scholarly journals The Combination of Dietary Branched-Chain and Limiting Amino Acids Modulates Feed Intake Through Both Peripheral and Central Mechanisms in Pigs Fed with Low Protein Diets

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 686-686
Author(s):  
Mohammad Habibi ◽  
Cedrick Shili ◽  
Julia Sutton ◽  
Parniyan Goodarzi ◽  
Adel Pezeshki
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Feed Intake ◽  
Mrna Abundance ◽  
Nitrogen Excretion ◽  
Serotonin Concentration ◽  
Poor Growth ◽  
Branched Chain ◽  
Limiting Amino Acids ◽  
Plasma Serotonin

Abstract Objectives Due to its negative influence on human health and environment, excess nitrogen excretion is challenging the sustainability of swine industry. Severe reduction of dietary protein may reduce the excretion of nitrogen from swine production, but this results in poor growth performance of pigs. The aim of current study was to investigate the effect of supplementing LP diets with both branched-chain amino acids (BCAA; i.e., leucine, isoleucine, valine) and other limiting amino acids (LAA; i.e., lysine, methionine, threonine, tryptophan) at levels higher than suggested requirements on the growth performance and mRNA and protein abundance of markers associated with feed intake in nursery pigs. Methods Forty-eight weaned barrows at 3 weeks of age were individually housed and following 2 weeks of adaptation, randomly assigned to 6 treatments for 4 weeks: 1) positive control: 20% crude protein (CP); 2) negative control (NC): 14% CP; 3) NC + LAA 25% more than suggested levels (L25); 4) NC + LAA 50% more than suggested levels (L50); 5) NC + LAA and BCAA 25% more than suggested levels (LB25); 6) NC + LAA and BCAA 50% more than suggested levels (LB50). At week 4 of starting experimental diets, all pigs were euthanized and blood samples, hypothalamus and jejunum samples collected. The data were analyzed with univariate GLM (SPSS®). Results Final body weight was significantly higher in LB50 than L50. The pigs fed with LB50 and LB25 had higher average daily feed intake than L50 and L25, respectively. The LB50 reduced the plasma serotonin concentration relative to L50 and NC. The overall effect of diet on mRNA abundance of hypothalamic tryptophan hydroxylase 1 (TPH1) and 5-hydroxytryptamine receptor 2B (5HTR2B) was significant, but no differences among groups were detected. The pigs in LB50 and LB25 had higher mRNA abundance of hypothalamic NPY (∼20%) than NC. Conclusions Supplementing the LP diets with increased levels of both dietary BCAA and LAA reduced the plasma serotonin concentration and improved the growth performance and feed intake partly through increased hypothalamic NPY. Funding Sources This work was supported by Animal Health and Production and Animal Products: Improved Nutritional Performance, Growth, and Lactation of Animals and by Hatch project from the USDA National Institute of Food and Agriculture.

scholarly journals Supplementation of branched-chain amino acids to a reduced-protein diet improves growth performance in piglets: involvement of increased feed intake and direct muscle growth-promoting effect

2016 ◽  
Vol 115 (12) ◽  
pp. 2236-2245 ◽  
Author(s):  
Liufeng Zheng ◽  
Hongkui Wei ◽  
Chuanshang Cheng ◽  
Quanhang Xiang ◽  
Jiaman Pang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Feed Intake ◽  
Muscle Growth ◽  
Branched Chain Amino Acids ◽  
Promoting Effect ◽  
Weanling Piglets ◽  
Growth Promoting ◽  
T1 And T2 ◽  
Branched Chain

AbstractThe aim of this study was to investigate whether supplementing branched-chain amino acids (AA) (BCAA) along with a reduced-protein diet increases piglet growth, and whether elevated feed intake and muscle growth-promoting effect contribute to this improvement. In Expt 1, twenty-eight weanling piglets were randomly fed one of the following four diets: a positive control (PC) diet, a reduced-protein negative control (NC) diet, an NC diet supplemented with BCAA to the same levels as in the PC diet (test 1 (T1)) and an NC diet supplemented with a 2-fold dose of BCAA in T1 diet (test 2 (T2)) for 28 d. In Expt 2, twenty-one weanling piglets were randomly assigned to NC, T1 and pair-fed T1 (P) groups. NC and T1 diets were the same as in Expt 1, whereas piglets in the P group were individually pair-fed with the NC group. In Expt 1, the NC group had reduced piglet growth and feed intake compared with the PC group, which were restored in T1 and T2 groups, but no differences were detected between T1 and T2 groups. In Expt 2, T1 and P groups showed increases in growth and mass of some muscles compared with the NC group. Increased feed intake after BCAA supplementation was associated with increased mRNA expressions of agouti-related peptide and co-express neuropeptide Y (NPY) and phosphorylation of mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase 1 (S6K1), as well as decreased mRNA expressions of melanocortin-4 receptor and cocaine- and amphetamine-regulated transcript and phosphorylation of eukaryotic initiation factor 2α in the hypothalamus. No differences were observed among PC, T1 and T2 groups except for higher NPY mRNA expression in the T2 group than in the PC group (Expt 1). Phosphorylation of mTOR and S6K1 in muscle was enhanced after BCAA supplementation, which was independent of change in feed intake (Expt 2). In conclusion, supplementing BCAA to reduced-protein diets increases feed intake and muscle mass, and contributes to better growth performance in piglets.

scholarly journals Branched-chain amino acid interactions in growing pig diets1

2019 ◽  
Vol 3 (4) ◽  
pp. 1246-1253 ◽  
Author(s):  
Henrique S Cemin ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Steve S Dritz ◽  
Joel M DeRouchey ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Growth Performance ◽  
Feed Intake ◽  
Negative Impact ◽  
Amino Acid Profile ◽  
Inhibitory Effect ◽  
Keto Acid ◽  
Branched Chain ◽  
The Impact

Abstract The branched-chain amino acids (BCAA) Leu, Ile, and Val share the first steps of their catabolism due to similarities in their structure. The BCAA are reversibly transaminated in skeletal muscle through the activity of branched-chain aminotransferase and then transported to the liver. They undergo an irreversible decarboxylation catalyzed by the branched-chain α-keto acid dehydrogenase complex. Both enzymes are common to Leu, Ile, and Val and increased enzymatic activity stimulated by an excess of one of them will increase the catabolism of all BCAA, which can result in antagonisms. Leucine and its keto acid are the most potent stimulators of BCAA catabolic enzymes. Moreover, BCAA and large neutral amino acids (LNAA) share common brain transporters. Research has shown that high concentrations of BCAA, especially Leu, can decrease the absorption of LNAA, such as Trp, which is a precursor of serotonin and can have a significant impact in feed intake regulation. Finally, high Leu concentrations have the ability to overstimulate the mTOR signaling pathway, resulting in an inhibitory effect on feed intake. Most of the research conducted to evaluate the impact of BCAA on growth performance of pigs seems to agree that high levels of Leu decrease weight gain, mostly due to a reduction in feed intake. However, some studies, mostly with finishing pigs, observed no evidence for an impact on growth performance even with extremely high levels of Leu. It could be hypothesized that these inconsistencies are driven by the entire dietary amino acid profile as opposed to only considering the level of Leu. Grow-finish diets typically contain high levels of Leu, but the other BCAA are also well above the requirement and could potentially mitigate the negative impact of Leu on BCAA catabolism. Indeed, some studies suggest that when diets contain high levels of Leu, more Ile and Val are needed to optimize growth performance. However, the precise relationship between BCAA and their balance in swine diets is not fully understood. More research is needed to understand and quantify the relationship between LNAA and BCAA.

scholarly journals 88 Dietary supplementation of branched-chain amino acids higher than recommended levels in nursery pigs fed with moderately low protein diet: effects on growth performance

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 82-82
Author(s):  
Mohammad Habibi ◽  
Cedrick N Shili ◽  
Julia Sutton ◽  
Adel Pezeshki
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Growth Performance ◽  
Feed Intake ◽  
Average Daily Gain ◽  
Branched Chain Amino Acids ◽  
Nursery Pigs ◽  
Low Protein ◽  
Branched Chain ◽  
Daily Gain

Abstract Feed cost and excess nutrients excretion are threatening the swine industry. Moderately low protein (MLP) diets are less expensive and reduce the nitrogen excretion, but they may have negative influence on growth performance of pigs. The aim of current study was to investigate the effect of MLP diets supplemented with branched-chain amino acids (BCAA; leucine, isoleucine and valine) higher than Nutrients Requirements of Swine (NRC) recommendation on the growth performance of nursery pigs. Forty-eight weaned barrows at 3 weeks of age were weighed, individually housed and randomly assigned to 6 dietary treatments including: 1) PCON: 20% crude protein (CP), 2) NCON: 14% CP; 3) C25: NCON+ limiting amino acids (LAA, i.e. lysine, methionine, threonine, and tryptophan) 25% more than NRC levels, 4) C50: NCON+LAA 50% higher than NRC recommendations; 5) CB25: NCON + (LAA+BCAA) 25% more than NRC; 6) CB50: NCON + (LAA+BCAA) 50% higher than NRC. The data were analyzed with univariate GLM with Tukey’s post-hoc test (SPSS®). The CB50 had higher final body weight, average daily gain, average daily water intake and average daily feed intake than C50 with the latter tended to be higher than NCON. Average daily feed intake, average daily gain and gain to feed ratio tended to be greater for CB25 and CB50 relative to C25 and C50, respectively. Compared to C50, CB50 improved the mean and cumulative feed intake in all the weeks. CB50 also tended to improve the body weight gain and gain: feed in week 2 relative to C50. In conclusion, supplementing MLP diets with limiting amino acid at 25% or 50% higher than NRC recommendations was failed to recover the negative effects of these diets on growth performance. Adding BCAA more than NRC levels to MLP diets improved the growth performance with the effects of added BCAA level being negligible.

scholarly journals PSIV-6 Effect of a Phytogenic Water Additive on Growth Performance, Blood Metabolites and Gene Expression of Amino Acids Transporters in Nursery Pigs Fed with Low Protein Diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 281-282
Author(s):  
Cedrick N Shili ◽  
Mohammad Habibi ◽  
Julia Sutton ◽  
Jessie Barnes ◽  
Jacob Burchkonda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Growth Performance ◽  
Mrna Abundance ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Blood Calcium ◽  
Low Protein ◽  
Protein Diets ◽  
Standard Protein

Abstract Moderately low protein (MLP) diets can help decrease nutrient excretion from the swine production. However, MLP diets negatively impact growth performance. We hypothesized that supplementing MLP diets with phytogenics may reduce the negative effects of these diets on growth. The objective of this study was to investigate the effect of a phytogenic water additive (PWA; Herbanimal®) on growth performance, blood metabolite and gene expression of amino acids transporters in pigs fed with MLP diets. Forty-eight weaned barrows were allotted to six dietary treatments (n = 8) for 4 weeks: >CON-NS: standard protein diet-no PWA; CON-LS: standard protein diet-low PWA dose (4 ml/L); CON-HS: standard protein diet-high PWA dose (8 ml/L); LP-NS: low protein diet-no PWA; LP-LS: low protein diet-low PWA dose (4 ml/L); LP-HS: low protein diet- high PWA dose (8 ml/L). Feed intake and body weight were recorded daily and weekly, respectively. At week 4, blood and tissue samples were collected and analyzed for metabolites using a chemistry analyzer and amino acid transporters using qPCR, respectively. The data were analyzed by univariate GLM (SPSS®) and the means were separated using paired Student’s t-test corrected by Benjamini-Hochberg. Pigs fed CON-HS improved the average daily gain and serum calcium and phosphorus concentrations compared to CON-NS. Pigs fed LP-LS had higher serum phosphorus and blood urea nitrogen compared to the pigs fed with LP-NS. The mRNA abundance of SLC7A11 in the jejunum was lower in CON-LS and CON-HS compared to CON-NS. Additionally, mRNA abundance of SLC6A19 in the jejunum of pigs fed with LP-LS was higher compared to LP-NS and lower in CON-HS relative to pigs fed with CON-LS. In conclusion, PWA improved the growth performance of pigs fed standard protein diets but not low protein diets. Further, the PWA improved the concentrations of blood calcium and phosphorous in pigs fed MLP diets. Funding: Agrivida and Animal Health and Production and Animal Products: Improved Nutritional Performance, Growth, and Lactation of Animals from the USDA-NIFA.

Branched-Chain Amino Acids, Nitrogen Excretion and Injury in Man

1976 ◽  
Vol 50 (5) ◽  
pp. 393-399 ◽  
Author(s):  
J. H. Wedge ◽  
R. De Campos ◽  
A. Kerr ◽  
R. Smith ◽  
Rose Farrell ◽  
...  
Keyword(s):  
Amino Acids ◽  
Venous Blood ◽  
Branched Chain Amino Acids ◽  
Nitrogen Excretion ◽  
Adult Males ◽  
Blood Concentrations ◽  
Branched Chain ◽  
Injured Patients ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

1. Venous blood concentrations of the branched-chain amino acids, valine, leucine and isoleucine, and urinary nitrogen excretion have been measured in sixteen adult males, from 2 h to 7 days after injury, and in four adults after elective skin grafts. 2. In the injured group the concentrations of these amino acids rose significantly 24 h after injury and had doubled at 4 days and remained high; in contrast the skin-graft patients showed no significant change. 3. In those injured patients with initial hyperketonaemia, defined as more than 0·2 mmol/l, the increase in concentrations of branched-chain amino acids at the fourth and seventh days after injury was significantly less than in those with normoketonaemia, and was accompanied by lower urinary nitrogen excretion throughout the whole period. 4. It is suggested that the changes in the concentration of branched-chain amino acids after injury indicate decreased uptake by muscle or excessive release due to an imbalance between protein synthesis and protein catabolism in this tissue.

scholarly journals Amino acid requirements of pigs. 3. Requirement for apparent digestible threonine of pigs in different stages of growth.

1990 ◽  
Vol 38 (3B) ◽  
pp. 609-622
Author(s):  
N.P. Lenis ◽  
J.T.M. van Diepen
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Control Diet ◽  
Maximum Growth ◽  
Positive Control ◽  
Negative Control ◽  
Net Energy ◽  
Finishing Pigs ◽  
Amino Acid Requirements ◽  
Limiting Amino Acids

Individual and group housed crossbred pigs 45 to 105 kg and 65 to 95 kg in experiments 1 and 2, respectively, were given basal diets with L-threonine 0.6, 1.2 and 1.8 g/kg. Positive and negative control diets contained total threonine 5.7 and 4.5 g/kg, respectively. To prevent other amino acids being limiting, the negative control diet was supplemented with lysine, methionine, tryptophan, isoleucine, histidine and valine. The positive control diet was supplemented with lysine and methionine. The requirement for total threonine of growing-finishing pigs for maximum growth performance was about 5.6 g/kg in a diet containing net energy 9.4 MJ/kg. This figure corresponds with about 4.7 g/kg apparent faecal digestible threonine and 4.3 apparent ileal digestible threonine. There was no difference between the growing and the finishing pigs. The requirement for ileal digestible threonine, relative to ileal digestible lysine requirement, was about 64%. It is concluded that dietary protein can be reduced by 2 percentage units without any adverse effect on growth performance, if limiting amino acids are sufficiently supplemented. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals 17 Challenges and implications of feeding diets with excess concentrations of leucine to growing-finishing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 16-16
Author(s):  
Mike D Tokach ◽  
Henrique S Cemin ◽  
Hayden R Kerkaert ◽  
Jason C Woodworth ◽  
Steve S Dritz ◽  
...  
Keyword(s):  
Amino Acids ◽  
Blood Meal ◽  
Meta Analysis ◽  
Branched Chain Amino Acids ◽  
Nitrogen Excretion ◽  
Intact Protein ◽  
Future Research ◽  
Distillers Grains With Solubles ◽  
Diet Formulation ◽  
Branched Chain

Abstract Excess dietary leucine stimulates the key enzymes involved in branched-chain catabolism causing breakdown of all branched-chain amino acids, including isoleucine and valine. Branched-chain amino acids share a common brain transporter with other large neutral amino acids (LNAA). Excess levels of one of the LNAA increases brain uptake of that amino acid and decreases the uptake of the other LNAA, including tryptophan. Thus, excess leucine can impact the requirements for many amino acids. From a practical basis, this effect was first demonstrated with diets containing blood meal, but was thought to be of limited concern unless high blood meal diets were fed. Use of corn dried distillers grains with solubles (DDGS) or high protein DDGS in corn-based diets results in diets containing excess leucine. These high leucine levels are of limited concern if adequate levels of other branched-chain amino acids and LNAA are fed, which is often the case if the diet consists largely of intact protein sources. Feed grade amino acids, such as L-lysine, L-threonine, L-tryptophan, DL-methionine, L-valine, and L-isoleucine have been widely adopted as a means to lower nitrogen excretion and diet cost. Including these amino acids in diets containing corn products reduces dietary leucine; but the resulting diets are formulated near the requirement for the first 6 limiting amino acids, including valine, isoleucine, and tryptophan, while still being high in leucine. The excess leucine increases the requirements for valine, isoleucine, and possibly other LNAA, such as tryptophan and possibly others. The exact relationship between these amino acids and how to handle them in practical diet formulation is still being elucidated; however, recent meta-analysis and experimental data confirm the importance of these ratios and provide useful direction for future research.

scholarly journals 47 Young Scholar Presentation: Meta-regression analysis to predict the influence of branched-chain and large neutral amino acids on growth performance of pigs

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 23-23
Author(s):  
Henrique S Cemin ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
...  
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Forward Selection ◽  
Negative Effects ◽  
Impact Performance ◽  
Large Neutral Amino Acids ◽  
Neutral Amino Acids ◽  
Meta Regression ◽  
Meta Regression Analysis ◽  
Branched Chain

Abstract Excess of a branched-chain amino acid (BCAA), particularly Leu, may result in increased degradation of the others due to common catabolic steps. Moreover, large neutral amino acids (LNAA) and BCAA have common brain transporters. Therefore, excessive BCAA may decrease the absorption of AA that serve as precursors of neurotransmitters. We hypothesize that practical diets with high levels of feed-grade AA can potentially create BCAA imbalances and impact performance. A meta-regression was conducted to evaluate the effects of BCAA and their interactions with LNAA to develop prediction equations for ADG, ADFI, and G:F. Data from 25 papers for a total of 44 trials were recorded in a database. Diets were reformulated using NRC (2012) nutrient values. Amino acids were expressed on standardized ileal digestible basis. A step-wise manual forward selection was used to evaluate predictor variables. Predictors had to be significant (P < 0.05) as a single variable predictor and provide a BIC improvement of at least 2 points to be included in the model. Observations were weighted using the inverse of the squared SEM. The resulting predictor equations were: ADG, g = – 985.94 + (15.2499 × average BW (kg)) – (0.08885 × average BW × average BW) + (1.063 × Leu:Lys) + (20.2659 × Ile:Lys) – (0.1479 × Ile:Lys × Ile:Lys) + (9.2243 × (Ile+Val):Leu) – (0.03321 × (Ile+Val):Leu × (Ile+Val):Leu) – (0.4413 × Ile:Trp); G:F = 648.3 – (6.2974 × average BW (kg)) + (0.02051 × average BW × average BW) + (0.5396 × Ile:Lys) + (1.7284 × Val:Lys) – (0.00795 × Val:Lys × Val:Lys) – (1.7594 × Met:Lys); and ADFI, kg = predicted ADG/predicted G:F. The equations suggest that increasing Leu:Lys negatively affects ADG due to a reduction in ADFI and G:F. The addition of Val, Ile, and Trp, alone or in combination, has the potential to counteract the negative effects of high Leu on growth performance.

Effect of residual feed intake phenotype–nutritional treatment interaction on the growth performance, plasma metabolic variables and somatotropic axis gene expression of growing ewes

2016 ◽  
Vol 56 (10) ◽  
pp. 1593 ◽  
Author(s):  
H. T. Nie ◽  
Z. Y. Wang ◽  
S. Lan ◽  
H. Zhang ◽  
Y. J. Wan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Growth Performance ◽  
Feed Intake ◽  
Mrna Abundance ◽  
Feed Restriction ◽  
Somatotropic Axis ◽  
Nutritional Treatment ◽  
Treatment Interaction ◽  
Ad Libitum

This study aimed to evaluate the effect of residual feed intake (RFI) phenotype and nutritional treatment interaction on the growth performance, plasma variables and gene expression levels within the somatotropic axis. Growing ewes [n = 52, initial bodyweight (BW) = 17.5 ± 0.5 kg, 2 months of age] were offered ad libitum access to diets for 63 days and ranked based on RFI phenotype. Thirty ewes with the highest and lowest RFI values were selected and randomly assigned to three nutritional treatments based on dry matter intake (DMI), which are ad libitum (AL), low restriction (LR) and high restriction (HR) groups, respectively. Each nutritional treatment group included ewes with high (n = 5) and low RFI (n = 5) values. During nutritional treatment (from Day 64 to Day 138), plasma samples were obtained to measure metabolite and hormone concentrations. Tissues of the hypothalamus, pituitary, liver, and Longissimus dorsi muscle (LM) were harvested at the end of the experiment (Day 138) to measure the gene expression level within the somatotropic axis. Muscle growth hormone receptor mRNA abundance of low RFI ewes tended to be greater (P = 0.09) under AL feeding, but this difference was abolished by underfeeding (P > 0.10). Low RFI ewes under HR treatment showed slightly greater growth performance, which was accompanied with lower pituitary somatostain receptor 2 mRNA abundance (P < 0.05), plasma non-esterified fatty acid concentration (P < 0.05), and greater concentration of triglyceride (P < 0.05), compared with ewes classified as high RFI group. Our results suggested that ewes categorised as low RFI showed higher resistance to the condition of high feed restriction, which might be attributed to less intensity of fat mobilisation under negative energy balance. The mechanism underlying resistance to such feed restriction was presumably through action of somatostain receptor 2 and was potentially mediated by inhibitory effects of somatostatin on growth hormone release but not basal growth hormone secretion.

scholarly journals Branched-Chain Amino Acids Are the Primary Limiting Amino Acids in the Diets of Endurance-Trained Men after a Bout of Prolonged Exercise

2018 ◽  
Vol 148 (6) ◽  
pp. 925-931 ◽  
Author(s):  
Hiroyuki Kato ◽  
Katsuya Suzuki ◽  
Makoto Bannai ◽  
Daniel R Moore
Keyword(s):  
Amino Acids ◽  
Prolonged Exercise ◽  
Branched Chain Amino Acids ◽  
Branched Chain ◽  
Limiting Amino Acids
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