scholarly journals Cytotoxic Activity of Ethyl Acetate Fraction of Aglaia elliptica Blume Leaves Extract on HepG2 Hepatocarcinoma Cells

2018 ◽  
Vol 9 (3) ◽  
pp. 143
Author(s):  
Churiyah Churiyah ◽  
Ekclesia Yuliarni ◽  
Agung Eru Wibowo ◽  
Firdayani Firdayani
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Biological Activities ◽  
Enzymatic Reaction ◽  
Docking Simulation ◽  
Ethyl Acetate Fraction ◽  
Cytotoxic Activities ◽  
Inhibition Of Proliferation ◽  
Diphenyltetrazolium Bromide ◽  
Hepatocarcinoma Cells

Aglaia elliptica Blume belongs to Meliaceae family which contain an active compound of rocaglamid as anticancer. The research was conducted to evaluate the biological activities of ethyl acetate fraction of Aglaia elliptica Blume leaves. The dry powder of Aglaia elliptica Blume leaves was extracted with methanol using maceration method, then was fractionated using n-hexane and ethyl acetate, guided by brine shrimp lethality test (BSLT). The most toxic fraction then processed by column chromatography and resulted in eight subfractions. Three of them showed the most toxic effect on BSLT namely FEA 3.3, FEA 3.4 and FEA 3.5 with LC50 of 40.81, 18.56 and 13.40 ppm respectively. The cytotoxic activities of those three active subfractions were assayed on HepG2 hepatocarcinoma cells using enzymatic reaction of 3-(4,5-dimethylthiazoyl-2-yl) 2,5 diphenyltetrazolium bromide (MTT), and the results showed that the IC50 were 35.10, 14.36 and 14.09 ppm respectively. The most active sub fraction (FEA 3.5) was then performed further analyzed using preparative high performance liquid chromatography (HPLC). The HPLC results indicated that there were three active compounds, which were suspected as derivatives of rocaglamid. The molecular docking simulation indicated that rocaglamide formed complex with Toll-like Receptor 4 in HepG2 hepatocarcinoma cells and affected the inhibition of proliferation of its cell. Keywords : Aglaia elliptica Blume leaves extract, fractionation, BSLT, MTT assay,hepatocarsinoma cell lines

scholarly journals Antioxidant, Antibacterial, Cytotoxic, and Anti-Inflammatory Potential of the Leaves ofSolanum lycocarpumA. St. Hil. (Solanaceae)

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Guilherme Augusto Ferreira da Costa ◽  
Melissa Grazielle Morais ◽  
Aline Aparecida Saldanha ◽  
Izabela Caputo Assis Silva ◽  
Álan Alex Aleixo ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Cytotoxic Activities ◽  
Phenolic Composition ◽  
Cytotoxic Potential ◽  
Phenolic Constituents ◽  
Anti Inflammatory ◽  
Later Phase

Ethanol extract and fractions obtained from leaves ofSolanum lycocarpumwere examined in order to determine their phenolic composition, antioxidant, antibacterial, anti-inflammatory, and cytotoxic potential. High performance liquid chromatography coupled with DAD analysis indicated that the flavonoids apigenin and kaempferol were the main phenolic compounds present in dichloromethane and ethyl acetate fractions, respectively. The antioxidant activity was significantly more pronounced for dichloromethane, ethyl acetate, and hydroethanol fractions than that of the commercial antioxidant 2,6-di-tert-butyl-4-methylphenol. The hexane and dichloromethane fractions were more active against the tested bacteria. The hydroethanol fraction exhibited significant anti-inflammatory activity at the dose of 75 and 150 mg/kg in the later phase of inflammation. However, the antiedematogenic effect of the higher dose of the ethyl acetate fraction (150 mg/kg) was more pronounced. The ethyl acetate fraction also presented a less cytotoxic effect than the ethanol extract and other fractions. These activities found inS. lycocarpumleaves can be attributed, at least in part, to the presence of phenolic constituents such as flavonoids. This work provided the knowledge of phenolic composition in the extract and fractions and the antioxidant, antibacterial, anti-inflammatory, and cytotoxic activities of leaves ofS. lycocarpum.

scholarly journals Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

2014 ◽  
Vol 86 (2) ◽  
pp. 881-888 ◽  
Author(s):  
MARIANA PIANA ◽  
ALINE A. BOLIGON ◽  
THIELE F. DE BRUM ◽  
MARINA ZADRA ◽  
BIANCA V. BELKE ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Condensed Tannins ◽  
High Performance ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

Exploring cytotoxic and antioxidant properties of Heliotropium calcareum in polar and non-polar extracts

2021 ◽  
Vol 02 ◽  
Author(s):  
Mohammad Uzair ◽  
Faisal Rashid ◽  
Hamid Saeed Shah ◽  
Jamshed Iqbal
Keyword(s):  
Cell Cycle ◽  
Ethyl Acetate ◽  
Hela Cells ◽  
Biological Activities ◽  
Research Work ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Cell Cycle Analysis ◽  
G1 Phase ◽  
Traditional Use

Background: Plants are a vital source of natural drugs as the traditional use of plants as therapeutic agents for a variety of ailments has been traced back to thousands of years. The utilization of Heliotropium calcareum has been evident since ancient times for treating various disease states like inflammation associated with gout and rheumatism, poisonous bites, and other skin disorders. The current research work was carried out to determine the phytochemistry and biological activities of the crude methanolic extract obtained through maceration from the aerial parts of Heliotropium calcareum. Methods: The plant was collected from district Bhakkar, Punjab, Pakistan. Maximum phenolic (74.5 µg GAE/mg) and flavonoid content (58.99 µg QE/mg) were observed in ethyl acetate fraction. Significant antioxidant potential was observed in ethyl acetate fraction with the highest free radical hunting activity of 92.6 ± 6.7 µM. Results: Cytotoxicity assay using MTT dye was performed where non-polar (n-hexane) and polar (ethyl acetate) fractions displayed excellent cytotoxicity against HeLa cells (IC50 = 79.95 ± 3.718 & 164 ± 4 µg/mL respectively). Furthermore, the above fractions showed momentous results in cell cycle analysis and promising proapoptotic effect against cervical (HeLa) cancer cell lines. An n-hexane and ethyl acetate fraction were selected for cell cycle analysis to determine the quantitative measurement of the degree of apoptosis. According to the results given below in the figure, the cervical (HeLa) cancer cells were treated with n-hexane and ethyl acetate fractions at various concentrations. An increase in the cell population at G0/G1 phase and a decrease in the S-phase population concerning untreated cells suggested the G0/G1 phase arrest in n-hexane and ethyl acetate fractions treated HeLa cells. Conclusion: Overall, , n-hexane and ethyl acetate fractions were found to be the most promising and active elements of H. calcareum and may be utilized to explore their cytotoxic effects further in the animal model.

In Vitro Screening for Cytotoxic, Anti-bacterial, Anti-HIV1-RT Activities and Chemical Constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii

2021 ◽  
Vol 11 ◽  
Author(s):  
Charina Worarat ◽  
Wilart Pompimon ◽  
Phansuang Udomputtimekakul ◽  
Sukee Sukdee ◽  
Punchavee Sombutsiri ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Bacterial Activity ◽  
Cytotoxic Activities ◽  
Separation And Purification ◽  
Crude Extracts ◽  
Cytotoxicity Activities

Background: Although the chemical constituents and biological activities of a large number of plants in the Croton genus have been studied, there are still recently discovered plants to be investigated. Objective: 1. To investigate the anti-bacterial, anti-HIV1-RT, and cytotoxicity activities of crude extracts from these plants. 2. To investigate the chemical constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii. Method: The anti-bacterial, anti-HIV1-RT, and cytotoxicity of the three plants were evaluated by standard techniques. Extraction, separation, and purification of extracts from the three plants were undertaken. Results: The ethyl acetate extract of C. fluviatilis showed low anti-bacterial activity against E. aerogenes, E. coli 0157: H7, and P. mirabilis, together with the ethyl acetate extract of C. acutifolius displayed low anti-bacterial activity against E. aerogenes, while all the crude extracts of C. thorelii were inactive. The ethyl acetate extracts of C. thorelii, and C. fluviatilis showed strong inhibited HIV1-RT, whereas the ethyl acetate extract of C. acutifolius, and the hexane extract of C. fluviatilis displayed moderate inhibited HIV1-RT. Cytotoxic properties of three Croton plants were specific to KKU-M213, MDA-MB-231, A-549, and MMNK-1. Especially, the ethyl acetate extract of C. acutifolius exhibited strong cytotoxic activities against MDA-MB-231, A-549, and MMNK-1. Furthermore, the ethyl acetate extract of C. thorelii showed high cytotoxic activities against KKU-M213, and MDA-MB-231. Compounds 1, and 4 were found in C. fluviatilis. Compounds 2 and 4 were also found in C. acutifolius. Moreover, compound 3 was only found in C. thorelii. Conclusion: The present study revealed that the three Croton species are good sources of flavonoid compounds and further investigation of the chemical constituents from these plants may prove to be fruitful to discover more active compounds to be tested as potential medicines.

scholarly journals Study on biological activities of extracted fractions from Typhonium flagelliforme (Lodd.) Blume

2017 ◽  
Vol 33 (2S) ◽  
Author(s):  
Le Quy Thuong ◽  
Bach Tuyet Mai ◽  
Nguyen Minh Chau ◽  
Le Thi Phuong Hoa ◽  
Nguyen Quang Huy
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Biological Activities ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Chemical Compositions ◽  
Active Components ◽  
Stomach Pain ◽  
Ic50 Values

Typhonium flagelliforme is a medicinal plant that has variety of uses. In medicinal traditional T. flagelliforme is used to treatment cough, headache, stomach pain chronic, and tracheitis. Moreover, use fresh bulbs treatment furuncle, the bites of poisonous insects. The active components in T. flagelliforme are flavonoids. In this study, the T. flagelliforme extract was obtained by methanol  to determine the chemical composition. Then, The extracts of methanol are extracted with polarization increases gradually solvents such as haxane, dichloromethane and ethyl acetate. Determination of antioxidant activity, cytotoxic activity of extracted fractions. Results obtained showed that the chemical compositions by the qualitative reaction preliminary were identified from T. flagelliforme containing reducing sugars, amino acids, organic acids, flavonoids, alcaloids, sterols. The antioxidant capacity of the ethyl acetate fraction reached 94.76 μg/ml, 10 times higher than the positive control is Quercetin. Cytotoxic activity of the haxane and diclomethane extracted fractions from T. flagelliforme exhibited cytotoxic activity on all three experimental cancers cell lines: KB, HepG2, Lu after 72h of culture with IC50 values ​​range from 92.8 to 107.76 μg/ml. From dichlomethane extracted of T. flagelliforme was purified TF1 as Stigmast-4-en- 3-on.    

scholarly journals DNA Barcode Authentication and Improvement of Andrographolide Yield in Andrographis paniculata Plant

2021 ◽  
Vol 16 (2) ◽  
pp. 117-125
Author(s):  
B. A. Oseni ◽  
C. P. Azubuike ◽  
O. O. Okubanjo
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Dna Barcode ◽  
Bioactive Compound ◽  
Dry Weight ◽  
Ethyl Acetate Fraction ◽  
Andrographis Paniculata ◽  
Dna Barcodes ◽  
Medicinal Value ◽  
And Control

Background: Andrographolide, the major bioactive compound responsible for most pharmacological activities such as anticancer, antimicrobial activity exhibited by the Andrographis paniculata plant is present in small quantities. In addition, the genus Andrographis has about 28 species most of which possess no medicinal value. The deoxyribonucleic acid (DNA) barcode is utilized in species identification and plant authentication.Objectives: This study aimed at authenticating Andrographis paniculata using DNA barcodes and improving the yield of andrographolide via enzymatic treatment.Materials and Method: The DNA of Andrographis plant was obtained using the Qiagen kit. The psbA-trnH and rbcL DNA barcode regions were amplified using polymerase chain reaction (PCR). Presence of amplified regions was confirmed using gel electrophoresis and the amplicons were sequenced. A blast N search was performed on the sequenced DNA. The constituents of A. paniculata dried leaves was extracted using methanol, followed by treatment with and without β glucosidase. The extract obtained was dried and partitioned using ethyl acetate. The ethyl acetate fraction was concentrated and dissolved in methanol. Andrographolide content was determined using high performance liquid chromatography (HPLC).Results: The psbA-trnH and rbcL DNA regions were successfully amplified having 358 and 604 bp respectively. The DNA barcode sequences obtained were identical to the psbA-trnH (97%) and rbcL (99%) genes of A. paniculata voucher MICET P00101. The mean andrographolide yield was 9.4±0.11mg/g and 8.9±0.13mg/g dry weight for the treatment and control groups respectively; statistical analysis at p = 0.05 shows a significant difference.Conclusion: The Andrographis plant used in this study was confirmed to be Andrographis paniculata, enzymatic treatment increased andrographolide yield from the plant. Keywords: Andrographis paniculata, andrographolide, authentication, DNA barcodes, β-glucosidase.

ETHYL ACETATE EXTRACT OF Chenopodium murale ROOT, A SOURCE OF BIOACTIVE COMPOUNDS

2021 ◽  
Vol 27 (1) ◽  
pp. 93-100
Author(s):  
Arshad Javaid ◽  
Syeda Fakehha Naqvi ◽  
Iqra Haider Khan
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Methyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Ethyl Acetate Fraction ◽  
Dioctyl Phthalate ◽  
Literature Survey ◽  
Root Extract ◽  
Chenopodium Murale

Chenopodium murale L. is a winter weed of Chenopodiaceae. In this study, bioactive compounds present in ethyl acetate fraction of root extract of C. murale were identified. The weed plants were collected from Jehlem, Pakistan. Its roots were dried, powdered and extracted in methanol. After evaporation of the solvent, the remaining extract was mixed in water and partitioned with n-hexane, chloroform and finally with ethyl acetate. The last fraction was analyzed through GC-MS that indicated the presence of 15 compounds. These included the three major compounds namely o-xylene (15.03%), cyclopentanol (13.42) and 2-hexanol (13.99%). The moderately and less abundant compounds were ethylbenzene (5.47); methyl acetate (6.00%); cholestrol (4.33%); 2-phenanthrenol (3.01%); cyclohexanone (5.32%); p-xylene (5.12%); furostan-3,26-diyl dibenzoate (3.29%); dihexyl phthalate (4.99%); tricosanoic acid (2.74%); dioctyl phthalate (4.99%), hexanal (3.05%) and ergostane (1.29%). Literature survey showed that 10 of the identified compounds exhibited various biological activities including antifungal, antibacterial, antioxidant, anticancer and antipsoriatic. Most of the compounds were antimicrobial in nature.

Development and Validation of a High-Performance Liquid Chromatography Method for Standardization of the Bioactive Ethyl Acetate Fraction of Alstonia scholaris (Linn.) R. Br. Growing in Egypt

2013 ◽  
Vol 68 (9-10) ◽  
pp. 376-383
Author(s):  
Hesham I. El-Askary ◽  
Mahmoud M. El-Olemy ◽  
Maha M. Salama ◽  
Mahetab H. Amer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
High Performance ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Hplc Method ◽  
Spectroscopic Techniques ◽  
Alstonia Scholaris ◽  
Relative Standard

Bio-guided fractionation of the ethanolic extract of the leaves of Alstonia scholaris (Apocynaceae) growing in Egypt was carried out to evaluate its antihyperglycemic acti vity in alloxan-induced diabetic rats and its hepatoprotective activity against CCl4-induced hepatotoxicity in rats. The ethyl acetate fraction of the ethanolic extract showed the highest antihyperglycemic [(133.6 ± 4.2) mg/mL, relative to metformin with (92.3 ± 2.7) mg/mL] and hepatoprotective [(37.9 ± 1.4) U/L, relative to silymarin with (29.7 ± 0.8) U/L] activities. Four compounds were isolated from this fraction, and identifi ed by spectroscopic techniques and by comparison with reported data: caffeic acid and isoquercitrin for the fi rst time from this plant, in addition to quercetin 3-O-β-D-xylopyranosyl (1''' →2")-β-D-galactopyranoside (major compound) and chlorogenic acid. A validated reversed phase-high-performance liquid chromatography (RP-HPLC) method was developed for the standardization of the bio active ethyl acetate fraction. The calibration curve showed good linearity (r2 > 0.999) within tested ranges. The relative standard deviation of the method was less than 3% for intra- (0.4 - 2.0%) and inter-day (1.9 - 2.8%) assays. Mean recovery of the method was within the range of 98.5 - 102.5%. The minimum detectable concentration of the analyte (LOD) was found to be 0.04 μg/mL. This developed HPLC method was shown to be simple, rapid, precise, reproducible, robust, specifi c, and accurate for quality assessment of the bioactive fraction

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