The unreliability of mammalian glomerular markers in teleostean renal studies

1976 ◽  
Vol 64 (2) ◽  
pp. 369-378 ◽  
Author(s):  
K. W. Beyenbach ◽  
L. B. Kirschner

1. The assumption that (3H) methoxy inulin, (14C) polyethylene glycol (PEG) and (125) iothalamate (glofil) are reliable volume and glomerular markers in teleosts was tested. 2. PEG occupied smaller volumes than inulin and glofil in sea-water-adapted Salmo gairdneri. Ureteral clearances of PEG were about 22% higher than those of inulin and glofil, and urine-to-plasma ratios were significantly greater for PEG than for the other two markers. 3. After introduction into the urinary bladder the three macro-molecules appeared in the plasma, PEG at the lowest rates. 4. These observations indicate that mammalian glomerular markers can penetrate the bladder and possibly the ureters and renal tubules. Therefore, their clearances may not give a true measure of glomerular filtration rates in teleosts.

1975 ◽  
Vol 229 (2) ◽  
pp. 389-393 ◽  
Author(s):  
KW Beyenbach ◽  
LB Kirschner

Magnesium concentration in the urinary bladder of marine teleosts is high and [Na] is low. The inverse relationship may be due to the coupling of Mg secretion to Na reabsorption through a common tubular transport system or coupling may be indirect. Unanesthetized sea-water-adapted Salmo gairdneri were infused with saline or MgCl2, and ureteral urine was collected. Over a wide range of Mg excretion rates, tubular Mg secretion and Na reabsorption show no clear correlation as might be expected from an obligatory tubular Mg/Na exchange. Instead ureteral Mg and Na concentrations and excretion rates are positively correlated. These data are not consistent with the presence of a tubular exchange system. When ureteral urine was allowed to remain in the urinary bladder before being analyzed the inverse relationship between [Mg] and [Na] developed because the bladder reabsorbed Na and H2O, thereby concentrating Mg. Hence, tubular Mg/Na exchanges do not produce the inverse Mg/Na relationship in bladder urine. Instead, Mg is secreted in the nephron, while Na and H2O are reabsorbed from the bladder.


1963 ◽  
Vol 40 (2) ◽  
pp. 335-341
Author(s):  
W. N. HOLMES ◽  
R. L. McBEAN

1. Variations in the glomerular filtration rate (G.F.R.) and the renal tubular reabsorption of water are probably important factors in the homoiostatic mechanisms associated with the euryhalinity of the rainbow trout (Salmo gairdneri). 2. The observed reduction in urine flow after the adaptation of rainbow trout to sea water can be largely accounted for on the basis of the reduced G.F.R. 3. Intraperitoneal injections of mammalian preparations of vasopressin, oxytocin and vasopressin/oxytocin into freshwater trout significantly increased the G.F.R. 4. The intraperitoneal injection of aldosterone into freshwater fish had no detectable effect on G.F.R. 5. The similar administration of corticosterone significantly reduced the G.F.R. to 73% of the intact freshwater control value.


Recent work has determined the depth of the Mohorovičić discontinuity at sea and has made it likely that peridotite xenoliths in basaltic volcanic rocks are samples of material from below the discontinuity. It is now possible to produce a hypothetical section showing the transition from a continent to an ocean. This section is consistent with both the seismic and gravity results. The possible reactions of the crust to changes in the total volume of sea water are dis­cussed. It seems possible that the oceans were shallower and the crust thinner in the Archean than they are now. If this were so, some features of the oldest rocks of Canada and Southern Rhodesia could be explained. Three processes are described that might lead to the formation of oceanic ridges; one of these involves tension, one compression and the other quiet tectonic conditions. It is likely that not all ridges are formed in the same way. It is possible that serpentization of olivine by water rising from the interior of the earth plays an important part in producing changes of level in the ocean floor and anomalies in heat flow. Finally, a method of reducing gravity observations at sea is discussed.


As is well known, the glomeruli of the frog’s kidney are supplied with blood only by the renal arteries, whereas the renal tubules have a double supply. On the one hand, they receive blood by way of the renal portal veins; on the other hand, the efferent vessels from the glomeruli open into the capillary network round the tubules. The whole of the tubule receives blood from each of these two sources, so that the capillary network around the tubules can be fully injected either from the renal arteries or from the renal portal veins. Taking advantage of this fact it has been shown by Beddard and one of us (F. A. B.) that after complete occlusion of the glomeruli the tubules, when adequately supplied with oxygen, maintain their normal histological appearance, and may secrete urine. In the present experiments an attempt has been made to determine the function of the glomerulus and to ascertain whether the tubules possess the capacity to absorb water and solids.


1974 ◽  
Vol 60 (1) ◽  
pp. 167-181
Author(s):  
J. A. RIEGEL ◽  
A. P. M. LOCKWOOD ◽  
J. R. W. NORFOLK ◽  
N. C. BULLEID ◽  
P. A. TAYLOR

1. Measurements have been made to determine the blood volume, bladder volume, clearance of 131I-sodium diatrizoate and U/H for diatrizoate in the crabs Carcinus maenas and Macropipus (Portunus) depurator. 2. Observed values of clearance blood volume and bladder volume in the two species at 18 °C were: Clearance (as % blood volume per day), Macropipus 56.1±14.5; Carcinus 27.1±5.8; Blood volume (as % body weight), Macropipus 21.0±4.0; Carcinus 19.2±3.0; Bladder volume (as % blood volume), Macropipus 12.1 ±5.0; Carcinus 11.0±8.0. 3. It is shown that the measured U/H differs from that to be expected if no reabsorption of water or secretion of diatrizoate occurs. 4. 14C-inulin and 51Cr-EDTA are excreted in an essentially similar manner to 131I-diatrizoate by Carcinus, implying that any active secretion of diatrizoate must be small in magnitude. 5. Injections of ethacrynic acid decrease the U/H ratio for diatrizoate relative to that in control Carcinus injected with sea water. In some Carcinus the concentration of diatrizoate in the urine comes to exceed that initially present in the blood. Both these points are taken, with 3, as support for the conclusion that water can be withdrawn from the primary urine of Carcinus.


1903 ◽  
Vol 3 (3-4) ◽  
pp. 154-155
Author(s):  
M. A. Vasiliev

Beneficial influence is so-called. sexual operations in persons with hypertrophy of prostatae was explained by the advancing atrophy of the last days. But the rapid onset of the result, on the one hand, and the negative data of the microscopic examination of the prostate after the operation, on the other, showed that the atrophy of the prostate was not very good here. In addition to the explanation of this fact, they put a decrease in the congestion of the urinary tract, which was considered an ethological moment for hypertrophy of the prostatae. smyavinoschago duct) on the tone of the sphincteris vesicae, in order to find out in this way partly the clinical significance of these operations.


1957 ◽  
Vol 188 (3) ◽  
pp. 477-484 ◽  
Author(s):  
Bodil Schmidt-Nielsen ◽  
Knut Schmidt-Nielsen ◽  
T. R. Houpt ◽  
S. A. Jarnum

The nitrogen excretion was studied in the one-humped camel, Camelus dromedarius. When a growing camel was maintained on a low N intake (dates and hay) the amount of N excreted in the form of urea, NH3 and creatinine decreased to 2–3 gm/day. This decrease was caused by a drop in urea excretion from 13 gm to 0.2–0.5 gm/day. Urea given intravenously during low N intake was not excreted but was retained. (The camel like other ruminants can utilize urea for microbial synthesis of protein.) The renal mechanism for urea excretion was investigated by measuring urea clearance and glomerular filtration rate during a period of 7 months. During normal N intake about 40% of the urea filtered in the glomeruli were excreted in the urine while during low N intake only 1–2% were excreted. The variations in urea clearance were independent of the plasma urea concentration and of glomerular filtration rate, but were related to N intake and rate of growth. No evidence of active tubular reabsorption of urea was found since the urine urea concentration at all times remained higher than the simultaneous plasma urea concentration. The findings are not in agreement with the current concept for the mechanism of urea excretion in mammals. It is concluded that the renal tubules must either vary their permeability to urea in a highly selective manner or secrete urea actively.


2021 ◽  
Vol 869 (1) ◽  
pp. 012056
Author(s):  
M Ramadhaniaty ◽  
C Octavina ◽  
FA Putri ◽  
S Karina ◽  
Ichsan

Abstract Crassostrea gigas is a Pacific oyster that has the largest size among the other oysters. This oyster is most often found because of its ability to adapt to a variety of environments. Krueng Cut is an estuary area that gets a lot of nutrient input from sea water because it is directly adjacent to the ocean. This condition makes this area has a high abundance of oysters with high fishing and antropogenic activities as well. The purpose of this study was to determine the density and distribution of the oyster population from 3 representative stations along the estuary area of Krueng Cut. The method used in this study is random sampling using a quadratic transect. The results show that the highest density is indicated by station 3 with 55.67 ind/m2 and the lowest density is indicated by station 1 is 40.33 ind/m2. These results indicate that the activities of residents, the entry of contaminants from antropogenic affect the density of oysters. The distribution of oysters from the three stations showed similarities, namely they tended to grouping. The aquatic environment at the three research stations still supports the growth of oysters. This study confirms that there has been a decline in the population, which is characterized by a decrease in the number of catches and a smaller size of oysters.


1835 ◽  
Vol 125 ◽  
pp. 355-358 ◽  

The Fourth Memoir, published in my Zoological Researches and Illustrations, No. III. page 69, &c., having first made known the real nature of the Cirripedes , the key of which remained concealed in their metamorphosis, it might have been expected that some naturalist favourably situated to investigate the oceanic tribe of these animals, would have been the first to make the same discovery in regard to these, and thereby complete their natural history. It was scarcely to be expected that the honour of this discovery also should be reserved for the author, fixed to one spot, where none of them naturally exist, and are but casually thrown upon our shores by the waves of the Atlantic, attached to pieces of wreck, or brought into port fixed to the bottoms of ships returning from distant voyages. Fortunately, however, two ships of this description came into this harbour (Cork), one from the Mediterranean, the other from North America, which, not being sheathed with copper, had their bot­toms literally covered with Barnacles of the three genera of Lepas , Cineras , and Otion ; and having persons employed expressly for the purpose, numbers of these were brought alive in sea water, amongst which were many with the ova in various stages of their progress, and some ready to hatch, which they eventually did in prodigious numbers, so as to enable him to add the proof of their being, like the Balani, natatory Crusta­cea in their first stage , but of a totally different facies and structure; a circumstance which determines the propriety of the separation of the Cirripedes into two tribes, and evinces the sagacity of Mr. MacLeay in being the first to indicate that these two tribes, the Balani and Lepades , were not so closely related as generally supposed. The larvæ of the Balani , described in Memoir IV. under the external appearance of the bivalve Monoculi ( Astracoda ), have a pair of pedunculated eyes, more numerous and more completely developed members, approximating to those of Cyclops , and of the perfect Triton ; while, in the present type, or Lepades , the larva resembles some­what that of the Cyclops , which Müller, mistaking for a perfect animal, named Amymone , and which can be shown to he common to a great many of the Entomostraca ; or the resemblance is still more striking to that of the Argulus Armiger of Latreille, which, in fact, is but an Amymone furnished with a tricuspidate shield at the back.


Author(s):  
M. R. Clarke ◽  
E. J. Denton ◽  
J. B. Gilpin-Brown

Squids (teuthoids) fall into two distinct groups according to their density in sea water. Squids of one group are considerably denser than sea water and must swim to stop sinking; squids in the other group are nearly neutrally buoyant. Analyses show that in almost all the neutrally buoyant squids large amounts of ammonium are present. This ammonium is not uniformly distributed throughout the body but is mostly confined to special tissues where its concentration can approach half molar. The locations of such tissues differ according to the species and developmental stage of the squid. It is clear that the ammonium-rich solution are almost isosmotic with sea water but of lower density and they are present in sufficient volume to provide the main buoyancy mechanism of these squids. A variety of evidence is given which suggests that squids in no less than 12 of the 26 families achieve near-neutral buoyancy in this way and that 14 families contain squids appreciably denser than sea water [at least one family contains both types of squid]. Some of the ammonium-rich squids are extremely abundant in the oceans.


Sign in / Sign up

Export Citation Format

Share Document