scholarly journals Aquaporins are main contributors to root hydraulic conductivity in pearl millet [Pennisetum glaucum (L) R. Br.]

PLoS ONE ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. e0233481
Author(s):  
Alexandre Grondin ◽  
Pablo Affortit ◽  
Christine Tranchant-Dubreuil ◽  
Carla de la Fuente-Cantó ◽  
Cédric Mariac ◽  
...  
Keyword(s):  
Hydraulic Conductivity ◽  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Root Hydraulic Conductivity

scholarly journals Aquaporins are main contributors of root hydraulic conductivity in pearl millet [Pennisetum glaucum (L) R. Br.]

2020 ◽  
Author(s):  
Alexandre Grondin ◽  
Pablo Affortit ◽  
Christine Tranchant-Dubreuil ◽  
Carla de la Fuente Cantó ◽  
Cédric Mariac ◽  
...  
Keyword(s):  
Hydraulic Conductivity ◽  
Water Use Efficiency ◽  
Water Transport ◽  
Pearl Millet ◽  
Water Use ◽  
Pennisetum Glaucum ◽  
Root Hydraulic Conductivity ◽  
Plant Water Use ◽  
Whole Plant ◽  
Use Efficiency

AbstractPearl millet is a key cereal for food security in arid and semi-arid regions but its yield is increasingly threatened by water stress. Physiological mechanisms consisting in saving water or increasing water use efficiency can alleviate that stress. Aquaporins (AQP) are water channels contributing to plant hydraulic balance that are supposedly involved in these mechanisms by mediating root water transport. However, AQP remain largely uncharacterized in pearl millet. Here, we studied AQP function in root water transport in two pearl millet lines contrasting for water use efficiency (WUE). We observed that these lines were also contrasting for root hydraulic conductivity (Lpr) and AQP contribution to Lpr, the line with lower WUE showing significantly higher AQP contribution to Lpr. To investigate the AQP isoforms contributing to Lpr, we developed genomic approaches to first identify the entire AQP family in pearl millet and second study the plasma membrane intrinsic proteins (PIP) gene expression profile. We identified and annotated 33 AQP genes in pearl millet among which ten encoded PIP isoforms. PgPIP1-3 and PgPIP1-4 were significantly more expressed in the line showing lower WUE, higher Lpr and higher AQP contribution to Lpr. Overall, our study suggests that AQP from the PIP1 family are the main contributor of Lpr in pearl millet and are possibly associated to whole plant water use mechanisms. This study paves the way for further investigations on AQP functions in pearl millet hydraulics and adaptation to environmental stresses.The newly sequenced nucleotide sequences reported in this article have been submitted to GenBank under the submission number 2333840 (TPA grp467567). Assignment of GenBank accession number is in process.

Pearl millet (Pennisetum glaucum) contrasting for the transpiration response to vapour pressure deficit also differ in their dependence on the symplastic and apoplastic water transport pathways

2018 ◽  
Vol 45 (7) ◽  
pp. 719 ◽  
Author(s):  
Murugesan Tharanya ◽  
Kaliamoorthy Sivasakthi ◽  
Gloria Barzana ◽  
Jana Kholová ◽  
Thiyagarajan Thirunalasundari ◽  
...  
Keyword(s):  
Hydraulic Conductivity ◽  
Water Transport ◽  
Pearl Millet ◽  
Transpiration Rate ◽  
Vapour Pressure Deficit ◽  
Transport Pathways ◽  
Root Hydraulic Conductivity ◽  
Detached Leaves ◽  
Apoplastic Pathway ◽  
Symplastic Pathway

Genotypic differences in transpiration rate responses to high vapour pressure deficit (VPD) was earlier reported. Here we tested the hypothesis that this limitation could relate to different degrees of dependence on the apoplastic (spaces between cells), and symplastic water transport pathways (through cells via aquaporin-facilitated transport), which are known to have different hydraulic conductivities. The low transpiration rate (Tr) genotype PRLT 2/89/33 either restricted its transpiration under high VPD, or was more sensitive to VPD than H77/833-2, when grown hydroponically or in soil. The slope of the transpiration response to an ascending series of VPD was lower in whole plants than in de-rooted shoots. In addition, the transpiration response of detached leaves to moderately high VPD (2.67 kPa), normalised against leaves exposed to constant VPD (1.27 kPa), was similar in low and high Tr genotypes. This suggested that roots hydraulics were a substantial limitation to water flow in pearl millet, especially under high VPD. The dependence on the apoplastic and symplastic water transport pathways was investigated by assessing the transpiration response of plants treated with inhibitors specific to the AQP-mediated symplastic pathway (AgNO3 and H2O2) and to the apoplastic pathway (precipitates of Cu(Fe(CN)6) or Cu(CuFe(CN)6)). When CuSO4 alone was used, Cu ions caused an inhibition of transpiration in both genotypes and more so in H77/833-2. The transpiration of high Tr H77/833-2 was decreased more by AQP inhibitors under low VPD (1.8 kPa) than in PRLT 2/89/33, whereas under high VPD (4.2 kPa), the transpiration of PRLT 2/89/33 was decreased more by AQP inhibitors than in H77/833-2. The transpiration rate of detached leaves from H77/833-2 when treated with AgNO3 decreased more than in PRLT 2/89/33. Although the root hydraulic conductivity of both genotypes was similar, it decreased more upon the application of a symplastic inhibitor in H77/833-2. The transpiration of low Tr PRLT 2/89/33 was decreased more by apoplastic inhibitors under both low and high VPD. Then the hydraulic conductivity decreased more upon the application of an apoplastic inhibitor in PRLT 2/89/33. In conclusion, both pathways contributed to water transport, and their contribution varied with environmental conditions and genotypes. Roots were a main source of hydraulic limitation in these genotypes of pearl millet, although a leaf limitation was not excluded. The similarity between genotypes in root hydraulic conductivity under normal conditions also suggests changes in this conductivity upon changes in the evaporative demand. The low Tr genotype depended more on the apoplastic pathway for water transport, whereas the high Tr genotype depended on both pathway, may be by ‘tuning-up’ the symplastic pathway under high transpiration demand, very likely via the involvement of aquaporins.

scholarly journals Constraints to Pearl Millet (Pennisetum glaucum) Production and Farmers’ Approaches to Striga hermonthica Management in Burkina Faso

2021 ◽  
Vol 13 (15) ◽  
pp. 8460
Author(s):  
Armel Rouamba ◽  
Hussein Shimelis ◽  
Inoussa Drabo ◽  
Mark Laing ◽  
Prakash Gangashetty ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Pearl Millet ◽  
Management Practices ◽  
Pennisetum Glaucum ◽  
Control Measures ◽  
Striga Hermonthica ◽  
Soil Conditions ◽  
Management Options ◽  
Central Plateau ◽  
South Central

Pearl millet (Pennisetum glaucum) is a staple food crop in Burkina Faso that is widely grown in the Sahelian and Sudano-Sahelian zones, characterised by poor soil conditions and erratic rainfall, and high temperatures. The objective of this study was to document farmers’ perceptions of the prevailing constraints affecting pearl millet production and related approaches to manage the parasitic weeds S. hermonthica. The study was conducted in the Sahel, Sudano-Sahelian zones in the North, North Central, West Central, Central Plateau, and South Central of Burkina Faso. Data were collected through a structured questionnaire and focus group discussions involving 492 participant farmers. Recurrent drought, S. hermonthica infestation, shortage of labour, lack of fertilisers, lack of cash, and the use of low-yielding varieties were the main challenges hindering pearl millet production in the study areas. The majority of the respondents (40%) ranked S. hermonthica infestation as the primary constraint affecting pearl millet production. Respondent farmers reported yield losses of up to 80% due to S. hermonthica infestation. 61.4% of the respondents in the study areas had achieved a mean pearl millet yields of <1 t/ha. Poor access and the high cost of introduced seed, and a lack of farmers preferred traits in the existing introduced pearl millet varieties were the main reasons for their low adoption, as reported by 32% of respondents. S. hermonthica management options in pearl millet production fields included moisture conservation using terraces, manual hoeing, hand weeding, use of microplots locally referred to as ‘zaï’, crop rotation and mulching. These management techniques were ineffective because they do not suppress the below ground S. hermonthica seed, and they are difficult to implement. Integrated management practices employing breeding for S. hermonthica resistant varieties with the aforementioned control measures could offer a sustainable solution for S. hermonthica management and improved pearl millet productivity in Burkina Faso.

scholarly journals The Singular Molecular Conformation of Humic Acids in Solution Influences Their Ability to Enhance Root Hydraulic Conductivity and Plant Growth

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 3
Author(s):  
Maite Olaetxea ◽  
Veronica Mora ◽  
Roberto Baigorri ◽  
Angel M. Zamarreño ◽  
Jose M. García-Mina
Keyword(s):  
Polyethylene Glycol ◽  
Humic Acid ◽  
Hydraulic Conductivity ◽  
Plant Growth ◽  
Polyacrylic Acid ◽  
Molecular Conformation ◽  
Water Solution ◽  
Biological Effects ◽  
Root Hydraulic Conductivity ◽  
Molecular Systems

Some studies have reported that the capacity of humic substances to improve plant growth is dependent on their ability to increase root hydraulic conductivity. It was proposed that this effect is directly related to the structural conformation in solution of these substances. To study this hypothesis, the effects on root hydraulic conductivity and growth of cucumber plants of a sedimentary humic acid and two polymers—polyacrylic acid and polyethylene glycol—presenting a molecular conformation in water solution different from that of the humic acid have been studied. The results show that whereas the humic acid caused an increase in root hydraulic conductivity and plant growth, both the polyacrylic acid and the polyethylene glycol did not modify plant growth and caused a decrease in root hydraulic conductivity. These results can be explained by the different molecular conformation in water solution of the three molecular systems. The relationships between these biological effects and the molecular conformation of the three molecular systems in water solution are discussed.

scholarly journals Effects of Cover Crops, Compost, and Vermicompost on Strawberry Yields and Nitrogen Availability in North Carolina

2016 ◽  
Vol 26 (5) ◽  
pp. 604-613 ◽  
Author(s):  
John E. Beck ◽  
Michelle S. Schroeder-Moreno ◽  
Gina E. Fernandez ◽  
Julie M. Grossman ◽  
Nancy G. Creamer
Keyword(s):  
North Carolina ◽  
Pearl Millet ◽  
Cover Crops ◽  
Cover Crop ◽  
Crop Yields ◽  
Pennisetum Glaucum ◽  
Organic Production ◽  
Organic N ◽  
N Availability ◽  
Soil N

Summer cover crop rotations, compost, and vermicompost additions can be important strategies for transition to organic production that can provide various benefits to crop yields, nitrogen (N) availability, and overall soil health, yet are underused in strawberry (Fragaria ×ananassa) production in North Carolina. This study was aimed at evaluating six summer cover crop treatments including pearl millet (Pennisetum glaucum), soybean (Glycine max), cowpea (Vigna unguiculata), pearl millet/soybean combination, pearl millet/cowpea combination, and a no cover crop control, with and without vermicompost additions for their effects on strawberry growth, yields, nutrient uptake, weeds, and soil inorganic nitrate-nitrogen and ammonium-nitrogen in a 2-year field experiment. Compost was additionally applied before seeding cover crops and preplant N fertilizer was reduced by 67% to account for organic N additions. Although all cover crops (with compost) increased soil N levels during strawberry growth compared with the no cover crop treatment, cover crops did not impact strawberry yields in the first year of the study. In the 2nd year, pearl millet cover crop treatments reduced total and marketable strawberry yields, and soybean treatments reduced marketable strawberry yields when compared with the no cover crop treatment, whereas vermicompost additions increased strawberry biomass and yields. Results from this study suggest that vermicompost additions can be important sustainable soil management strategies for transitional and certified organic strawberry production. Summer cover crops integrated with composts can provide considerable soil N, reducing fertilizer needs, but have variable responses on strawberry depending on the specific cover crop species or combination. Moreover, these practices are suitable for both organic and conventional strawberry growers and will benefit from longer-term studies that assess these practices individually and in combination and other benefits in addition to yields.

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