scholarly journals ROS Production via P2Y1-PKC-NOX2 Is Triggered by Extracellular ATP after Electrical Stimulation of Skeletal Muscle Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0129882 ◽  
Author(s):  
Alexis Díaz-Vegas ◽  
Cristian A. Campos ◽  
Ariel Contreras-Ferrat ◽  
Mariana Casas ◽  
Sonja Buvinic ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Cells ◽  
Extracellular Atp ◽  
Skeletal Muscle Cells ◽  
Ros Production ◽  
Stimulation Of

scholarly journals Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca2+ signals and an IL-6 autocrine loop

2014 ◽  
Vol 306 (8) ◽  
pp. E869-E882 ◽  
Author(s):  
Mario Bustamante ◽  
Rodrigo Fernández-Verdejo ◽  
Enrique Jaimovich ◽  
Sonja Buvinic
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Cells ◽  
Extracellular Atp ◽  
Negative Regulator ◽  
Skeletal Muscle Cells ◽  
Nucleotide Receptors ◽  
Calcium Signals ◽  
Autocrine Loop ◽  
Stat3 Phosphorylation

Interleukin-6 (IL-6) is an important myokine that is highly expressed in skeletal muscle cells upon exercise. We assessed IL-6 expression in response to electrical stimulation (ES) or extracellular ATP as a known mediator of the excitation-transcription mechanism in skeletal muscle. We examined whether the canonical signaling cascade downstream of IL-6 (IL-6/JAK2/STAT3) also responds to muscle cell excitation, concluding that IL-6 influences its own expression through a positive loop. Either ES or exogenous ATP (100 μM) increased both IL-6 expression and p-STAT3 levels in rat myotubes, a process inhibited by 100 μM suramin and 2 U/ml apyrase. ATP also evoked IL-6 expression in both isolated skeletal fibers and extracts derived from whole FDB muscles. ATP increased IL-6 release up to 10-fold. STAT3 activation evoked by ATP was abolished by the JAK2 inhibitor HBC. Blockade of secreted IL-6 with a neutralizing antibody or preincubation with the STAT3 inhibitor VIII reduced STAT3 activation evoked by extracellular ATP by 70%. Inhibitor VIII also reduced by 70% IL-6 expression evoked by ATP, suggesting a positive IL-6 loop. In addition, ATP increased up to 60% the protein levels of SOCS3, a negative regulator of the IL-6 signaling pathway. On the other hand, intracellular calcium chelation or blockade of IP3-dependent calcium signals abolished STAT3 phosphorylation evoked by either extracellular ATP or ES. These results suggest that expression of IL-6 in stimulated skeletal muscle cells is mediated by extracellular ATP and nucleotide receptors, involving IP3-dependent calcium signals as an early step that triggers a positive IL-6 autocrine loop.

Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production

2018 ◽  
Vol 26 (5) ◽  
pp. 1265-1272 ◽  
Author(s):  
Asie Sadeghi ◽  
Atefeh Rostamirad ◽  
Shadisadat Seyyedebrahimi ◽  
Reza Meshkani
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Ros Production

NFAT activation by membrane potential follows a calcium pathway distinct from other activity-related transcription factors in skeletal muscle cells

2008 ◽  
Vol 294 (3) ◽  
pp. C715-C725 ◽  
Author(s):  
Juan Antonio Valdés ◽  
Eduardo Gaggero ◽  
Jorge Hidalgo ◽  
Nancy Leal ◽  
Enrique Jaimovich ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Transcription Factors ◽  
Electrical Stimulation ◽  
Stimulus Frequency ◽  
Muscle Cells ◽  
C2c12 Cells ◽  
Skeletal Muscle Cells ◽  
Mrna Levels ◽  
Activated T Cells ◽  
Nfat Activation

Depolarization of skeletal muscle cells triggers intracellular Ca2+ signals mediated by ryanodine and inositol 1,4,5-trisphosphate (IP3) receptors. Previously, we have reported that K+-induced depolarization activates transcriptional regulators ERK, cAMP response element-binding protein, c- fos, c- jun, and egr-1 through IP3-dependent Ca2+ release, whereas NF-κB activation is elicited by both ryanodine and IP3 receptor-mediated Ca2+ signals. We have further shown that field stimulation with electrical pulses results in an NF-κB activation increase dependent of the amount of pulses and independent of their frequency. In this work, we report the results obtained for nuclear factor of activated T cells (NFAT)-mediated transcription and translocation generated by both K+ and electrical stimulation protocols in primary skeletal muscle cells and C2C12 cells. The Ca2+ source for NFAT activation is through release by ryanodine receptors and extracellular Ca2+ entry. We found this activation to be independent of the number of pulses within a physiological range of stimulus frequency and enhanced by long-lasting low-frequency stimulation. Therefore, activation of the NFAT signaling pathway differs from that of NF-κB and other transcription factors. Calcineurin enzyme activity correlated well with the relative activation of NFAT translocation and transcription using different stimulation protocols. Furthermore, both K+-induced depolarization and electrical stimulation increased mRNA levels of the type 1 IP3 receptor mediated by calcineurin activity, which suggests that depolarization may regulate IP3 receptor transcription. These results confirm the presence of at least two independent pathways for excitation-transcription coupling in skeletal muscle cells, both dependent on Ca2+ release and triggered by the same voltage sensor but activating different intracellular release channels.

scholarly journals Alignment of Skeletal Muscle Cells Cultured in Collagen Gel by Mechanical and Electrical Stimulation

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Takara Tanaka ◽  
Noriko Hattori-Aramaki ◽  
Ayano Sunohara ◽  
Keisuke Okabe ◽  
Yoshiaki Sakamoto ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Collagen Gel ◽  
Muscle Cells ◽  
Mechanical Force ◽  
C2c12 Cells ◽  
Skeletal Muscle Cells ◽  
Collagen Gels ◽  
Cells Cultured

For in vitro tissue engineering of skeletal muscle, alignment and fusion of the cultured skeletal muscle cells are required. Although the successful alignment of skeletal muscle cells cultured in collagen gel has been reported using a mechanical force, other means of aligning cultured skeletal muscle cells have not been described. However, skeletal muscle cells cultured in a two-dimensional dish have been reported to align in a uniform direction when electrically stimulated. The purpose of this study is to determine if skeletal muscle cells cultured three-dimensionally in collagen gels can be aligned by an electrical load. By adding direct current to cells of the C2C12 skeletal muscle cell line cultured in collagen gel, it was possible to align C2C12 cells in a similar direction. However, the ratio of alignment was better when mechanical force was used as the means of alignment. Thus for tissue engineering of skeletal muscle cells, electrical stimulation may be useful as a supplementary method.

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