scholarly journals Genetic variability among group A and B respiratory syncytial viruses in Mozambique: identification of a new cluster of group B isolates

2001 ◽  
Vol 82 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Anna Roca ◽  
Mari-Paz Loscertales ◽  
Llorenç Quintó ◽  
Pilar Pérez-Breña ◽  
Neide Vaz ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Protein Gene ◽  
N Protein ◽  
Glycosylation Sites ◽  
Rsv Infection ◽  
Group A ◽  
Respiratory Syncytial Viruses ◽  
Syncytial Virus ◽  
Group B ◽  
Vulnerable Adults

Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection in children and vulnerable adults, but little is known regarding RSV infection in Africa. In this report, a recent RSV outbreak in Mozambique was studied and results showed that 275 of 3192 (8·6%) nasopharyngeal aspirates tested were RSV-positive by ELISA. RSV presents two antigenic groups (A and B) with a high genetic and antigenic variability between and within them. Analysis by a new RFLP assay of RT–PCR amplified N protein gene products showed a higher prevalence of group B RSV than that of group A (85% versus 15%). However, genetic variability of the G protein gene was higher among group A RSV strains. The frequency and pattern of glycosylation sites were also quite different between both groups. In addition, two different phylogenetic clusters of Mozambican viruses were found within each group, but only sequences from cluster B-I were relatively distinct from previously described isolates. The implications of such differences in the antigenic and immunogenic characteristics of each group are discussed.

scholarly journals Genetic Variability in the G Protein Gene of Group A and B Respiratory Syncytial Viruses from India

2006 ◽  
Vol 44 (9) ◽  
pp. 3055-3064 ◽  
Author(s):  
S. Parveen ◽  
W. M. Sullender ◽  
K. Fowler ◽  
E. J. Lefkowitz ◽  
S. K. Kapoor ◽  
...  
Keyword(s):  
Genetic Variability ◽  
G Protein ◽  
Protein Gene ◽  
Group A ◽  
Respiratory Syncytial Viruses

scholarly journals Molecular epidemiology of respiratory syncytial virus in The Gambia

1999 ◽  
Vol 122 (1) ◽  
pp. 155-160 ◽  
Author(s):  
P. A. CANE ◽  
M. WEBER ◽  
M. SANNEH ◽  
R. DACKOUR ◽  
C. R. PRINGLE ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Genetic Variability ◽  
G Protein ◽  
Protein Gene ◽  
The Gambia ◽  
The World ◽  
Rsv Infection ◽  
Syncytial Virus ◽  
The Uk ◽  
Developed World

Respiratory syncytial virus (RSV) infection in The Gambia occurs seasonally in association with the rainy season. This study examined the genetic variability of RSV isolates from four consecutive epidemics from 1993–6. Each epidemic was made up of a number of variants which were replaced in subsequent epidemics. Analysis of attachment (G) protein gene sequences showed that isolates were closely related to those observed in the rest of the world. However, many isolates from 1993 and 1994 were unlike other isolates observed in the developed world during this period and were more similar to isolates from 1984 in Europe. In addition, the most commonly observed genotype in the UK in the 1990s was not detected in The Gambia during this period.

scholarly journals Clinical patterns and seasonal trends in respiratory syncytial virus hospitalizations in São Paulo, Brazil

2001 ◽  
Vol 43 (3) ◽  
pp. 125-131 ◽  
Author(s):  
Sandra E. VIEIRA ◽  
Klaus E. STEWIEN ◽  
Divina A. O. QUEIROZ ◽  
Edison L. DURIGON ◽  
Thomas J. TÖRÖK ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Lower Respiratory Tract ◽  
Respiratory Diseases ◽  
Respiratory Viruses ◽  
Hospitalized Children ◽  
Seasonal Trends ◽  
Group A ◽  
Clinical Patterns ◽  
Syncytial Virus ◽  
Group B

The respiratory viruses are recognized as the most frequent lower respiratory tract pathogens for infants and young children in developed countries but less is known for developing populations. The authors conducted a prospective study to evaluate the occurrence, clinical patterns, and seasonal trends of viral infections among hospitalized children with lower respiratory tract disease (Group A). The presence of respiratory viruses in children's nasopharyngeal was assessed at admission in a pediatric ward. Cell cultures and immunofluorescence assays were used for viral identification. Complementary tests included blood and pleural cultures conducted for bacterial investigation. Clinical data and radiological exams were recorded at admission and throughout the hospitalization period. To better evaluate the results, a non- respiratory group of patients (Group B) was also constituted for comparison. Starting in February 1995, during a period of 18 months, 414 children were included- 239 in Group A and 175 in Group B. In Group A, 111 children (46.4%) had 114 viruses detected while only 5 children (2.9%) presented viruses in Group B. Respiratory Syncytial Virus was detected in 100 children from Group A (41.8%), Adenovirus in 11 (4.6%), Influenza A virus in 2 (0.8%), and Parainfluenza virus in one child (0.4%). In Group A, aerobic bacteria were found in 14 cases (5.8%). Respiratory Syncytial Virus was associated to other viruses and/or bacteria in six cases. There were two seasonal trends for Respiratory Syncytial Virus cases, which peaked in May and June. All children affected by the virus were younger than 3 years of age, mostly less than one year old. Episodic diffuse bronchial commitment and/or focal alveolar condensation were the clinical patterns more often associated to Respiratory Syncytial Virus cases. All children from Group A survived. In conclusion, it was observed that Respiratory Syncytial Virus was the most frequent pathogen found in hospitalized children admitted for severe respiratory diseases. Affected children were predominantly infants and boys presenting bronchiolitis and focal pneumonias. Similarly to what occurs in other subtropical regions, the virus outbreaks peak in the fall and their occurrence extends to the winter, which parallels an increase in hospital admissions due to respiratory diseases.

scholarly journals Dominance of the ON1 Genotype of RSV-A and BA9 Genotype of RSV-B in Respiratory Cases from Jeddah, Saudi Arabia

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1323
Author(s):  
Hessa A. Al-Sharif ◽  
Sherif A. El-Kafrawy ◽  
Jehad M. Yousef ◽  
Taha A. Kumosani ◽  
Mohammad A. Kamal ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Respiratory Infections ◽  
Hypervariable Region ◽  
Genotype Distribution ◽  
Potential Development ◽  
The Usa ◽  
Group A ◽  
Syncytial Virus ◽  
Group B

Human respiratory syncytial virus (HRSV) is a main cause of hospital admission for lower respiratory tract infection. In previous studies from Saudi Arabia, higher prevalence of the NA1 genotype in group A was observed from Riyadh and Taif. This study recruited respiratory cases from Jeddah during January to December, 2017. RSV represented 13.4% in the recruited cases with 64% of them belonging to group A and 36% to group B. All group A cases in this study were ON1 type characterized by duplication of 72 nucleotides, 24 amino acids in the C-terminal in the second hypervariable region of the G gene. In addition, for group B all of the cases were clustered under BA9, which had uniquely characterized as duplication of 60 nucleotides in the G protein. Our sequences showed similarity with earlier sequences from Saudi Arabia, Kuwait, Thailand, South Africa, Spain, the USA and Cyprus. Some amino acid substitutions in the investigated sequences would cause a change in potential O-glycosylation and N-glycosylation profiles from prototype ON1. The predominance of the ON1 and BA9 genotype of RSV-A in Jeddah compared to previous Saudi studies showing predominance of the NA1 genotype for group A. This difference in genotype prevalence could be due to fast spread of the ON1 genotype worldwide or due to the flux of travelers through Jeddah during hajj/umrah compared to Riyadh and Taif. This shift in genotype distribution requires continuous surveillance for genetic characterization of circulating respiratory infections including RSV. These findings may contribute to the understanding of RSV evolution and to the potential development of a vaccine against RSV.

scholarly journals Evolution of respiratory syncytial virus genotype BA in Kilifi, Kenya, 15 years on

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Everlyn Kamau ◽  
James R. Otieno ◽  
Clement S. Lewa ◽  
Anthony Mwema ◽  
Nickson Murunga ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Population ◽  
Seasonal Fluctuations ◽  
Surveillance Period ◽  
Virus Genotype ◽  
Discrete Location ◽  
Glycoprotein Gene ◽  
Syncytial Virus ◽  
Group B ◽  
Vulnerable Adults

AbstractRespiratory syncytial virus (RSV) is recognised as a leading cause of severe acute respiratory disease and deaths among infants and vulnerable adults. Clinical RSV isolates can be divided into several known genotypes. RSV genotype BA, characterised by a 60-nucleotide duplication in the G glycoprotein gene, emerged in 1999 and quickly disseminated globally replacing other RSV group B genotypes. Continual molecular epidemiology is critical to understand the evolutionary processes maintaining the success of the BA viruses. We analysed 735 G gene sequences from samples collected from paediatric patients in Kilifi, Kenya, between 2003 and 2017. The virus population comprised of several genetically distinct variants (n = 56) co-circulating within and between epidemics. In addition, there was consistent seasonal fluctuations in relative genetic diversity. Amino acid changes increasingly accumulated over the surveillance period including two residues (N178S and Q180R) that mapped to monoclonal antibody 2D10 epitopes, as well as addition of putative N-glycosylation sequons. Further, switching and toggling of amino acids within and between epidemics was observed. On a global phylogeny, the BA viruses from different countries form geographically isolated clusters suggesting substantial localized variants. This study offers insights into longitudinal population dynamics of a globally endemic RSV genotype within a discrete location.

Genetic variability in envelope-associated protein genes of closely related group A strains of respiratory syncytial virus

1999 ◽  
Vol 59 (1) ◽  
pp. 89-99 ◽  
Author(s):  
HaoQiang Zheng ◽  
Greg A. Storch ◽  
Chunyi Zang ◽  
Teresa C.T. Peret ◽  
Chung S. Park ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Genetic Variability ◽  
Group A ◽  
Related Group ◽  
Syncytial Virus

Antigenic and genetic variability of human respiratory syncytial viruses (group A) isolated in Uruguay and Argentina: 1993-2001

2003 ◽  
Vol 71 (2) ◽  
pp. 305-312 ◽  
Author(s):  
Sandra Frabasile ◽  
Adriana Delfraro ◽  
Luj�n Facal ◽  
Cristina Videla ◽  
M�nica Galiano ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Group A ◽  
Respiratory Syncytial Viruses

scholarly journals 1409. Genomic Variation Among Respiratory Syncytial Viruses

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S712-S712
Author(s):  
Christopher S Anderson ◽  
Yun Zhang ◽  
Anthony Corbett ◽  
Chin-Yi Chu ◽  
Lu Wang ◽  
...  
Keyword(s):  
Protein Structure ◽  
Disease Severity ◽  
Genomic Variation ◽  
Structure Variation ◽  
Time Period ◽  
Nasal Swabs ◽  
Rsv Infection ◽  
Respiratory Syncytial Viruses ◽  
Syncytial Virus ◽  
Over Time

Abstract Background Respiratory Syncytial Virus (RSV) can be easily classified into two subtypes (A and B) based on the nucleic acid sequence of their genome. Phylogenic approaches have shown that within both subtypes separate lineages of viruses exist and new lineages continue to emerge. The role these genomic variations play in disease severity during RSV infection is largely unknown. Methods Next-generation viral RNA sequencing was performed on archived frozen nasal swabs of children infected with RSV in Rochester, NY between 1977-1998. Genomic variation was compared across year-of-isolation, age of host, and inpatient/outpatient status of host. Local RSV genomic variation was compared to variation of publicly available sequences isolated from hosts residing in other parts of the world. Results A and B subtypes demonstrated significant differences in the genetic sequence and primary-protein structure over time. G-protein was the most variable in both subtypes, but they differed in the number of unique genotypes detected. We found a significant association with disease severity (inpatient/outpatient status) and RSV phylogenetic topology, although the magnitude of the association differed by subtype. Variation in the primary protein structure of RSV viral proteins was also significantly associated with disease severity, but depended on which viral protein, and which subtype, was investigated. Lastly, local RSV genomic and protein-structure variation was similar to what was seen globally during this time period. Conclusion Overall, both subtypes demonstrated significant genetic change over time and these changes were associated with disease severity. These results suggest that the genetic variability of RSV may affect RSV disease in humans. Disclosures All Authors: No reported disclosures

Sign in / Sign up

Export Citation Format

Share Document