scholarly journals Tissue Response after Subcutaneous Implantation of Different Glass Ionomer-Based Cements

2019 ◽  
Vol 30 (6) ◽  
pp. 599-606
Author(s):  
Carolina Maschietto Pucinelli ◽  
Raquel Assed Bezerra da Silva ◽  
Luã Lopes Borges ◽  
Alberto Tadeu do Nascimento Borges ◽  
Paulo Nelson-Filho ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Collagen Fiber ◽  
Fiber Formation ◽  
Tissue Response ◽  
Control Group ◽  
Advanced Stage ◽  
Glass Ionomer ◽  
Granulomatous Tissue ◽  
Subcutaneous Connective Tissue

Abstract The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice after implantation of different glass ionomer-based cements (EQUIA® Forte Fil, EQUIA® Fil and Ketac™ Universal Aplicap™). Eighty-seven isogenic BALB/c mice were allocated in 12 groups, 9 were considered as experimental groups (Ketac, E. Fil and E. Forte at 7, 21 and 63 days) and 3 controls (empty polyethylene tubes at 7, 21 and 63 days). After the experimental periods, the subcutaneous connective tissue surrounding the implanted material was removed and subjected to histotechnical processing and staining with hematoxylin and eosin. A histopathological description of the tissue reaction surrounding each material and a semi-quantitative analysis of collagen fiber formation and inflammatory infiltrate were performed. Additionally, the thickness of the granulomatous tissue in contact with each material was measured. Data were analyzed statistically (α=0.05) by the Kruskal-Wallis test, followed by Dunn post-test. Initially, the collagen fiber formation was not different among all the tested materials (p>0.05) but was different at 21 days with the control group presenting the most advanced stage of collagen fiber formation. At 63 days, EQUIA® Forte Fil group showed the most advanced stage of collagen fiber formation, compared to EQUIA® Fil group (p<0.05). The inflammatory infiltrate was not different among the tested materials in any experimental period (p>0.05). The thickness of the granulomatous tissue was greater in the E. Forte group, compared to control in all periods. All glass ionomer-based cements showed tissue compatibility, according to the evaluated parameters.

scholarly journals Subcutaneous connective tissue response to primary root canal filling materials

2011 ◽  
Vol 22 (3) ◽  
pp. 203-211 ◽  
Author(s):  
Alexandra Mussolino de Queiroz ◽  
Sada Assed ◽  
Alberto Consolaro ◽  
Paulo Nelson-Filho ◽  
Mario Roberto Leonardo ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Quantitative Analysis ◽  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Collagen Fiber ◽  
Fiber Formation ◽  
Tissue Response ◽  
Filling Materials ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue

This study evaluated the response of the subcutaneous connective tissue of BALB/c mice to root filling materials indicated for primary teeth: zinc oxide/eugenol cement (ZOE), Calen paste thickened with zinc oxide (Calen/ZO) and Sealapex sealer. The mice (n=102) received polyethylene tube implants with the materials, thereby forming 11 groups, as follows: I, II, III: Calen/ZO for 7, 21 and 63 days, respectively; IV, V, VI: Sealapex for 7, 21 and 63 days, respectively; VII, VIII, IX: ZOE for 7, 21 and 63 days, respectively; X and XI: empty tube for 7 and 21 days, respectively. The biopsied tissues were submitted to histological analysis (descriptive analysis and semi-quantitative analysis using a scoring system for collagen fiber formation, tissue thickness and inflammatory infiltrate). A quantitative analysis was performed by measuring the area and thickness of the granulomatous reactionary tissue (GRT). Data were analyzed by Kruskal-Wallis, ANOVA and Tukey's post-hoc tests (?=0.05). There was no significant difference (p>0.05) among the materials with respect to collagen fiber formation or GRT thickness. However, Calen/ZO produced the least severe inflammatory infiltrate (p<0.05). The area of the GRT was significantly smaller (p<0.05) for Calen/ZO and Sealapex. In conclusion, Calen/ZO presented the best tissue reaction, followed by Sealapex and ZOE.

scholarly journals Subcutaneous tissue response of isogenic mice to calcium hydroxide-based pastes with chlorhexidine

2009 ◽  
Vol 20 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Raquel Assed Bezerra da Silva ◽  
Sada Assed ◽  
Paulo Nelson-Filho ◽  
Léa Assed Bezerra da Silva ◽  
Alberto Consolaro
Keyword(s):  
Quantitative Analysis ◽  
Connective Tissue ◽  
Calcium Hydroxide ◽  
Inflammatory Infiltrate ◽  
Subcutaneous Tissue ◽  
Fibrous Tissue ◽  
Fiber Formation ◽  
Tissue Response ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue

This study was evaluated the response of subcutaneous connective tissue of isogenic mice to calcium hydroxide-based pastes with chlorhexidine digluconate (CHX). Seventy isogenic male BALB/c mice aged 6-8 weeks and weighing 15-20 g were randomly assigned to 8 groups. The animals received polyethylene tube implants as follows: Groups I, II, and III (n=10) - Calen® paste mixed with 0.4% CHX (experimental paste; Calen/CHX) for 7, 21, and 63 days, respectively; Groups IV, V, and VI (n=10) - UltraCal™ paste mixed with 2% CHX (experimental paste supplied by Ultradent Products Inc.; Ultracal/CHX) for 7, 21, and 63 days, respectively; and Groups VII and VIII (n=5): empty tube for 7 and 21 days, respectively. At the end of the experimental periods, the implants were removed together with the surrounding tissues (skin and subcutaneous connective tissue). The biopsied tissues were subjected to routine processing for histological analysis. Using a descriptive analysis and a four-point (0-3) scoring system, the following criteria were considered for qualitative and quantitative analysis of the tissue around the implanted materials: collagen fiber formation, tissue thickness and inflammatory infiltrate. A quantitative analysis was performed by measuring the thickness (µm), area (µm²) and perimeter (µm) of the reactionary granulomatous tissue formed at the tube ends. Data were analyzed statistically by the Kruskal-Wallis test and Dunn's post-test (α=0.05). Calen/CHX showed biocompatibility with the subcutaneous and reactionary tissues, with areas of discrete fibrosis and normal conjunctive fibrous tissue, though without statistically significant difference (p>0.05) from the control groups. In Groups I to III, there was a predominance of score 1, while in Groups IV to VI scores 2 and 3 predominated for all analyzed parameters. UltraCal/CHX, on the other hand, induced the formation of an inflammatory infiltrate and abundant exudate, suggesting a persistent residual aggression from the material, even 63 days after implant placement. In conclusion, the Calen paste mixed with 0.4% CHX allowed an adequate tissue response, whereas the UltraCal paste mixed with 2% CHX showed unsatisfactory results.

scholarly journals A Comparative Study of Biocompatibility in Rat Connective Tissue of a New Mineral Trioxide Compound (Theracal) versus MTA and a Bioactive G3 Glass

2021 ◽  
Vol 10 (12) ◽  
pp. 2536
Author(s):  
Jesús Mena-Álvarez ◽  
Cristina Rico-Romano ◽  
Carlos Gutiérrez-Ortega ◽  
Pablo Arias-Sanz ◽  
Javier Castro-Urda
Keyword(s):  
Connective Tissue ◽  
Inflammatory Reaction ◽  
Direct Contact ◽  
Wistar Rats ◽  
Tissue Response ◽  
New Mineral ◽  
Exact Test ◽  
Dorsal Area ◽  
Subcutaneous Connective Tissue ◽  
Hematoxylin Eosin

The aim of this paper was to assess the differences in tissue response to implantation during 15, 30 and 45 days in the subcutaneous connective tissue of Wistar rats from three biomaterials: Angelus MTA®, Theracal LC®, and Angelus MTA® to which 25% bioglass G3 was added. Twenty-four Wistar rats were used, the materials were inserted into the rat’s dorsal area in silicone tubes 5 mm long by 1.5 mm diameter. Histological reaction was assessed at 15, 30, and 45 days after implantation. They were then stained with hematoxylin eosin and evaluated by two observers. Data were analyzed using Fisher’s exact test and Mann–Whitney’s U test was used to determine the association between variables. Angelus MTA induced the formation of dystrophic calcifications twice as much as Theracal LC (p < 0.05). The addition of G3 did not affect the greater or lesser occurrence of calcifications (p > 0.05). Theracal LC and MTA plus G3 caused an inflammatory reaction, which was chronic at 15 days and decreased in intensity, almost disappearing after 45 days. Theracal LC, as well as Angelus MTA plus G3, were well tolerated when implanted in the subcutaneous connective tissue of rat. Histologically, no inconvenience was found for the use by direct contact of Theracal LC, and the mixture of MTA with 25% bioactive glass G3, in the tissue of Wistar rats.

scholarly journals Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue

2005 ◽  
Vol 13 (4) ◽  
pp. 382-386 ◽  
Author(s):  
Willian Fernando Zambuzzi ◽  
Rodrigo Cardoso de Oliveira ◽  
Danilo Alanis ◽  
Renato Menezes ◽  
Ariadne Letra ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Plasma Cells ◽  
Subcutaneous Tissue ◽  
Repair Process ◽  
Giant Cells ◽  
Tissue Response ◽  
Control Group ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis ◽  
Severe Adverse Reactions

The tissue response to porous bovine anorganic bone implanted in rat connective tissue was evaluated by subjective light microscopy analysis. Forty rats were divided into two groups: control (empty collagen capsules) and test (collagen capsule filled with 0.1g biomaterial) and killed 10, 20, 30 and 60 days after implantation. At 10 days, intense chronic inflammatory infiltrate consisting mainly of macrophages and inflammatory multinucleated giant cells (IMGC) was observed. Neutrophils, plasma cells and lymphocytes were present in discrete amounts and slowly disappeared along the repair process. Porosity of the material was filled by reaction connective tissue exhibiting IMGC. The fibrosis was more intense after 60 days and clearly higher than the control group. Thus, the material did not cause any severe adverse reactions and did not stimulate the immune system. Based on the results it could be concluded that deproteinized bovine cancelous bone was well tolerated by rat connective tissue.

scholarly journals Rat subcutaneous tissue response to macrogranular porous anorganic bovine bone graft

2006 ◽  
Vol 17 (4) ◽  
pp. 274-278 ◽  
Author(s):  
Willian Fernando Zambuzzi ◽  
Rodrigo Cardoso de Oliveira ◽  
Felipe Ladeira Pereira ◽  
Tânia Mary Cestari ◽  
Rumio Taga ◽  
...  
Keyword(s):  
Bone Graft ◽  
Inflammatory Infiltrate ◽  
Plasma Cells ◽  
Subcutaneous Tissue ◽  
Test Group ◽  
Tissue Response ◽  
Test Material ◽  
Control Group ◽  
Bovine Bone ◽  
Body Type

The ideal bone graft must present biocompatibility, osteoconductive and osteoinductive properties, resistance and plasticity. Xenogenic grafts of bovine cancellous bone origin are particularly interesting due to their biologically designed porous structure that enhance both cellular and vascular invasion. The purpose of this study was to evaluate the tissue response induced by bovine macrogranular porous anorganic bone implanted in rat subcutaneous tissue. Forty rats were assigned to 2 groups, as follows: the control group received empty collagen capsules and the test group received subcutaneous implants of the test material. Samples were collected after 10, 20, 30 and 60 days and processed histologically. Histological analysis showed at 10 days a granulomatous inflammatory infiltrate, rich in multinucleated giant cells and free of lymphocytes or plasma cells, similarly to mineralized allograft implanted in rat subcutaneous. In later periods, there was a significant decrease in the inflammatory infiltrate and an increase in fibrosis around graft particles. In conclusion, the test material induced a foreign body-type granuloma with subsequent fibrosis around the graft particles implanted in rat subcutaneous and did not elicit any immune response, thus being considered biocompatible.

scholarly journals Tissue toxicity of root canal sealers

2004 ◽  
Vol 51 (3) ◽  
pp. 136-141 ◽  
Author(s):  
Mirjana Vujaskovic ◽  
Dragoljub Bacetic
Keyword(s):  
Connective Tissue ◽  
Root Canal ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Control Material ◽  
Tissue Sections ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions ◽  
Root Canal Sealers ◽  
Observation Period

The aim of this study was to evaluate tissue response to root canal sealers Tubuliseal and Sealapex. The sealers were freshly mixed and injected in the dorsal subcutaneous connective tissue of 12 Wistar rats.The observation periods were 7 days, 21days and 60 days. Four operative areas were formed ( 2 for test sealers, Tubliseal or Sealapex and 2 for control material) on each animal. Tissue sections were taken from selected sites. Each section included skin, subcutaneous connective tissue and underlying muscle tissue.All blocks were processed with the use of standardized histological procedures.The tissue reactions were studied under light microscopy. Different grades of tissue reaction to the tested materials were recorded as mild, moderate or severe inflammation. After seven days both root canal sealers showed severe inflammatory reaction of connective tissue in experimental animals.Tubuliseal caused prolonged moderate and mild inflammation. Sealapex caused mild inflammation which diminished at the end of the observation period. The results of this study demonstrated that Sealapex was better tolerated by tissue than Tubliseal.

scholarly journals Comparison of Rat Connective Tissue Response to BioMTA, Angelus MTA, and Root MTA

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Marzieh Alimohammadi ◽  
Sina Mirzaee-Rad ◽  
Farideh Feizi ◽  
Hadi Shirzad Juybari ◽  
Akam Saeidi ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Wistar Rats ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Control Group ◽  
Capsule Formation ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Difference ◽  
Fibrous Capsule Formation

Due to the widespread use of MTA in dentistry and various brands of this product, we decided to compare the three brands available in the country market by their biocompatibility. We divided 20 male Wistar rats into four groups. After local anesthesia and washing, we made two incisions on both sides (4 incisions in total). The experimental groups were Angelus MTA (Angelus, Brazil), BioMTA (CERKAMED, Poland), Root MTA (Dr. Lotfi, Tabriz, Iran), and the control group. The resulting paste was placed in a tube and implanted subcutaneously into male Wistar rats. Wistar rats were sacrificed 7, 15, 30, and 60 days later, with high anesthetic doses. The sample implanted in 10% formalin was stabilized after tissue processing and H&E staining under a microscope. The inflammatory reaction in the tissues received different scores at the beginning of the tube opening. BioMTA had the highest inflammatory response among the groups, but the difference was not statistically significant ( p  > 0.05). Also, there was no significant difference between the groups’ granulation and calcification ( p  < 0.05). There was a significant difference between BioMTA, Angelus MTA, Root MTA, and control groups in fibrous capsule formation ( p  < 0.05). Angelus MTA showed the lowest mean fibrous capsule formation in all periods. The effects of Angelus MTA, Root MTA, and BioMTA on connective tissue were investigated and compared. According to this study, these materials have good biocompatibility. According to the findings and statistical analysis, Angelus MTA has the most biocompatibility.

scholarly journals Reaction of Rat Subcutaneous Connective Tissue to Resin Composites Polymerized with Different Light Curing Units and Different Lightening Methods

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Atiyeh Feiz ◽  
Farahnaz Arbabzadeh Zavareh ◽  
Seyed Mohammad Razavi ◽  
Hamid Badrian ◽  
Sepideh Dolatyar ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Resin Composite ◽  
Control Group ◽  
Albino Rats ◽  
Resin Composites ◽  
Significant Difference ◽  
Light Curing ◽  
Full Power ◽  
Subcutaneous Connective Tissue

The aim of the study was to determine and compare the reaction of rat subcutaneous connective tissue to resin composites polymerized with different lights curing and lightening methods. In this in vivo study, 20 mature Wister Albino rats were used. The composite discs, 4 mm in diameter and 2 mm thick, were cured by QTH or LED light curing units with 4 different lightning methods (full power QTH, full power LED, pulse LED, and ramp LED). Five resin composite discs were implanted in each rat, so that 4 of 5 discs for implantation of cured composite discs differently and central one as control without implantation. After sacrificing at 7, 14, 30, and 60 days the inflammatory grade, fibrosis, and necrosis were determined. Freedman and Cochran tests were used to analyze the data using SPSS software ver. 15. The results of the study showed significant differences in inflammation grade and fibrosis among control group and 4 experimental groups at day 14 (P<0.05). In necrosis, there was no significant difference among 4 groups in different times (P>0.05). In conclusion, neither the type of light curing units (LED or QTH) nor the lightening methods can affect the grade of inflammatory reaction.

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