scholarly journals Regulation of the Discoidin I gamma gene in Dictyostelium discoideum: identification of individual promoter elements mediating induction of transcription and repression by cyclic AMP.

1990 ◽  
Vol 10 (8) ◽  
pp. 4080-4088 ◽  
Author(s):  
F Vauti ◽  
P Morandini ◽  
J Blusch ◽  
A Sachse ◽  
W Nellen
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Base Pair ◽  
Regulation Of Gene Expression ◽  
Promoter Elements ◽  
Down Regulation ◽  
Heterologous Promoter ◽  
Sequence Element ◽  
Pair Sequence

We dissected the promoter of the developmentally induced and cyclic AMP-repressed discoidin I gamma gene and identified a sequence element essential for developmental induction. Transfer of the element to an inactive heterologous promoter demonstrated that this sequence is sufficient to confer expression in axenically growing cells and to induce gene activity in development after growth on bacteria. A 16-base-pair sequence within this element was shown to be sufficient for induction in the discoidin promoter context and was used to reactivate different truncated promoter constructs. This led to the localization of an element necessary for down regulation of gene expression by extracellular cyclic AMP.

Regulation of the Discoidin I gamma gene in Dictyostelium discoideum: identification of individual promoter elements mediating induction of transcription and repression by cyclic AMP

1990 ◽  
Vol 10 (8) ◽  
pp. 4080-4088
Author(s):  
F Vauti ◽  
P Morandini ◽  
J Blusch ◽  
A Sachse ◽  
W Nellen
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Base Pair ◽  
Regulation Of Gene Expression ◽  
Promoter Elements ◽  
Down Regulation ◽  
Heterologous Promoter ◽  
Sequence Element ◽  
Pair Sequence

We dissected the promoter of the developmentally induced and cyclic AMP-repressed discoidin I gamma gene and identified a sequence element essential for developmental induction. Transfer of the element to an inactive heterologous promoter demonstrated that this sequence is sufficient to confer expression in axenically growing cells and to induce gene activity in development after growth on bacteria. A 16-base-pair sequence within this element was shown to be sufficient for induction in the discoidin promoter context and was used to reactivate different truncated promoter constructs. This led to the localization of an element necessary for down regulation of gene expression by extracellular cyclic AMP.

Translational down-regulation of gene expression

1998 ◽  
Vol 5 ◽  
pp. 122
Author(s):  
D.B. Thomason ◽  
J. Wong ◽  
L. Fu ◽  
E. Schneider ◽  
Z. Ku ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulation Of Gene Expression ◽  
Down Regulation

Pharmacological characterization of cyclic AMP receptors mediating gene regulation in Dictyostelium discoideum

1986 ◽  
Vol 6 (7) ◽  
pp. 2402-2408
Author(s):  
B Haribabu ◽  
R P Dottin
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Cell Surface ◽  
Dictyostelium Discoideum ◽  
Cell Surface Receptor ◽  
Intracellular Camp ◽  
Response Curves ◽  
Pharmacological Characterization ◽  
Surface Receptor ◽  
Extracellular Molecules

Extracellular molecules regulate gene expression in eucaryotes. Exogenous cyclic AMP (cAMP) affects the expression of a large number of developmentally regulated genes in Dictyostelium discoideum. Here, we determine the specificity of the receptor(s) which mediates gene expression by using analogs of cAMP. The order of potency with which these analogs affect the expression of specific genes is consistent with the specificity of their binding to a cell surface receptor and is distinct from their affinity for intracellular cAMP-dependent protein kinase. Dose-response curves with cAMP and adenosine 3',5'-monophosphorothioate, a nonhydrolyzable analog, revealed that the requirement for high concentrations of exogenous cAMP for regulating gene expression is due to the rapid degradation of cAMP by phosphodiesterase. The addition of low concentrations of cAMP (100 nM) or analogs in pulses also regulates gene expression. Both the genes that are positively regulated by exogenous cAMP and the discoidin gene, which is negatively regulated, respond to cAMP analogs to the same degree. Genes expressed in prespore or prestalk cells are also similarly regulated. These data suggest that the effects are mediated through the same receptor. The specificity of this receptor is indistinguishable from that of the well-characterized cell surface cAMP receptor.

Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP

1989 ◽  
Vol 9 (11) ◽  
pp. 4660-4669
Author(s):  
J Pavlovic ◽  
B Haribabu ◽  
R P Dottin
Keyword(s):  
Signal Transduction ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Regulatory Element ◽  
Response Sequence ◽  
Base Pairs ◽  
Camp Response Element ◽  
Sequence Element ◽  
Cis Acting ◽  
Gene Coding

The signal transduction pathways that lead to gene induction are being intensively investigated in Dictyostelium discoideum. We have identified by deletion and transformation analysis a sequence element necessary for induction of a gene coding for uridine diphosphoglucose pyrophosphorylase (UDPGP1) of D. discoideum in response to extracellular cyclic AMP (cAMP). This regulatory element is located 380 base pairs upstream of the transcription start site and contains a G+C-rich partially palindromic sequence. It is not required for transcription per se but is required for induction of the gene in response to the stimulus of extracellular cAMP. The cAMP response sequence is also required for induction of the gene during normal development. A second A+T-rich cis-acting region located immediately downstream of the cAMP response sequence appears to be essential for the basal level of expression of the UDPGP1 gene. The position of the cAMP response element coincides with a DNase I-hypersensitive site that is observed when the UDPGP1 gene is actively transcribed.

Resolving the Sequence-Dependent Stiffness of DNA Using Cyclization Experiments and a Computational Rod Model

2007 ◽  
Author(s):  
Sachin Goyal ◽  
Noel C. Perkins ◽  
Jens-Christian Meiners
Keyword(s):  
Gene Expression ◽  
Experimental Data ◽  
Material Properties ◽  
Base Pair ◽  
Biological Processes ◽  
Significant Challenge ◽  
Sequence Dependent ◽  
Rod Model ◽  
Structural Deformations ◽  
Pair Sequence

Structural deformations of DNA play a central role in many biological processes including gene expression. The structural deformations are sensitive to the material properties of the molecule and these, in turn, vary along the molecule’s length according to its base-pair sequence. Example ‘sequence-dependent’ material properties include the stress-free curvature and the stiffness for bending and torsion. Separating and quantifying these sequence-dependent properties from experimental data remains a significant challenge as they often work in unison in nature. In this paper, we offer a method for resolving and quantifying the sequence-dependent stiffness of DNA from cyclization (loop closure) experiments using a computational rod model of the molecule.

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