scholarly journals Subcutaneous connective tissue response to primary root canal filling materials

2011 ◽  
Vol 22 (3) ◽  
pp. 203-211 ◽  
Author(s):  
Alexandra Mussolino de Queiroz ◽  
Sada Assed ◽  
Alberto Consolaro ◽  
Paulo Nelson-Filho ◽  
Mario Roberto Leonardo ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Quantitative Analysis ◽  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Collagen Fiber ◽  
Fiber Formation ◽  
Tissue Response ◽  
Filling Materials ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue

This study evaluated the response of the subcutaneous connective tissue of BALB/c mice to root filling materials indicated for primary teeth: zinc oxide/eugenol cement (ZOE), Calen paste thickened with zinc oxide (Calen/ZO) and Sealapex sealer. The mice (n=102) received polyethylene tube implants with the materials, thereby forming 11 groups, as follows: I, II, III: Calen/ZO for 7, 21 and 63 days, respectively; IV, V, VI: Sealapex for 7, 21 and 63 days, respectively; VII, VIII, IX: ZOE for 7, 21 and 63 days, respectively; X and XI: empty tube for 7 and 21 days, respectively. The biopsied tissues were submitted to histological analysis (descriptive analysis and semi-quantitative analysis using a scoring system for collagen fiber formation, tissue thickness and inflammatory infiltrate). A quantitative analysis was performed by measuring the area and thickness of the granulomatous reactionary tissue (GRT). Data were analyzed by Kruskal-Wallis, ANOVA and Tukey's post-hoc tests (?=0.05). There was no significant difference (p>0.05) among the materials with respect to collagen fiber formation or GRT thickness. However, Calen/ZO produced the least severe inflammatory infiltrate (p<0.05). The area of the GRT was significantly smaller (p<0.05) for Calen/ZO and Sealapex. In conclusion, Calen/ZO presented the best tissue reaction, followed by Sealapex and ZOE.

scholarly journals Subcutaneous tissue response of isogenic mice to calcium hydroxide-based pastes with chlorhexidine

2009 ◽  
Vol 20 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Raquel Assed Bezerra da Silva ◽  
Sada Assed ◽  
Paulo Nelson-Filho ◽  
Léa Assed Bezerra da Silva ◽  
Alberto Consolaro
Keyword(s):  
Quantitative Analysis ◽  
Connective Tissue ◽  
Calcium Hydroxide ◽  
Inflammatory Infiltrate ◽  
Subcutaneous Tissue ◽  
Fibrous Tissue ◽  
Fiber Formation ◽  
Tissue Response ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue

This study was evaluated the response of subcutaneous connective tissue of isogenic mice to calcium hydroxide-based pastes with chlorhexidine digluconate (CHX). Seventy isogenic male BALB/c mice aged 6-8 weeks and weighing 15-20 g were randomly assigned to 8 groups. The animals received polyethylene tube implants as follows: Groups I, II, and III (n=10) - Calen® paste mixed with 0.4% CHX (experimental paste; Calen/CHX) for 7, 21, and 63 days, respectively; Groups IV, V, and VI (n=10) - UltraCal™ paste mixed with 2% CHX (experimental paste supplied by Ultradent Products Inc.; Ultracal/CHX) for 7, 21, and 63 days, respectively; and Groups VII and VIII (n=5): empty tube for 7 and 21 days, respectively. At the end of the experimental periods, the implants were removed together with the surrounding tissues (skin and subcutaneous connective tissue). The biopsied tissues were subjected to routine processing for histological analysis. Using a descriptive analysis and a four-point (0-3) scoring system, the following criteria were considered for qualitative and quantitative analysis of the tissue around the implanted materials: collagen fiber formation, tissue thickness and inflammatory infiltrate. A quantitative analysis was performed by measuring the thickness (µm), area (µm²) and perimeter (µm) of the reactionary granulomatous tissue formed at the tube ends. Data were analyzed statistically by the Kruskal-Wallis test and Dunn's post-test (α=0.05). Calen/CHX showed biocompatibility with the subcutaneous and reactionary tissues, with areas of discrete fibrosis and normal conjunctive fibrous tissue, though without statistically significant difference (p>0.05) from the control groups. In Groups I to III, there was a predominance of score 1, while in Groups IV to VI scores 2 and 3 predominated for all analyzed parameters. UltraCal/CHX, on the other hand, induced the formation of an inflammatory infiltrate and abundant exudate, suggesting a persistent residual aggression from the material, even 63 days after implant placement. In conclusion, the Calen paste mixed with 0.4% CHX allowed an adequate tissue response, whereas the UltraCal paste mixed with 2% CHX showed unsatisfactory results.

scholarly journals Tissue Response after Subcutaneous Implantation of Different Glass Ionomer-Based Cements

2019 ◽  
Vol 30 (6) ◽  
pp. 599-606
Author(s):  
Carolina Maschietto Pucinelli ◽  
Raquel Assed Bezerra da Silva ◽  
Luã Lopes Borges ◽  
Alberto Tadeu do Nascimento Borges ◽  
Paulo Nelson-Filho ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Collagen Fiber ◽  
Fiber Formation ◽  
Tissue Response ◽  
Control Group ◽  
Advanced Stage ◽  
Glass Ionomer ◽  
Granulomatous Tissue ◽  
Subcutaneous Connective Tissue

Abstract The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice after implantation of different glass ionomer-based cements (EQUIA® Forte Fil, EQUIA® Fil and Ketac™ Universal Aplicap™). Eighty-seven isogenic BALB/c mice were allocated in 12 groups, 9 were considered as experimental groups (Ketac, E. Fil and E. Forte at 7, 21 and 63 days) and 3 controls (empty polyethylene tubes at 7, 21 and 63 days). After the experimental periods, the subcutaneous connective tissue surrounding the implanted material was removed and subjected to histotechnical processing and staining with hematoxylin and eosin. A histopathological description of the tissue reaction surrounding each material and a semi-quantitative analysis of collagen fiber formation and inflammatory infiltrate were performed. Additionally, the thickness of the granulomatous tissue in contact with each material was measured. Data were analyzed statistically (α=0.05) by the Kruskal-Wallis test, followed by Dunn post-test. Initially, the collagen fiber formation was not different among all the tested materials (p>0.05) but was different at 21 days with the control group presenting the most advanced stage of collagen fiber formation. At 63 days, EQUIA® Forte Fil group showed the most advanced stage of collagen fiber formation, compared to EQUIA® Fil group (p<0.05). The inflammatory infiltrate was not different among the tested materials in any experimental period (p>0.05). The thickness of the granulomatous tissue was greater in the E. Forte group, compared to control in all periods. All glass ionomer-based cements showed tissue compatibility, according to the evaluated parameters.

Tissue Reaction to Subcutaneous Implantation of SuperEBA (Reinforced Zinc Oxide Cement) Used as Root-End Filling Material: A Histological Study in Rats

2016 ◽  
Vol 695 ◽  
pp. 247-251
Author(s):  
Alexandru Andrei Iliescu ◽  
Cristian Marian Petcu ◽  
Ileana Cristiana Petcu ◽  
Irina Maria Gheorghiu ◽  
Andrei Iliescu ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Histological Study ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Successful Outcome ◽  
Filling Material ◽  
Clinical Value ◽  
Filling Materials ◽  
Retrograde Filling ◽  
Specific Tissue

The retrograde filling is a critical step to a successful outcome of the endodontic surgery. Despite the progress in the technology of novel root-end filling materials, zinc oxide-eugenol cement superEBA is still preserving its clinical value on long-term basis. The study aimed to reconsider the tissue response to the initial irritating effect of this material. Silicon tubes filled with superEBA were subcutaneously implanted for 120 days in white Wistar rats which were afterwards sacrificed. The connective tissue surrounding the superEBA implants revealed fibroblast proliferation and a definite reparatory process without inflammatory reaction. A non-specific tissue healing in progress around the implants, without calcifications, necrosis, and apoptosis was also described after 4 months. SuperEBA proved on animal model that its cytotoxicity is reducing gradually in time until no adverse reaction is observed. The reduced content in eugenol compared to other surgical zinc oxide cements and the benefic effect of o-ethoxybenzoic acid are the support to reconsider SuperEBA as a biocompatible retrograde filling material.

scholarly journals Characterization of Cytokines and Proliferation Marker Ki67 in Cleft Affected Lip Tissue

Medicina ◽  
2019 ◽  
Vol 55 (9) ◽  
pp. 518 ◽  
Author(s):  
Pilmane ◽  
Sidhoma ◽  
Akota ◽  
Kazoka
Keyword(s):  
Immune Response ◽  
Connective Tissue ◽  
Cleft Lip ◽  
Cellular Proliferation ◽  
Tissue Response ◽  
Compensatory Mechanism ◽  
Strong Positive Correlation ◽  
Proliferation Marker ◽  
Significant Difference ◽  
Anti Inflammatory

Background and objectives: Cleft lip palate takes the second place among all anomalies. The complex appearance of cytokines and proliferation markers has still not been clarified despite their possible crucial role in cleft tissue. Therefore, the aim of work was the detection of appearance of pro- and anti-inflammatory cytokines and proliferation marker Ki67, and their inter-correlations in cleft affected lip (CAL). Materials and Methods: The lip material was obtained from 16 children aged before primary dentition during plastic surgery. Control was obtained from 7 non-CAL oral tissue. Tissues were stained for IL-1, IL-4, IL-6, IL-8, IL-10 and Ki67 immunohistochemically. Non-parametric statistic, Mann–Whitney and Spearman’s coefficient were used. Results: All cytokines positive cells were observed more into the epithelium. Statistically significant difference was seen between epithelial IL-1, IL-10, IL-8 and Ki67 positive cells and IL-10-, IL-4-containing connective tissue cells in comparison to the control. Strong positive correlation was detected in CAL epithelium between IL-10 and IL-8, IL-10 and IL-4, IL-10 and IL-1, IL-1 and IL-8, IL-1 and IL-4, IL-4 and IL-8, IL-8 and Ki67, IL-10 and Ki67, but moderate—in connective tissue between IL-1 and IL-10, IL-1 and IL-4. Conclusion: The CAL epithelium is the main source for the interleukins. Rich similar expression of IL-1 and IL-10 suggests the balance between pro-and anti-inflammatory tissue response on basis of dysregulated tissue homeostasis (increase of IL-8). The correlations between the different ILs -1, -4, -8, -10 in CAL epithelium seem to indicate the self-protection compensatory mechanism for intensification of local inflammatory-immune response without involvement of IL-6. The correlations between Ki67 and cytokines indicate the involvement of IL-8 and IL-10 in stimulation of cellular proliferation. IL-4 and IL-10 expression from CAL connective tissue simultaneously to IL-1, IL-4 and IL-10 inter-correlations there suggests the intensification of local immune response regulated probably by main pro-inflammatory cytokine—IL-1.

scholarly journals A Comparative Study of Biocompatibility in Rat Connective Tissue of a New Mineral Trioxide Compound (Theracal) versus MTA and a Bioactive G3 Glass

2021 ◽  
Vol 10 (12) ◽  
pp. 2536
Author(s):  
Jesús Mena-Álvarez ◽  
Cristina Rico-Romano ◽  
Carlos Gutiérrez-Ortega ◽  
Pablo Arias-Sanz ◽  
Javier Castro-Urda
Keyword(s):  
Connective Tissue ◽  
Inflammatory Reaction ◽  
Direct Contact ◽  
Wistar Rats ◽  
Tissue Response ◽  
New Mineral ◽  
Exact Test ◽  
Dorsal Area ◽  
Subcutaneous Connective Tissue ◽  
Hematoxylin Eosin

The aim of this paper was to assess the differences in tissue response to implantation during 15, 30 and 45 days in the subcutaneous connective tissue of Wistar rats from three biomaterials: Angelus MTA®, Theracal LC®, and Angelus MTA® to which 25% bioglass G3 was added. Twenty-four Wistar rats were used, the materials were inserted into the rat’s dorsal area in silicone tubes 5 mm long by 1.5 mm diameter. Histological reaction was assessed at 15, 30, and 45 days after implantation. They were then stained with hematoxylin eosin and evaluated by two observers. Data were analyzed using Fisher’s exact test and Mann–Whitney’s U test was used to determine the association between variables. Angelus MTA induced the formation of dystrophic calcifications twice as much as Theracal LC (p < 0.05). The addition of G3 did not affect the greater or lesser occurrence of calcifications (p > 0.05). Theracal LC and MTA plus G3 caused an inflammatory reaction, which was chronic at 15 days and decreased in intensity, almost disappearing after 45 days. Theracal LC, as well as Angelus MTA plus G3, were well tolerated when implanted in the subcutaneous connective tissue of rat. Histologically, no inconvenience was found for the use by direct contact of Theracal LC, and the mixture of MTA with 25% bioactive glass G3, in the tissue of Wistar rats.

scholarly journals Biocompatibility of a Calcium Hydroxide-Propolis Experimental Paste in Rat Subcutaneous Tissue

2014 ◽  
Vol 25 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Graziela Garrido Mori ◽  
Sindineia da Silva Rodrigues ◽  
Sheila Tieko Shibayama ◽  
Marcelo Pomini ◽  
Cristhiane Olivia Ferreira do Amaral
Keyword(s):  
Zinc Oxide ◽  
Connective Tissue ◽  
Calcium Hydroxide ◽  
Inflammatory Process ◽  
Subcutaneous Tissue ◽  
Histological Sections ◽  
Zinc Oxide Eugenol ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Hematoxylin And Eosin

Intracanal medications are fundamental for disinfection of the root canal system and participate in periapical repair, so their biocompatibility is of utmost importance to avoid tissue damage. This study evaluated the biocompatibility of a experimental paste of calcium hydroxide and propolis in the subcutaneous tissue of rats. The study was conducted on 15 male Wistar rats. Two incisions were made on the dorsal region of each animal for introduction of 4 tubes: one tube was empty; one contained zinc oxide-eugenol cement, and the two other tubes were filled with experimental paste. After 7, 14 and 30 days, the animals were euthanized and the specimens were subjected to histotechnical preparation. The hematoxylin and eosin-stained histological sections were analyzed by light microscopy. Scores were established according to the inflammatory process and statistically compared by the Tukey test (α=5%). The analysis of histological sections showed non-significant or mild inflammatory reaction in the connective tissue in contact with the empty tubes in all study periods while the contact of subcutaneous tissue with zinc oxide-eugenol elicited moderate or severe inflammation similarly without significant difference among the study periods. The connective tissue was moderately inflamed at 7 days when contacting the experimental paste, but the inflammatory process was non-significant or mild at 14 and 30 days. The experimental paste was biocompatible with the tissues after 14 days of subcutaneous implantation.

scholarly journals In vitro evaluation of apical microleakage using different root-end filling materials

2006 ◽  
Vol 14 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Márcia Carneiro Valera ◽  
Carlos Henrique Ribeiro Camargo ◽  
Alessandra Sverberi Carvalho ◽  
Eduardo Ramalho Pereira Gama
Keyword(s):  
Zinc Oxide ◽  
Root Apex ◽  
Tissue Fluid ◽  
Filling Material ◽  
Human Teeth ◽  
Root Canals ◽  
Filling Materials ◽  
Gutta Percha ◽  
Significant Difference

The objective of this study was to evaluate the apical leakage of retrograde cavities filled with Portland Cement (Concrebrás S/A-MG-Brazil), ProRoot MTA TM (Dentsply International, Johnson City, TN, USA) and Sealapex (Kerr Corporation, Orange, California, USA) with addition of zinc oxide (Odahcam Herpo Produtos Dentários Ltda, Rio de Janeiro, RJ, Brazil). Forty-two extracted single-rooted human teeth were decoronated and used for this study. The root canals were instrumented at 1.0mm short of the apical foramen using the step-back technique to an apical ISO size 60. The roots were obturated with gutta-percha points and sealer Sealapex (Kerr Corporation-USA) and then 3mm of each root apex was sectioned at a 90° angle. Ultrasonic retrograde preparation was performed with a diamond tip to 3mm depth and the roots were randomly divided into 3 groups according to the filling material: G1-Portland, G2-ProRoot MTA, G3- Sealapex zinc oxide-added cement. The root surfaces were covered with nail varnish up to 2mm from the apical foramen, immersed in simulated tissue fluid for 30 days, and then immersed in 0.2% Rhodamine B solution for 24 hours for evaluation of marginal leakage. The results showed mean leakage of 0.75, 0.35 and 0.35 for groups 1, 2 and 3, respectively; however, Kruskal-Wallis test revealed that there was no statistically significant difference among the results (p>0.05).

scholarly journals Tissue toxicity of root canal sealers

2004 ◽  
Vol 51 (3) ◽  
pp. 136-141 ◽  
Author(s):  
Mirjana Vujaskovic ◽  
Dragoljub Bacetic
Keyword(s):  
Connective Tissue ◽  
Root Canal ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Control Material ◽  
Tissue Sections ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions ◽  
Root Canal Sealers ◽  
Observation Period

The aim of this study was to evaluate tissue response to root canal sealers Tubuliseal and Sealapex. The sealers were freshly mixed and injected in the dorsal subcutaneous connective tissue of 12 Wistar rats.The observation periods were 7 days, 21days and 60 days. Four operative areas were formed ( 2 for test sealers, Tubliseal or Sealapex and 2 for control material) on each animal. Tissue sections were taken from selected sites. Each section included skin, subcutaneous connective tissue and underlying muscle tissue.All blocks were processed with the use of standardized histological procedures.The tissue reactions were studied under light microscopy. Different grades of tissue reaction to the tested materials were recorded as mild, moderate or severe inflammation. After seven days both root canal sealers showed severe inflammatory reaction of connective tissue in experimental animals.Tubuliseal caused prolonged moderate and mild inflammation. Sealapex caused mild inflammation which diminished at the end of the observation period. The results of this study demonstrated that Sealapex was better tolerated by tissue than Tubliseal.

scholarly journals Comparison of Rat Connective Tissue Response to BioMTA, Angelus MTA, and Root MTA

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Marzieh Alimohammadi ◽  
Sina Mirzaee-Rad ◽  
Farideh Feizi ◽  
Hadi Shirzad Juybari ◽  
Akam Saeidi ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Wistar Rats ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Control Group ◽  
Capsule Formation ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Difference ◽  
Fibrous Capsule Formation

Due to the widespread use of MTA in dentistry and various brands of this product, we decided to compare the three brands available in the country market by their biocompatibility. We divided 20 male Wistar rats into four groups. After local anesthesia and washing, we made two incisions on both sides (4 incisions in total). The experimental groups were Angelus MTA (Angelus, Brazil), BioMTA (CERKAMED, Poland), Root MTA (Dr. Lotfi, Tabriz, Iran), and the control group. The resulting paste was placed in a tube and implanted subcutaneously into male Wistar rats. Wistar rats were sacrificed 7, 15, 30, and 60 days later, with high anesthetic doses. The sample implanted in 10% formalin was stabilized after tissue processing and H&E staining under a microscope. The inflammatory reaction in the tissues received different scores at the beginning of the tube opening. BioMTA had the highest inflammatory response among the groups, but the difference was not statistically significant ( p  > 0.05). Also, there was no significant difference between the groups’ granulation and calcification ( p  < 0.05). There was a significant difference between BioMTA, Angelus MTA, Root MTA, and control groups in fibrous capsule formation ( p  < 0.05). Angelus MTA showed the lowest mean fibrous capsule formation in all periods. The effects of Angelus MTA, Root MTA, and BioMTA on connective tissue were investigated and compared. According to this study, these materials have good biocompatibility. According to the findings and statistical analysis, Angelus MTA has the most biocompatibility.

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