scholarly journals On the accuracy of the probe-sample contact stiffness measured by an atomic force microscope

2019 ◽  
Vol 10 (6) ◽  
pp. 642-653 ◽  
Author(s):  
A.V. Ankudinov
Keyword(s):  
Atomic Force Microscope ◽  
Contact Stiffness ◽  
Atomic Force

Nonlinear vibration of rectangular atomic force microscope cantilevers by considering the Hertzian contact theory

2010 ◽  
Vol 88 (5) ◽  
pp. 333-348 ◽  
Author(s):  
Ali Sadeghi ◽  
Hassan Zohoor
Keyword(s):  
Atomic Force Microscope ◽  
Timoshenko Beam ◽  
Frequency Ratio ◽  
Contact Stiffness ◽  
Sample Surface ◽  
Hertzian Contact ◽  
Nonlinear Frequency ◽  
Lateral Contact ◽  
Atomic Force ◽  
Contact Position

The nonlinear flexural vibration for a rectangular atomic force microscope cantilever is investigated by using Timoshenko beam theory. In this paper, the normal and tangential tip–sample interaction forces are found from a Hertzian contact model and the effects of the contact position, normal and lateral contact stiffness, tip height, thickness of the beam, and the angle between the cantilever and the sample surface on the nonlinear frequency to linear frequency ratio are studied. The differential quadrature method is employed to solve the nonlinear differential equations of motion. The results show that softening behavior is seen for most cases and by increasing the normal contact stiffness, the frequency ratio increases for the first mode, but for the second mode, the situation is reversed. The nonlinear-frequency to linear-frequency ratio increases by increasing the Timoshenko beam parameter, but decreases by increasing the contact position for constant amplitude for the first and second modes. For the first mode, the frequency ratio decreases by increasing both of the lateral contact stiffness and the tip height, but increases by increasing the angle α between the cantilever and sample surface.

A fresh insight into the non-linear vibration of double-tapered atomic force microscope cantilevers by considering the Hertzian contact theory

2010 ◽  
Vol 225 (1) ◽  
pp. 233-247 ◽  
Author(s):  
A Sadeghi ◽  
H Zohoor
Keyword(s):  
Atomic Force Microscope ◽  
Timoshenko Beam ◽  
Frequency Ratio ◽  
Contact Stiffness ◽  
Sample Surface ◽  
Hertzian Contact ◽  
Linear Frequency ◽  
Atomic Force ◽  
Non Linear ◽  
Contact Position

The non-linear flexural vibration for a double-tapered atomic force microscope cantilever has been investigated by using the Timoshenko beam theory. In this article, the normal and tangential tip—sample interaction forces are found from the Hertzian contact model, and the effects of the contact position, normal and lateral contact stiffness, height of the tip, thickness of the beam, angle between the cantilever and the sample surface, and breadth and height taper ratios on the non-linear frequency to linear frequency ratio have been studied. The differential quadrature method is employed to solve the non-linear differential equations of motion. The results show that the softening behaviour is seen for all cases. The non-linear frequency to linear frequency ratio increases by increasing the Timoshenko beam parameter and breadth and height taper ratios, but decreases by increasing the contact position for the first and second modes. For the first vibrational mode, the non-linear frequency to linear frequency ratio increases by increasing the height of the tip and the angle α between the cantilever and sample surface. By increasing the normal contact stiffness, the frequency ratio increases for the first mode.

Increasing the Image Contrast of Atomic Force Microscope by Using Improved Rectangular Micro Cantilever

2011 ◽  
Vol 110-116 ◽  
pp. 4888-4892
Author(s):  
Ali Sadeghi
Keyword(s):  
Atomic Force Microscope ◽  
Contact Stiffness ◽  
Beam Theory ◽  
Flexural Vibration ◽  
Sample Surface ◽  
Limited Range ◽  
Image Contrast ◽  
Atomic Force ◽  
Afm Cantilever ◽  
Micro Cantilever

The resonant frequency of flexural vibrations for an atomic force microscope (AFM) cantilever has been investigated using the Euler-Bernoulli beam theory. The results show that for flexural vibration the frequency is sensitive to the contact position, the first frequency is sensitive only to the lower contact stiffness, but high order modes are sensitive in a larger range of contact stiffness. By increasing the height H, for a limited range of contact stiffness the sensitivity to the contact stiffness increases. This sensitivity controls the image contrast, or image quality. Furthermore, by increasing the angle between the cantilever and sample surface, the frequency decreases.

scholarly journals Измерения контактной жесткости в атомно-силовом микроскопе

2020 ◽  
Vol 90 (11) ◽  
pp. 1951
Author(s):  
А.В. Анкудинов ◽  
М.М. Халисов
Keyword(s):  
Atomic Force Microscope ◽  
Analytical Model ◽  
Contact Interaction ◽  
Relative Position ◽  
Mechanical Characteristics ◽  
Contact Stiffness ◽  
Sample Surface ◽  
Atomic Force ◽  
Imaging Mode ◽  
Cantilever Probe

A method is proposed for increasing the accuracy of nanomechanical measurements in an atomic force microscope. To describe the contact interaction of the cantilever with the sample, an analytical model was used that takes into account the following factors: the cantilever probe sticks to the sample surface or slides along it, the geometric and mechanical characteristics of the sample and cantilever, and their relative position. Under the assumption of sliding, a filter was developed to correct the signals of contact stiffness and deformation measured on a sample with a developed relief. The use of the filter is illustrated in images obtained in an atomic force microscope with an imaging mode based on point-by-point registration of the force quasistatic interaction of the cantilever probe with the sample.

Contact Stiffness Measurements with an Atomic Force Microscope

2020 ◽  
Vol 65 (11) ◽  
pp. 1866-1872
Author(s):  
A. V. Ankudinov ◽  
M. M. Khalisov
Keyword(s):  
Atomic Force Microscope ◽  
Contact Stiffness ◽  
Atomic Force

Characterization of photosystem II with the atomic-force microscope

1992 ◽  
Vol 50 (2) ◽  
pp. 1146-1147
Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon
Keyword(s):  
Photosystem Ii ◽  
Atomic Force Microscope ◽  
Three Dimensional ◽  
Biologically Active ◽  
Atomic Force ◽  
Freeze Etching ◽  
Image Averaging ◽  
Oxygen Evolving ◽  
Averaging Techniques

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.

Imaging polymers, proteins and dna in aqueous solutions with the atomic force microscope

1989 ◽  
Vol 47 ◽  
pp. 32-33
Author(s):  
S.A.C. Gould ◽  
B. Drake ◽  
C.B. Prater ◽  
A.L. Weisenhorn ◽  
S.M. Lindsay ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Aqueous Solutions ◽  
Atomic Force Microscope ◽  
Piezoelectric Crystal ◽  
Atomic Force ◽  
Structure Of Molecules ◽  
Optical Lever

The atomic force microscope (AFM) is an instrument that can be used to image many samples of interest in biology and medicine. Images of polymerized amino acids, polyalanine and polyphenylalanine demonstrate the potential of the AFM for revealing the structure of molecules. Images of the protein fibrinogen which agree with TEM images demonstrate that the AFM can provide topographical data on larger molecules. Finally, images of DNA suggest the AFM may soon provide an easier and faster technique for DNA sequencing.The AFM consists of a microfabricated SiO2 triangular shaped cantilever with a diamond tip affixed at the elbow to act as a probe. The sample is mounted on a electronically driven piezoelectric crystal. It is then placed in contact with the tip and scanned. The topography of the surface causes minute deflections in the 100 μm long cantilever which are detected using an optical lever.

The atomic-force microscope and its future in biology

1993 ◽  
Vol 51 ◽  
pp. 704-705
Author(s):  
Jean-Paul Revel
Keyword(s):  
Silicon Nitride ◽  
Laser Beam ◽  
Atomic Force Microscope ◽  
Scanning Tunneling Microscope ◽  
Point Of View ◽  
Scanning Tunneling ◽  
Close Relative ◽  
Tunneling Microscope ◽  
Atomic Force ◽  
Sharp Point

The last few years have been marked by a series of remarkable developments in microscopy. Perhaps the most amazing of these is the growth of microscopies which use devices where the place of the lens has been taken by probes, which record information about the sample and display it in a spatial from the point of view of the context. From the point of view of the biologist one of the most promising of these microscopies without lenses is the scanned force microscope, aka atomic force microscope.This instrument was invented by Binnig, Quate and Gerber and is a close relative of the scanning tunneling microscope. Today's AFMs consist of a cantilever which bears a sharp point at its end. Often this is a silicon nitride pyramid, but there are many variations, the object of which is to make the tip sharper. A laser beam is directed at the back of the cantilever and is reflected into a split, or quadrant photodiode.

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