Lack of effect of ageing on acetate oxidation in rat skeletal muscle during starvation: a (13)C NMR study

2000 ◽  
Vol 203 (6) ◽  
pp. 995-1001
Author(s):  
V. Mezzarobba ◽  
G. Bielicki ◽  
F.M. Jeffrey ◽  
M. Mignon ◽  
J.P. Renou ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Citrate Synthase ◽  
Acetate Oxidation ◽  
Rat Skeletal Muscle ◽  
Acetyl Coenzyme A ◽  
Nmr Study ◽  
Old Rats ◽  
C Nmr

Acetate oxidation was examined by (13)C nuclear magnetic resonance in skeletal muscle from adult and old rats. Rats fasted for 5 days were perfused with [2-(13)C]acetate over 2 h, and muscle extracts were analyzed for [(13)C]glutamate isotopomers. This study shows that approximately 80 % of acetyl-coenzyme A entering the tricarboxylic cycle was derived from substrate infusion in both adult and old rats, and that the flux through anaplerotic pathways was approximately 21 % of the flux through citrate synthase. These data demonstrate that skeletal muscle from adult and old rats oxidizes the same proportion of exogenous acetate.

Oxidation of lactate and acetate in rat skeletal muscle: analysis by 13C-nuclear magnetic resonance spectroscopy

1997 ◽  
Vol 83 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Loren A. Bertocci ◽  
John G. Jones ◽  
Craig R. Malloy ◽  
Ronald G. Victor ◽  
Gail D. Thomas
Keyword(s):  
Skeletal Muscle ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Skeletal Muscles ◽  
Resonance Spectroscopy ◽  
Rat Skeletal Muscle ◽  
Isotopomer Analysis ◽  
C Nmr ◽  
Nuclear Magnetic

Bertocci, Loren A., John G. Jones, Craig R. Malloy, Ronald G. Victor, and Gail D. Thomas. Oxidation of lactate and acetate in rat skeletal muscle: analysis by13C-nuclear magnetic resonance spectroscopy. J. Appl. Physiol. 83(1): 32–39, 1997.—The balance between carbohydrate and fatty acid utilization in skeletal muscle previously has been studied in vivo by using a variety of methods such as arteriovenous concentration differences and radioactive isotope tracer techniques. However, these methodologies provide only indirect estimates of substrate oxidation. We used 13C-nuclear magnetic resonance (NMR) spectroscopy and non-steady-state isotopomer analysis to directly quantify the relative oxidation of two competing exogenous substrates in rat skeletal muscles. We infused [1,2-13C]acetate and [3-13C]lactate intravenously in anesthetized rats during the final 30 min of 35 ( n = 10) or 95 ( n = 10) min of intense, unilateral, rhythmic hindlimb contractions.13C-NMR spectroscopy and isotopomer analysis were performed on extracts of gastrocnemius and soleus muscles from both the contracting and contralateral resting hindlimbs. We found that 1) [13C]lactate and [13C]acetate were taken up and oxidized by both resting and contracting skeletal muscles; and 2) high-intensity muscle contractions altered the pattern of substrate utilization such that the relative oxidation of acetate decreased while that of lactate remained unchanged or increased. Based on these findings, we propose that13C-NMR spectroscopy in combination with isotopomer analysis can be used to study the general dynamics of substrate competition between carbohydrates and fats in rat skeletal muscle.

scholarly journals A13C NMR study of the interactions of Ag13CN and Ag(CN)2–with thiomalic acid, L-methionine and DL-selenomethionine

2005 ◽  
Vol 19 (5,6) ◽  
pp. 275-281 ◽  
Author(s):  
Anvarhusein A. Isab ◽  
Mohamed I. M. Wazeer
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Chelate Complexes ◽  
Neutral Ph ◽  
Nmr Study ◽  
Insoluble Polymer ◽  
C Nmr ◽  
Nuclear Magnetic

Complexation of Ag+as AgNO3, solid AgCN and Ag(CN)2–by labeled and unlabeled L-methionine, DL-selenomethionine and d,l-thiomalate were studied by nuclear magnetic resonance methods. The13C NMR indicates that only Ag+react with the both L-methionine, DL-selenomethionine at neutral and higher pH via CO2–and S or Se atom forming a chelate. The Ag(CN)2–and AgCN do not bind to either of these two ligands at any pH. The Ag13CN, which is an insoluble polymer, can react with thiomalate to form chelate complexes at neutral pH. Various structures for the chelate formations are proposed.

1H and 13C nuclear magnetic resonance study of the complexation of uranyl ion with malic acid

1982 ◽  
Vol 60 (8) ◽  
pp. 1007-1018 ◽  
Author(s):  
M. Teresa Nunes ◽  
Victor M. S. Gil ◽  
António V. Xavier
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Malic Acid ◽  
Nuclear Magnetic Resonance Study ◽  
Magnetic Resonance Study ◽  
Molar Ratios ◽  
Nmr Study ◽  
13C Nuclear Magnetic Resonance ◽  
Ph Range ◽  
C Nmr

A full pH range 1H and 13C nmr study was performed of the complexation of UO22+ with malic acid, for variable concentrations and molar ratios. Spectral evidence for the existence of at least five complexes was found, and their stoichiometry and dependence on pH were investigated. Information on the conformation of the bound ligand molecules was also obtained.

scholarly journals The FT-IR, FT-Raman,1H and13C NMR study on molecular structure of sodium(I), calcium(II), lanthanum(III) and thorium(IV) cinnamates

2010 ◽  
Vol 24 (3-4) ◽  
pp. 277-281 ◽  
Author(s):  
M. Kalinowska ◽  
W. Lewandowski ◽  
R. Swislocka ◽  
E. Regulska
Keyword(s):  
Molecular Structure ◽  
Nuclear Magnetic Resonance ◽  
Electronic Structure ◽  
Magnetic Resonance ◽  
Chemical Shifts ◽  
1H And13c Nmr ◽  
Nmr Study ◽  
Ft Ir ◽  
C Nmr ◽  
Ft Raman

In this work the effect of sodium(I), calcium(II), lanthanum(III) and thorium(IV) ions on the electronic structure of cinnamic acid (phenylacrylic acid) was studied. In this research: infrared (FT-IR), Raman (FT-Raman), nuclear magnetic resonance (1H,13C NMR) were used. In the series of Na(I)→ Ca(II)→ La(III)→ Th(IV) cinnamates: (1) systematic shifts of several bands in the FT-IR and FT-Raman spectra, and (2) regular chemical shifts of protons1H and13C nuclei were observed.

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