scholarly journals Synthesis, Characterization and Antimicrobial Activity of d8-10 Metal Complexes of some 2-Substituted-1H-Benzimidazoles

1999 ◽  
Vol 6 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Bahri Ülküseven ◽  
Aydin Tavman ◽  
Gülten Ötük
Keyword(s):  
Antimicrobial Activity ◽  
Metal Complexes ◽  
Staphylococcus Epidermidis ◽  
Shigella Flexneri ◽  
Analytical Data ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

The metal complexes of nine 2-substituted-1H-benzimidazoles (I-IX) with Ni(II), Pd(II), Cu(II), Ag(I), Zn(II) salts were synthesized. The compounds were characterized by melting point, analytical data, IR spectroscopy and magnetic susceptibility. The antimicrobial activity of the compounds was determined by the disk diffusion method in Mueller-Hinton Agar on Staphylococcus aureusATCC 6538, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 8739, Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 1539, Salmonella typhi, Shigella flexneri, Proteus mirabilis, Candida albicans ATCC 10231. Cu(II)and Ag(I)complexes of II, III and IV showed considerable activity against S. aureus, S. epidermidis, Ps. aeruginosa, S. typhi, Sh. flexneri and C. albicans microorganisms, the ligands themselves having no effect.

scholarly journals Synthesis and Antimicrobial Assessment of Fe3+ Inclusion Complex of p-tert-Butylcalix[4]arene Diamide Derivative

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Anwar Ali Chandio ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Fakhar N. Memon ◽  
Qadeer Khan Panhwar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Ft Ir ◽  
Bacteria And Fungi

Present study deals with the synthesis of the p-tert-butylcalix[4]arene diamide derivative as ligand (L) and its Fe3+ complex, followed by its characterization using TLC and FT-IR, while UV-Vis and Job’s plot study were performed for complex formation. Antimicrobial activity of the derivative (L) and its metal complex was carried out by the disc diffusion method against bacteria (Escherichia coli and Staphylococcus albus) and fungi (R. stolonifer). Different concentrations of the derivative (L) (6, 3, 1.5, 0.75, and 0.37 μg/mL) and its Fe3+ complex were prepared, and Mueller–Hinton agar was used as the medium for the growth of microorganisms. Six successive dilutions of the derivative (L) and Fe3+ complex were used against microorganisms. Two successive dilutions (6 and 3 μg/mL) of the derivative (L) showed antibacterial action against both Gram-positive and Gram-negative bacteria. In addition, three successive dilutions (6, 3, and 1.5 μg/mL) of the derivative (L) showed antifungal activity. However, all of six dilutions of the Fe3+ complex showed antimicrobial activity. Derivative (L) showed 3 and 1.5 μg/mL minimum inhibitory concentrations (MIC) against bacteria and fungi, respectively. On the contrary, its Fe3+ complex showed 0.37 μg/mL value of MIC against bacteria and fungi. Hence, Fe3+ complex of the derivative (L) was found to be a more effective antimicrobial agent against selected bacteria and fungi than the diamide derivative (L).

scholarly journals Prevalence and antibiotic resistance of Campylobacter coli isolated from broiler farms in the Marrakesh Safi region, Morocco

2020 ◽  
Vol 13 (9) ◽  
pp. 1892-1897
Author(s):  
Raja Asmai ◽  
Bouchra Karraouan ◽  
Khadija Es-Soucratti ◽  
Houda En-Nassiri ◽  
Brahim Bouchrif ◽  
...  
Keyword(s):  
Poultry Meat ◽  
Diffusion Method ◽  
Campylobacter Coli ◽  
Disk Diffusion Method ◽  
Poultry Products ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Control Procedures ◽  
Susceptibility Profiles ◽  
Campylobacter Spp

Background and Aim: Campylobacteriosis is a common foodborne disease epidemiologically linked to the consumption of poultry products. However, other sources, such as raw or contaminated milk, contaminated water or ice, contact with infected livestock, and pets, are reported. This study aimed to evaluate the prevalence and resistance to microbial resistance of Campylobacter coliM in broiler farms in the region of Marrakesh Safi, Morocco. Materials and Methods: The study was conducted between May and December 2017 and involved 35 broiler farms. One hundred and five cloacal swabs were collected from the eight provinces in the region of Marrakesh Safi, Morocco. Bacteriology method NM ISO/TS 10272-3: 2013 was used to isolate and identify Campylobacter spp. Molecular identification (polymerase chain reaction) was used for confirmation. A disk diffusion method on Mueller-Hinton agar was used for susceptibility testing. Five antibiotic agents, including first-line drugs, were evaluated. Results: Among 105 samples, 71.4% (75/105) were positive for Campylobacter spp. test and 56% (42/75) of isolates belonged to the species coli. Susceptibility profiles showed that 95.2% of C. coli strains were resistant to ampicillin, 92.8% to erythromycin and tetracycline, 85.7% to ciprofloxacin, and 7.1% to gentamicin. Conclusion: This study underlines the need to strengthen implementation of specific control procedures to decrease contamination of poultry meat with Campylobacter spp. and to reduce the use of antibiotics in the poultry sector.

Antimicrobial Studies of Microwave Assisted Copper-Chitosan Nanocomposites

2018 ◽  
Vol 24 (8) ◽  
pp. 5556-5559
Author(s):  
M Meenakshi ◽  
P Nithya ◽  
Krishnendu Biswas
Keyword(s):  
Escherichia Coli ◽  
Ethylene Glycol ◽  
Diffusion Method ◽  
Cu Nanoparticles ◽  
Disk Diffusion Method ◽  
Microwave Assisted ◽  
Antimicrobial Studies ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Microwave Assisted Method

Copper-chitosan nanocomposite (Cu-CS NC) was prepared by incorporating copper onto chitosan using ethylene glycol by microwave assisted method. Ethylene glycol was used as a solvent as well as a reductant for preparing thenanocomposites. The antibacterial activity was tested against Bacillus cereus, Staphylococcus aureus, Escherichia coli and Proteus mirabilis by Mueller-Hinton agar disk-diffusion method. The observed activity was found to be superior when compared to bare Cu nanoparticles for most of the bacteria.

scholarly journals Antimicrobial Activity of Divaricatic Acid Isolated from the Lichen Evernia mesomorpha against Methicillin-Resistant Staphylococcus aureus

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3068 ◽  
Author(s):  
Jong Oh ◽  
Yi Kim ◽  
Hyo-Seung Gang ◽  
Jin Han ◽  
Hyung-Ho Ha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Enterococcus Faecium ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Acetone Extracts ◽  
Ethyl Acetate Extracts

One hundred and seventy seven acetone extracts of lichen and 258 ethyl acetate extracts of cultured lichen-forming fungi (LFF) were screened for antimicrobial activity against Staphylococcus aureus and Enterococcus faecium using a disk diffusion method. Divaricatic acid was isolated from Evernia mesomorpha and identified by LC-MS, 1H-, 13C- and DEPT-NMR. Purified divaricatic acid was effective against Gram + bacteria, such as Bacillus subtilis, Staphylococcus epidermidis, Streptococcus mutans, and Enterococcus faecium, with the minimum inhibitory concentration (MIC) values ranging from 7.0 to 64.0 μg/mL, whereas vancomycin was effective in the MICs ranging from 0.78 to 25.0 μg/mL. Interestingly, the antibacterial activity of divaricatic acid was higher than vancomycin against S. epidermidis and E. faecium, and divaricatic acid was active against Candida albicans. In addition, divaricatic acid was active as vancomycin against S. aureus (3A048; an MRSA). These results suggested that divaricatic acid is a potential antimicrobial agent for the treatment of MRSA infections.

Dicentra scandens (D.Don) Walp. - A Potential Source of Antimicrobial Agent

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Koirala Pramila ◽  
Singh Bimala
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Methanol Extract ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Ms Analysis

Dicentra scandens (D.Don) Walp. locally called as ‘Jogi Lahara’ belongs to the Family Fumariaceae and is used in traditional medicine in Sikkim, a North Eastern state of India. The present study investigated the antimicrobial properties of aqueous and methanol extracts of Dicentra scandens (D.Don) Walp. against some test Gram negative and Gram positive bacteria. Using agar well diffusion method, aqueous and methanol extracts of roots of Dicentra scandens were tested against Escherichia coli (MTCC 1089), Klebsiella pneumoniae (MTCC 3384), Pseudomonas aeruginosa (MTCC 1034), Proteus vulgaris (MTCC 742), Salmonella typhi (MTCC 733), Shigella flexneri (MTCC 1457), Vibrio cholerae O139(MTCC 3906), Bacillus cereus (MTCC 6840) and Staphylococcus aureus (MTCC 7443). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also evaluated. Promising antimicrobial activity was exhibited by methanol extract of Dicentra scandens. The methanol extract was further characterized by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The GC-MS analysis revealed 29 compounds and the major compounds detected were Protopine (53.78%) and Corydine (18.20%). Thus, the alkaloids are predominant phytoconstituents of the extract and could be attributed to its antimicrobial activity. The results of the present study indicate that, Dicentra scandens can be a source of potential antimicrobial agent and can be explored further for its therapeutic use.

Composition and Antimicrobial Activity of the Essential Oil of Dicyclophora persica Boiss. from Iran

2006 ◽  
Vol 61 (5-6) ◽  
pp. 315-318 ◽  
Author(s):  
Peyman Salehi ◽  
Ali Sonboli ◽  
Fatemeh Mohammadi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Strong Inhibition ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Total Oil

The chemical composition of the essential oil of Dicyclophora persica Boiss. was identified by GC and GC-MS analysis. The analysis of the oil resulted in the identification of forty-five components constituting 98.6% of the total oil. The main constituents were α-pinene (31.5%), (Z)-β-ocimene (23.3%), p-cymene (6.7%) and (E)-β-ocimene (5.4%). The antimicrobial activity of the oil was tested by the disk diffusion method against four Gram-positive (Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis and Enterococcus faecalis) and three Gram-negative (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) bacteria together with a fungus (Aspergillus niger). The oil showed strong inhibition activity toward all the tested microorganisms except for Pseudomonas aeruginosa.

scholarly journals Biofilm Formation and Antimicrobial Susceptibility of Non-Diphtheriae Corynebacterium Strains Isolated from Blood Cultures: First Report from Turkey

2021 ◽  
Author(s):  
Sinem Özdemir ◽  
Okan Aydoğan ◽  
Fatma Köksal Çınar
Keyword(s):  
Antimicrobial Susceptibility ◽  
Biofilm Formation ◽  
Blood Cultures ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Biofilm Production ◽  
Mueller Hinton ◽  
Corynebacterium Striatum ◽  
Mueller Hinton Agar ◽  
Resistance Rates

Objective: Non-diphtheriae Corynebacterium strains have been recognized as important pathogens after decades of confusion regarding their microbiological classification and clinical significance. The aim of this study was to identify non-diphtheriae Corynebacterium strains and the prevalence of biofilm formation and antimicrobial resistance. Method: In total, 126 non-diphtheriae Corynebacterium strains were isolated from blood cultures of inpatients with bacteremia in our hospital between January 2015 and January 2020. Blood cultures were analyzed with the Bactec-9120 system. Strains were identified using MALDI-TOF MS (Bruker Daltonics, Germany). Antimicrobial susceptibilities were determined using the Kirby-Bauer disk diffusion method on a Mueller-Hinton agar and evaluated according to EUCAST standards. Biofilm formation was assessed with the Congo Red Agar method. Results: Corynebacterium striatum and Corynebacterium matruchotii were the most prevalent with 29 and 26 isolates, respectively. Biofilm production was detected in 62.06% (18/29) of C. striatum, in 53.8% (14/26) of C. matruchotii, in 50% (9/18) of Corynebacterium afermentans, 50% (6/12) of Corynebacterium amycolatum, and in 46% (7/15) of Corynebacterium jeikeium strains. Among the five most prevalent strains, we found a high biofilm rate of 54%. The resistance rates to penicillin, clindamycin, ciprofloxacin, rifampicin, tetracycline, and gentamicin were 91.2%, 87.3%, 79.3%, 56.3%, 45.2%, and 39.6%, respectively. All 126 strains were susceptible to vancomycin and linezolid. Conclusion: Non-diphtheriae Corynebacterium strains isolated from blood cultures of hospitalized patients with bacteremia may have multidrug resistance and the ability to produce biofilm. These results emphasize the importance of identifying strains and determining their antimicrobial susceptibility and biofilm production potential.

scholarly journals C-3 Substituted Lawsonemonoximates of Holmium(III): Synthesis, Characterization, and Antimicrobial Activity

2000 ◽  
Vol 7 (3) ◽  
pp. 147-150 ◽  
Author(s):  
S. B. Jagtap ◽  
S. G. Joshi ◽  
G. M. Litake ◽  
V. S. Ghole ◽  
B. A. Kulkarni
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Mueller Hinton ◽  
Providencia Stuartii ◽  
Derivatives Of ◽  
Klebsiella Spp

A series of five new metal complexes of Ho(III) with C-3 substituted derivatives of lawsonemonexime (2-hydroxy-1,4-naphthalenediene-1-oxime) were synthesized. The compounds were characterized by melting point, elemental analysis, IR spectroscopy and magnetic susceptibility. The antimicrobial activity of the compounds were determined by disk diffusion method and broth micro-dilution techniques using Mueller Hinton medium against the following organisms: S. aureus ATCC 6538P, Klebsiella pneumoniae, NCTC 418, Pseudomonas aeruginosa ATCC 27833, Salmonella typhimurium ATCC 23564, E. coli U 1777, E. coli HB101, Proteus morganii NCIM 2860, Providencia stuartii NCIM 2799 and Acinetobacter baumannii U 24. The chelates of Ho(III) with lawsonemonoxime and Ho(III) with 3-bromolawsonemonoxime showed a variable antimicrobial activity against all organisms tested except Pseudomonas and Klebsiella spp. S. aureus was found more sensitive to all ligands and chelates tested; but the MIC values of chelates were considerably less; thus having more antimicrobial effect.

scholarly journals Comparison of Activity of 10 Antibiotics Against Clinical Strains ofHelicobacter pyloriby Three Different Techniques

1998 ◽  
Vol 12 (3) ◽  
pp. 181-185 ◽  
Author(s):  
K Weiss ◽  
M Laverdiere ◽  
C Restieri
Keyword(s):  
Resistance Rate ◽  
Diffusion Method ◽  
Dilution Technique ◽  
Disk Diffusion Method ◽  
Clinical Strains ◽  
Metronidazole Resistance ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Agar Dilution ◽  
H Pylori

The authors determined the susceptibility of 55 single clinical strains ofHelicobacter pyloriisolated in the Montreal area to 10 antibiotics by three different methods - an agar dilution technique considered to be the gold standard, a disk diffusion method and the E-test. Testing was performed on Mueller-Hinton agar supplemented with 10% sheep blood; plates were incubated at 37°C for 72 h in a microaerophilic atmosphere. The metronidazole resistance rate is about 11% in the Montreal area. Macrolides are very active againstH pyloriisolates, with few variations in activity between older and newer molecules. Correlation among different methods was not as good as reported in the literature for metronidazole.

scholarly journals ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM LEAVES OF PISTACIA INTEGERRIMA (ANACARDIACEAE) STEW EX BRAND

2020 ◽  
pp. 90-92
Author(s):  
HEM RAJ VASHIST ◽  
SHIVANI DOGRA
Keyword(s):  
Antimicrobial Activity ◽  
Herbal Remedy ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Coagulase Negative Staphylococcus ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method ◽  
E Coli

Purpose: The ethanol extract of leaves of Pistacia integerrima (EEPI) was evaluated for antibacterial activity. Methods: The leaves of P. integerrima were collected from wild source and authenticated by Dr. Manoj Joshi, botanist environmental education expert, Una, HP, India. Antimicrobial activity was performed using the Mueller–Hinton (MH) agar using disk diffusion method. In the MH agar media 38 g after dissolving in 1000 ml of distilled water (pH 7.3±0.2), the extract was incorporated such that concentration per ml will be 66.67 μg, 133.33 and, 166.66. Results: The minimum inhibitory concentration recorded was 133.33 for Pseudomonas aeruginosa, Salmonella Typhi, and Klebsiella pneumonia, whereas 166.6 for Staphylococcus aureus, Coagulase-negative Staphylococcus, and Escherichia coli. The maximum zone of inhibition was found 19 mm and 18 mm for S. aureus and E. coli, respectively. S. aureus have been reported for skin pneumonia, heart valve, and bone infections whereas E. coli causes diarrhea and many other infections in children. Conclusion: After reporting the better zone of inhibition for these two bacteria, EEPI can be used to formulate better herbal remedy against them.

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