Modular DC Test System

2019 ◽  
Author(s):  
T. Steiner ◽  
R. Pietsch ◽  
U. Kaltenborn ◽  
M. Hensel
Keyword(s):  
Test System

Immunogold labeling of CMP-KDO synthetase produced by recombinant E. Coli cells

1987 ◽  
Vol 45 ◽  
pp. 924-925
Author(s):  
F. A. Durum ◽  
R. G. Goldman ◽  
T. J. Bolling ◽  
M. F. Miller
Keyword(s):  
Inclusion Bodies ◽  
Large Scale ◽  
Recombinant Dna ◽  
Test System ◽  
Cell Protein ◽  
Gram Negative Bacteria ◽  
Cytoplasmic Inclusions ◽  
Isolation And Purification ◽  
E Coli ◽  
Low Concentrations

CMP-KDO synthetase (CKS) is an enzyme which plays a key role in the synthesis of LPS, an outer membrane component unique to gram negative bacteria. CKS activates KDO to CMP-KDO for incorporation into LPS. The enzyme is normally present in low concentrations (0.02% of total cell protein) which makes it difficult to perform large scale isolation and purification. Recently, the gene for CKS from E. coli was cloned and various recombinant DNA constructs overproducing CKS several thousandfold (unpublished data) were derived. Interestingly, no cytoplasmic inclusions of overproduced CKS were observed by EM (Fig. 1) which is in contrast to other reports of large proteinaceous inclusion bodies in various overproducing recombinant strains. The present immunocytochemical study was undertaken to localize CKS in these cells.Immune labeling conditions were first optimized using a previously described cell-free test system. Briefly, this involves soaking small blocks of polymerized bovine serum albumin in purified CKS antigen and subjecting them to various fixation, embedding and immunochemical conditions.

Real Ear Sound Pressure Levels Developed by Three Portable Stereo System Earphones

1992 ◽  
Vol 1 (4) ◽  
pp. 52-55 ◽  
Author(s):  
Gail L. MacLean ◽  
Andrew Stuart ◽  
Robert Stenstrom
Keyword(s):  
High Frequency ◽  
Sound Pressure ◽  
Low Frequency ◽  
Test System ◽  
Output Frequency ◽  
Frequency Effects ◽  
Adult Men ◽  
Frequency Responses ◽  
Significant Difference ◽  
Sound Pressure Levels

Differences in real ear sound pressure levels (SPLs) with three portable stereo system (PSS) earphones (supraaural [Sony Model MDR-44], semiaural [Sony Model MDR-A15L], and insert [Sony Model MDR-E225]) were investigated. Twelve adult men served as subjects. Frequency response, high frequency average (HFA) output, peak output, peak output frequency, and overall RMS output for each PSS earphone were obtained with a probe tube microphone system (Fonix 6500 Hearing Aid Test System). Results indicated a significant difference in mean RMS outputs with nonsignificant differences in mean HFA outputs, peak outputs, and peak output frequencies among PSS earphones. Differences in mean overall RMS outputs were attributed to differences in low-frequency effects that were observed among the frequency responses of the three PSS earphones. It is suggested that one cannot assume equivalent real ear SPLs, with equivalent inputs, among different styles of PSS earphones.

Pre-clinical evaluation of a virtual reality neuropsychological test system: Occurence of side effects

2000 ◽  
Author(s):  
Tibor I. Kesztyues ◽  
M. Mehlitz ◽  
E. Schilken ◽  
G. Weniger ◽  
S. Wolf ◽  
...  
Keyword(s):  
Virtual Reality ◽  
Side Effects ◽  
Clinical Evaluation ◽  
Neuropsychological Test ◽  
Test System

The Diagnosis of Lupus Anticoagulants by the Activated Partial Thromboplastin Time - The Central Role of Phosphatidyl Serine

1984 ◽  
Vol 52 (02) ◽  
pp. 172-175 ◽  
Author(s):  
P R Kelsey ◽  
K J Stevenson ◽  
L Poller
Keyword(s):  
Screening Method ◽  
Coagulation Factors ◽  
Phosphatidyl Serine ◽  
Test System ◽  
Specific Factor ◽  
Marginal Effect ◽  
Constant Composition ◽  
Lupus Anticoagulants ◽  
Different Types

SummaryLiposomes of pure phospholipids were used in a modified APTT test system and the role of phosphatidyl serine (PS) in determining the sensitivity of the test system to the presence of lupus anticoagulants was assessed. Six consecutive patients with lupus anticoagulants and seven haemophiliacs with anticoagulants directed at specific coagulation factors, were studied. Increasing the concentration of phospholipid in the test system markedly reduced the sensitivity to lupus anticoagulants but had marginal effect on the specific factor inhibitors. The same effect was achieved when the content of PS alone was increased in a vehicle liposome of constant composition.The results suggest that the lupus anticoagulants can best be detected by a screening method using an APTT test with a reagent of low PS content. The use of a reagent rich in PS will largely abolish the lupus anticoagulant’s effect on the APTT. An approach using the two different types of reagent may facilitate differentiation of lupus inhibitors from other types of anticoagulant.

Strategi Penyelenggaraan Diklat Dan Ujian Sertifikasi Keahlian Pengadaan Barang/ Jasa Pemerintah Di Lingkungan Pemerintah Kabupaten Magelang

2017 ◽  
Vol 24 (1) ◽  
pp. 87-106
Author(s):  
Wiharyanto Wiharyanto
Keyword(s):  
Education And Training ◽  
Test System ◽  
Civil Servants ◽  
Quality Of Education ◽  
Alternative Formulation ◽  
Computer Assisted ◽  
Certification Exams ◽  
The Government ◽  
And Training

The study aims to analyze about the low graduation and certification exam training participants of the procurement of goods / services of the government and its contributing factors, and formulate a strategy of education and training and skills certification exams procurement of goods / services of the government. Collecting data using the method of study documentation, interviews, and questionnaires. Is the official source of information on the structural and functional Regional Employment Board, as well as the participants of the training and skills certification exams procurement of goods / services of the government in Magelang regency government environment. Analysis using 4 quadrant SWOT analysis, to determine the issue or strategic factors in improving the quality of education and training and skills certification exams procurement of government goods / services within the Government of Magelang regency. The results show organizer position is in quadrant I, which is supporting the growth strategy, with 3 alternative formulation strategies that improve the quality of education and training and skills certification exams procurement of government goods / services, and conducts certification examination of the procurement of government goods / services with computer assisted test system (CAT). Based on the research recommendations formulated advice to the organizing committee, namely: of prospective participants of the training and skills certification exams procurement of goods / services the government should consider the motivation of civil servants, is examinees who have attended training in the same period of the year, the need for simulation procurement of goods / services significantly, an additional allocation of training time, giving sanction to civil servants who have not passed the exam, the provision of adequate classroom space with the number of participants of each class are proportional, as well as explore the evaluation of education and training and skills certification exams procurement of goods / services for Government of participants.

STUDIES ON A NON-MELATONIN PINEAL ANTI-GONADOTROPHIN

1972 ◽  
Vol 69 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Bryant Benson ◽  
Mary Jane Matthews ◽  
Alvin E. Rodin
Keyword(s):  
Solvent Extraction ◽  
Organic Solvent ◽  
Pineal Gland ◽  
Gel Filtration ◽  
Test System ◽  
Pineal Body ◽  
Physiological Effects ◽  
Organic Solvent Extraction ◽  
Gland Function

ABSTRACT Continuing investigation of pineal gland function indicates that the anti-gonadotrophic activity of this organ cannot be attributed solely to the postulated hormone melatonin, the concentration of which is negligible in the pineal body compared to quantities required to produce unequivocal physiological effects. A non-melatonin antigonadotrophic substance recently isolated from bovine pineal glands was further purified by organic solvent extraction, ultrafiltration and gel filtration. Studies of partial blockage of compensatory ovarian hypertrophy in unilaterally ovariectomized Charles River CD-1 mice indicated that this substance is significantly more potent than melatonin in this test system.

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

Results of Surveillance Studies of Infectious Fish Diseases in Freshwater Aquaculture of Ukraine

2015 ◽  
Vol 2 (2) ◽  
pp. 32-38 ◽  
Author(s):  
N. Matvienko ◽  
A. Vashchenko ◽  
I. Tsiganok ◽  
L. Buchatsky
Keyword(s):  
Rainbow Trout ◽  
Infectious Diseases ◽  
Cell Cultures ◽  
Standardized Test ◽  
Age Groups ◽  
Infectious Pancreatic Necrosis Virus ◽  
Test System ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Freshwater Aquaculture ◽  
Pancreatic Necrosis Virus

Aim. To investigate the epizootic state of fi sheries in Ukraine; to study the biological specifi cities of viral and bacterial isolates of fi sh in freshwater aquaculture. Methods. The epizootic state of fi sheries was defi ned ac- cording to the surveillance plan for fi sheries, virological (biosampling of sensitive fi sh species, virus isolation on sensitive passaged cell cultures), serological (enzyme immunoassay, virus neutralization test using sensitive passaged cell cultures) and molecular-biological (reverse transcriptase polymerase chain reaction − RTPCR) methods of investigation were used. The pathogenicity of the isolated bacteria was studied in the biosample. The identifi cation was performed using Bergey’s Manual. The express-identifi cation of bacteria was performed using the standardized test-system API 20E Bio Merieux (France). Results. The IPNV isolates of rainbow trout were fi rst isolated in the fi sheries of different forms of ownership in the western regions of Ukraine (Volyn, L’viv, Transcarpathian, Chernivtsi regions). It was demonstrated that different age groups of carp are infested with the virus in the fi sheries of L’viv, Donetsk, Chernihiv, Kyiv, and Odesa regions which testifi es to a wide spread of the virus in Ukraine. Out of fi sh infectious diseases the red spot-like disease and the swim bladder infl ammation of carp, the diseases of young trout and sturgeon were detected in the investigated fi sheries of Ukraine. Conclusions. The epizootic data were used to estimate the condition of the fi sheries in Ukraine in terms of fi sh infectious diseases. An infectious pancreatic necrosis virus, new for Ukraine, was revealed. It was found to affect rainbow trout (Oncorhynchus mykiss , Walbaum, 1792) and the spread of SVCV in carp fi sheries was demonstrated. As for bacterial fi sh diseases, the decrease in the epizootic situation was described along with considerable extension of the range of species of bacterial pathogens of fi sh. Annual systematic monitoring and measures of preventing the introduction of the agents of infectious diseases are the guarantee of protection of the specialized fi sheries of Ukraine.

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