Field monitoring of plant-growth-promoting rhizobacteria by colony immunoblotting

2011 ◽  
Vol 57 (11) ◽  
pp. 914-922 ◽  
Author(s):  
Ganisan Krishnen ◽  
Mihály L. Kecskés ◽  
Michael T. Rose ◽  
Peter Geelan-Small ◽  
Khanok-on Amprayn ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Plant Growth Promoting Rhizobacteria ◽  
Potential Method ◽  
Soil Microflora ◽  
Plant Growth Promoting Bacteria ◽  
Whatman Filter Paper ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Filter Papers

Inoculant plant-growth-promoting bacteria are emerging as an important component of sustainable agriculture. There is a need to develop inexpensive methods for enumerating these organisms after their application in the field, to better understand their survival and impacts on yields. Immunoblotting is one potential method to measure viable cells, but the high cost of the conventionally used nylon membranes makes this method prohibitive. In this study, less expensive alternative materials such as filter papers, glossy photo papers, and transparencies for the purpose of colony immunoblotting were evaluated and the best substance was chosen for further studies. Whatman filter paper No. 541 combined with a 0.01 mol·L–1 H2SO4 rinsing step gave similar results to nylon membranes but <20% of the overall cost of the original colony immunoblotting assay. The application of the modified immunoblot method was tested on nonsterile clay soil samples that were spiked with high numbers (>107 CFU·g–1) of the plant-growth-promoting bacteria Pseudomonas fluorescens , Azospirillum brasilense , or Rhizobium leguminosarum . The modified protocol allowed the identification and recovery of over 50% of the inoculated cells of all three strains, amidst a background of the native soil microflora. Subsequently, the survival of P. fluorescens was successfully monitored for several months after application to field-grown rice at Jerilderie, New South Wales, Australia, thus validating the procedure.

scholarly journals Mecanismos de acción de las rizobacterias promotoras del crecimiento vegetal.

2011 ◽  
Vol 12 (2) ◽  
pp. 159 ◽  
Author(s):  
Mauricio Camelo R. ◽  
Sulma Paola Vera M. ◽  
Ruth Rebeca Bonilla B.
Keyword(s):  
Plant Growth ◽  
High Efficiency ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting Bacteria ◽  
Toxic Substances ◽  
Pests And Diseases ◽  
Modern Agriculture ◽  
Plant Growth Promoting ◽  
Production Cycles ◽  
Growth Promoting

<p>La dinámica poblacional de la especie humana ha llevado a que la explotación de los recursos naturales, en búsqueda de suplir las necesidades alimenticias de los miles de millones de personas que habitan el planeta. Esta necesidad ha llevado a la utilización de materiales de alta eficiencia en la agricultura, variedades vegetales resistentes a plagas y enfermedades con ciclos de producción más cortos, agroquímicos que surten las necesidades nutricionales y provean protección frente factores bióticos adversos (plagas y enfermedades). Sin embargo, estas estrategias utilizadas en la agricultura moderna han generado impactos ambientales negativos que aún no comprendemos. La contaminación de aguas freáticas, eutrofización, aumento de gases de invernadero y acumulación de sustancias toxicas en la cadena trófica, son algunos de los graves problemas que se presentan por el uso indiscriminado de agroquímicos. Como alternativa a la utilización de estas sustancias, se ha propuesto el uso de bacterias rizosféricas que tienen reconocida acción sobre el crecimiento y desarrollo vegetal (PGPR, por sus siglas en ingles). Estas bacterias son capaces de estimular el desarrollo de las plantas de manera directa e indirecta y poseen una serie de mecanismos complejos que interactúan entre sí para establecer relaciones benéficas, especialmente con las raíces de las plantas objetivo. El estudio y entendimiento de las PGPR han sido temas de gran importancia en muchas investigaciones a nivel mundial, por esta razón esta revisión tiene por objetivo hacer una revisión parcial para dar a conocer los mecanismos que poseen las rizobacterias promotoras del crecimiento vegetal en el desarrollo de las plantas, así como el papel que desempeñan en el ciclaje de nutrientes.</p><p> </p><p><strong>Mechanisms of action of plant growth promoting rhizobacteria.</strong></p><p>The population dynamics of the human race has led to the exploitation of natural resources in search of a way to meet the nutritional needs of the billions of people inhabiting the planet. This need has led to the use of high-efficiency materials in agriculture, plant varieties with shorter production cycles that are also resistant to pests and diseases, and chemicals that provide protection against biotic factors (pests and disease), additionally the nutrients required to grow plants. However, the strategies used in modern agriculture have led to negative environmental impacts that we have yet to fully understand. Groundwater contamination, eutrophication, increased greenhouse gases, and the accumulation of toxic substances in the food chain are some of the serious problems that have arisen worldwide due to the indiscriminate use of agrochemicals. As an alternative to the use of these substances, the use of rhizopheric bacteria has been proposed owing to its known action as plant growth- promoting bacteria (PGPB). These bacteria are able to stimulate plant growth directly and indirectly and have several complex mechanisms that interact with each other to establish beneficial relationships, especially with the roots of target plants. The study and understanding of PGPR have been the subjects of great importance in many studies at a global level. This review, therefore, aims to better understand the mechanisms of plant growth-promoting rhizobacteria on plant development and their role in nutrient cycling.</p>

Rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce

1996 ◽  
Vol 42 (3) ◽  
pp. 279-283 ◽  
Author(s):  
T. C. Noel ◽  
C. Sheng ◽  
C. K. Yost ◽  
R. P. Pharis ◽  
M. F. Hynes
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Direct Growth ◽  
Early Seedling ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Early seedling root growth of the nonlegumes canola (Brassica campestris cv. Tobin, Brassica napus cv. Westar) and lettuce (Lactuca saliva cv. Grand Rapids) was significantly promoted by inoculation of seeds with certain strains of Rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. The growfh-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. Auxotrophic Rhizobium mutants requiring tryptophan or adenosine (precursors for indole-3-acetic acid and cytokinin synthesis, respectively) did not promote growth to the extent of the parent strain. The findings of this study demonstrate a new facet of the Rhizobium–plant relationship and that Rhizobium leguminosarum can be considered a plant growth-promoting rhizobacterium (PGPR).Key words: Rhizobium, plant growth-promoting rhizobacteria, PGPR, indole-3-acetic acid, cytokinin, roots, auxotrophic mutants.

scholarly journals Comparative Effects of Bio-Wastes in Combination with Plant Growth-Promoting Bacteria on Growth and Productivity of Okra

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2065
Author(s):  
Hammad Anwar ◽  
Xiukang Wang ◽  
Azhar Hussain ◽  
Muhammad Rafay ◽  
Maqshoof Ahmad ◽  
...  
Keyword(s):  
Plant Growth ◽  
Soil Health ◽  
Organic Amendments ◽  
Plant Growth Promoting Rhizobacteria ◽  
Fruit Weight ◽  
Growth And Yield ◽  
Plant Growth Promoting Bacteria ◽  
Randomized Design ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria with multiple growth-promoting traits play a significant role in soil to improve soil health, crop growth and yield. Recent research studies have focused on the integration of organic amendments with plant growth-promoting rhizobacteria (PGPR) to enhance soil fertility and reduce the hazardous effects of chemical fertilizers. This study aims to evaluate the integrated application of biochar, compost, fruit and vegetable waste, and Bacillus subtilis (SMBL 1) to soil in sole application and in combined form. The study comprises eight treatments—four treatments without inoculation and four treatments with SMBL 1 inoculation in a completely randomized design (CRD), under factorial settings with four replications. The results indicate that the integrated treatments significantly improved okra growth and yield compared with sole applications. The integration of SMBL 1 with biochar showed significant improvements in plant height, root length, leaf chlorophyll a and b, leaf relative water content, fruit weight, diameter and length by 29, 29, 50, 53.3, 4.3, 44.7 and 40.4%, respectively, compared with control. Similarly, fruit N, P and K contents were improved by 33, 52.7 and 25.6% and Fe and Zn in shoot were 37.1 and 35.6%, respectively, compared with control. The results of this study reveal that the integration of SMBL 1 with organic amendments is an effective approach to the sustainable production of okra.

Effets de l'inoculation avec des souches deRhizobium leguminosarumbiovartrifoliisur la croissance du blé dans deux sols du Maroc

2001 ◽  
Vol 47 (6) ◽  
pp. 590-593 ◽  
Author(s):  
Abdelaly Hilali ◽  
Danielle Prévost ◽  
William J Broughton ◽  
Hani Antoun
Keyword(s):  
Plant Growth ◽  
Dry Matter ◽  
Rhizobium Leguminosarum ◽  
Agricultural Soils ◽  
Plant Growth Promoting Rhizobacteria ◽  
Silty Clay ◽  
Rotation System ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Pgpr Activity

One hundred strains of Rhizobium leguminosarum bv. trifolii were isolated from roots of wheat cultivated in rotation with clover in two different regions of Morocco. The isolates were first screened for their effect on the growth of the cultivar Rihane of wheat cultivated in an agricultural soil under greenhouse conditions. After 5 weeks of growth, 14 strains stimulating the fresh or dry matter yield of shoots were selected and used in a second pot inoculation trial performed with two different agricultural soils. The results show that the strains behaved differently according to the soil used. In the loamy sand Rabat, strain IAT 168 behaved potentially like a plant growth promoting rhizobacteria (PGPR), as indicated by the 24% increases (P < 0.1) observed in wheat shoot dry matter and grain yields. In the silty clay Merchouch, no PGPR activity was observed, and 6 strains showed a significant deleterious effect on yields. These observations suggest that it is very important in a crop rotation system to choose a R. leguminosarum bv. trifolii strain that is effective with clover and shows PGPR activity with wheat to avoid deleterious effects on wheat yields.Key words: deleterious bacteria, PGPR (plant growth promoting rhizobacteria), Trifolium alexandrinum, Triticum aestivum.

scholarly journals Plant Growth Promoting Rhizobacteria (PGPRs) Alter Plant Host Somatic Mutation Frequencies

2019 ◽  
Vol 5 (03) ◽  
pp. 149-154
Author(s):  
Jasmine M. Shah ◽  
Aparna C. ◽  
Pallavi S. Nair
Keyword(s):  
Plant Growth ◽  
Somatic Mutation ◽  
Post Infection ◽  
Rhizobium Leguminosarum ◽  
Somatic Mutations ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pathogenic Strain ◽  
Plant Host ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting rhizobacteria (PGPRs) are a group of soil bacteria which can induce positive growth in plants by different mechanisms. This work intends to find the effect of PGPRs on two classes of somatic mutations in the host, frame shift mutation (FSM) and somatic homologous recombination (SHR) and, compare the same with that of a pathogen. Somatic mutations in plants are important as they are an adaptation strategy to overcome stressful conditions and also get passed on to the next generations. The mutation detector Arabidopsis thaliana lines carrying a non-functional β-glucuronidase gene (GUS) were used to score the mutation events. One day-old mutant seedlings were co-cultivated with the PGPRs (Rhizobium leguminosarum and Pseudomonas fluorescens) and the pathogenic strain (P. syringae) for two different post-infection durations (4 h and 48 h). A reversion of the mutated GUS to its functional form resulted in blue spots in the host plant. Based on the number of blue spots seen, the mutation frequencies were estimated. An increase in FSM was observed in plants co-cultivated with R. leguminosarum for 4 h as well as 48 h. R. leguminosarum suppressed SHR frequency 4 h-post infection, which significantly increased at 48 h. In contrast, P. fluorescens infection lead to a temporal suppression of FSM and induction of SHR at 4 h. Subsequently, the SHR rates reduced significantly, i.e. lower than the uninfected controls at 48 h. The pathogenic strain P. syringae temporally increased FSM in plants and also enhanced SHR rates in plants 4 h post-infection, which also subsequently reduced 48 h post infection. To the best of our knowledge, there are no other reports comparing the effect of PGPRs on host somatic mutation rates.

scholarly journals Induction of Bacterial Canker Resistance in Tomato Plants Using Plant Growth Promoting Rhizobacteria

2019 ◽  
Vol 13 (1) ◽  
pp. 215-222 ◽  
Author(s):  
Yuliya Kolomiiets ◽  
Ivan Grygoryuk ◽  
Artur Likhanov ◽  
Lyudmila Butsenko ◽  
Yaroslav Blume
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Cell Suspensions ◽  
Systemic Resistance ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Canker ◽  
Tomato Plants ◽  
High Productivity ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background: By inducing the production of inhibitory allelochemicals and mechanisms of systemic resistance plant growth promoting bacteria (PGPB) help plants to cope with stresses. Materials and Methods: In this study cell suspensions of Bacillus subtilis, Pseudomonas fluorescens or Azotobacter chroococcum were used to test the efficacy of these PGPB in inducing resistance in tomato (Lycopersicon esculentum Mill) against Clavibacter michiganensis subsp michiganensis, a bacteria known to cause canker disease. To test this hypothesis, seedlings of Chaika variety, characterized by short growing, early-ripening, high productivity and resistance against fusarium and the C. michiganensis strain ІZ-38 isolated in Kyiv were employed. Results and Conclusion: The use of cell suspensions of the PGPB B. subtilis, A. chroococcum or P. fluorescens induced an increment in the resistance of tomato plants against the causative agent of bacterial canker (C. michiganensis subsp. michiganensis) by 42–50%. PGPB in fact promoted in C. michiganensis infected tomato plants: i) the accumulation of chlorophyll a and b and carotenoids; ii) the thickening of the upper and lower epidermis of leaves; iii) the deposition of biopolymers with protective properties in epidermal cells; iv) the activity of the peroxidase enzyme and v) the net productivity of photosynthesis.

scholarly journals Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum

2021 ◽  
Vol 6 (2) ◽  
pp. 255-263
Author(s):  
Indah Juwita Sari ◽  
Indria Wahyuni ◽  
Rida Oktorida Khastini ◽  
Ewi Awaliyati ◽  
Andriana Susilowati ◽  
...  
Keyword(s):  
Plant Growth ◽  
Capsicum Annuum ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting Bacteria ◽  
Dual Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.

scholarly journals Alleviation of Salt Stress on Wheat (Triticum aestivum L.) by Plant Growth Promoting Bacteria strains Bacillus halotolerans MSR-H4 and Lelliottia amnigena MSR-M49

2020 ◽  
pp. 44-58
Author(s):  
Ibrahim El-Akhdar ◽  
Tamer Elsakhawy ◽  
Hanaa A. Abo-Koura
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Crop Production ◽  
Halophilic Bacteria ◽  
Wheat Plant ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting rhizobacteria (PGPR) application could reduce the use of synthetic fertilizers and increase the sustainability of crop production. Halophilic bacteria that have PGPR characteristics can be used in different environmental stresses. Two different strains isolated, purified, characterized as a PGPRs and phylogenetic identification using 16sRNA which was revealed to be closest matched at 99% with Bacillus halotolerans and Lelliottia amnigena. The isolates possessed plant growth promoting properties as exopolysaccharides (EPS) and indole acetic acid (IAA) production, Bacillus halotolerans had the ability to fix elemental nitrogen and the two strains have the ability to P-solubilization. Furthermore, the strains were evaluated in alleviation of different levels of salt stress on wheat plant at two experiments (Pots and a Field). Strains under study conditions significantly increased the plant height, straw dry weight (DW g plant-1), spike number, 1000 grain DW recorded 31.550 g with Lelliottia amnigena MSR-M49 compared to un-inoculated and other strain in field,  grain yield recorded 2.77 (ton fed-1) with Lelliottia amnigena  as well as N% and protein content in grains recorded 1.213% and 6.916 respectively with  inoculation with Lelliottia amnigena,  also, spikes length, inoculated wheat show reduction in both proline accumulation in shoots and roots especially with Lelliottia amnigena recorded 2.79 (mg g-1DW), inoculation significantly increased K+ in root-shoot, K+/Na+ in root-shoot and reduced Na+ in root-shoot compared with control. This confirmed that this consortium could provide growers with a sustainable approach to reduce salt effect on wheat production.

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