scholarly journals Isoflurane in Contrast to Propofol Promotes Fluid Extravasation during Cardiopulmonary Bypass in Pigs

2013 ◽  
Vol 119 (4) ◽  
pp. 861-870 ◽  
Author(s):  
Hege Kristin Brekke ◽  
Stig Morten Hammersborg ◽  
Steinar Lundemoen ◽  
Arve Mongstad ◽  
Venny Lise Kvalheim ◽  
...  
Keyword(s):  
Cardiopulmonary Bypass ◽  
Water Content ◽  
Interstitial Fluid ◽  
Outcome Analysis ◽  
Tissue Water Content ◽  
Tissue Water ◽  
Edema Formation ◽  
Fluid Extravasation ◽  
Isoflurane Anesthesia ◽  
Total Tissue

Abstract Background: A highly positive intraoperative fluid balance should be prevented as it negatively impacts patient outcome. Analysis of volume-kinetics has identified an increase in interstitial fluid volume after crystalloid fluid loading during isoflurane anesthesia. Isoflurane has also been associated with postoperative hypoxemia and may be associated with an increase in alveolar epithelial permeability, edema formation, and hindered oxygen exchange. In this article, the authors compare fluid extravasation rates before and during cardiopulmonary bypass (CPB) with isoflurane- versus propofol-based anesthesia. Methods: Fourteen pigs underwent 2 h of tepid CPB with propofol (P-group; n = 7) or isoflurane anesthesia (I-group; n = 7). Fluid requirements, plasma volume, colloid osmotic pressures in plasma and interstitial fluid, hematocrit levels, and total tissue water content were recorded, and fluid extravasation rates calculated. Results: Fluid extravasation rates increased in the I-group from the pre-CPB level of 0.27 (0.13) to 0.92 (0.36) ml·kg−1·min−1, but remained essentially unchanged in the P-group with significant between-group differences during CPB (pb = 0.002). The results are supported by corresponding changes in interstitial colloid osmotic pressure and total tissue water content. Conclusions: During CPB, isoflurane, in contrast to propofol, significantly contributes to a general increase in fluid shifts from the intravascular to the interstitial space with edema formation and a possible negative impact on postoperative organ function.

Intraoperative fluid balance during cardiopulmonary bypass: effects of different mean arterial pressures

Perfusion ◽  
2007 ◽  
Vol 22 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Oddbjørn Haugen ◽  
Marit Farstad ◽  
Venny Kvalheim ◽  
Stig Hammersborg ◽  
Paul Husby
Keyword(s):  
Cardiopulmonary Bypass ◽  
Plasma Volume ◽  
Fluid Balance ◽  
Financial Support ◽  
Blood Chemistry ◽  
Tissue Water Content ◽  
Regional Health ◽  
Tissue Water ◽  
Fluid Extravasation ◽  
Fluid Shifts

Financial support . This study was financially supported by The Western Norway Regional Health Authority, The Norwegian Council on Cardiovascular Diseases, Faculty of Medicine, University of Bergen and The Frank Mohn Foundation, Norway. Introduction. This study investigated whether two levels of mean arterial pressure (MAP) during cardiopulmonary bypass did influence per-operative fluid shifts. Methods. Sixteen pigs underwent 60 minutes of normothermic cardiopulmonary bypass (CPB) followed by 90 minutes of hypothermic CPB. Eight animals had a MAP of 60—80 mmHg by norepinephrine (HP group). Another 8 animals had a MAP of 40—45 mmHg by phentolamine (LP group). Blood chemistry, plasma/interstitial colloid osmotic pressures, plasma volume, fluid balance, fluid extravasation rate and tissue water content were measured or calculated. Results. The plasma volume was significantly lower in the HP group compared with the LP group after 60 minutes of CPB. Net fluid balance was 0.18 (0.05) ml·kg-1·min -1 in the HP group and 0.21 ml·kg- 1·min-1 in the LP group (P > 0.05) while fluid extravasation rate was 1.18 (0.5) and 1.13 (0.4) ml·kg -1·min-1 in the HP group and the LP group during CPB (P > 0.05). Conclusion. Net fluid balance and fluid extravasation rate were similar in the animals with elevated and with lowered MAP during CPB. Perfusion (2007) 22, 273—278.

Detection of bark lesions caused by Phytophthoracinnamomi in Eucalyptusmarginata with the plant impedance ratio meter and the Shigometer

1987 ◽  
Vol 17 (10) ◽  
pp. 1228-1233 ◽  
Author(s):  
Joanna T. Tippett ◽  
Joanne L. Barclay
Keyword(s):  
Water Content ◽  
Electrical Resistance ◽  
Water Status ◽  
Healthy Tissue ◽  
Plant Tissues ◽  
Tissue Water Content ◽  
Tissue Water ◽  
Impedance Ratio ◽  
New Instrument ◽  
Electrical Admittance

A new instrument, the plant impedance ratio meter (PIRM), has been used to determine the extent of lesions caused by Phytophthoracinnamomi Rands in Eucalyptusmarginata Sm. The performance of the PIRM, which measures electrical admittance (the inverse of impedance) of plant tissues at two frequencies (from which an impedance ratio is calculated), was evaluated and compared with that of the Shigometer. The electrical admittance measured at 1 and 10 kHz (PIRM) and the electrical resistance (Shigometer) of healthy tissue varied with both the depth of tissue probed and the water status of stems. However, the impedance ratios (calculated from the admittance values) remained relatively constant for healthy tissue and changes were independent of depth of probing. Hence, changes in ratios indicated a change in tissue condition or necrosis rather than changes in either tissue water content or depth of probing. The impedance ratios recorded for healthy bark tissues were consistently higher than those for the P. cinnamomi lesions in E. marginata. Trends in electrical resistance measured across the boundaries of the lesions with the Shigometer were variable depending on lesion age. The PIRM was used successfully to detect P. cinnamomi lesions in E. marinate and lesion fronts were predicted to an accuracy of ± 7.2 mm (n = 150), lesions being up to 1.0 m long at the time stems were harvested.

Microvascular fluid exchange during CPB with deep hypothermia circulatory arrest or low flow

Perfusion ◽  
2017 ◽  
Vol 32 (8) ◽  
pp. 661-669
Author(s):  
Bjørg Elvevoll ◽  
Paul Husby ◽  
Venny L. Kvalheim ◽  
Lodve Stangeland ◽  
Arve Mongstad ◽  
...  
Keyword(s):  
Brain Injury ◽  
Circulatory Arrest ◽  
Deep Hypothermia ◽  
Low Flow ◽  
Cerebral Microdialysis ◽  
Tissue Water ◽  
Fluid Exchange ◽  
Fluid Extravasation ◽  
Total Tissue ◽  
Water Contents

Objective: Use of deep hypothermic low-flow (DHLF) cardiopulmonary bypass (CPB) has been associated with higher fluid loading than the use of deep hypothermia circulatory arrest (DHCA). We evaluated whether these perfusion strategies influenced fluid extravasation rates and edema generation differently per-operatively. Materials and Methods: Twelve anesthetized pigs, randomly allocated to DHLF (n = 6) or DHCA (n = 6), underwent 2.5 hours CPB with cooling to 20°C for 30 minutes (min), followed by 30 min arrested circulation (DHCA) or 30 min low-flow circulation (DHLF) before 90 min rewarming to normothermia. Perfusion of tissues, fluid requirements, plasma volumes, colloid osmotic pressures and total tissue water contents were recorded and fluid extravasation rates calculated. During the experiments, cerebral microdialysis was performed in both groups. Results: Microvascular fluid homeostasis was similar in both groups, with no between-group differences, reflected by similar fluid extravasation rates, plasma colloid osmotic pressures and total tissue water contents. Although extravasation rates increased dramatically from 0.10 (0.11) ml/kg/min (mean with standard deviation in parentheses) and 0.16 (0.02) ml/kg/min to 1.28 (0.58) ml/kg/min and 1.06 (0.41) ml/kg/min (DHCA and DHLF, respectively) after the initiation of CPB, fluid filtrations during both cardiac arrest and low flow were modest and close to baseline values. Cerebral microdialysis indicated anaerobic metabolism and ischemic brain injury in the DHCA group. Conclusion: No differences in microvascular fluid exchange could be demonstrated as a direct effect of DHCA compared with DHLF. Thirty minutes of DHCA was associated with anaerobic cerebral metabolism and possible brain injury.

scholarly journals Algorithm for Mapping Kidney Tissue Water Content during Normothermic Machine Perfusion Using Hyperspectral Imaging

Algorithms ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 289
Author(s):  
Wenke Markgraf ◽  
Jannis Lilienthal ◽  
Philipp Feistel ◽  
Christine Thiele ◽  
Hagen Malberg
Keyword(s):  
Water Content ◽  
Hyperspectral Imaging ◽  
Wavelength Range ◽  
Kidney Tissue ◽  
Reflectance Spectra ◽  
Machine Perfusion ◽  
Least Squares Regression ◽  
Tissue Water Content ◽  
Tissue Water ◽  
Normothermic Machine Perfusion

The preservation of kidneys using normothermic machine perfusion (NMP) prior to transplantation has the potential for predictive evaluation of organ quality. Investigations concerning the quantitative assessment of physiological tissue parameters and their dependence on organ function lack in this context. In this study, hyperspectral imaging (HSI) in the wavelength range of 500–995 nm was conducted for the determination of tissue water content (TWC) in kidneys. The quantitative relationship between spectral data and the reference TWC values was established by partial least squares regression (PLSR). Different preprocessing methods were applied to investigate their influence on predicting the TWC of kidneys. In the full wavelength range, the best models for absorbance and reflectance spectra provided Rp2 values of 0.968 and 0.963, as well as root-mean-square error of prediction (RMSEP) values of 2.016 and 2.155, respectively. Considering an optimal wavelength range (800–980 nm), the best model based on reflectance spectra (Rp2 value of 0.941, RMSEP value of 3.202). Finally, the visualization of TWC distribution in all pixels of kidneys’ HSI image was implemented. The results show the feasibility of HSI for a non-invasively and accurate TWC prediction in kidneys, which could be used in the future to assess the quality of kidneys during the preservation period.

USE OF CONTROLLED ENVIRONMENTS FOR WINTER CEREAL COLD HARDINESS EVALUATION: CONTROLLED FREEZE TESTS AND TISSUE WATER CONTENT AS PREDICTION TESTS

1989 ◽  
Vol 69 (2) ◽  
pp. 355-366 ◽  
Author(s):  
A. L. BRULE-BABEL ◽  
D. B. FOWLER
Keyword(s):  
Water Content ◽  
Cold Acclimation ◽  
Cold Hardiness ◽  
Screening Method ◽  
Strong Relationship ◽  
Winter Rye ◽  
Tissue Water Content ◽  
Tissue Water ◽  
Winter Cereal ◽  
Controlled Environments

Field survival is the most commonly employed method of evaluating the winter hardiness of cereals. However, the inherent difficulties with field trials have stimulated a continued interest in the use of controlled environments and prediction tests for the evaluation of cold hardiness. In the present studies, cold hardiness expression of wheat (Triticum aestivum L.) cultivars acclimated in controlled environments was found to be similar to that reported for field conditions in Saskatchewan, Canada. LT50 and tissue water content measurements on wheat and rye (Secale cereale L.) cultivars acclimated in controlled environments were highly correlated with cultivar field survival ability. Investigation of the relationship between field survival and tissue water content during cold acclimation in controlled environments indicated that, to be effective as a screening method for cold hardiness, measurements of tissue water content should be made on fully acclimated plants for which the acclimation conditions have been rigorously controlled. Level of acclimation was not as critical for cold hardiness screening when LT50 measurements were utilized; however, maximum resolution also required fully acclimated plants. Although a strong relationship (r = −0.80 to −0.89) was found to exist with field survival potential, an inability to detect small, but important, differences without excessive replication would generally restrict the use of LT50 and tissue water content to situations where large homogeneous plant populations were available and only coarse screens for cold hardiness were required.Key words: Cold acclimation, winter wheat, winter rye, cold hardiness, water content, replication

Responses of the moss Tortula ruralis to desiccation treatments. II. Variations in desiccation tolerance

1981 ◽  
Vol 59 (12) ◽  
pp. 2707-2712 ◽  
Author(s):  
Mark W. Schonbeck ◽  
J. Derek Bewley
Keyword(s):  
Water Content ◽  
Desiccation Tolerance ◽  
Dry Weight ◽  
Severe Damage ◽  
Tissue Water Content ◽  
Tissue Water ◽  
Tortula Ruralis

Variations in the apparent desiccation tolerance of the moss Tortula ruralis were traced to several causes. When the moss was kept continuously hydrated for several days, desiccation tolerance tended to decrease ("dehardening"). Conversely, daily episodes of drying and rehydration induced hardening so that the moss could tolerate rapid drying to a tissue water content of 0.008 g∙g dry weight−1.Different clumps of moss showed very different degrees of desiccation tolerance. The least tolerant samples suffered severe damage after rapid drying, whereas the most tolerant suffered little damage, and did not deharden during 3 days continuous hydration.

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