Ribonuclease H Enzyme Activity Detection Based on Hybridization Chain Reaction Amplification and Graphene Oxide Nanosheets Fluorescence Quenching
Ribonuclease H (RNase H) plays key roles in HIV virus replication process and can be used as an important target for anti-AIDS drugs screening. Therefore, we constructed a fluorescent nanosensor based on signal amplification of hybridization chain reaction (HCR) and fluorescence quenching of graphene oxide (GO) nanosheets. The nanosensor provided highly sensitive and selective platform for RNase H activity detection. This method also exhibited a good linear relationship in the range of 0.001–5 U/mL, and the detection limit was 0.0007 U/mL. Our results suggested that the developed system is a promising platform for monitoring the RNase H activity, showing great potential in the biomedical studies and drugs screening.
Increased efficacy of antileishmanial antisense phosphorothioate oligonucleotides in Leishmania amazonensis overexpressing ribonuclease H11Abbreviations: RNase H, ribonuclease H; S-oligo, phosphorothioate-modified oligodeoxyribonucleotide; LUAS, anti-luciferase antisense S-oligo; LUS, S-oligo complementary to LUAS; ASM, S-oligo antisense to Leishmania miniexon; SSM, S-oligo complementary to ASM; HIFBS, heat-inactivated fetal bovine serum; TbRH1, Trypanosoma brucei RNase H1 gene; RACE, rapid amplification of cDNA ends; MBSA, maleylated bovine serum albumin; ODN, oligodeoxyribonucleotide; PCR, polymerase chain reaction; and DMPC, dimyristoyl phosphatidylcholine.