Serum β-glucuronidase activity as a biomarker for acute cholinesterase inhibitor pesticide poisoning

2018 ◽  
Vol 34 (12) ◽  
pp. 891-897 ◽  
Author(s):  
Doha M Beltagy ◽  
Kadry M Sadek ◽  
Amal S Hafez
Keyword(s):  
Serum Levels ◽  
Significant Negative Correlation ◽  
Repeated Measurements ◽  
Control Group ◽  
Pesticide Poisoning ◽  
Time Points ◽  
Diagnosis And Prognosis ◽  
Glucuronidase Activity ◽  
Reliable Marker ◽  
Group Serum

β-glucuronidase (BG) activity is a promising biomarker for diagnosis and prognosis after exposure to organophosphorous (OP) pesticides. The aim of this study was to evaluate the changes in serum BG activity in patients with acute OP poisoning and to determine whether these changes correlate with the severity of poisoning. Thirty patients with anticholinesterase pesticide poisoning were included, besides 10 healthy volunteers as a control group. Serum activities of butyrylcholinesterase (BuChE) and BG were measured for each subject on admission, then after 12 and 24 h. Serum levels of BuChE and BG in poisoned patients were significantly different from the control subjects; these differences persisted in repeated measurements. Moreover, the serum levels showed significant differences within each group of the three time points. A significant negative correlation was found between the serum activities of BuChE and BG in all groups at the three time points. In conclusion, serum BG activity seems a reliable marker for OP poisoning even when measured at 24 h after poisoning.

scholarly journals Are serum levels of 25-hydroxy vitamin D reduced following orchiectomy in testicular cancer patients?

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Klaus-Peter Dieckmann ◽  
Osama Andura ◽  
Uwe Pichlmeier ◽  
Klaus Martin Otte ◽  
Hendrik Isbarn ◽  
...  
Keyword(s):  
Vitamin D ◽  
Active Form ◽  
Germ Cell Tumors ◽  
Serum Levels ◽  
Repeated Measurements ◽  
Control Group ◽  
Testicular Germ Cell ◽  
Serum 25Ohd ◽  
Time Points ◽  
Urologic Diseases

Abstract Background The testis represents one place where the progenitor of vitamin D is converted into its active form. Loss of one testis was suggested to result in reduced vitamin D serum levels. Vitamin D deficiency would represent a significant health problem in the long-term course of patients with testicular germ cell tumors (GCTs) since most of them survive. The purpose of this study was to look to the serum 25(OH)-Vitamin D (25OHD) levels in patients with GCTs before and after orchiectomy. A total of 177 GCT patients underwent measurements of serum 25OHD levels, thereof 83 with preoperative measurements and 94 with measurements at six particular time-points from immediate postoperatively to >24 months. Longitudinal assessments of 25OHD serum levels were performed in individual patients with repeated measurements. A second analysis involved patient cohorts with measurements at six postoperative time-points. Serum levels of patients were also compared with 2 control groups, one consisting of 84 patients with non-neoplastic testicular diseases and another with 237 patients with non-neoplastic urologic diseases. We also looked to associations of 25OHD levels with levels of testosterone, follicle stimulating hormone (FSH), age, histology of GCT and season. Descriptive statistical methods were employed to compare groups and to analyze changes over time. Results Normal serum levels of 25OHD were found in 21.7%, 23.1%, 20.2%, 21.9% in GCT patients preoperatively, after >2 years, in control group 1 and control group 2, respectively. Levels were significantly higher in spring and summer, but no association was found with other parameters. We found a significant transient decrease of 25OHD levels with a nadir at 6-12 months after orchiectomy and a recovery thereafter. Conclusion Contrasting with previous studies we found no permanent reduction of serum 25OHD levels after orchiectomy but transient postoperative drop of 25OHD levels. There were no associations of 25OHD levels with age, and levels of testosterone or FSH. Our results may point to a particular role of the testis in vitamin D metabolism and may thus enhance the understanding of the diverse physiological roles of the testis.

scholarly journals Effects of Chronic Kidney Disease on levels of Oxidative Stress and Trace Elements

2021 ◽  
Author(s):  
Xiaojing Zhu ◽  
Yuan Zhang ◽  
Jin Han ◽  
Dan Xiao ◽  
Dan Zhu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Kidney Disease ◽  
Trace Elements ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Obvious Effect ◽  
The Relationship ◽  
Group Serum ◽  
Lower Expression

Abstract Introduction: The relationship between trace elements and OS in CKD patients is still not completely elucidated. The aim of this work is to determine the serum levels of OS and the trace elements in CKD patients.Methods: A total of 91 patients were enrolled and then divided into 3 groups at the basis of CKD stages. Healthy volunteers were included as a control group. Serum levels of SOD, MDA, Zn, Cu, Ca, Fe, and Mg were determined , and the correlation among these datas were analyzed.Results: The MDA level in the patients’ sera was significantly higher than the controls’ (P<0.05); however, their sera level of SOD was obviously lower (P<0.05). Meanwhile, the creatinine level in CKD patients’ sera was positively correlated with the MDA level (r = 0.534, P<0.01), while negatively with the expression of SOD (r = -0.427, P < 0.01). A lower expression of Zn and Ca values could be seen in the cases’ sera (P<0.05). Besides that, it showed a significant negative correlation between serum levels of MDA and Ca (r=-0.282, P<0.01), MDA and Zn (r=-0.358, P<0.01), while a positive correlation between MDA and Cu (r = 0.236, P=0.022). Adversely, the expression of SOD in the sera of cases was positively related to the levels of Ca (r=0.273, P<0.01) and Zn (r=0.277, P<0.01).Conclusion: Abnormalities of OS and Zn、Ca existed in CKD patients; and elevated OS may play a role in the trace elements imbalance. Dialysis seems to have no obvious effect on plasma trace elements.

Study on mechanism of Tim-3 on immune escape in benzene-induced acute myeloid leukemia

2020 ◽  
Author(s):  
qiong Ning ◽  
xiangxin li ◽  
Xiangdong Jian ◽  
Xiaopeng He
Keyword(s):  
Bone Marrow ◽  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Immune Escape ◽  
Serum Levels ◽  
Control Group ◽  
M2 Macrophages ◽  
Experimental Group ◽  
Group Serum ◽  
Acute Myeloid

Abstract To study the mechanism of Tim-3 on immune escape in benzene-induced acute myeloid leukemia (AML), to provide potential targets of clinical monitoring and intervention of hematological toxicity in benzene-induced AML . C3H/He mice were randomly divided into control group and experimental group. Serum levels of IL-12 in the experimental group were significantly lower than that in the control group. Serum levels of TGF-β1 in the experimental group were significantly higher than that in the control group( p <0.05). The proportion of Tim-3 positive CD14 + monocytes of bone marrow and spleen in the experimental group were both significantly higher than that in the control group ( p <0.05) by Flow cytometry (FCM). Compared with the control group, the expression of Tim-3 on (M1+M2) macrophages of bone marrow in the experimental group significantly increased by immunofluorescence assay. The expression of type M2 macrophages in (M1+M2) macrophages of bone marrow and spleen tissues in the experimental group were both higher than that in the control group. The expression levels of p-PI3K, p-AKT and p-mTOR in the experimental group were all significantly higher than that in the control group. Tim-3 was highly expressed in macrophages in benzene-induced AML. It promoted the activation of PI3K/AKT/mTOR signaling pathway, stimulated the secretion of anti-inflammatory cytokines, and inhibited the secretion of pro-inflammatory cytokines. High expression of Tim-3 changed the phenotype and function of macrophages by promoting the macrophages polarization, thus inducing negative immune response in the tumor microenvironment and tumor immune escape.

scholarly journals Metabonomic Responses of Grazing Yak to Different Concentrate Supplementations in Cold Season

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1595
Author(s):  
Jia Zhou ◽  
Shuangming Yue ◽  
Quanhui Peng ◽  
Lizhi Wang ◽  
Zhisheng Wang ◽  
...  
Keyword(s):  
Plasma Concentrations ◽  
Serum Levels ◽  
Rapeseed Meal ◽  
Cold Season ◽  
Supplementary Feeding ◽  
Control Group ◽  
Serum Metabolites ◽  
Treatment Groups ◽  
Group Serum ◽  
Highland Barley

Supplementation plays an important role in reversing the weight loss of grazing yaks during cold season. However, little is known about the effect of supplementation on the serum metabolites of grazing yaks. The objective of this study was to explore the effects of supplementary feeding on average daily gain (ADG) and serum metabolites with nuclear magnetic resonance (NMR)-based metabolomics method in growing yaks during cold season on the Qinghai-Tibetan plateau. Twenty 1.5-year-old female yaks (91.38 ± 10.43 kg LW) were evenly divided into three treatment groups and a control group (CON) (n = 5 per group). All the yaks were released to graze during daytime, whereas the yaks in the treatment groups were supplemented with highland barley (HLB), rapeseed meal (RSM), and highland barley plus rapeseed meal (HLB + RSM) at night. The whole experiment lasted for 120 days. Results indicated that the ADG of growing yak heifers was increased by concentrate supplementations, and ADG under HLB and HLB + RSM group was 37.5% higher (p < 0.05) than that with RSM supplementation. Supplementary feeding increased the plasma concentrations of total protein (TP), albumin (ALB), and blood urea nitrogen (BUN) of those in the CON group, and concentrations of BUN were higher in the RSM group than in the HLB and HLB + RSM group. Compared with the CON group, serum levels of glutamine, glycine, β-glucose were lower and that of choline was higher in the HLB group; serum levels of lactate were lower and that of choline, glutamate were higher in the HLB + RSM group. Compared with the HLB + RSM group, serum levels of glycerophosphoryl choline (GPC) and lactate were higher, and those of choline, glutamine, glutamate, leucine, N-acetyaspartate, α-glucose, and β-glucose were lower in the HLB group; serum levels of citrate, GPC and lactate were higher, and those of 3-Hydroxybutyrate, betaine, choline, glutamate, glutamine, N-acetylglycoprotein, N-acetyaspartate, α-glucose, and β-glucose were lower in the RSM group. It could be concluded that concentrate supplementations significantly improved the growth performance of growing yaks and supplementation with HBL or HLB plus RSM was better than RSM during the cold season. Supplementation with HBL or HLB plus RSM affected the serum metabolites of grazing yaks, and both treatments promoted lipid synthesis. Supplementation of yaks with HBL plus RSM could improve energy-supply efficiency, protein and lipid deposition compared with HLB and RSM.

scholarly journals Association of Adipocytokines with Lipid and Glycemic Profiles in Women with Normal Weight Obesity

2020 ◽  
Author(s):  
Ehsaneh Taheri ◽  
Saeed Hosseini ◽  
Mostafa Qorbani ◽  
Parvin Mirmiran
Keyword(s):  
Serum Level ◽  
Serum Levels ◽  
Normal Weight ◽  
Lipid Profiles ◽  
Anthropometric Measurement ◽  
Control Group ◽  
Model Assessment ◽  
Cardiometabolic Disorders ◽  
Normal Weight Obesity ◽  
Group Serum

Abstract Background: Subjects with normal weight obesity (NWO) are supposed to cardiometabolic disorders. The aim of this study was to investigate the circulating levels of vaspin and leptin and their association with glycemic and lipid profiles in women with NWO compared to controlsMethods: Forty women with body mass index (BMI)=18.5-24.9 kg/m2 and fat mass (FM) ≥ 30% as NWO group and 30 age matched women with same BMI range and FM<30% as control group were enrolled in this study. Anthropometric measurement, fasting serum levels of blood sugar (FBS), insulin, Glycated hemoglobin (HbA1c), lipid profiles and also, leptin and vaspin were measured.Results: The mean ± standard deviation (SD) of age was 28.76 ± 4.76 years in NWO group and 29.23 ± 4.50 years in controls. Subjects in NWO group had higher serum levels of insulin (9.02 ± 4.75 vs.6.24 ± 2.51, p= 0.009), leptin (17.31 ± 8.10 vs. 9.94 ± 4.30, p<0.001) and homeostatic model assessment for insulin resistance (HOMA-IR) (33.77 ± 20.71 vs. 23.48 ± 10.03, p=0.009) as compared to normal weight non-obese (NWNO) as control group. Serum level of vaspin in NWO group (34.82 pg/ml) was higher than that in controls (27.72 pg/ml), (p=0.12). In NWO group, serum level of leptin was correlated positively with FBS (r=0.45, p=0.02), insulin (r=0.51, p=0.008), and HOMA-IR (r=0.46, p=0.02) and vaspin concentration was positively associated with insulin (r=0.36, p= 0.02) and HOMA-IR (r=0.30, p=0.06). Conclusion: We observed that concentration of insulin and HOMA-IR index were significantly increased in women with NWO compared to the controls. Higher levels of leptin and vaspin in NWO were associated with glycemic profiles in NWO group.

scholarly journals Higher Circulating Concentration of Interleukin-38 in Patients with Knee Osteoarthritis: Its Association with Disease Severity

2021 ◽  
Author(s):  
Mitra Abassifard ◽  
Hossein Khorramdelazad ◽  
Shayan Rezaee ◽  
Abdollah Jafarzadeh
Keyword(s):  
Inflammatory Responses ◽  
Severe Disease ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Adaptive Immune ◽  
Valuable Marker ◽  
Vas Scores ◽  
Blood Specimens ◽  
Group Serum

Evidence showed that chronic inflammatory and immunopathological responses play a pivotal role in the development of osteoarthritis (OA). Interleukin-38 (IL-38) as a novel antiinflammatory cytokine with influential modulatory properties on both innate and adaptive immune responses can be involved in the pathogenesis of OA. Therefore, this study aimed to measure the serum level of IL-38 in OA patients to clarify the positive or negative association with disease and its severity. Blood specimens were collected from two groups including 23 newly-diagnosed OA patients and 22 healthy sex and age-matched subjects as a control group. Serum IL-38 quantities were measured using enzyme-linked immunosorbent assay (ELISA). Significantly higher IL-38 levels were detected in OA patients in comparison with the healthy group (265.78±41.27 pg/mL vs 44.23±6.04 pg/mL, p=0.0001). The IL-38 concentration in OA patients with Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores>40 and in OA patients with visual analog scale (VAS) scores>5 were higher than those with WOMAC scores<40, and VAS scores<5 (p=0.026 and p=0.035, respectively). The IL-38 levels in OA patients with body mass index (BMI)<25 were also significantly higher than in patients with BMI>25 (p=0.05). According to our findings, WOMAC, VAS, and BMI indices may influence the IL-38 serum levels in OA patients and it may be elevated in OA patients to modulate inflammatory responses in a compensatory manner. The patients with OA, especially those with more severe disease express higher serum amounts of IL-38. Accordingly, IL-38 may be considered as a valuable marker for OA.

scholarly journals A Novel Paradigm Based on ST2 and Its Contribution towards a Multimarker Approach in the Diagnosis and Prognosis of Heart Failure: A Prospective Study during the Pandemic Storm

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1080
Author(s):  
Radu-Stefan Miftode ◽  
Daniela Constantinescu ◽  
Corina Maria Cianga ◽  
Antoniu Octavian Petris ◽  
Amalia-Stefana Timpau ◽  
...  
Keyword(s):  
Heart Failure ◽  
Area Under The Curve ◽  
Serum Levels ◽  
Diagnostic Value ◽  
Control Group ◽  
Diagnosis And Prognosis ◽  
Test Kit ◽  
Positive Correlations ◽  
Lactate Levels ◽  
A Prospective Study

Background: Acute heart failure (HF) represents an increasingly common and challenging presentation in the emergency room, also inducing a great socio-economic burden. Extensive research was conducted toward finding an ideal biomarker of acute HF, both in terms of sensitivity and specificity, but today practicians’ interest has shifted towards a more realistic multimarker approach. Natriuretic peptides (NPs) currently represent the gold standard for diagnosing HF in routine clinical practice, but novel molecules, such as sST2, emerge as potentially useful biomarkers, providing additional diagnostic and prognostic value. Methods: We conducted a prospective, single-center study that included 120 patients with acute HF and 53 controls with chronic HF. Of these, 13 patients (eight with acute HF, five from the control group) associated the coronavirus-19 disease (COVID-19). The diagnosis of HF was confirmed by a complete clinical, biological and echocardiographic approach. Results: The serum levels of all studied biomarkers (sST2, NT-proBNP, cardiac troponin) were significantly higher in the group with acute HF. By area under the curve (AUC) analysis, we noticed that NT-proBNP (AUC: 0.976) still had the best diagnostic performance, closely followed by sST2 (AUC: 0.889). However, sST2 was a significantly better predictor of fatal events, showing positive correlations for both in-hospital and at 1-month mortality rates. Moreover, sST2 was also associated with other markers of poor prognosis, such as the use of inotropes or high lactate levels, but not with left ventricle ejection fraction, age, body mass index or mean arterial pressure. sST2 levels were higher in patients with a positive history of COVID-19 as compared with non-COVID-19 patients, but the differences were statistically significant only within the control group. Bivariate regression showed a positive and linear relationship between NT-proBNP and sST2 (r(120) = 0.20, p < 0.002). Conclusions: we consider that sST2 has certain qualities worth integrating in a future multimarker test kit alongside traditional biomarkers, as it provides similar diagnostic value as NT-proBNP, but is emerging as a more valuable prognostic factor, with a better predictive value of fatal events in patients with acute HF.

scholarly journals Association of Adipocytokines with Lipid and Glycemic Profiles in Women with Normal Weight Obesity

2020 ◽  
Author(s):  
Ehsaneh Taheri ◽  
Saeed Hosseini ◽  
Mostafa Qorbani
Keyword(s):  
Serum Level ◽  
Positive Association ◽  
Serum Levels ◽  
Normal Weight ◽  
Lipid Profiles ◽  
Anthropometric Measurement ◽  
Control Group ◽  
Cardiometabolic Disorders ◽  
Normal Weight Obesity ◽  
Group Serum

Abstract Background: Subjects with normal weight obesity (NOW) are supposed to cardiometabolic disorders. The aim of this study was to investigate the circulating levels of vaspin and leptin and their association with glycemic and lipid profiles in women with NWO compared to controlsMaterial & Methods: Forty women with BMI:18.5-24.9 kg/m2 and FM ≥ 30% as a NOW group and 30 age matched women with same BMI range and FM<30% as control group were enrolled. Anthropometric measurement, fasting serum levels of fasting blood sugar (FBS), insulin, HbA1c, lipid profiles and also, leptin and vaspin were measured. Results: The mean ± SD of age were 28.76±4.76 years in NWO group and 29.23 ± 4.50 years in controls. Subjects in NWO group had higher serum level of insulin (9.02 ± 4.75 vs.6.24 ± 2.51, p= 0.009), leptin (17.31 ± 8.10 vs. 9.94 ± 4.30, p<0.001) and HOMA-IR (33.77 ± 20.71 vs. 23.48 ± 10.03, p=0.009) compared to the NWNO group. Serum level of vaspin was higher in NWO (34.82 pg/ml) than control group (27.72 pg/ml),(p=0.12). In NWO subjects, serum level of leptin showed positive association with FBS (r=0.45, p=0.02), insulin (r=0.51, p=0.008), and HOMA-IR (r=0.46, p= 0.02) and vaspin concentration was positively associated with insulin (r=0.36, p= 0.02) and HOMA-IR (r=0.30, p=0.06). Conclusion: We observed that women with NWO had statistically significant increased concentration of insulin and HOMA-IR index compared to the controls. Higher levels of leptin and vaspin in NWO were associated with glycemic profiles in NWO.

scholarly journals The role of serum 25-hydroxyvitamin D, ionized calcium and phosphorus in breast cancer females: A case-control study

2015 ◽  
Vol 4 (2) ◽  
pp. 228 ◽  
Author(s):  
Nadia Abd El Moneim ◽  
Taha Hewala ◽  
Mona Rashad ◽  
Yousria Moussa ◽  
Nehal Abu-Samra ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Serum Levels ◽  
Ionized Calcium ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Serum Ionized Calcium ◽  
Group Serum

<p><strong>Aim:</strong> To evaluate the diagnostic and prognostic value of serum 25- hydroxyvitamin D (25(OH) D), ionized calcium and phosphorus in comparison with serum CA15.3 as the most commonly used breast cancer marker.</p><p><strong>Participants and methods:</strong> This study was conducted on 45 breast cancer female patients with recently detected breast cancer before surgery and 45 apparently healthy female controls of matched age, menstrual and socioeconomic status as breast cancer patients group. Serum 25(OH) D, ionized calcium, phosphorus and CA15.3 were measured using ready-for-use commercially available kits.</p><p><strong>Results:</strong> Serum levels of 25(OH) D and ionized calcium in the breast cancer patients group were significantly lower than those of the control group, while serum levels of phosphorus and CA15.3 in breast cancer patients group were significantly higher than those of the control group. The area under the ROC curve for serum ionized calcium (81.7%) was significantly greater than that of 25(OH) D (75.3%), CA 15.3 (70.1%) and phosphorus (62.8%). The odd’s ratio of vitamin D was 0.0937 (95% CI=0.0311-0.2823), of ionized calcium was 0.0464 (95% CI=0.015-0.141) and of phosphorus was 2.6801(95% CI=1.1269- 6.3742) in breast cancer patients group. Serum phosphorus was significantly correlated with age and menopausal status of breast cancer patients.</p><p><strong>Conclusion:</strong> Our results suggest that serum ionized calcium and 25(OH) D were superior to serum CA15.3 and phosphorus for prediction of breast cancer. In addition, our results indicate that 25 (OH) D and calcium may decrease the risk for breast cancer incidence, while phosphorus may increase this risk. None of the assayed biomarkers has a prognostic role in breast cancer.</p>

AMH levels in girls with various pubertal problems

2017 ◽  
Vol 30 (3) ◽  
Author(s):  
Senay Savas-Erdeve ◽  
Elif Sagsak ◽  
Meliksah Keskin ◽  
Semra Cetinkaya ◽  
Zehra Aycan
Keyword(s):  
Standard Deviation Score ◽  
Central Precocious Puberty ◽  
Primordial Follicle ◽  
Free Testosterone ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Sex Hormone Binding Globulin ◽  
Control Group ◽  
Premature Thelarche ◽  
And Control

AbstractBackground:Anti-Müllerian hormone (AMH) is produced by granulosa cells surrounding follicles that have undergone recruitment from the primordial follicle pool but have not been selected for dominance (preantral and early antral follicles). In healthy girls, serum levels of AMH vary considerably between individuals. We aimed to evaluate the AMH level in girls with premature thelarche (PT) and central precocious puberty (CPP).Methods:Girls with CPP (n=21), PT (n=24) and a control prepubertal group (n=22) were included in the study.Results:AMH levels were significantly higher in the PT group than the prepubertal control group and similar to the CPP group. AMH levels in the CPP group were similar to the prepubertal control group. AMH levels showed a significant negative correlation with luteinizing hormone (LH), free testosterone and dehydroepiandrosterone sulphate (DHEAS) levels in the PT group. AMH levels were negatively correlated with height standard deviation score (HSDS), body mass index (BMI) SDS values and positively correlated with sex hormone binding globulin (SHBG) levels in the CPP group. These levels were positively correlated with SHBG levels in the control prepubertal group.Conclusions:Serum AMH levels in girls with PT was found to be higher than in prepubertal girls. AMH levels in the CPP group were not different compared with the PT and control groups.

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