scholarly journals Adenosquamous carcinoma of the cervical oesophagus in a dog

2020 ◽  
Vol 50 (7) ◽  
Author(s):  
Francisca Maria Sousa Barbosa ◽  
Natanael Félix Silva Filho ◽  
Tolentino Lira Alcântra ◽  
Gisele Castro Menezes ◽  
Alex dos Santos ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Growth Pattern ◽  
Adenosquamous Carcinoma ◽  
Cellular Growth ◽  
Ventral Region ◽  
Immunohistochemical Examination ◽  
Neoplastic Cells ◽  
Cervical Oesophagus ◽  
First Report ◽  
Vacuolated Cytoplasm

ABSTRACT: A 9-year-old male Shih Tzu dog presented with a mass on the ventral region of the neck and developed ptyalism. Radiographs revealed a radiodense nodule located in the mid-third of the oesophagus compressing the trachea. Preoperative cytology showed large neoplastic cells with abundant vacuolated cytoplasm arranged in clusters. The nodule was removed by oesophagectomy and submitted for histopathology. Microscopically, the oesophageal mass was multilobulated and unencapsulated. It had a mixed cellular growth pattern with areas showing squamous and glandular differentiation. The squamous component of the tumor was formed by solid strands of neoplastic epithelial cells; many neoplastic cells had undergone central keratinization and sometimes formed keratin pearls. The deeper adenocarcinomatous portion of the tumor consisted of tubules filled with slightly basophilic mucinous material. Histological and immunohistochemical examination confirmed the diagnosis of primary adenosquamous carcinoma (ASC) of the esophagus. This is the first report of primary ASC of the cervical oesophagus in a dog.

Overexpression of aldolase A and cytokeratin 19 in ovine pulmonary adenocarcinoma

2012 ◽  
Vol 15 (4) ◽  
pp. 703-709 ◽  
Author(s):  
A. Kycko ◽  
M. Reichert
Keyword(s):  
Epithelial Cells ◽  
Pulmonary Adenocarcinoma ◽  
Cytokeratin 19 ◽  
Immunohistochemical Examination ◽  
Neoplastic Cells ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Immunohistochemical Studies ◽  
Aldolase A ◽  
Experimental Group ◽  
Dimensional Electrophoresis

Abstract Ovine pulmonary adenocarcinoma (OPA) is a transmissible lung cancer of sheep caused by jaagsiekte sheep retrovirus (JSRV). In the present study the protein profiles of five neoplastic and three non-neoplastic sheep lung tissues were examined for the identification of proteins overexpressed in ovine pulmonary adenocarcinoma. Lung sections of the experimental group of sheep were collected during necropsies for proteomic and immunohistochemical examination. Two dimensional electrophoresis (2DE) was performed using gel strips with immobilized pH gradient 3-10. As a result of 2DE gel analysis 14 spots characterized by over 2-fold higher expression in tumour proteomes were selected for mass spectrometry. In eleven spots more than one polypeptide was identified indicating overlapping of proteins in gels. In two spots demonstrating over 3-fold higher expression in OPA proteomes, single proteins: cytokerarin 19 (CK19) and aldolase A were identified. Immunohistochemical studies revealed that CK19 and aldolase A were expressed in the cytoplasm of epithelial cells of bronchioles in non-neoplastic lung sections, as well as epithelial cells of bronchioles and neoplastic cells in lung sections of OPA affected sheep. The results indicate that the overexpression of the two proteins reflects the presence of neoplastic cells in the lungs of OPA affected sheep.

Stromal cell and human prostatic epithelial cell in-vitro co-coltures: Growth and morphology

1996 ◽  
Vol 63 (1_suppl) ◽  
pp. 65-68
Author(s):  
S. De Angeli ◽  
A. Fandella ◽  
C. Gatto ◽  
S. Buoro ◽  
C. Favretti ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Marked Reduction ◽  
Stromal Cell ◽  
Optical Microscope ◽  
Neutral Red ◽  
Cellular Growth ◽  
Murine Fibroblasts ◽  
Prostatic Epithelial Cell

A study was carried out on the effect of stroma-epithelium interaction on cellular growth and morphology in co-coltures of U285 prostatic epithelial cells with human prostatic and esophageal stromal cells and with murine fibroblasts of the 3T3-J2 line. The proliferation rate was determined by growth tests of neutral red and kenacid blue. Morphological observations were made under optical microscope on the same cultures used for the growth tests. Results highlighted a marked reduction in cellular growth in the co-cultures compared to control cultures, as well as the tendency of the stromal and epithelial cells to re-organise themselves in pseudo-acinous structures.

Hepatic Myoepithelial Carcinoma in a Dog: Immunohistochemical Comparison With Other Canine Hepatic Carcinomas

2019 ◽  
Vol 56 (6) ◽  
pp. 889-894
Author(s):  
Yuka Tsuji ◽  
Mizuki Kuramochi ◽  
Takeshi Izawa ◽  
Hideo Akiyoshi ◽  
Jyoji Yamate ◽  
...  
Keyword(s):  
Smooth Muscle Actin ◽  
Myoepithelial Cells ◽  
Left Lobe ◽  
Myoepithelial Carcinoma ◽  
Hepatic Tumors ◽  
Muscle Actin ◽  
Hepatic Carcinoma ◽  
Neoplastic Cells ◽  
Immunohistochemical Studies ◽  
Vacuolated Cytoplasm

An 11-year-old female miniature Dachshund dog presented with a solid, soft, gray mass on the hepatic lateral left lobe. Histologically, the mass consisted of neoplastic proliferation of cells with round nuclei and eosinophilic and vacuolated cytoplasm arranged in alveolar, trabecular, and solid patterns. Immunohistochemically, the neoplastic cells were positive for pancytokeratin (CK AE1/AE3), CK5, CK14, vimentin, Sox9, and myoepithelial markers (α–smooth muscle actin, p63, and calponin). The morphological and immunohistochemical findings indicated a diagnosis of myoepithelial carcinoma. We conducted immunohistochemical studies on other representative canine hepatic tumors. Although the myoepithelial phenotype was not observed in the hepatocellular carcinoma, some tumor cells in cholangiocarcinoma showed immunohistochemical features of myoepithelium, suggesting that some neoplastic cells in cholangiocarcinoma may have the potential to differentiate into myoepithelial cells. To our knowledge, this is the first report in veterinary medicine of a hepatic carcinoma with a myoepithelial phenotype.

scholarly journals A Rare Case of Clear Cell Carcinoma, Müllerian Type in the Renal Pelvis of a 21-Year-Old Woman

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Diandra Perez ◽  
Rana Naous
Keyword(s):  
Cell Carcinoma ◽  
Renal Pelvis ◽  
Clear Cell ◽  
Clear Cell Carcinoma ◽  
Female Genital Tract ◽  
Neoplastic Cells ◽  
Primary Location ◽  
Foamy Macrophages ◽  
Vacuolated Cytoplasm

Clear Cell Carcinomas of Müllerian origin are extremely rare within the upper urinary system. Their morphology is identical to that of the Clear Cell Carcinomas of the female genital tract. When they arise in the urinary tract, it is thought to be due to ectopic Müllerian embryogenesis. Here, we present a case of a 21-year-old woman with a Clear Cell Carcinoma, Müllerian type, arising from the renal pelvis. Histologically, it consisted of tubulopapillary architecture with associated foamy macrophages and a mucinous background. The neoplastic cells exhibited variably sized round nuclei with prominent nucleoli, eosinophilic to vacuolated cytoplasm with occasional intracytoplasmic mucin vacuoles, and a hobnail appearance. Immunohistochemical stains showed that the neoplastic cells were positive for Pax-8, p53, CK7, HMWK 903, and INI-1 and focally positive for p504s (AMACR). The neoplastic cells were negative for GATA-3, CK5/CK6, p63, CK20, and CDX-2 immunostains, ruling out urothelial or enteric phenotype. Additional immunostains performed by an outside institution showed that the neoplastic cells were positive for HNF-1β. The overall morphology and immunophenotype were consistent with Clear Cell Carcinoma of Müllerian origin arising from the renal pelvis. Follow-up revealed no metastasis or other tumor sites, supporting that this was the primary location.

scholarly journals Pulmonary adenocarcinoma in cattle

2014 ◽  
pp. 4358-4363 ◽  
Author(s):  
Diogo Sousa Z ◽  
Luis Rivera C ◽  
Didier Quevedo C ◽  
Ana Claudia Gorino ◽  
Simone Biagio C ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tumor Cells ◽  
Adult Female ◽  
Histopathological Examination ◽  
Abdominal Cavity ◽  
Pulmonary Adenocarcinoma ◽  
Immunohistochemical Examination ◽  
Histopathological Findings ◽  
Hematoxylin Eosin ◽  
Neoplastic Epithelial Cells

ABSTRACTThe Macroscopic, histological and immunohistochemical aspects of lung acinar adenocarcinoma and the presence of nodules in the abdominal cavity of an adult female bovine are reported. In the necropsy analysis samples were collected from the: lung, heart, spleen, liver, pancreas, kidney, uterus, intestine, brain, and from nodules found in the lung and abdominal cavity, which were routinely processed to be stained by hematoxylin-eosin and for an immunohistochemistry exam with the antibodies: cytokeratin (dilution 1:200 μL) and vimentin (dilution 1:1000 μL). The histopathological examination revealed neoplastic epithelial cells with acini formation. The immunohistochemical examination of the tumor cells showed positive marking for cytokeratin and the absence of marking for vimentin. According to anatomical, morphological, and histopathological findings, as well as the result of the immunohistochemical examination, the tumor was characterized as lung acinar adenocarcinoma.

scholarly journals Neurenteric Cysts Found on the Outer Membrane of a Subdural Haematoma

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Satoshi Takahashi ◽  
Yoshio Tanizaki ◽  
Kazunori Akaji ◽  
Tadashige Kano ◽  
Ban Mihara ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Outer Membrane ◽  
Subdural Haematoma ◽  
Chronic Subdural Haematoma ◽  
Burr Hole ◽  
Pathologic Examination ◽  
First Report ◽  
Underlying Causes ◽  
Ciliated Epithelial Cells

We report on a patient initially diagnosed with a chronic subdural haematoma that was resistant to treatment. After the second burr hole craniostomy within a half month failed to resolve the subdural haematoma (SDH), we performed a craniotomy to identify the point of bleeding. Macroscopic evaluation showed that most of the outer membrane of the SDH was transparent; however, further examination revealed the presence of multiple white regions. Pathologic examination showed that the white regions were fluid filled and surrounded by columnar ciliated epithelial cells. These lesions were pathologically diagnosed as neurenteric cysts. To our knowledge, this is the first report on a patient with neurenteric cysts found on the outer membrane of a CSDH. We agree that a craniotomy is a treatment of last resort for recurrent CSDHs; however, sometimes this procedure can be very useful for identifying underlying causes of obstinate SDHs as well as for their treatment.

A case of hepatic carcinoid in a red deer hind (Cervus elaphus)

2011 ◽  
Vol 59 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Diana Žele ◽  
Mitja Gombač ◽  
Tanja Švara ◽  
Gorazd Vengušt
Keyword(s):  
Positive Reaction ◽  
Cervus Elaphus ◽  
Chromogranin A ◽  
Red Deer ◽  
Carcinoid Tumour ◽  
Neuron Specific Enolase ◽  
Neoplastic Cells ◽  
First Report ◽  
Strong Positive Reaction ◽  
Hepatic Carcinoid

A carcinoid tumour in the liver of a red deer hind (Cervus elaphus) is described. Macroscopically, the liver was considerably enlarged with multifocal, firm, yellow and red nodular neoplastic masses, which were histopathologically diagnosed as hepatic carcinoids. The diagnosis was confirmed by modified Grimelius staining, which demonstrated numerous small argyrophilic granules in the cytoplasm of neoplastic cells, and by immunohistochemistry. The neoplastic cells gave a strong positive reaction for neuron-specific enolase (NSE) and synaptophysin and a weak positive reaction for chromogranin A. To our knowledge, this is the first report of a hepatic carcinoid in red deer.

scholarly journals Increased sialylation of surface glycopeptides of human trophoblast compared with fetal cells from the same conceptus.

1978 ◽  
Vol 148 (4) ◽  
pp. 1087-1092 ◽  
Author(s):  
A Whyte ◽  
Y W Loke
Keyword(s):  
Sialic Acid ◽  
Cellular Growth ◽  
Neoplastic Cells ◽  
Double Labeling ◽  
Human Trophoblast ◽  
Cellular Behavior ◽  
Fetal Cells ◽  
Trophoblastic Cells ◽  
Metabolic Products

The surface glycopeptides of human trophoblastic cells have been compared with those of fetal cells from the same embryos using double-labeling methods with isotopes of L-fucose and D-glucosamine. A faster eluting, neuraminidase-sensitive, fraction was observed on Sephadex chromatography of the trophoblast spectra when D-glucosamine was used as precursor. Labeling with fucose did not appear to result in any differences, thus suggesting that the glycopeptides characertistic of trophoblast contained glucosamine-derived metabolic products, including sialic acid, but excluding fucose. This increased sialylation is similar to, but not identical with, modifications observed in neoplastic cells, and on this basis it is postulated that two species of glycopeptides may be involved in atypical cellular behavior. The first contains sialic acid and other sugars excluding fucose, and is associated with localized cellular growth and invasion. The second contains both sialic acid and fucose and is characteristic of neoplastic cells.

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