scholarly journals PSII-5 Chemical composition, in vitro dry matter digestibility, gas production and methane emission of maintenance, growth and production diets/rations of buffaloes

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 408-408
Author(s):  
Uchenna Anele ◽  
Sultan Singh ◽  
B P Kushwaha ◽  
P K Gupta ◽  
S Bhattacharya
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Dry Matter ◽  
Chemical Constituents ◽  
Gas Production ◽  
Protein Fractions ◽  
Mean Values ◽  
Dry Matter Digestibility ◽  
Structural Carbohydrate

Abstract Ten types of diets were prepared to meet the nutrient requirements of buffaloes for maintenance (MD1 to MD10), growth (GD1 to GD10) and production/lactation (PD1 to PD10). A total of 30 diets were evaluated for chemical composition, in vitro gas and methane production, and dry matter (DM) disappearance using buffalo rumen liquor. The CNCPS was used to estimate the carbohydrate and protein fractions. Protein fractions PB1 and PB2 of maintenance diets were lower (P < 0.05) than growth and production diets, while protein fractions (PB3) and Pc were (P < 0.05) higher in maintenance than in growth and production diets. Mean values of PA was (P < 0.05) higher in growth diets (136.9) than production (114.8) and maintenance diets (105.6 g/kg DM). Maintenance diets had (P < 0.05) higher structural carbohydrate contents (586.2 g/kg DM) than production diets (513.0 g/kg DM). Carbohydrate fraction (CB1) was highest (P < 0.05) in production diets (187.2 g/kg DM) followed by growth (129.5 g/kg DM) and maintenance diets (96.1 g/kg DM). In vitro gas production at different time periods (12, 24 and 48 h) was similar for maintenance (63.04, 51.98 and 48.15 ml/g DM), growth (63.83, 52.73 and 48.250) and production diets (63.51, 52.54 and 47.21 ml/g DM). Cumulative methane production was numerically lower for maintenance (28.40 ml/g DM) than growth (29.58 ml/g DM) and production diets (33.13 ml/g DM). In vitro methane production as a proportion of degraded DM (ml/g DDM and g/kg DDM) was similar for maintenance (14.21 and 29.53), growth (42.19 and 30.25) and production diets (41.26 and 29.58). Dietary chemical constituents such as EE, lignin, NDIN, ADIN and PB3 and Cc were (P < 0.05) negatively associated with methane production, while OM, NPN, SP, PA and PB1, TCHO and CB2 were positively (P < 0.05) correlated with methane production.

scholarly journals Influence of Kappaphycus Alvarezii and Gracilaria Salicornia Supplementation on in Vitro Fermentation Pattern, Total Gas and Methane Production of Mixed Substrates

2021 ◽  
Author(s):  
Vithal K. Munde ◽  
Asit Das ◽  
Putan Singh ◽  
Ashok K. Verma ◽  
Nirmala Muwel ◽  
...  
Keyword(s):  
Rate Constant ◽  
Methane Production ◽  
Dry Matter ◽  
Kappaphycus Alvarezii ◽  
Gas Production ◽  
Mixed Substrates ◽  
Gracilaria Salicornia ◽  
Dry Matter Digestibility ◽  
Fermentation Pattern

Abstract This experiment was conducted to study the effect of supplementation of Kappaphycus alvarezii (KA) and Gracilaria salicornia (GS) in vitro fermentation pattern, total gas and methane production of mixed substrates. Basal substrate comprising of concentrates and wheat straw (50:50) was supplemented with either 0% (control), 1 (KA1), 2 (KA2), 4 (KA4), 6 (KA6), and 8 % (KA8) of Kappaphycus; and, 1 (GS1), 2 (GS2), 4 (GS4), 6 (GS6), and 8 (GS8 ) of Gracilaria, respectively. Asymptote, rate constant of gas production and t-half, concentration of total volatile fatty acids (TVFA), and in vitro dry matter digestibility (IVDMD) was not affected up to 2% level KA supplementation, beyond which asymptote, and rate constant of gas production, TVFA, and IVDMD decreased and t-half increased (P<0.001). Asymptote, rate constant of gas production, TVFA and IVDMD was not affected at 1% level of inclusion, beyond which a steady decline in these parameters was observed (P<0.001). Methane production (ml/g DM) was higher (P<0.001) in CON, followed by KA1 and KA2, and lower values were observed in by KA4, KA6 and KA8. Methane production (ml/kg DM) declined (P<0.001) steadily with increased level of GS in the substrates. From the results it was concluded that inclusion of Kappaphycus alvarezii and Gracilaria salicornia at 2 and 1%, respectively in the fermentation substrate can reduce in vitro methane production without any adverse impact on total gas production and in vitro dry matter digestibility.

Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Application Rates ◽  
The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique

2013 ◽  
Vol 93 (3) ◽  
pp. 399-402
Author(s):  
J. C. Plaizier ◽  
S. Li
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Short Communication ◽  
Prediction Equation ◽  
Gas Production ◽  
Production Technique ◽  
Dry Matter Digestibility ◽  
Grain Crop

Plaizier, J. C. and Li, S. 2013. Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique. Can. J. Anim. Sci. 93: 399–402. The potential of the gas production technique to predict in vitro dry matter digestibility was determined using 50 ruminant feeds, including grass/alfalfa forages, grain crop silages, grains, high protein feeds, and feed straws. Without including the chemical composition in the prediction equation, the prediction of IVDMD based on the cumulative gas production during 48 h alone was poor (R 2=0.48). Including the crude protein and neutral detergent fibre concentrations of the feed in the prediction equation improved the accuracy of the prediction (R 2=0.87), which was higher than that of predicting IVDMD based on the chemical composition alone (R 2=0.82).

scholarly journals Silage of Intercropping Corn, Palisade Grass, and Pigeon Pea Increases Protein Content and Reduces In Vitro Methane Production

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1784
Author(s):  
Beatriz Ligoski ◽  
Lucas Ferreira Gonçalves ◽  
Flavio Lopes Claudio ◽  
Estenio Moreira Alves ◽  
Ana Maria Krüger ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Nutritional Quality ◽  
Production Systems ◽  
Animal Feed ◽  
Lignin Content ◽  
Pigeon Pea ◽  
Gas Production ◽  
In Vitro Gas Production

Legume–grass intercropping systems are a sustainable option to improve nutritional quality of animal feed and decrease livestock greenhouse gas emissions. Thus, the present study evaluated yield, chemical composition and in vitro gas production of silages produced with intercropped palisade grass (Urochloa brizantha.(A.Rich.) R.D.Webster), pigeon pea (Cajanus cajan cv. Super N) and corn (Zea mays. L.). Forage was harvested and placed inside micro-silos, which were opened after 100 days and samples were collected for chemical composition and in vitro gas production analyses. Intercropped silage had higher crude protein, acid detergent fiber, and lignin content than corn silage. Moreover, intercropped silage decreased total gas and methane production. Therefore, intercropped silage showed potential to increase conserved feed nutritional quality and reduce methane emissions in livestock production systems.

scholarly journals Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Positive Control ◽  
Rumen Fermentation ◽  
Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

Microbial profiles, in vitro gas production and dry matter digestibility based on various ratios of roughage to concentrate

2012 ◽  
Vol 63 (2) ◽  
pp. 541-545 ◽  
Author(s):  
Sanjay Kumar ◽  
Sumit Singh Dagar ◽  
Sunil Kumar Sirohi ◽  
Ramesh Chandra Upadhyay ◽  
Anil Kumar Puniya
Keyword(s):  
Dry Matter ◽  
Gas Production ◽  
Dry Matter Digestibility ◽  
In Vitro Gas Production

In vitromicrobial growth and rumen fermentation of different substrates as affected by the addition of disodium malate

2005 ◽  
Vol 81 (1) ◽  
pp. 31-38 ◽  
Author(s):  
M. L. Tejido ◽  
M. J. Ranilla ◽  
R. García-Martínez ◽  
M. D. Carro
Keyword(s):  
Dry Matter ◽  
Microbial Growth ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Mean Values ◽  
Batch Cultures ◽  
Production Values ◽  
Micro Organisms ◽  
Microbial N

AbstractThe effects of two concentrations of disodium malate on thein vitrofermentation of three substrates differing in their forage: concentrate ratio (0·8: 0·2, 0·5: 0·5 and 0·2: 0·8; g/g dry matter; low-, medium- and high-concentrate substrates, respectively) by rumen micro-organisms were studied using batch cultures. Rumen contents were collected from four Merino sheep offered lucerne hay ad libitum and supplemented daily with 400 g concentrate. Disodium malate was added to the incubation bottles to achieve final concentrations of 0, 4 and 8 mmol/l malate and15N was used as a microbial marker. Gas production was measured at regular intervals from 0 to 120 h of incubation to study fermentation kinetics. When gas production values were corrected for gas released from added malate, no effects (P> 0·05) of malate were detected for any of the estimated gas production parameters. In 17-h incubations, the final pH and total volatile fatty acid (VFA) production were increased (P< 0·001) by the addition of malate, but no changes (P> 0·05) were detected in the final amounts of ammonia-N and lactate. When net VFA productions were corrected for the amount of VFA produced from malate fermentation itself, adding malate did not affect (P> 0·05) the production of acetate, propionate and total VFA. Malate reduced methane (CH4) production by proportionately 0·058, 0·013 and 0·054 for the low-, medium- and high-concentrate substrates, respectively. Adding malate to batch cultures increased (P< 0·01) rumen microbial growth (mean values of 16·6, 18·3 and 18·4 mg of microbial N for malate at 0, 4 and 8 mmol/l, respectively), but did not affect (P> 0·05) its efficiency of growth (55·5, 56·7 and 54·3 mg of microbial N per g of organic matter apparently fermented for malate at 0, 4 and 8 mmol/l, respectively). There were no interactions (P> 0·05) malate × substrate for any of the measured variables, and no differences (P> 0·05) in pH, CH4production and microbial growth were found between malate at 4 and 8 mmol/l. The results indicate that malate had a beneficial effect on in vitro rumen fermentation of substrates by increasing VFA production and microbial growth, and that only subtle differences in the effects of malate were observed between substrates. Most of the observed effects, however, seem to be due to fermentation of malate itself.

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