scholarly journals Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids

2015 ◽  
Vol 87 (4) ◽  
pp. 2217-2228 ◽  
Author(s):  
DAIANE M. DRESCH ◽  
TATHIANA E. MASETTO ◽  
SILVANA P.Q. SCALON
Keyword(s):  
Moisture Content ◽  
Silica Gel ◽  
Primary Root ◽  
Dry Mass ◽  
Rna Integrity ◽  
Laboratory Conditions ◽  
Dna And Rna ◽  
Seed Desiccation ◽  
Seedling Length ◽  
Activated Silica Gel

The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1% or less by desiccation using silica gel, and to 17.2% or less by desiccation under laboratory conditions; impairment of the physiological potential of the seeds was observed at these low moisture content levels. The integrity of the seed genomic DNA was not affected after drying seeds in the two methods. However, drying in silica gel to 4.5% moisture content and drying under laboratory conditions to 5.4% moisture content resulted in the loss of seed RNA integrity.

scholarly journals Evaluation of Usefulness of the Designed Laboratory Photobioreactor for Microalgae Cultivation in Controlled Conditions

2016 ◽  
Vol 20 (1) ◽  
pp. 13-22
Author(s):  
Beata Brzychczyk ◽  
Zbigniew Kowalczyk ◽  
Jan Giełżecki
Keyword(s):  
Culture Medium ◽  
Dry Mass ◽  
Biomass Growth ◽  
Microalgae Cultivation ◽  
Freshwater Microalgae ◽  
Laboratory Conditions ◽  
Medium Concentration ◽  
Controlled Conditions ◽  
The Impact

AbstractThe objective of the paper was to analyse the use of the designed photobioreactor for freshwater microalgae cultivation in the controlled laboratory conditions. The work covered the design and construction of photobioreactors (PBR) and setting up comparative cultivations of freshwater microalgae chlorelli vulgaris along with determination of the biomass growth intensity for a varied amount of supplied culture medium. It was found out that the constructed PBR may be used for microalgae cultivation in the controlled conditions. The impact of the culture medium amount on the growth of chlorelli vulgaris was proved. As a result of the increase of culture medium concentration to 30.1-120.4 ml·l−1 of water, dry mass in photobioreactorsincreased respectively from 1.33 g·dm−3 to 4.68 g·dm−3.

scholarly journals Soluble carbohydrates in cereal (wheat, rye, triticale) seed after storage under accelerated ageing conditions

2011 ◽  
Vol 79 (1) ◽  
pp. 21-25
Author(s):  
Agnieszka I. Piotrowicz-Cieślak ◽  
Maciej Niedzielski ◽  
Dariusz J. Michalczyk ◽  
Wiesław Łuczak ◽  
Barbara Adomas
Keyword(s):  
Moisture Content ◽  
Spring Wheat ◽  
Soluble Sugars ◽  
Dry Mass ◽  
Soluble Carbohydrates ◽  
Accelerated Ageing ◽  
Winter Rye ◽  
Seed Moisture Content ◽  
Winter Triticale ◽  
Seed Moisture

Germinability and the content of soluble carbohydrates were analysed in cereal seed (winter rye, cv. Warko; spring wheat, cv. Santa; hexaploid winter triticale, cv. Fidelio and cv. Woltario). Seed moisture content (mc) was equilibrated over silica gel to 0.08 g H<sub>2</sub>O/g dry mass and stored in a desiccator at 20<sup>o</sup>C for up to 205 weeks or were equilibrated to mc 0.06, 0.08 or 0.10 g H<sub>2</sub>O/g dm and subjected to artificial aging at 35<sup>o</sup>C in air-tight laminated aluminium foil packages for 205 weeks. It was shown that the rate of seed aging depended on the species and seed moisture content. The fastest decrease of germinability upon storage was observed in seed with the highest moisture level. Complete germinability loss for winter rye, winter triticale cv. Fidelio, winter triticale cv. Woltario and spring wheat seed with mc 0.10 g H<sub>2</sub>O/g dm<sup>3</sup> occurred after 81, 81, 101 and 133 weeks, respectively. Fructose, glucose, galactose, myo-inositol, sucrose, galactinol, raffinose, stachyose and verbascose were the main soluble carbohydrates found in the seed. The obtained data on the contents of specific sugars and the composition of soluble sugars fraction in seed of rye, wheat and triticale did not corroborate any profound effect of reducing sugars, sucrose and oligosaccharides on seed longevity.

scholarly journals The Technological Investigations Of Manufacturing Of Energy Chips

2015 ◽  
Vol 1 ◽  
pp. 220
Author(s):  
Ziedonis Miklašēvičs
Keyword(s):  
Moisture Content ◽  
Dry Mass ◽  
Quality Parameters ◽  
Heating Value ◽  
Long Term Storage ◽  
Lower Heating Value ◽  
Term Storage ◽  
Lower Heating

The methodology in Latvia forest industry provide to determine the quality of energy chips only in long- term storage places before selling. Due to the lack of hard empirical data about the quality parameters of energy chips in different phases of manufacturing process, this research paper consists of: - the identification and analyses of the factors that influenced the values of energy chips quality features such as: bulk density, moisture content, ash content, higher and lower heating value according to actual moisture content and per dry mass of the chips; - the methodology for determination the quality parameters of energy chips by analysis the wood moisture content and by choice the method of the manufacturing of energy chips.

scholarly journals Assessment of Seed Vigor Tests for Crambe abyssinica

2018 ◽  
Vol 10 (12) ◽  
pp. 527
Author(s):  
Tássia Fernanda Santos Neri Soares ◽  
Bruno Antônio Lemos de Freitas ◽  
José Geraldo de Araújo Ferreira Filho ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
Camila Andrade Fialho ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Water Stress ◽  
Stress Test ◽  
Primary Root ◽  
Cold Test ◽  
Seed Vigor ◽  
Stress Tests ◽  
Germination Test ◽  
Crambe Abyssinica ◽  
Seedling Length

Vigor tests are important mainly to identify differences associated to lots performance in field conditions. The objective of this study was to define the most appropriate tests to evaluate the crambe (Crambe abyssinica) seeds vigor. Seven seed lots of crambe were subjected to the standard germination (on the first and last count), germination speed index (GSI), primary root protrusion speed index (PRPSI), seedling length dry and weight seedling, water stress test using PEG 6000 at -0.2 MPa, electrical conductivity and cold test without soil. Crambe lots studied had germination percentages varying from 54 to 80.5%. The GSI, on the first count of water stress and electrical conductivity test ranked lots between two vigor classes The PRPSI, seedling length, seedling dry weight and last count of water stress tests were less sensitive to evaluate vigor for crambe seed. The cold test without soil and the first count of standard germination test were capable to dishtinguish lots more efficiently than the standard germination test on last count by ranking four of the seven seed lots studied.

scholarly journals Yield and physiological quality of wheat seeds after desiccation with different herbicides

2017 ◽  
Vol 39 (3) ◽  
pp. 254-261 ◽  
Author(s):  
Fábio Henrique Krenchinski ◽  
Victor José Salomão Cesco ◽  
Danilo Morilha Rodrigues ◽  
Vinicius Gabriel Caneppele Pereira ◽  
Alfredo Junior Paiola Albrecht ◽  
...  
Keyword(s):  
Seed Quality ◽  
Block Design ◽  
Dry Mass ◽  
Wheat Crop ◽  
Glufosinate Ammonium ◽  
Randomized Block Design ◽  
Seedling Length ◽  
Physiological Quality ◽  
Wheat Seeds

Abstract: Some desiccation techniques for harvesting wheat may affect seed quality. The objective of this study was to evaluate the effect of different desiccant herbicides applied to the wheat crop and their effects on the production and seed quality. The experiment was conducted with cultivar COODETEC 150 (CD 150) in two sites in Palotina (Paraná state, Brazil) with a randomized block design consisting of 7 treatments (desiccation herbicides) and 4 replications. Desiccation was realized when 50% of the plants were in stage 80 with seeds in powdery or mass consistency. The variables analyzed were percentage of moisture content after harvest, Yield, hectoliter weight, mass of 100 seeds, vigor, germination, fresh mass of seedlings, dry mass of seedlings and root length. The herbicides carfentrazone-ethyl and clethodim were the ones that promoted greatest reduction in vigor. The herbicide paraquat caused reduction in seedling length. There was a reduction in the mass of 100 seeds for glufosinate-ammonium, clethodim, diquat and carfentrazone-ethyl. Glufosinate-ammonium, paraquat, glyphosate, clethodim and diquat caused reduction in yield. Desiccation of Cultivar CD 150 at stage 80 is not recommended, since plant Yield and physiological seed parameters were adversely affected by herbicides.

scholarly journals Effects of Aluminum and pH on Germination of Echium plantagineum L.

2020 ◽  
Vol 8 (1) ◽  
pp. 313
Author(s):  
Rodrigo Roso ◽  
Ubirajara Russi Nunes ◽  
Caren Alessandra Müller ◽  
Juçara Terezinha Paranhos ◽  
Sidinei José Lopes ◽  
...  
Keyword(s):  
Aerial Part ◽  
Aluminum Sulfate ◽  
Growth Parameters ◽  
Primary Root ◽  
Dry Mass ◽  
The Third ◽  
Sulfate Solutions ◽  
Randomized Design ◽  
Primary Root Length ◽  
Growth Of Seedlings

The objetive of this study was to identify the effects of aluminum concentrations and pH levels of solution on germination of Echium plantagineum L. seeds. Three different experiments were carried out in completely randomized design with four repetitions. In the first experiment, we used aluminum sulfate solutions in concentrations of 0.0; 0.3; 0.6; 1.0; 2.0; 3.0; 4.0; 5.0; 6.0 and 7.0 cmolc L-1 and in the second experiment, solutions with different pH, 3.0; 4.0; 5.0; 6.0; 7.0; 8.0; 9.0 and 10.0. In the third experiment, we carried out a factorial (4x4) with aluminum sulfate solutions (0.0; 2.0; 4.0 and 6.0 cmolc L-1) and pH (4.0; 5.0; 6.0 and 7.0). It was evaluated the germination of Echium plantagineum L. at four and 14 days after seeding (DAS), germination speed index, primary root length, aerial part and dry mass of seedlings. The presence of aluminum reduced the germination by 27 and 40% at four and 14 DAS, respectively, in concentrations superior to 3.0 cmolc L-1. The three growth parameters presented linear reduction with the increase of aluminum concentrations. In the second experiment, the solutions with pH of 3.0 and 10.0 provided increases in germination, length of root and aerial part, and little influence in the dry mass of seedlings. In the third experiment, there was significant interaction between the aluminum concentrations and pH levels of substrate. The presence of aluminum in the substrate presented toxic effect on germination of seeds, length of seedlings and dry mass. The pH of the solution has little effect in germination of seeds and in the growth of seedlings of E. plantagineum.

De-coupling seasonal changes in water content and dry matter to predict live conifer foliar moisture content

2014 ◽  
Vol 23 (4) ◽  
pp. 480 ◽  
Author(s):  
W. Matt Jolly ◽  
Ann M. Hadlow ◽  
Kathleen Huguet
Keyword(s):  
Water Stress ◽  
Moisture Content ◽  
Water Content ◽  
Seasonal Variations ◽  
Seasonal Changes ◽  
Dry Matter ◽  
Water Mass ◽  
Water Status ◽  
Dry Mass ◽  
Dry Matter Partitioning

Live foliar moisture content (LFMC) significantly influences wildland fire behaviour. However, characterising variations in LFMC is difficult because both foliar mass and dry mass can change throughout the season. Here we quantify the seasonal changes in both plant water status and dry matter partitioning. We collected new and old foliar samples from Pinus contorta for two growing seasons and quantified their LFMC, relative water content (RWC) and dry matter chemistry. LFMC quantifies the amount of water per unit fuel dry weight whereas RWC quantifies the amount of water in the fuel relative to how much water the fuel can hold at saturation. RWC is generally a better indicator of water stress than is LFMC. We separated water mass from dry mass for each sample and we attempted to best explain the seasonal variations in each using our measured physiochemical variables. We found that RWC explained 59% of variation in foliar water mass. Additionally, foliar starch, sugar and crude fat content explained 87% of the variation in seasonal dry mass changes. These two models combined explained 85% of the seasonal variations in LFMC. These results demonstrate that changes to dry matter exert a stronger control on seasonal LFMC dynamics than actual changes in water content, and they challenge the assumption that LFMC variations are strongly related to water stress. This methodology could be applied across a range of plant functional types to better understand the factors that drive seasonal changes in LFMC and live fuel flammability.

Optimization of Chromatographic Conditions for the Separation of p,p´-DDE from Aroclor 1254 on Silica, Using Azulene as Indicator

1980 ◽  
Vol 63 (5) ◽  
pp. 959-964
Author(s):  
Donald A Griffin ◽  
Anna B Marin ◽  
Max L Deinzer
Keyword(s):  
Silica Gel ◽  
Load Capacity ◽  
Separation System ◽  
Aroclor 1254 ◽  
Aromatic Content ◽  
Pesticide Load ◽  
Activated Silica Gel ◽  
Visual Marker

Abstract Aroclor 1254 is separated from p,p´-DDE on a fully activated silica gel column with the aid of azulene as a visual marker. The azulene is useful in determining the best cutpoint for separating these compounds and has been valuable in characterizing the effects of several parameters of the separation system, such as the aromatic content of the eluting solvent, adsorbent activity, and lipid and pesticide load capacity. Several brands of silica are compared for their ability to separate these compounds.

scholarly journals Temperatures and substrates for germination and vigor of Erythroxylum pauferrense Plowman seeds

2019 ◽  
Vol 41 ◽  
pp. e46030
Author(s):  
João Everthon da Silva Ribeiro ◽  
Arliston Pereira Leite ◽  
Jackson Silva Nóbrega ◽  
Edna Ursulino Alves ◽  
Riselane de Lucena Alcântara Bruno ◽  
...  
Keyword(s):  
Dry Mass ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Randomized Design ◽  
Temperature Regimes ◽  
Seedling Length ◽  
Physiological Quality ◽  
Mean Germination Time ◽  
Completely Randomized Design ◽  
Understory Plant

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.

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