The Clinical Significance of the Antinuclear Antibody Test as a Screening Procedure for DNA Antibodies in SLE

1980 ◽  
Vol 25 (4) ◽  
pp. 293-298 ◽  
Author(s):  
A. E. El-Ghobarey ◽  
D. J. P. Sloane ◽  
K. Whaley
Keyword(s):  
Dna Binding ◽  
Clinical Significance ◽  
Antinuclear Antibody ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Screening Procedure ◽  
Dna Antibodies ◽  
Screening Procedures

During the two-year period (1st October 1974–30th September 1976), 2979 sera were tested for DNA antibodies by the Farr test. One thousand six hundred and ninety-five of these were tested because they had high ANA titres (1/256 or greater). In this group 285 sera were found to have raised DNA binding capacities (DNA-bc), 86 of which were found in patients having diagnoses other than SLE. When the diagnoses were reviewed following the finding of a raised DNA-bc, 55 of these patients were found to be suffering from SLE. Of the 1284 sera tested for DNA antibodies without the prior ANA screening procedures, 288 were positive, 36 of which came from patients not considered to have SLE; 30 of these patients were subsequently shown to have SLE. Thus the DNA-bc test is an important tool in the diagnos is of SLE, and the ANA test appears to be a valuable screening procedure. The level of DNA-bc was not of any diagnostic value.

DETEKSI ANTIBODI MULTIPEL HEPATITIS C DALAM DARAH DONOR

2016 ◽  
Vol 21 (3) ◽  
pp. 261
Author(s):  
Ranti Permatasari ◽  
Aryati Aryati ◽  
Budi Arifah
Keyword(s):  
Hepatitis C ◽  
Predictive Value ◽  
Core Protein ◽  
Rapid Test ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Hcv Infection ◽  
Antibody Detection ◽  
Hcv Rna ◽  
Hcv Antibody

Hepatitis C (HCV) infection could be spread by blood transfusion. Screening of HCV in donor blood could prevent HCV infection to the recipient. HCV antibody test using rapid test of multiple antibody detection by immunochromatography method is an easy and rapid test that could detect four HCV antibodies separately. The aim of this study was to evaluate the diagnostic value of antibody HCV using multiple antibody detection rapid test in diagnosing HCV infection. This was an analytical observational study with a cross sectional design. The samples consisted of 42 donors’ blood serum from the Surabaya Branch of the Indonesian Red Cross which underwent HCV infection test using ELISA method. The samples were then tested using PCR HCV RNA as the gold standard and antibody HCV multiple antibodydetection rapid test The diagnostic value of HCV antibody test using multiple antibody detection rapid test by immunochromatography method showed a diagnostic sensitivity of 100%, diagnostic specificity of 75%, positive predictive value of 66.7% and negative predictive value of 100%, a diagnostic efficiency of 83.3%, with a positive probability ratio of 4 times. The most often positive antibody pattern was four (4) positive antibodies (core protein, NS3, NS4 and NS5). Core protein (CP) and NS3 were the most often positive antibodies. Based on this study result, the HCV antibody test using multiple antibody detection rapid test by immunochromatography method has a good diagnostic value.

scholarly journals Molecular characteristics of antibodies bearing an anti-DNA-associated idiotype.

1991 ◽  
Vol 174 (6) ◽  
pp. 1639-1652 ◽  
Author(s):  
A Manheimer-Lory ◽  
J B Katz ◽  
M Pillinger ◽  
C Ghossein ◽  
A Smith ◽  
...  
Keyword(s):  
B Cells ◽  
Dna Binding ◽  
Lupus Erythematosus ◽  
Germ Line ◽  
High Titer ◽  
Variable Region ◽  
Amino Acid Sequences ◽  
Molecular Characteristics ◽  
Systemic Lupus ◽  
Dna Antibodies

Anti-double-stranded DNA antibodies are the hallmark of the disease systemic lupus erythematosus and are believed to contribute to pathogenesis. While a large number of anti-DNA antibodies from mice with lupus-like syndromes have been characterized and their variable region genes sequenced, few human anti-DNA antibodies have been reported. We describe here the variable region gene sequences of eight antibodies produced by Epstein-Barr virus (EBV)-transformed B cells that bear the 3I idiotype, an idiotype expressed on anti-DNA antibodies and present in high titer in patients with systemic lupus. The comparison of these antibodies to the light chains of 3I+ myeloma proteins and serum antibodies reveals that EBV transformation yields B cells producing antibodies representative of the expressed antibody repertoire. The analysis of nucleotide and amino acid sequences of these antibodies suggests the first complementarity determining region of the light chain may be important in DNA binding and that paradigms previously generated to account for DNA binding require modification. The understanding of the molecular genetics of the anti-DNA response requires a more complete description of the immunoglobulin germ line repertoire, but data reported here suggest that somatic diversification is a characteristic of the anti-DNA response.

The Antinuclear Antibody Test

1981 ◽  
Vol 70 (3) ◽  
pp. 197-198
Author(s):  
Helene Paxton
Keyword(s):  
Antinuclear Antibody ◽  
Antibody Test

When to order an antinuclear antibody test

BMJ ◽  
2013 ◽  
Vol 347 (aug21 2) ◽  
pp. f5060-f5060 ◽  
Author(s):  
A. Binder ◽  
S. Ellis
Keyword(s):  
Antinuclear Antibody ◽  
Antibody Test
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