scholarly journals Effect of anti-inflammatory agents on the integration of autogenous bone graft and bovine bone devitalized matrix in rats

2008 ◽  
Vol 23 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Roberto Antoniolli da Silva ◽  
Djalma José Fagundes ◽  
Andréia Conceição Milan Brochado Antoniolli Silva ◽  
Karin Ellen Sisti ◽  
Themis Maria Milan Brochado de Carvalho ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Saline Solution ◽  
Diclofenac Sodium ◽  
Bovine Bone ◽  
Collagen Formation ◽  
Group I ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Group Ii

PURPOSE: To study the repair of bone defect filled with autograft or bovine bone devitalized matrix in rats under anti-inflammatory action. METHODS: Two hundred and forty Wistar rats were distributed to two groups of 120 animals each. A 2mm-diameter defect was created in the femoral diaphysis. Animals of Group I had the bone defect filled with autograft and those of Group II, with bovine bone devitalized matrix. Animals of each group were redistributed to four subgroups according to the intramuscular administration of anti-inflammatory drug or saline solution: A - diclofenac sodium; B - dexamethasone; C - meloxicam; D - saline solution. Evaluation periods were 7, 14 and 30 days. Histological evaluation consisted of quantifying the inflammatory process, the bone neoformation, the collagen formation and the presence of macrophages. RESULTS: Animals of Group I did not show significant difference considering inflammatory reaction. Significant and progressive increase of bone neoformation was observed in both groups. The animals that received meloxicam and autograft showed less collagen formation at 14 and 30 days. The number of macrophages was higher in Group II than in Group I. The animals treated with dexamethasone and saline solution did not show statistically significant difference. CONCLUSIONS: Diclofenac sodium and meloxicam delayed bone graft repair and dexamethasone did not interfere in it.

scholarly journals Comparison of Ellagic Acid and NSAI Agents in the Treatment of Achilles Tendon Lacerations: An Experimental Study in Rabbits

2019 ◽  
Vol 104 (11-12) ◽  
pp. 575-581
Author(s):  
Ertuğrul Allahverdi ◽  
Tülay Diken Allahverdi ◽  
Sevil Vural
Keyword(s):  
Achilles Tendon ◽  
Ellagic Acid ◽  
Diclofenac Sodium ◽  
Tendon Healing ◽  
Control Group ◽  
Group I ◽  
Significant Difference ◽  
Tendon Ruptures ◽  
Anti Inflammatory ◽  
Group Ii

Objective Although Achilles tendon ruptures can have many causes, they are known to develop most commonly with trauma. Nonsteroidal anti-inflammatory drugs (NSAID) and low doses of corticosteroids are used in the medical treatment of tendon ruptures. Ellagic acid (EA), which also has an anti-inflammatory effect, has been reported to show its effect via cyclooxygenase 2 (COX2) inhibition as well. The effects of EA and diclofenac sodium on tendon healing were compared in this study. Methods We used a total of 18 male New Zealand rabbits in 3 groups with 6 in each. The study was performed under general anesthesia with a xylazine-ketamine combination. After a defect was created in the right Achilles tendon of all the rabbits, group I was administered diclofenac sodium and group II was administered EA for 1 week, whereas the control group (group III) was not administered anything. Postoperative follow-up was provided for all groups. Results Euthanasia was performed in all subjects at the end of the eighth week, and the tendons were compared in terms of macroscopic and histopathologic features and tensile resistance. Although there was no statistically significant difference in the tensile resistance Newton values of group I and group II, these values were higher than in the control group, and the NSAI group values were statistically significantly higher than in the control group. Conclusions We concluded that EA and NSAIs could be effective in the recovery of tendon integrity and tensile strength and increasing the movement capacity in pathology caused by tendon damage because of their anti-inflammatory features.

scholarly journals Synergistic Effects of Growth Factor, Bone Graft, and Resorbable Barrier Membrane in Management of Dehiscence and Fenestration of Dental Implants

2017 ◽  
Vol 8 (3) ◽  
pp. 177-182
Author(s):  
Umesh P Verma
Keyword(s):  
Growth Factor ◽  
Bone Graft ◽  
Dental Implants ◽  
Synergistic Effects ◽  
Test Group ◽  
Control Group ◽  
Barrier Membrane ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

ABSTRACT Aim The present study was primarily designed to evaluate the outcome of guided bone graft regeneration in peri-implant defects by combining recombinant human platelet-derived growth factor-BB (rhPDGF-BB) and granules of beta-tricalcium phosphate (B-TCP) mounted by resorbable biomesh membrane. Secondary objective was to determine the value of resorbable barrier membrane to improve the efficacy of the growth factormediated regeneration. Materials and methods A randomized controlled study comprised 14 participants (8 males and 6 females, mean age 37 years, range 19—55 years), in which a total of 15 implants (10 in maxilla and 5 in the mandible) were placed. Fifteen implant sites were randomly divided by picking a code into three groups: Test group I (n = 5) ß-TCP + rhPDGF (0.3 mg/mL) + biomesh, test group II (n = 5) ß-TCP + rhPDGF, and control (n = 5) ß-TCP + biomesh. The experimental site was examined clinically for the gingival status and radiographically for the bone status. Results Statistically significant difference in preoperative and postoperative measurements was observed for test groups I and II in all the parameters except width; in contrast, there was no significant difference observed for the control group from baseline to 5 months postoperatively. On intergroup comparison, statistically significant difference was observed between test group I vs control group and test group II vs control group, but it was not significant between test groups I and II, which was further confirmed using global performance scale score. Conclusion It concluded that rhPDGF-BB and ß-TCP mounted by resorbable biomesh membrane played a synergistic role in the management of peri-implant defects. Clinical significance Bone regenerated using ß-TCP with rhPDGF-BB in the reversal of peri-implant defects. How to cite this article Arora R, Verma UP, Dixit J, Lal N. Synergistic Effects of Growth Factor, Bone Graft, and Resorbable Barrier Membrane in Management of Dehiscence and Fenestration of Dental Implants. World J Dent 2017;8(3):177-182.

scholarly journals The effect of implantation materials based on equine and bovine xenogenic bone extracellular matrix on the formation of extracellular neutrophilic traps (experimental study)

2020 ◽  
Vol 26 (4) ◽  
pp. 571-575
Author(s):  
O.V. Diuriagina ◽  
◽  
M.V. Chepeleva ◽  
E.I. Kuznetsova ◽  
M.A. Kovinka ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Bone Defect ◽  
Experimental Period ◽  
Cylindrical Shape ◽  
Nuclear Transformation ◽  
Bovine Bone ◽  
Group I ◽  
Group Ii ◽  
Equine Bone

Purpose To study the effect of osteoplastic materials based on the extracellular xenomatrix of bovine and equine bone tissue on the formation of neutrophil extracellular traps (NETs) in the peripheral blood of rabbits in the early post-operative period after implantation. Materials and methods The study was carried out on 18 male rabbits of the Soviet Chinchilla breed, aged from 8 months to 1.2 years, weighing from 3.0 to 4.5 kg. A perforated bone defect of a cylindrical shape measuring 2 x 6 mm in the distal metaphysis of the right and left femurs was modeled in the animals. The rabbits were divided into three groups, six animals each. In group I, the bone defect was left unfilled; in group II, the defect was filled with a bovine bone tissue xenomatrix, and an equine bone tissue xenomatrix was implanted in group III animals. The implantation material had the appearance of a yellowish crumb with a particle size of 0.5– 1 mm. Blood smears stained according to Romanovsky-Giemsa were used for counting extracellular neutrophil traps (NETs). The percentage of neutrophils that passed the stages of nuclear transformation and emitted free chromatin into the extracellular space in the form of network-like structures was calculated. Results On days 3–7 of the experiment, the number of NETs increased in the early stages of NETosis in all groups. There were no significant differences between the groups. In group I, on days 7 and 14, the number of early forms of NETs (stages 1a and 1b) returned to the values of the preoperative period. In groups II and III, normalization of NETs (stage 1a) did not occur, and the content of NETs (stage 1b) returned to the initial level only by day 30 of the experiment. On days 3, 7, 14, the number of mature NETs increased in all groups. The highest values were noted in group II, where the bovine xenogeneic matrix was implanted. Conclusion Implantation materials based on the extracellular matrix of equine and bovine xenogeneic bone stimulate excessive formation of early NETs on days 14–30 of the experimental period in response to xenotransplantation. Xenomaterials of bovine bone tissue, in comparison with xenomaterials of equine bone tissue, induce a more pronounced inflammatory reaction in the nearest time after defect filling, which is manifested by higher production of mature NETs on days 3–14 of the experiment.

scholarly journals Effect of small dose intermittent IV pethidine in combination with diclofenac for post operative pain relief

2014 ◽  
Vol 22 (2) ◽  
pp. 40-47
Author(s):  
Debabrata Banik ◽  
Md Abdul Hye ◽  
AKM Akhtaruzzaman ◽  
Lutful Aziz ◽  
Sabina Yeasmeen ◽  
...  
Keyword(s):  
Abdominal Surgery ◽  
Diclofenac Sodium ◽  
Perioperative Period ◽  
Convincing Evidence ◽  
Acute Type ◽  
Post Operative Period ◽  
Group I ◽  
Post Operative Pain ◽  
Significant Difference ◽  
Group Ii

Background: Post operative pain is an acute type of pain. There is convincing evidence that unrelieved post operative pain may result in harmful physiological and psychological effects with significant morbidity and even mortality. Complete and effective pain management is an essential for the patient who recovers from surgery. For this reason we used easy available drugs opioid, NSAID and its combination for better post operative analgesia with the aim of avoiding expensive patient controlled analgesia technique, infusion or intramuscular painful roout of administration. Methods: 120 patients ASA grade-I and II were selected for post operative pain releive in upper and lower abdominal surgery. All patients divided into three groups and each group are equal in number and same type of surgery. Among the three groups, two groups received small intermittent intravenous pethidine and diclofenac pre and post operatively and other groups received intra muscular pethidine with diclofenac pre and post operatively (controlled group). All vital parameter and pain score both VAS and VRS were recorded in perioperative period up to 24 hours postoperatively. Result: Good level of anaesthesia was achieved in all groups (VAS>30mm) of upper and lower abdominal surgery except early post operative period. In early post operative period significant difference of VAS in group II and III (P<.01) was found with group I. But after half an hour and one hour significant difference of VAS in group II and III (P<0.02, P<0.001) was found with group I. Over all excellent analgesia was possible in group II VAS, VRS always more than 30mm and all vital sign were stable. Conclusion: Small intravenous intermittent dose of pethidine in combination with diclofenac sodium effectively controlled post-operative pain. DOI: http://dx.doi.org/10.3329/jbsa.v22i2.18140 Journal of BSA, 2009; 22(2): 40-47

scholarly journals A Clinical Evaluation of Anorganic Bovine Bone Graft Plus 10% Collagen with or without a Barrier in the Treatment of Class II Furcation Defects

2006 ◽  
Vol 7 (1) ◽  
pp. 60-70 ◽  
Author(s):  
Krishnanjaneya Pathakota Reddy ◽  
Dilip Gopinath Nayak ◽  
Ashita Sadananda Uppoor A
Keyword(s):  
Bone Graft ◽  
Gingival Recession ◽  
Class Ii ◽  
Bovine Bone ◽  
Hard Tissue ◽  
Barrier Membranes ◽  
Group I ◽  
Probing Depth ◽  
Furcation Defects ◽  
Group Ii

Abstract The use of bone replacement grafts with barrier membranes in class II furcation defects are aimed at improving the outcome of the regenerative technique. In this regard, however, there is a paucity of studies comparing the results obtained with bone grafts alone or in combination with barrier membranes. The aim of this study was to clinically compare an anorganic bovine bone graft plus 10% collagen (BO) with or without a bioresorbable collagen barrier (BG) in human mandibular molar class II furcation defects. Methods and Materials Twenty mandibular class II furcation defects (ten patients with bilateral defects) were treated either with BO (group I) or a combination of BO/BG (group II). Each defect was randomly assigned to either group I or group II. The soft tissue and hard tissue measurements including vertical probing depth (VPD), horizontal probing depth (HPD), clinical attachment level (CAL), gingival recession (GR), vertical depth of furcation defect (VDF), and horizontal depth of furcation defect (HDF) were recorded at baseline and six months after surgery. Results Both treatment procedures resulted in statistically significant reduction in VPD and HPD, gain in CAL, and reduction in VDF and HDF. There was a statistically significant difference between group I and group II in all soft and hard tissue parameters with the exception of VPD reduction and gingival recession. Conclusion The findings of this study suggest superior clinical results with BO/BG treatment when compared to BO treatment in mandibular class II furcation defects. Citation Reddy KP, Nayak DG, Uppoor A AS. A Clinical Evaluation of Anorganic Bovine Bone Graft Plus 10% Collagen with or without a Barrier in the Treatment of Class II Furcation Defects. J Contemp Dent Pract 2006 February;(7)1:060-070.

scholarly journals The Comparison of the Effects of Ellagic Acid and Diclofenac Sodium on Intra-Abdominal Adhesion: An In Vivo Study in the Rat Model

2014 ◽  
Vol 99 (5) ◽  
pp. 543-550 ◽  
Author(s):  
Tulay Diken Allahverdi ◽  
Ertuğrul Allahverdi ◽  
Sadık Yayla ◽  
Turgay Deprem ◽  
Oğuz Merhan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ellagic Acid ◽  
Diclofenac Sodium ◽  
Peritoneal Adhesion ◽  
Peritoneal Adhesions ◽  
Group Iii ◽  
Group I ◽  
Anti Inflammatory ◽  
Group Ii

Abstract Peritoneal adhesions are seen frequently after abdominal surgery and can cause serious complications. We aimed to evaluate the effects of the oral use of diclofenac sodium and ellagic acid on formation of postoperative adhesions in rats Studies have shown that agents with anti-inflammatory properties and antioxidant substances can prevent adhesion by decreasing oxidative stress. We compared and evaluated the effects of ellagic acid that has strong antioxidant and anti-inflammatory properties and the nonsteroidal anti-inflammatory diclofenac sodium on peritoneal adhesion development in our experimental study. Laparotomy was performed with a midline incision under general anesthesia and an adhesion model was created on the antimesenteric side of the cecum in Groups I, II, and III. Group I received 85 mg/kg ellagic acid and Group II, 50 mg/kg diclofenac sodium through the nasogastric catheter while Group III received no medication. Only laparotomy was performed in Group IV. The rats were sacrificed at the end of the 14th day. Following macroscopic scoring, tissue samples were removed and subjected to biochemical and histopathologic evaluation. The degree of adhesion and the malondialdehyde level were decreased (P &lt; 0.05), and glutathione level increased (P &lt; 0.05) in Group I compared to Group II and Group III. The effects of ellagic acid on the prevention of peritoneal adhesion were found to be stronger than diclofenac sodium. This can be explained by the fact that ellagic acid is a strong antioxidant and decreases oxidative stress with anti-inflammatory and anti-angiogenic effects.

Comparative Assessment of Autogenous Cancellous Bone Graft and Bovine-Derived Demineralized Bone Matrix for Secondary Alveolar Bone Grafting in Patients With Unilateral Cleft Lip and Palate

2021 ◽  
pp. 105566562110251
Author(s):  
Vijay Kumar ◽  
Vidya Rattan ◽  
Sachin Rai ◽  
Satinder Pal Singh ◽  
Jai Kumar Mahajan
Keyword(s):  
Bone Graft ◽  
Iliac Crest ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Volumetric Analysis ◽  
Group I ◽  
Alveolar Bone Grafting ◽  
Group Ii ◽  
Demineralized Bone

Objective: Comparison between bovine-derived demineralized bone matrix (DMBM) and iliac crest graft over long term for secondary alveolar bone grafting (SABG) in patients with unilateral cleft lip and palate (UCLP) in terms of radiological and clinical outcomes. Design: Prospective, randomized, parallel groups, double-blind, controlled trial. Setting: Unit of Oral and Maxillofacial Surgery, Oral Health Science Centre, Postgraduate Institute of Medical Education & Research, Chandigarh. Participants: Twenty patients with UCLP. Interventions: Patients were allocated into group I (Iliac crest bone graft) and group II (DMBM) for SABG. Outcomes were assessed at 2 weeks, 6 months, and then after mean follow-up period of 63 months. Outcomes Measures: Volumetric analysis of the grafted bone in the alveolar cleft site was done through cone beam computed tomography using Cavalieri principle and modified assessment tool. Clinical assessment was performed in terms of pain, swelling, duration of hospital stay, cost of surgery, alar base symmetry, and donor site morbidity associated with iliac crest harvesting. Results: Volumetric analysis through Cavalieri principle revealed comparable bone uptake at follow-up of 6 months between group I (70%) and group II (69%). Modified assessment tool showed no significant difference between horizontal and vertical bone scores over short- and long-term follow-up. In group II, there was higher cost of surgery, but no donor site morbidity unlike group I. Conclusions: Demineralized bone matrix proved analogous to iliac crest bone graft as per volumetric analysis over shorter period. However, although statistically insignificant, net bone volume achieved was lower than the iliac crest graft at longer follow-up.

scholarly journals Impact of hemodialysis on the wellbeing of chronic kidney diseases patients: a pre-post analysis

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Um-e-Kalsoom ◽  
Sabiha Khan ◽  
Israr Ahmad
Keyword(s):  
Quality Of Life ◽  
Social Support ◽  
Perceived Social Support ◽  
Kidney Diseases ◽  
Chronic Kidney Diseases ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
The Impact

Abstract Background Hemodialysis may have serious psychological impact upon patients suffering from chronic kidney diseases. The aim of the present study is to investigate the impact of hemodialysis on the wellbeing of individuals with chronic kidney diseases (CKD). Result A sample consists of (N = 100) CKD patients referred from neurology ward of Leady Reading Hospital Peshawar. Data was collected from both male (50%) and female (50%) in 2017. Participants were divided into two groups on the basis of pre-set criteria. In group I, individuals with 4–5 stage of CKD referred first time for dialysis treatment were recruited. Group II comprised of CKD patients with 1–3 stage. Demographic data sheet, Pakistan Anxiety and Depression, WHO Quality of Life scale, and Perceived Social support scale (PSS) were used to test the hypotheses. Paired sample t test was use to see the difference between pre- and post-analysis of depression, anxiety, QOL, and PSS in group I (experimental group). Results suggests significant difference on depression (p > .001), anxiety (p > .001), and QOL (p > .001), while no significant difference was reported on perceived social support (p <.673). Findings also indicate no significant difference between group I and group II on QOL depression, anxiety, and PSS. Conclusion The findings concluded that patients under hemodialysis treatment suffered from depression, anxiety, and poor quality of life.

Role of Cord Blood Carboxyhemoglobin in Detecting Significant Hyperbilirubinemia in Term Neonates with ABO Alloimmunization

2021 ◽  
Author(s):  
Mahir Tıraş ◽  
Emrah Can ◽  
Şahin Hamilçıkan
Keyword(s):  
Cord Blood ◽  
Total Serum ◽  
Healthy Controls ◽  
Term Neonates ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Positive Direct ◽  
Severe Hyperbilirubinemia

Objective This study aimed to assess whether cord blood carboxyhemoglobin (COHb) levels in jaundiced term neonates with and without a positive direct Coombs test (DCT) and in healthy controls could be used as a predictor of severe hyperbilirubinemia. The percentage of cord blood COHb should be higher among neonates with Coombs-positive ABO hemolytic disease than among those with Coombs-negative ABO incompatibility and higher than that of ABO-compatible control neonates. Study Design This cross-sectional descriptive study of 198 term neonates comprised three subgroups: group I featured 68 DCT-positive ABO-incompatible neonates (ABO + DCT), group II featured 60 DCT-negative ABO-incompatible neonates with hyperbilirubinemia (ABO–DCT), and group III featured 70 healthy controls. COHb was determined by an OSM3 hemoximeter. Results Group I differed from groups II and III for cord blood bilirubin, cord blood hemoglobin, and cord blood hematocrit. Groups I and II had higher mean total serum bilirubin (TSB) levels than group III, while there was no difference in the mean TSB levels between groups I and II. There was no significant difference between the COHb group means for groups I, II, and III (p = 0.98). The area under the receiver operating characteristic curve calculated for group I/group III and group II/group III were found to be 0.62 and 0.54, respectively. Conclusion COHb levels did not prove to be superior to the DCT for predicting the risk of developing severe hyperbilirubinemia in term neonates. Key Points

scholarly journals Regenerative Therapy Modality for Treatment of True Combined Endodontic-Periodontal Lesions: A Randomized Controlled Clinical Trial

2021 ◽  
Vol 18 (12) ◽  
pp. 6220
Author(s):  
Reham AlJasser ◽  
Sundus Bukhary ◽  
Mohammed AlSarhan ◽  
Dalal Alotaibi ◽  
Saleh AlOraini ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Grafting ◽  
Endodontic Treatment ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Bony Defect ◽  
Keratinized Tissue ◽  
Group I ◽  
Significant Difference ◽  
Grafting Procedure

The aim of this in-vivo study was to evaluate/compare the clinical periodontal parameters in patients with true combined endo-perio lesions (EPL), treated with gutta-percha (GP) and mineral trioxide (MTA) as an obturation material alone and with addition of bone grafting in such lesions. 120 Saudi patients (mean age = 41yrs) diagnosed with true combined EPL participated in this study. Group I (control group, n = 30) was treated with conventional endodontic treatment using GP for obturation. Group II (n = 30) was treated with conventional endodontic treatment using MTA for obturation. Group III (n = 30) was treated with conventional endodontic treatment using GP for obturation + grafting procedure to fill the bony defect. Group IV (n = 30) was treated with conventional endodontic treatment using MTA for obturation + grafting procedure to fill the bony defect. Clinical parameters (Pocket depth (PD); Clinical attachment loss (CAL); keratinized tissue width (KTW); gingival phenotype (G.Ph.) and Cone Beam Computed Tomography Periapical Index (CBCTPAI)) were recorded and compared at baseline, 3, 6, 12 months’ interval. For the groups III and IV, CBCTPAI showed significant difference (p < 0.0001) with the other groups at 6 months and 1-year interval. The group with MTA + bone graft showed 76% and 90% patients with 0 score at 6 months and 1-year follow-up, respectively. Comparison of mean values of PD among study groups at 3 months, 6 months and 1 year showed significant difference at 3 months, whereas the mean PD values of subjects in GP + bone graft showed significantly higher PD values than other 3 groups (p = 0.025). Use of GP and MTA for root canal obturation along with periodontal therapy and bone augmentation helps in resolving complex endo-perio lesions. Bone grafting in addition to obturation with MTA was found to be the best treatment strategy in management of EPL cases and is recommended for clinicians who are treating EPL patients.

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