scholarly journals Development of septal cholinergic neurons in culture: plating density and glial cells modulate effects of NGF on survival, fiber growth, and expression of transmitter-specific enzymes

1988 ◽  
Vol 8 (8) ◽  
pp. 2967-2985 ◽  
Author(s):  
J Hartikka ◽  
F Hefti
Keyword(s):  
Glial Cells ◽  
Cholinergic Neurons ◽  
Fiber Growth ◽  
Plating Density ◽  
Cholinergic Neurons In Culture

scholarly journals Differentiation of Human Embryonic Stem Cells into Neuron, Cholinergic, and Glial Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Kimia Hosseini ◽  
Emilia Lekholm ◽  
Aikeremu Ahemaiti ◽  
Robert Fredriksson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Glial Cells ◽  
Human Embryonic Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Cholinergic Neurons ◽  
Neuronal Cultures ◽  
Short Period

Human embryonic stem cells (hESCs) are pluripotent cells, capable of differentiation into different cellular lineages given the opportunity. Derived from the inner cell mass of blastocysts in early embryonic development, the cell self-renewal ability makes them a great tool for regenerative medicine, and there are different protocols available for maintaining hESCs in their undifferentiated state. In addition, protocols for differentiation into functional human neural stem cells (hNSCs), which have the potential for further differentiation into various neural cell types, are available. However, many protocols are time-consuming and complex and do not always fit for purpose. In this study, we carefully combined, optimized, and developed protocols for differentiation of hESCs into adherent monolayer hNSCs over a short period of time, with the possibility of both expansion and freezing. Moreover, the method details further differentiation into neurons, cholinergic neurons, and glial cells in a simple, single step by step protocol. We performed immunocytochemistry, qPCR, and electrophysiology to examine the expression profile and characteristics of the cells to verify cell lineage. Using presented protocols, the creation of neuronal cultures, cholinergic neurons, and a mixed culture of astrocytes and oligodendrocytes can be completed within a three-week time period.

The Neurotransmitter Noradrenaline Rescues Septal Cholinergic Neurons in Culture from Degeneration Caused by Low-Level Oxidative Stress

2005 ◽  
Vol 67 (6) ◽  
pp. 1882-1891 ◽  
Author(s):  
Sabine Traver ◽  
Bénédicte Salthun-Lassalle ◽  
Marc Marien ◽  
Etienne C. Hirsch ◽  
Francis Colpaert ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cholinergic Neurons ◽  
Low Level ◽  
Cholinergic Neurons In Culture

Cholinergic neurons in culture

2004 ◽  
pp. 405-410
Author(s):  
Menahem Segal ◽  
Varda Greenberger
Keyword(s):  
Cholinergic Neurons ◽  
Cholinergic Neurons In Culture
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