scholarly journals Ontogeny of RORγt+ cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiuhao Zhao ◽  
Wenhua Liang ◽  
Yonghui Wang ◽  
Ruirong Yi ◽  
Lingjie Luo ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Necrotizing Enterocolitis ◽  
Premature Infants ◽  
Immune Cells ◽  
Early Life ◽  
Pathological Process ◽  
Newborn Mice ◽  
Intestinal Lamina Propria ◽  
Specific Antagonist ◽  
Lamina Propria Lymphocytes

Abstract Background Neonates possess an immature and plastic immune system, which is a major cause of some diseases in newborns. Necrotizing enterocolitis (NEC) is a severe and devastating intestinal disease that typically affects premature infants. However, the development of intestinal immune cells in neonates and their roles in the pathological process of NEC have not been elucidated. Results We examined the ontogeny of intestinal lamina propria lymphocytes in the early life of mice and found a high percentage of RORγt+ cells (containing inflammatory Th17 and ILC3 populations) during the first week of life. Importantly, the proportion of RORγt+ cells of intestinal lamina propria further increased in both NEC mice and patients tissue than the control. Furthermore, the application of GSK805, a specific antagonist of RORγt, inhibited IL-17A release and ameliorated NEC severity. Conclusions Our data reveal the high proportion of RORγt+ cells in newborn mice may directly contribute to the development of NEC.

Effects of somatostatin on human intestinal lamina propria lymphocytes. Modulation of lymphocyte activation

1991 ◽  
Vol 31 (3) ◽  
pp. 211-219 ◽  
Author(s):  
S. Fais ◽  
B. Annibale ◽  
M. Boirivant ◽  
A. Santoro ◽  
F. Pallone ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Lymphocyte Activation ◽  
Intestinal Lamina Propria ◽  
Lamina Propria Lymphocytes

scholarly journals Human intestinal lamina propria lymphocytes are naturally permissive to HIV-1 infection

1999 ◽  
Vol 29 (4) ◽  
pp. 1202-1208 ◽  
Author(s):  
Caterina Lapenta ◽  
Monica Boirivant ◽  
Marco Marini ◽  
Stefano M. Santini ◽  
Mariantonia Logozzi ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Intestinal Lamina Propria ◽  
Lamina Propria Lymphocytes ◽  
Hiv 1

scholarly journals A103 PROBIOTIC FORMULATIONS DIFFERENTIALLY AFFECT DISEASE OUTCOME IN A MOUSE MODEL OF NECROTIZING ENTEROCOLITIS

2020 ◽  
Vol 3 (Supplement_1) ◽  
pp. 119-120
Author(s):  
S Robinson ◽  
H Zhu ◽  
Z Zhang ◽  
B Li ◽  
A Pierro ◽  
...  
Keyword(s):  
Body Weight ◽  
Necrotizing Enterocolitis ◽  
Premature Infants ◽  
Tissue Damage ◽  
Gastrointestinal Disease ◽  
Health Benefit ◽  
Disease Outcome ◽  
Newborn Mice ◽  
Probiotic Intervention ◽  
Over Time

Abstract Background Necrotizing enterocolitis (NEC) causes severe gastrointestinal disease in premature infants. A precise etiology is not yet known, but it is generally agreed that intestinal immaturity & alterations in the gut microbiota play key roles in NEC progression. There is growing interest in the clinical use of probiotics for the prevention of NEC in at-risk infants. Some probiotics, which are defined as beneficial bioactive microbes that provide a health benefit when administered in sufficient amounts, may influence disease outcome in premature infants with NEC. Aims To investigate the efficacy of two commercially available probiotic formulations, Lacidofil® and ProBiokid®, in the prevention of experimentally-induced NEC. Methods At gestational day 12.5, treated dams were administered probiotics (109 cfu/mL) in drinking water (Table 1). NEC was induced at postnatal day 5 or when pups were ≥ 3.0 g by the administration of hyperosmolar formula, hypoxia, and lipopolysaccharide (4 mg/kg). NEC pups receiving probiotics were administered 108cfu in hyperosmolar formula once daily during the morning feeding. Body weight, survival and disease severity were scored. Results Neither probiotic intervention affected overall survival of NEC-induced pups. Control dam-fed pups (NN) continued to gain weight as expected over time, while untreated NEC pups (NNNEC) exhibited a significant delay in weight gain. LLNEC pups exhibited the greatest percent body weight increase over time, possibly due to a lower birth weight. Lacidofil administration did not improve NEC-induced tissue damage irrespective of the timing of intervention. In contrast, Probiokid provided to both dam and pups (PPNEC) resulted in less severe tissue damage compared to their control counterparts. Conclusions These finding demonstrate strain-specific differences in two different commercial probiotic formulations in the prevention of NEC in newborn mice. Future studies seek to provide insight into how Lacidofil and Probiokid engage intestinal stem cells (Lgr5-GFP mice), cell proliferation (Ki67 positivity) and differentiation (MUC2 & Chromogranin A positivity) in the NEC-damaged intestine. Funding Agencies CIHRMITACS Accelerate Postdoctoral Award

scholarly journals In vitro immunoglobulin synthesis by human intestinal lamina propria lymphocytes.

Gut ◽  
1984 ◽  
Vol 25 (6) ◽  
pp. 649-655 ◽  
Author(s):  
P A Drew ◽  
J T La Brooy ◽  
D J Shearman
Keyword(s):  
Lamina Propria ◽  
Intestinal Lamina Propria ◽  
Immunoglobulin Synthesis ◽  
Lamina Propria Lymphocytes
Sign in / Sign up

Export Citation Format

Share Document