scholarly journals Pengaruh Nitrat terhadap Biokorosi Logam oleh Konsorsium Bakteri Pereduksi Sulfat dari PLTA Saguling

2021 ◽  
Vol 6 (2) ◽  
pp. 71
Author(s):  
Nur Fitriah Afianti ◽  
Dea Indriani Astuti
Keyword(s):  
Corrosion Rate ◽  
Cooling System ◽  
Sulfate Reducing Bacteria ◽  
Maintenance Cost ◽  
Sulfate Reducing ◽  
Dsrb Gene ◽  
Final Electron ◽  
Reducing Bacteria ◽  
Equipment Lifetime ◽  
Final Electron Acceptor

<div><strong>The Influence of Nitrate in Metal Biocorrosion caused by Sulfate Reducing Bacteria from Saguling Hydropower</strong>. The corrosion facilitated and accelerated by the activities of microorganism is called biocorrosion. Sulfate reducing bacteria (SRB) is known as the bacteria that cause biocorrosion in anaerobic condition by using sulfate as the final electron acceptor. Biocorrosion reduces equipment lifetime and increases maintenance cost in industry. In the cooling system in Saguling hydropower, corrosion was commonly caused by utilization of contaminated water due to anorganic and organic waste, especially sulfate. In this research, sulfate reducing bacteria was isolated from biofilms in the cooling system of Saguling Hydropower. Molecular analysis using PCR-DGGE method with dsrB gene (350 bp) as molecular markers showed that SRB consortium contained 12 bands and assumed as different species of SRB. SRB consortium was tested to determine its biocorrosion activity over metal material of ST37 (carbon steel) and SUS304 (stainless steel). The consortium then treated with 7 different nitrate concentrations to determine its effect against the sulfate reducing bacteria activity. SRB consortium caused higher corrosion to ST37 than SUS304L, with the corrosion rate of 0.07660 mm/year and 0.00265 mm/year, respectively. Concentration of 10 mM nitrate effectively inhibited corrosion rate on ST37 and caused the changes in sulfate reducing bacteria communities, indicated by the disappearance of 6 bands in DGGE profile</div>

scholarly journals Microbial Corrosion of API 5L X-70 Carbon Steel by ATCC 7757 and Consortium of Sulfate-Reducing Bacteria

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Arman Abdullah ◽  
Nordin Yahaya ◽  
Norhazilan Md Noor ◽  
Rosilawati Mohd Rasol
Keyword(s):  
Weight Loss ◽  
Carbon Steel ◽  
Corrosion Rate ◽  
Sulfate Reducing Bacteria ◽  
Desulfovibrio Vulgaris ◽  
Corrosion Morphology ◽  
Sulfate Reducing ◽  
Weight Loss Method ◽  
Loss Method ◽  
Reducing Bacteria

Various cases of accidents involving microbiology influenced corrosion (MIC) were reported by the oil and gas industry. Sulfate reducing bacteria (SRB) have always been linked to MIC mechanisms as one of the major causes of localized corrosion problems. In this study, SRB colonies were isolated from the soil in suspected areas near the natural gas transmission pipeline in Malaysia. The effects of ATCC 7757 and consortium of isolated SRB upon corrosion on API 5L X-70 carbon steel coupon were investigated using a weight loss method, an open circuit potential method (OCP), and a potentiodynamic polarization curves method in anaerobic conditions. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were then used to determine the corrosion morphology in verifying the SRB activity and corrosion products formation. Results from the study show that the corrosion rate (CR) of weight loss method for the isolated SRB is recorded as 0.2017 mm/yr compared to 0.2530 mm/yr for ATCC 7757. The Tafel plot recorded the corrosion rate of 0.3290 mm/yr for Sg. Ular SRB and 0.2500 mm/yr forDesulfovibrio vulgaris. The results showed that the consortia of isolated SRB were of comparable effects and features with the single ATCC 7757 strain.

DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria

2006 ◽  
Vol 66 (2) ◽  
pp. 194-205 ◽  
Author(s):  
Joke Geets ◽  
Brigitte Borremans ◽  
Ludo Diels ◽  
Dirk Springael ◽  
Jaco Vangronsveld ◽  
...  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Dsrb Gene ◽  
Reducing Bacteria

Influences of Thermophilic Sulfate-Reducing Bacteria on the Corrosion of Carbon Steel at Different Temperature

2011 ◽  
Vol 396-398 ◽  
pp. 1963-1968
Author(s):  
Shi Hong Zhang ◽  
Fei Kuang ◽  
Jin Zhong Zhang ◽  
Xiong Zhou ◽  
Gui Hong Lan ◽  
...  
Keyword(s):  
Carbon Steel ◽  
Corrosion Rate ◽  
Growth Curves ◽  
Sulfate Reducing Bacteria ◽  
Reduction Product ◽  
Stable Phase ◽  
Sulfate Reducing ◽  
Corrosion Behaviors ◽  
Higher Temperature ◽  
Reducing Bacteria

The growth and the corrosion behaviors of thermophilic sulfate reducing bacteria (SRB), desulfotomaulum thermocisternum strain ST90, were studied in this work. It was found that the growth curves and the main reduction products were influenced greatly by the growth temperatures. The time of the exponential phase and the stable phase decreased with the increase of growth temperatures. The reduction product of this kind of SRB was mainly H2S when it grew at 60°C. With the decrease of growth temperatures, thiosulfate came up as an internal reduction product. The thiosulfate was re-reduced by SRB at the growth temperatures of 40 and 50°C. When the growth temperature came to 30°C, no H2S was generated. The regulation of corrosion rate changed was coincident with the changes of the main reduction products of the SRB. This kind of SRB could accelerate the corrosion rate of carbon steel when grew at higher temperature. But the corrosion rate of carbon steel could be inhibited when it grew at 30°C.

scholarly journals Desulfovibrio sp. Genes Involved in the Respiration of Sulfate during Metabolism of Hydrogen and Lactate

2002 ◽  
Vol 68 (4) ◽  
pp. 1932-1937 ◽  
Author(s):  
Jennifer L. Steger ◽  
Carr Vincent ◽  
Jimmy D. Ballard ◽  
Lee R. Krumholz
Keyword(s):  
Transcriptional Control ◽  
Cell Mass ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Northern Blotting ◽  
Sulfate Reducing ◽  
Transcriptional Changes ◽  
Final Electron ◽  
Arbitrarily Primed Pcr ◽  
Reducing Bacteria

ABSTRACT To develop a better understanding of respiration by sulfate-reducing bacteria, we examined transcriptional control of respiratory genes during growth with lactate or hydrogen as an electron donor. RNA extracts of Desulfovibrio desulfuricans subsp. aestuarii were analyzed by using random arbitrarily primed PCR. RNA was reverse transcribed under low-stringency conditions with a set of random primers, and candidate cDNAs were cloned, sequenced, and characterized by BLAST analysis. Putative differentially expressed transcripts were confirmed by Northern blot analysis. Interestingly, dissimilatory bisulfite reductase was upregulated in the presence of hydrogen. To link these transcriptional changes to the physiology of sulfate-reducing bacteria, sulfide was measured during growth of several strains of Desulfovibrio on hydrogen or lactate, and this revealed that hydrogen-grown cells produced more sulfide per unit of cell mass than lactate-grown cells. Transcription of other redox proteins was characterized by Northern blotting to determine whether or not they were also transcribed to higher levels in hydrogen-grown cells. Growth on lactate produced greater transcription of [NiFe] hydrogenase. H2-grown cells transcribed the adenylylsulfate reductase b subunit and HmcA to higher levels. The results we describe here provide new insight into the continuing debate over how Desulfovibrio species utilize redox components to generate membrane potential and to channel electrons to sulfate, the final electron acceptor.

Specificity of Lectins Labeled with Colloidal Gold to the Exopolymeric Matrix Carbohydrates of the Sulfate-Reducing Bacteria Biofilm Formed on Steel

2020 ◽  
Vol 82 (5) ◽  
pp. 11-20
Author(s):  
D.R. Abdulina ◽  
◽  
L.M. Purish ◽  
G.O. Iutynska ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Colloidal Gold ◽  
Steel Surface ◽  
Lectin Binding ◽  
Sulfate Reducing Bacteria ◽  
Gold Particles ◽  
Sulfate Reducing ◽  
Transmission Electron ◽  
Reducing Bacteria

The studies of the carbohydrate composition of the sulfate-reducing bacteria (SRB) biofilms formed on the steel surface, which are a factor of microbial corrosion, are significant. Since exopolymers synthesized by bacteria could activate corrosive processes. The aim of the study was to investigate the specificity of commercial lectins, labeled with colloidal gold to carbohydrates in the biofilm exopolymeric matrix produced by the corrosive-relevant SRB strains from man-caused ecotopes. Methods. Microbiological methods (obtaining of the SRB biofilms during cultivation in liquid Postgate B media under microaerophilic conditions), biochemical methods (lectin-binding analysis of 10 commercial lectins, labeled with colloidal gold), transmission electron microscopy using JEM-1400 JEOL. Results. It was shown using transmission electron microscopy that the binding of lectins with carbohydrates in the biofilm of the studied SRB strains occurred directly in the exopolymerіс matrix, as well as on the surfaces of bacterial cells, as seen by the presence of colloidal gold particles. For detection of the neutral carbohydrates (D-glucose and D-mannose) in the biofilm of almost all studied bacterial strains PSA lectin was the most specific. This lectin binding in biofilms of Desulfotomaculum sp. К1/3 and Desulfovibrio sp. 10 strains was higher in 90.8% and 94.4%, respectively, then for ConA lectin. The presence of fucose in the SRB biofilms was detected using LABA lectin, that showed specificity to the biofilm EPS of all the studied strains. LBA lectin was the most specific to N-аcetyl-D-galactosamine for determination of amino sugars in the biofilm. The amount of this lectin binding in D. vulgaris DSM644 biofilm was 30.3, 10.1 and 9.3 times higher than SBA, SNA and PNA lectins, respectively. STA, LVA and WGA lectins were used to detect the N-acetyl-Dglucosamine and sialic acid in the biofilm. WGA lectin showed specificity to N-acetyl-D-glucosamine in the biofilm of all the studied SRB; maximum number of bounded colloidal gold particles (175 particles/μm2) was found in the Desulfotomaculum sp. TC3 biofilm. STA lectin was interacted most actively with N-acetyl-D-glucosamine in Desulfotomaculum sp. TC3 and Desulfomicrobium sp. TC4 biofilms. The number of bounded colloidal gold particles was in 9.2 and 7.4 times higher, respectively, than using LVA lectin. The lowest binding of colloidal gold particles was observed for LVA lectin. Conclusions. It was identified the individual specificity of the 10 commercial lectins to the carbohydrates of biofilm matrix on the steel surface, produced by SRB. It was estimated that lectins with identical carbohydrates specificity had variation in binding to the biofilm carbohydrates of different SRB strains. Establishing of the lectin range selected for each culture lead to the reduction of the scope of studies and labor time in the researching of the peculiarities of exopolymeric matrix composition of biofilms formed by corrosiverelevant SRB.

The influence of potassium dichromate on dissimilatory reduction of sulfate and nitrate ions by bacteria Desulfovibrio sp.

2017 ◽  
Vol 28 (1-2) ◽  
pp. 84-95
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
Ch. I. Bohoslavets ◽  
T. M. Hrytsun’ ◽  
B. M. Borsukevych
Keyword(s):  
Electron Acceptor ◽  
Potassium Dichromate ◽  
Anaerobic Respiration ◽  
Sulfate Reducing Bacteria ◽  
Negative Influence ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Nitrate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reducing bacteria, capable to reductive transformation of different nature pollutants, used in biotechnologies of purification of sewage, contaminated by carbon, sulfur, nitrogen and metal compounds. H2S formed by them sediment metals to form of insoluble sulfides. Number of metals can be used by these microorganisms as electron acceptors during anaerobic respiration. Because under the influence of metal compounds observed slowing of bacteria metabolism, selection isolated from technologically modified ecotops resistant to pollutions strains is important task to create a new biotechnologies of purification. That’s why the purpose of this work was to study the influence of potassium dichromate, present in medium, on reduction of sulfate and nitrate ions by sulfate reducing bacteria Desulfovibrio desulfuricans IMV K-6, Desulfovibrio sp. Yav-6 and Desulfovibrio sp. Yav-8, isolated from Yavorivske Lake, to estimate the efficiency of possible usage of these bacteria in technologies of complex purification of environment from dangerous pollutants. Bacteria were cultivated in modified Kravtsov-Sorokin medium without SO42- and FeCl2×4H2O for 10 days. To study the influence of K2Cr2O7 on usage by bacteria SO42- or NO3- cells were seeded to media with Na2SO4×10H2O or NaNO3 and K2Cr2O7 at concentrations of 1.74 mM for total content of electron acceptors in medium 3.47 mM (concentration of SO42- in medium of standard composition). Cells were also seeded to media with 3.47 mM Na2SO4×10H2O, NaNO3 or K2Cr2O7 to investigate their growth in media with SO42-, NO3- or Cr2O72- as sole electron acceptor (control). Biomass was determined by turbidymetric method, content of sulfate, nitrate, dichromate, chromium (III) ions, hydrogen sulfide or ammonia ions in cultural liquid – by spectrophotometric method. It was found that K2Cr2O7 inhibits growth (2.2 and 1.3 times) and level of reduction by bacteria sulfate or nitrate ions (4.2 and 3.0 times, respectively) at simultaneous addition into cultivation medium of 1.74 mM SO42- or NO3- and 1.74 mM Cr2O72-, compared with growth and level of reduction of sulfate or nitrate ions in medium only with SO42- or NO3- as sole electron acceptor. Revealed that during cultivation of bacteria in presence of equimolar amount of SO42- or NO3- and Cr2O72-, last used by bacteria faster, content of Cr3+ during whole period of bacteria cultivation exceeded content H2S or NH4+. K2Cr2O7 in medium has most negative influence on dissimilatory reduction by bacteria SO42- than NO3-, since level of nitrate ions reduction by cells in medium with NO3- and Cr2O72- was a half times higher than level of sulfate ions reduction by it in medium with SO42- and Cr2O72-. The ability of bacteria Desulfovibrio sp. to priority reduction of Cr2O72- and after their exhaustion − NO3- and SO42- in the processes of anaerobic respiration can be used in technologies of complex purification of environment from toxic compounds.

Removal of lead by sulfate-reducing bacteria in anaerobic continuous stirred tank reactors

2016 ◽  
Vol 14 (3) ◽  
pp. 557-561
Author(s):  
Nguyễn Thị Yên ◽  
Kiều Thị Quỳnh Hoa
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Stirred Tank ◽  
Synthetic Wastewater ◽  
Terminal Electron Acceptor ◽  
Stirred Tank Reactors ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Continuous Stirred Tank ◽  
Continuous Stirred Tank Reactors ◽  
Reducing Bacteria

Lead contaminated wastewater negatively impacts to living organisms as well as humans. In recent years, a highly promising biological process using the anaerobic production of sulfide ions by sulfate-reducing bacteria has presented itself as an alternative option for the removal of lead. This process is based on microbial utilization of electron donors, such as organic compounds (carbon sources), and sulfate as the terminal electron acceptor for sulfide production. The biogenic hydrogen sulfide reacts with dissolved heavy metals to form insoluble metal sulfide precipitates Removal of lead by an enriched consortium of sulfate-reducing bacteria (DM10) was evaluated sulfate reduction, sulfide production and lead precipitation. Four parallel anaerobic continuous stirred tank reactors (CSTR, V = 2L) (referred as R1 - R4) were fed with synthetic wastewater containing Pb2+ in the concentrations of 0, 100, 150 and 200 mg L-1 of lead and operated with a hydraulic retention time of 5 days for 40 days. The loading rates of each metal in R1- R4 were 0, 20, 30 and 40 mg L-1 d-1, respectively. The results showed that there was no inhibition of SRB growth and that lead removal efficiencies of 99-100% for Pb2+ were achieved in R2 (100 mg L-1) and R3 (150 mg L-1) throughout the experiment. For the highest lead concentration of  200 mg L-1, a decrease in efficiency of removal (from 100 to 96%) was observed at the end of the experiment. The obtained result of this study might help for a better control operation and performance improvements of reactors.

Sign in / Sign up

Export Citation Format

Share Document