regarding the excessive size of the clamp that create concerns of hooking to the clothes and inducing premature separation of the dried cord. We aimed to design three-dimensional (3D) print and test a novel umbilical clamp which would be smaller, more ergonomic, and would look more appealing to the parents. After the design and 3D printing process, the clamps were tested for fluid leak on fresh umbilical cord specimens. The umbilical vein was cannulated with a 4F feeding tube which was attached to the saline infusion bag inserted in a pressure infusion cuff. The compressed cord width was measured, and the cuff was inflated to 100 mmHg and 200 mmHg. Umbilical cord specimens of 152 patients were included in the main study. The mean umbilical cord diameter (UCD) was 11.1 ± 3.5 mm (range: 4.5–23.3 mm). The compressed cord width mean value after clamping with the novel design clamp was 17.3 ± 2.3 mm compared to the 23.5 ± 3.5 mm with the conventional clamp and this difference was statistically significant (p < 0.001). All of the novel clamps safely and efficiently clamped the umbilical cords without any fluid leak. The novel design umbilical cord clamp is a safe and efficient tool being able to clamp a wide range of umbilical cords. However, a prospective randomized trial on human subjects, safety-efficacy tests by independent healthcare providers, and patient satisfaction surveys are mandatory for the further approval of our claims.

pH- and electro-response characteristics of bacterial cellulose nanofiber/sodium alginate hybrid hydrogels for dual controlled drug delivery

RSC Advances ◽  
2014 ◽  
Vol 4 (87) ◽  
pp. 47056-47065 ◽  
Author(s):  
Xiangning Shi ◽  
Yudong Zheng ◽  
Guojie Wang ◽  
Qinghua Lin ◽  
Jinsheng Fan
Keyword(s):  
Sodium Alginate ◽  
Ph Value ◽  
Stimuli Responsive ◽  
Drug Release Rate ◽  
Hybrid Hydrogel ◽  
Electric Stimulus ◽  
Response Characteristics ◽  
Ionic Crosslinking ◽  
Hybrid Hydrogels

An ionic crosslinking nanocellulose/sodium alginate (BC/SA) hybrid hydrogel was prepared as a dual-stimuli responsive release system. The drug release rate of BC/SA hybrid hydrogels in vitro not only depend on pH value but also depend on the presence of electric stimulus.

Preparation of Continuous Al2O3/Y2O3 Coating on Carbon Fiber by a Novel Aqueous Plasma Electrolysis

2017 ◽  
Vol 898 ◽  
pp. 1575-1582 ◽  
Author(s):  
Yu Ping Zhang ◽  
Yong Hua Shen ◽  
Wei Wei Chen ◽  
Huan Wu Cheng ◽  
Lu Wang
Keyword(s):  
Carbon Fiber ◽  
Fiber Bundle ◽  
Three Dimensional ◽  
Isothermal Oxidation ◽  
The Novel ◽  
Plasma Arc ◽  
Plasma Electrolysis ◽  
Wide Range ◽  
Carbon Fiber Reinforced Composites ◽  
Coated Carbon

A novel aqueous plasma electrolysis applied for depositing a uniform and continuous Al2O3/Y2O3 ceramic coating on each fiber within a carbon fiber bundle are reported in this study. All equally spaced tungsten wires generated plasma arc, where the plasma was overlapped, excluded and extruded each other, finally a cylindrical plasma arc zone was formed, promoting the full coverage of the coating on each fiber. The Al2O3/Y2O3 coating protected the carbon fiber from oxidation above 850oC. Similarly, the coating significantly improved the oxidation resistance of the carbon fiber with the mass loss of ~5% after isothermal oxidation in N2 atmosphere at 800°C for 30 min, compared to ~14% of bare carbon fiber. By the movement of the T300 carbon fiber bundle, 1500 m coated carbon fiber bundle with the high-quality Al2O3/Y2O3 coating deposited on each fiber was successfully realized. It is believed that the novel aqueous plasma electrolysis will find a wide range of applications in modifying the interface of the carbon fiber reinforced composites. The present investigation will also open up a new horizon for preparing a protective coating on each fiber within two/three-dimensional carbon fiber fabric.

Nanocomposites: A Review on Current Status

2021 ◽  
pp. 231-237
Author(s):  
Nikita D. Gidde ◽  
Manojkumar M. Nitalikar ◽  
Indrayani D. Raut
Keyword(s):  
Amorphous Materials ◽  
Size Range ◽  
Molecular Level ◽  
Three Dimensional ◽  
One Dimension ◽  
Current Status ◽  
One Dimensional ◽  
Wide Range ◽  
Modification Of Materials ◽  
Level Analysis

The atomic and molecular level analysis of controlling matter in nanotechnology deals with structures and components with a size ranging from 1 to 100 nanometers in at least one dimension, and includes the development or modification of materials or devices within that size range. The development of new materials with one dimension at the nanoscale is one of the fundamental aspects of nanotechnology. These materials, referred to as nanomaterials, are nanoscale engineered and have radically different properties than their "bulk" equivalents. The concept of nanocomposites has greatly extended to include a wide range of systems, including one-dimensional, two-dimensional, three-dimensional and amorphous materials, composed of distinctly different components and mixed at the scale of the nanometer.

Exploring siRNA Umpired Nanogels: A Tale of Barrier Combating Carrier

2020 ◽  
Vol 26 (27) ◽  
pp. 3234-3250
Author(s):  
Sushil K. Kashaw ◽  
Prashant Sahu ◽  
Vaibhav Rajoriya ◽  
Pradeep Jana ◽  
Varsha Kashaw ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Biomedical Engineering ◽  
Viral Infections ◽  
Slow Release ◽  
Molecular Level ◽  
Release Kinetics ◽  
Biologically Active ◽  
Release Process ◽  
Rapid Release ◽  
Wide Range

Potential short interfering RNAs (siRNA) modulating gene expression have emerged as a novel therapeutic arsenal against a wide range of maladies and disorders containing cancer, viral infections, bacterial ailments and metabolic snags at the molecular level. Nanogel, in the current medicinal era, displayed a comprehensive range of significant drug delivery prospects. Biodegradation, swelling and de-swelling tendency, pHsensitive drug release and thermo-sensitivity are some of the renowned associated benefits of nanogel drug delivery system. Global researches have also showed that nanogel system significantly targets and delivers the biomolecules including DNAs, siRNA, protein, peptides and other biologically active molecules. Biomolecules delivery via nanogel system explored a wide range of pharmaceutical, biomedical engineering and agro-medicinal application. The siRNAs and DNAs delivery plays a vivacious role by addressing the hitches allied with chronic and contemporary therapeutic like generic possession and low constancy. They also incite release kinetics approach from slow-release while mingling to rapid release at the targets will be beneficial as interference RNAs delivery carriers. Therefore, in this research, we focused on the latest improvements in the delivery of siRNA loaded nanogels by enhancing the absorption, stability, sensitivity and combating the hindrances in cellular trafficking and release process.

Reading Beyond the Code

2018 ◽  
Keyword(s):  
Relevance Theory ◽  
Literary Studies ◽  
The Novel ◽  
Human Communication ◽  
Communicative Acts ◽  
Face To Face ◽  
Code Model ◽  
Wide Range ◽  
Central Claim

This book explores the value for literary studies of relevance theory, an inferential approach to communication in which the expression and recognition of intentions plays a major role. Drawing on a wide range of examples from lyric poetry and the novel, nine of the ten chapters are written by literary specialists and use relevance theory both as an overall framework and as a resource for detailed analysis. The final chapter, written by the co-founder of relevance theory, reviews the issues addressed by the volume and explores their implications for cognitive theories of how communicative acts are interpreted in context. Originally designed to explain how people understand each other in everyday face-to-face exchanges, relevance theory—described in an early review by a literary scholar as ‘the makings of a radically new theory of communication, the first since Aristotle’s’—sheds light on the whole spectrum of human modes of communication, including literature in the broadest sense. Reading Beyond the Code is unique in using relevance theory as a prime resource for literary study, and is also the first to apply the model to a range of phenomena widely seen as supporting an ‘embodied’ conception of cognition and language where sensorimotor processes play a key role. This broadened perspective serves to enhance the value for literary studies of the central claim of relevance theory: that the ‘code model’ is fundamentally inadequate to account for human communication, and in particular for the modes of communication that are proper to literature.

Turbulent three-dimensional dielectric electrohydrodynamic convection between two plates

2012 ◽  
Vol 696 ◽  
pp. 228-262 ◽  
Author(s):  
A. Kourmatzis ◽  
J. S. Shrimpton
Keyword(s):  
Spatial Data ◽  
Three Dimensional ◽  
Reynolds Numbers ◽  
Two Dimensions ◽  
Three Dimensions ◽  
Energy Budgets ◽  
Turbulent Regime ◽  
Scalar Flux ◽  
Wide Range ◽  
Scalar Variance

AbstractThe fundamental mechanisms responsible for the creation of electrohydrodynamically driven roll structures in free electroconvection between two plates are analysed with reference to traditional Rayleigh–Bénard convection (RBC). Previously available knowledge limited to two dimensions is extended to three-dimensions, and a wide range of electric Reynolds numbers is analysed, extending into a fully inherently three-dimensional turbulent regime. Results reveal that structures appearing in three-dimensional electrohydrodynamics (EHD) are similar to those observed for RBC, and while two-dimensional EHD results bear some similarities with the three-dimensional results there are distinct differences. Analysis of two-point correlations and integral length scales show that full three-dimensional electroconvection is more chaotic than in two dimensions and this is also noted by qualitatively observing the roll structures that arise for both low (${\mathit{Re}}_{E} = 1$) and high electric Reynolds numbers (up to ${\mathit{Re}}_{E} = 120$). Furthermore, calculations of mean profiles and second-order moments along with energy budgets and spectra have examined the validity of neglecting the fluctuating electric field ${ E}_{i}^{\ensuremath{\prime} } $ in the Reynolds-averaged EHD equations and provide insight into the generation and transport mechanisms of turbulent EHD. Spectral and spatial data clearly indicate how fluctuating energy is transferred from electrical to hydrodynamic forms, on moving through the domain away from the charging electrode. It is shown that ${ E}_{i}^{\ensuremath{\prime} } $ is not negligible close to the walls and terms acting as sources and sinks in the turbulent kinetic energy, turbulent scalar flux and turbulent scalar variance equations are examined. Profiles of hydrodynamic terms in the budgets resemble those in the literature for RBC; however there are terms specific to EHD that are significant, indicating that the transfer of energy in EHD is also attributed to further electrodynamic terms and a strong coupling exists between the charge flux and variance, due to the ionic drift term.

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