Determination of mutagenic and antimutagenic properties of flavonoid compounds isolated from Mentha longifolia ssp. longifolia and Origanum vulgare ssp. vulgare by using AMES test system

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
Z Guvenalp ◽  
M Güllüce ◽  
M Karadayi ◽  
H Ozbek ◽  
T Arasoglu ◽  
...  
2011 ◽  
Vol 76 (9) ◽  
pp. T212-T217 ◽  
Author(s):  
Ozlem Baris ◽  
Mehmet Karadayi ◽  
Derya Yanmis ◽  
Zuhal Guvenalp ◽  
Tugba Bal ◽  
...  
Keyword(s):  
E Coli ◽  

2014 ◽  
Vol 70 (4) ◽  
pp. 599-604 ◽  
Author(s):  
Bing Wang ◽  
Ivo Achu Nges ◽  
Mihaela Nistor ◽  
Jing Liu

In this work, biochemical methane potential (BMP) tests with cellulose as a model substrate were performed with the aid of three manually operated or conventional experimental setups (based on manometer, water column and gas bag) and one automated apparatus specially designed for analysis of BMP. The methane yields were 340 ± 18, 354 ± 13, 345 ± 15 and 366 ± 5 ml CH4/g VS obtained from experimental setups with manometer, water column, gas bag, and automatic methane potential test system, which corresponded to a biodegradability of 82, 85, 83 and 88% respectively. The results demonstrated that the methane yields of cellulose obtained from conventional and automatic experimental setups were comparable; however, the methane yield obtained from the automated apparatus showed greater precision. Moreover, conventional setups for the BMP test were more time- and labour-intensive compared with the automated apparatus.


2010 ◽  
pp. 127-133
Author(s):  
Svetlana Trifunci ◽  
Dorina Ardelean

The isolation and quantitative determination of flavonoid compounds in fruit of Juniperus virginiana L. (Cupressaceae) are described. A method for the detection of those flavonoids was high performance liquid chromatography (HPLC). Rutin and kaempferol were determined in accordingly extracts and quercetin only in hydrolysated extracts.


2006 ◽  
Vol 4 (1) ◽  
pp. 22-27
Author(s):  
Petimat M Djambetova ◽  
Nina V Reutova

The plant test system soybean (Glycine max. (L.) Merill) line T219 turned out to be more sensitive than standart Ames test for evaluation of the mutagenic effect of soil, contaminated by products of combustion and domestic cottage processing of oil. It is preferable to use plant test systems for such investigations because they are more sensitive, simple and inexpensive in comparison with microbial ones.


2018 ◽  
Vol 33 (2) ◽  
pp. 70-73
Author(s):  
K.A. Mirzaeva ◽  
◽  
R.Z. Zeynalov ◽  
K.E. Magomedov ◽  
◽  
...  
Keyword(s):  

2019 ◽  
Vol 19 (4) ◽  
pp. 242-250
Author(s):  
A. A. Borzov ◽  
A. A. Оvsepyan ◽  
E. I. Katorkina ◽  
E. O. Anisimova ◽  
M. V. Lykov

Glioblastoma is the most common and most aggressive type of brain tumor, with an almost 100 % mortality rate over 5 years. The search for new effective approaches to the treatment of this disease requires the development of adequate experimental models.Objective: to develop and put into practice an orthotopic model of mouse glioblastoma.Materials and methods: GLi-261 mouse glioma cells were orthotopically inoculated into the putamen of C57Bl/6 mice brain. Tumor dynamics was investigated by Preclinical MRI System 7.0T/17cm (Flexiscan) highfield magnetic resonance imager (MR Solutions, UK). Temcital® (temozolomide) was used as a positive control in the treatment of experimental glioblastoma. The neurological status of animals in the course of tumour development was assessed by specific tests.Results: a GLi-261 cell-based mouse glioblastoma orthotopic model was developed using stereotactic equipment for accurate inoculation of tumour cells, magnetic resonance imaging for non-invasive determination of tumour volume and dynamics, and special tests for determination of the neurological status of the biological test systems. This model was used to demonstrate the effectiveness of temozolomide (the «gold standard» for glioblastoma treatment).Conclusions: this model has been introduced into practice at the IBC Generium, LLC, and can be used as an in vivo test system for preclinical evaluation of efficacy of new antitumour drugs being developed, as well as brain cancer treatment regimens using combination therapy.


2006 ◽  
Vol 306-308 ◽  
pp. 1509-1514 ◽  
Author(s):  
Jing Feng ◽  
Qian Sheng ◽  
Chao Wen Luo ◽  
Jing Zeng

It is very important to study the pristine stress field in Civil, Mining, Petroleum engineering as well as in Geology, Geophysics, and Seismology. There are various methods of determination of in-situ stress in rock mass. However, hydraulic fracturing techniques is the most convenient method to determine and interpret the test results. Based on an hydraulic fracturing stress measurement campaign at an underground liquefied petroleum gas storage project which locates in ZhuHai, China, this paper briefly describes the various uses of stress measurement, details of hydraulic fracturing test system, test procedure adopted and the concept of hydraulic fracturing in arriving at the in-situ stresses of the rock mass.


2003 ◽  
Vol 86 (2) ◽  
pp. 236-240 ◽  
Author(s):  
Lieve Okerman ◽  
Katia De Wasch ◽  
Jan Van Hoof ◽  
Walter Smedts

Abstract Parallux®, a solid-phase fluorescence immunoassay (SPFIA) developed for antibiotic residue detection in milk, was used for analysis of bovine and porcine kidney tissue. Four tetracyclines, 2 broad-spectrum cephalosporins, 3 beta-lactam antibiotics, and cephapirin were detected in one run after minimal sample preparation. This commercially available test system is designed as cartridges, each with a combination of 1–4 tests. One cartridge can be used to detect 4 analytes in the same sample, or 1 or 2 analytes in different samples. The cartridge with the combination tetracyclines–ceftiofur–penicillin–cephapirin was selected because tetracyclines, beta-lactam antibiotics as well as cephalosporins, are registered for oral or parenteral use in bovines and pigs in Europe. The test is qualitative and is recommended only for screening. Tetracycline, oxytetracycline, chlortetracycline, and doxycycline were easily detected at 300 ppb with the tetracyclines channel; ceftiofur at 1000 ppb and cefquinome at 200 ppb with the ceftiofur channel; penicillin G, ampicillin, and amoxicillin at 50 ppb with the penicillin channel; and cephapirin at 100 ppb with the cephapirin channel. These levels are equal to or lower than the corresponding maximal residue limits in kidney tissue. Cephalexin was not detected. The SPFIA test can be used as an alternative to classical inhibition tests and for post-screening inhibitor- positive kidneys, because it detects 3 specific groups of antibiotics, which enables selection of specific confirmatory methods for identification and quantification.


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