scholarly journals UVB irradiation of human platelet concentrates does not prevent HLA alloimmunization in recipients

Blood ◽  
1994 ◽  
Vol 84 (10) ◽  
pp. 3524-3531
Author(s):  
MA Grijzenhout ◽  
MI Aarts-Riemens ◽  
FR de Gruijl ◽  
H van Weelden ◽  
HC van Prooijen
Keyword(s):  
Cell Death ◽  
Hla Antigens ◽  
Ultraviolet B ◽  
Control Group ◽  
Unexpected Finding ◽  
Uvb Radiation ◽  
Platelet Concentrates ◽  
Uvb Irradiation ◽  
Hla Class I Antigens ◽  
Delayed Cell Death

Exposure of platelet concentrates (PCs) to ultraviolet B radiation (UVB) has been advocated as an alternative method for prevention of the onset of HLA sensitization in recipients. In this study, pooled PCs were irradiated in a Haemonetics UV irradiator (Haemonetics Corp, Braintree, MA) at a dose that did not induce platelet activation. The effect of UVB irradiation on prevention of primary HLA sensitization was evaluated in a prospective controlled clinical study performed in cardiac patients undergoing cardiopulmonary bypass. Patients were treated with filtered red blood cells and a single transfusion of either standard (control group) or UVB-irradiated (UVB group) pooled platelets prepared from 12 donors. Five of 39 patients in the control group and 6 of 62 patients in the UVB group developed allo-antibodies against HLA antigens, which is not significantly different (P = .62). This unexpected finding prompted us to check the efficacy of UVB irradiation. We determined UVB-specific DNA damage in cells by measuring the fluorescence from a labeled specific monoclonal antibody against thymine dimers. With this novel flow cytometer technique, we estimated in UVB-irradiated leukocytes in saline that a mean fluorescence intensity (MFI) of 47 +/- 2 arbitrary units (n = 6) correlated with abolition of alloreactivity in mixed lymphocyte cultures and delayed cell death (within 72 hours). MFI in leukocytes suspended in plasma and exposed to the clinical dose of UVB was sixfold higher (310 +/- 41 arbitrary units) and resulted in early cell death (within 24 hours). We hypothesize that this high level of UVB radiation induces fragmentation of the leukocytes. As a consequence, the poor results of UVB irradiation may be explained by the onset of HLA- alloimmunization induced by soluble donor HLA class I antigens processed and presented by host antigen-presenting cells.

scholarly journals UVB irradiation of human platelet concentrates does not prevent HLA alloimmunization in recipients

Blood ◽  
1994 ◽  
Vol 84 (10) ◽  
pp. 3524-3531 ◽  
Author(s):  
MA Grijzenhout ◽  
MI Aarts-Riemens ◽  
FR de Gruijl ◽  
H van Weelden ◽  
HC van Prooijen
Keyword(s):  
Cell Death ◽  
Hla Antigens ◽  
Ultraviolet B ◽  
Control Group ◽  
Unexpected Finding ◽  
Uvb Radiation ◽  
Platelet Concentrates ◽  
Uvb Irradiation ◽  
Hla Class I Antigens ◽  
Delayed Cell Death

Abstract Exposure of platelet concentrates (PCs) to ultraviolet B radiation (UVB) has been advocated as an alternative method for prevention of the onset of HLA sensitization in recipients. In this study, pooled PCs were irradiated in a Haemonetics UV irradiator (Haemonetics Corp, Braintree, MA) at a dose that did not induce platelet activation. The effect of UVB irradiation on prevention of primary HLA sensitization was evaluated in a prospective controlled clinical study performed in cardiac patients undergoing cardiopulmonary bypass. Patients were treated with filtered red blood cells and a single transfusion of either standard (control group) or UVB-irradiated (UVB group) pooled platelets prepared from 12 donors. Five of 39 patients in the control group and 6 of 62 patients in the UVB group developed allo-antibodies against HLA antigens, which is not significantly different (P = .62). This unexpected finding prompted us to check the efficacy of UVB irradiation. We determined UVB-specific DNA damage in cells by measuring the fluorescence from a labeled specific monoclonal antibody against thymine dimers. With this novel flow cytometer technique, we estimated in UVB-irradiated leukocytes in saline that a mean fluorescence intensity (MFI) of 47 +/- 2 arbitrary units (n = 6) correlated with abolition of alloreactivity in mixed lymphocyte cultures and delayed cell death (within 72 hours). MFI in leukocytes suspended in plasma and exposed to the clinical dose of UVB was sixfold higher (310 +/- 41 arbitrary units) and resulted in early cell death (within 24 hours). We hypothesize that this high level of UVB radiation induces fragmentation of the leukocytes. As a consequence, the poor results of UVB irradiation may be explained by the onset of HLA- alloimmunization induced by soluble donor HLA class I antigens processed and presented by host antigen-presenting cells.

scholarly journals Protective Effect of Bixin Isolated from Bixa orellana L. Seeds on UVB-Induced Inflammation and Immunosuppression of the Skin

2018 ◽  
Vol 18 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Atina Hussaana ◽  
Suparmi ◽  
Hani Afnita Murti
Keyword(s):  
Medical Science ◽  
Skinfold Thickness ◽  
Ultraviolet B ◽  
Contact Hypersensitivity ◽  
Control Group ◽  
Uvb Radiation ◽  
Bixa Orellana ◽  
Uvb Irradiation ◽  
Significant Difference ◽  
Inflammatory Edema

Background: DNA damage caused by excessive ultraviolet B (UVB) radiation on the skin triggers the response to inflammatory and immunosuppression. The bixin from Bixa orellana L. has been proven to be able to inhibit cyclo-oxygenase. Objective: to verify whether the bixin lotion has the effect to offer protection against inflammation and immunosuppression due to acute UVB irradiation in shaved BALB /c mice. Methods: Protection against inflammation and immunosuppression, respectively were studied in 4 groups of mice. Each group was treated respectively with topical application of base lotion as a control; bixin lotion doses of 0.5 mg; 2.5 mg and 125 mg, for 10 days prior to and during the UVB irradiation. The Inflammation was induced by UVB irradiation, 360 mJ/cm2 once a day for 3 consecutive days, whereas the immunosuppression was induced by UVB irradiation, 360 mJ/cm2 once a day for 5 consecutive days. The inflammatory response was measured as an increase in middorsal skinfold thickness at the peak response. The immune response was measured as the contact hypersensitivity (CHS) response to oxasolon sensitization. Results: The results indicated that in concentration range used, bixin lotion significantly decreased the middorsal skinfold thickness at 72 hours after UVB radiation (p <0.05) compared to the control, but there was no significant difference between couples of the dose of bixin. Bixin lotion was also capable to restore the suppression of CHS from 34.22% in the control group to 11.4%; 0.5% and 0% at doses of 0.5; 2.5 and 125 mg respectively (p <0.05). Conclusion: Bixin lotion has the potential to reduce the inflammatory edema reaction and the suppression of CHS of mice induced by UVB radiation. Bangladesh Journal of Medical Science Vol.18(1) 2019 p.107-111

scholarly journals Protective Effect of Spirulina-Derived C-Phycocyanin against Ultraviolet B-Induced Damage in HaCaT Cells

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 273
Author(s):  
Young Ah Jang ◽  
Bo Ae Kim
Keyword(s):  
Antioxidant Defense System ◽  
Skin Aging ◽  
Ultraviolet B ◽  
Control Group ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Blue Green Algae ◽  
Radiation Group ◽  
Skin Wrinkles

Background and objectives: Reactive oxygen species (ROS) overwhelm the antioxidant defense system, induce oxidative stress, and increase matrix metalloproteinase (MMP) expression, resulting in skin aging. Thus, preventing ultraviolet B (UVB)-induced skin damage can attenuate skin aging. Spirulina (a biomass of cyanobacteria, also called blue-green algae) is comprised of prokaryotes, whereas microalgae are eukaryotes and are rich in phycocyanin, a powerful antioxidant. Materials and Methods: Here, we investigated the photoprotective effects of spirulina-derived C-phycocyanin (C-PC) against UVB radiation using keratinocytes (HaCaT cells). Results: UVB radiation increased MMP-1 and MMP-9 expression but decreased involucrin, filaggrin, and loricrin expression. C-PC showed no toxicity at concentrations of 5–80 μg/mL in terms of HaCaT cell viability. UVB-irradiated HaCaT cells had a 50.8% survival rate, which increased to 80.3% with C-PC treatment. MMP expression increased with UVB treatment, whereas MMP-1 and MMP-9 concentrations decreased with C-PC treatment. UVB reduced involucrin, filaggrin, and loricrin expression in HaCaT cells, but 80 μg/mL C-PC increased their expression by >25%. In the UVB radiation group, dichlorofluorescin diacetate fluorescence intensity in HaCaT cells increased by 81.6% compared with that in the control group, whereas ROS production was reduced by 51.2% and 55.1% upon treatment with 40 and 80 μg/mL C-PC, respectively. Conclusions: C-PC might reduce or prevent skin aging by reducing UVB irradiation-induced skin wrinkles and free radicals.

In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVB-treated platelet concentrates

Transfusion ◽  
2015 ◽  
Vol 55 (9) ◽  
pp. 2219-2230 ◽  
Author(s):  
Mélanie Abonnenc ◽  
Giona Sonego ◽  
David Crettaz ◽  
Alessandro Aliotta ◽  
Michel Prudent ◽  
...  
Keyword(s):  
Platelet Function ◽  
In Vitro Study ◽  
Ultraviolet B ◽  
Vitro Study ◽  
Uvb Radiation ◽  
Platelet Concentrates

Leukocyte Apoptosis and Inflammation in Iron-Overloaded Patients with Sickle Cell Disease or β-Thalassemia: A Mechanism for Increased Stroke and Disease Severity in Sickle Cell Disease.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1233-1233 ◽  
Author(s):  
Patrick B. Walter ◽  
David W. Killilea ◽  
Ellen B. Fung ◽  
Annie Lui ◽  
Jacqueline Madden ◽  
...  
Keyword(s):  
Cell Death ◽  
Sickle Cell Disease ◽  
Disease Severity ◽  
Sickle Cell ◽  
Strong Predictor ◽  
Acute Chest Syndrome ◽  
Control Group ◽  
Cell Disease ◽  
Delayed Cell Death ◽  
Leukocyte Number

Abstract Sickle cell disease (SCD) is a hemoglobinopathy characterized by micro-vascular hypoxia-reperfusion, inflammation and leukocytosis. Studies in SCD have shown that leukocytosis is a strong predictor of stroke and disease severity. It is known that leukocytosis and inflammation contribute to increased leukocyte-endothelial adhesion and vasoocclusive events. Leukocytosis or increased leukocyte number is determined by the balance between cell death programs (apoptosis) and proliferation. In this study, we examine markers of apoptosis and proliferation in SCD as compared to thalassemia (a hemoglobinopathy that is vasculitis negative) and a control group. Methods: Markers of leukocyte turnover, inflammation and free iron (NTBI, non-transferrin bound iron) were compared in 11 patients with SCD (7M, 13 ± 4 yrs), 18 with thalassemia (7M, 24 ± 9 yrs) and 10 disease-free controls (5M, 27 ± 12 yrs). All SCD and thalassemia patients were healthy and event free in the previous 4 months. Blood was obtained fasting and prior to RBC transfusion; and plasma, serum and cells were separated by centrifugation. The pro-apoptotic markers nucleosome protein (DNA laddering) and Bax (initiator of mitochondrial permeability) and inflammatory marker, high-sensitivity C Reactive Protein (hsCRP) were determined by ELISA. NTBI (DNA damage inducing, pro-apoptotic) was measured by HPLC. Plasma levels of cytokines (anti-apoptotic, proliferation stimulating) were determined by using a multiplex bead-based immunoassay. Plasma lactate dehydrogenase (LDH), a marker of hemolysis, was also measured because hemolysis products have been shown to inhibit apoptosis. Results: Leukocyte number and absolute neutrophil count were 1.8 and 2.2 fold higher in SCD compared to thalassemia (P&lt;0.01). Evidence for decreased apoptosis in SCD included reduced nuleosome protein as compared to thalassemia and reduced Bax as compared to controls. In addition, LDH was significantly increased 1.9 fold in SCD relative to thalassemia (p=0.012). In contrast, NTBI was 2 fold higher in thalassemia than SCD and correlated with nucleosome protein (R=0.45; p=0.013). Finally, high levels of the cytokines IL-6 and IL-10 in SCD relative to thalassemia may be stimulating leukocyte proliferation and survival. These cytokines and hsCRP are also positively correlated with leukocyte number. Conclusions: These preliminary findings demonstrate that the balance of apoptosis vs. proliferation favors delayed cell death and enhanced cell proliferation in SCD, leading to increased circulating leukocytes. These changes may be related to hemolysis, increased levels of inflammatory cytokines, and lower amounts of NTBI in SCD. This leukocytosis increases the potential for vasoocclusive events that contribute to stroke, acute chest syndrome or renal tubule damage.

scholarly journals Mirabilis Jalapa L. Protein as Inductor of Apoptosis on UVB-induced Skin Damage in Mice

2017 ◽  
Vol 16 (3) ◽  
pp. 423-427
Author(s):  
Atina Hussaana ◽  
Sismindari ◽  
Sitarina Widyarini ◽  
Sudjadi ◽  
Zullies Ikawati
Keyword(s):  
Protective Effect ◽  
Dna Fragmentation ◽  
Caspase 3 ◽  
Control Group ◽  
Peak Time ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Uvb Irradiation ◽  
L Protein ◽  
Mirabilis Jalapa

Background: Mirabilis jalapa L. protein (MJ-Protein) has been shown to have antioxidant and anti-inflammatory effects in vitro. Thus, it has a potential protective effect against ultraviolet B (UVB)-induced skin damage.Objective: To determine the protective effect and mechanism of MJ protein in UVB-radiation exposed mouse skin.Methods: In this experimentalstudy, 30 female BALB/c mice aged 6 weeks were exposed to a single dose of UVB irradiation with 3 minimal erythema doses (MEDs) and continued with the treatment of 0.6 mg MJ-Protein topically. The number of apoptotic body (sunburn cells) formed in epidermal layers of mouse dorsal skin was assessed at 1, 24, 48, 72, 96 and 120h after UVB irradiation was compared to that of the control group. The difference in the sunburn cells number between two groups were analyzed using independent T-test with the level of significance of 0.05. The apoptosis mechanism was confirmed qualitatively by caspase-3 and DNA fragmentation analysis in vitro.Results:At 24 h after the UVB exposure (peak time for sunburn cells formation), there was a significant increase in the sunburn cells number in the group treated with topical application of MJ-Protein. There was increased caspase-3 expression and DNA fragmentation in HeLa cells treated with MJ-Protein.Conclusions: MJ-Protein protects againts UVB-induced skin damage in mice trough apoptosis induction.Bangladesh Journal of Medical Science Vol.16(3) 2017 p.423-427

scholarly journals Protective effect of total flavonoids from boxthorn leaf against UVB irradiation-induced skin injury

2021 ◽  
Vol 18 (9) ◽  
pp. 1943-1947
Author(s):  
Qing Yu ◽  
Ying Shen ◽  
Yadan Gan ◽  
Liang Zheng
Keyword(s):  
Protective Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Ultraviolet B ◽  
Control Group ◽  
Total Flavonoids ◽  
Skin Damage ◽  
Skin Injury ◽  
Uvb Irradiation ◽  
Base Group

Purpose: To investigate the protective effect of total flavonoids from boxthorn leaf against skin injury induced by UVB irradiation, and to elucidate the underlying mechanism of action. Method: Healthy female mice (n = 100) were randomly divided into four groups: normal control group, UV negative control group, cream base group, and boxthorn leaf total flavonoid (BLTF) group, with 25 mice in each group. The mice in each group were irradiated with ultraviolet B (UVB) irradiation instrument for 1.5 h daily for 3 weeks. Mice in the cream base group were smeared with cream base on their backs, while mice in BLTF group were smeared with 15 mg/g boxthorn BLTF cream. The control and negative control group mice were not treated. Changes in superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels were determined using standard methods. Results: Compared to the negative control group, the levels of SOD and GSH-Px in the control group and BLTF were significantly elevated, while MDA levels declined significantly (p < 0.05). Although higher GSH-Px and SOD levels, and lower MDA were seen in the cream base group than in negative control group, these indices were comparable for the two groups (p > 0.05). Conclusion: The total flavonoids of boxthorn leaves improve resistance to UVB-induced skin damage by regulating SOD, MDA and GSH-Px levels in the skin of mice. Thus, they exert protective effects on the skin.

scholarly journals Effect of Lactobacillus reuteri Administration on Wrinkle Formation and Type I Procollagen Levels in UVB-Exposed Male Balb/c Mice (Mus musculus)

2020 ◽  
Vol 4 (3) ◽  
pp. 113
Author(s):  
Ivanna Valentina ◽  
Achadiyani Achadiyani ◽  
Sunarjati Sudigdo Adi ◽  
Ronny Lesmana ◽  
Reni Farenia
Keyword(s):  
Oxidative Stress ◽  
Lactobacillus Reuteri ◽  
Ultraviolet B ◽  
Type I ◽  
Uvb Radiation ◽  
Dorsal Skin ◽  
Uvb Irradiation ◽  
Type I Procollagen ◽  
Group 3 ◽  
Group 1

Background: Chronic Ultraviolet B (UVB) exposure causes oxidative stress that may induce damages to the collagen matrix and thus plays a role in the wrinkle formation. Lactobacillus reuteri is a probiotic that may exerts antioxidant effects, thus helping to reduce damages caused by UVB-induced oxidative stress in the skin.Materials and Methods: Twenty-eight male Balb/c mice were divided equally into control group, UVB radiation only group, oral L. reuteri supplementation only group, and UVB radiation with oral L. reuteri supplementation group. UVB irradiation was given 3 times a week (100 seconds/exposure, within 3 cm distance) for 10 weeks, with a total dose of 166 mJ/cm2. Oral L. reuteri supplementation (0.2 mL, 108 CFU) was provided every morning after meal via orogastric feeding tube for 10 weeks. Wrinkle formation on the dorsal skin of the mice was evaluated in accordance with the Bissett method and type I procollagen levels was evaluated by western blotting.Results: In comparison with the group receiving only UVB irradiation, the group receiving probiotic and UVB irradiation showed significantly lower wrinkle scores (Group 1 vs. Group 3, 2.50±0.55 vs. 1.00±0,00; p<0.05) and significantly higher type I procollagen levels (Group 1 vs. Group 3, 0.88±0.36 vs. 1.92±0.46; p<0.05).Conclusion: Results of the current study showed that L. reuteri supplementation may reduce wrinkle formation and increase type I procollagen production in UVB-exposed dorsal skin of male Balb/c mice.Keywords: Lactobacillus reuteri, type I procollagen, photoaging, wrinkles, ultraviolet B

scholarly journals Dermal Mast Cells Determine Susceptibility to Ultraviolet B–induced Systemic Suppression of Contact Hypersensitivity Responses in Mice

1998 ◽  
Vol 187 (12) ◽  
pp. 2045-2053 ◽  
Author(s):  
Prue H. Hart ◽  
Michele A. Grimbaldeston ◽  
Georgina J. Swift ◽  
Aleksandra Jaksic ◽  
Frances P. Noonan ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Ultraviolet B ◽  
Contact Hypersensitivity ◽  
Delayed Type Hypersensitivity ◽  
Parental Origin ◽  
Uvb Radiation ◽  
Uvb Irradiation ◽  
Systemic Immunosuppression ◽  
Different Strains

Different strains of mice have varying susceptibilities to ultraviolet radiation (UV) of wavelength 280–320 nm (UVB) for 50% suppression of systemic contact hypersensitivity (CHS) responses. Prevalence of histamine-staining dermal mast cells in different strains of mice (C57BL/ 6J, DBA/2, BALB/c) correlated directly with their susceptibility to UVB-induced systemic immunosuppression. BALB/c mice carrying Uvs1, a major locus for susceptibility to UV-induced immunosuppression, contained greater numbers of dermal mast cells than BALB/c mice of the same parental origin. Strains of mice that were differentiated on their susceptibility to UVB-induced downregulation of systemic CHS responses were similar in their susceptibility to histamine-induced immunomodulation. Histamine, but not UVB irradiation, decreased systemic CHS responses in mast cell–depleted mice (W  f/W  f). Reconstitution of the dorsal skin of W  f/W  f mice with bone marrow–derived mast cell precursors from nonmutant mice rendered the mice susceptible to UVB irradiation for systemic suppression of CHS responses. UVB irradiation did not suppress delayed type hypersensitivity responses to allogeneic spleen cells in W  f/W  f mice. In contrast, UV irradiation suppressed CHS responses in W  f/W  f mice when hapten was applied to the irradiated site. This study demonstrates that dermal mast cells are necessary for the induction of systemic suppression of CHS responses by UVB radiation, and suggests that mast cell– derived histamine is one component of this UVB-induced systemic immunosuppression.

scholarly journals Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1

2021 ◽  
Vol 13 (1) ◽  
pp. 84-90
Author(s):  
Dimpuulina Erna Mariati ◽  
Sunarjati Sudigdoadi ◽  
Ronny Lesmana ◽  
Astrid Feinisa Khairani ◽  
Julia Windi Gunadi ◽  
...  
Keyword(s):  
Ultraviolet B ◽  
Control Group ◽  
Coffee Bean ◽  
Uvb Radiation ◽  
Epidermal Thickness ◽  
Robusta Coffee ◽  
Matrix Metalloproteinase 1 ◽  
Before And After ◽  
Expression Evaluation ◽  
Skin Samples

BACKGROUND: Recently, coffee is widely used for preventing photoaging because of its antioxidant capacity. Among two kinds of coffee, robusta coffee has higher content of antioxidant such as chlorogenic acid (CGA) and caffeine. Researchs about robusta coffee bean effect on photoaging due to UVB radiation is still limited. Therefore, the aim of this study was to examine the effect of robusta extract cream (RE cream) on preventing wrinkle in mice induced by ultraviolet-B (UVB) radiation.METHODS: RE cream was made by mixing RE coffee with moisturizing cream in different concentration (10%, 20%, and 40%). Twenty-five male of Mus musculus Balb/c strain mice aged 4 weeks were divided into five groups; control group, UVB group, UVB + 10% RE group, UVB + 20% RE group, and UVB + 40% RE group. The UVB groups were given UVB radiation three times a week with an exposure duration of 100 seconds per time for ten weeks. At the end of the treatment, skin samples were excised and statined histologically, also were analyzed for their protein expression. Evaluation of wrinkles was carried out using the Bissete method before and after treatment. To evaluate the thickness of the epidermis, HE staining was performed, while masson Trichome staining was performed to determine the collagen content.RESULTS: RE cream-treated groups showed lower wrinkle score compared to the control group. Furthermore, in UVB + 10% RE group, the RE cream application reduce wrinkle formation. In UVB + 10% RE group and UVB + 20% RE group, the RE cream application increased epidermal thickness and collagen content (p=0.00). While collagenase, matrix metalloproteinase-1 (MMP-1) expression was lower in UVB + 20% RE group compared to the UVB group (p<0.05), however the MMP1 expression in UVB + 40% RE group was higher than other treatment group.CONCLUSION: RE cream prevents wrinkle by maintaining epidermal thickness and collagen contain. RE cream also decreases MMP-1 expression in mice.KEYWORDS: coffee, collagen, MMP-1, robusta, wrinkle

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