scholarly journals Controlling Anthracnose Disease of Locally Chili in Marginal Wetland using Endophytic Indigenous Microbes and Kalakai (Stenochlaena palustris) Leaf Extract

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ismed Setya Budi ◽  
Mariana Mariana
Keyword(s):  
Leaf Extract ◽  
Microbial Consortium ◽  
In Vitro Test ◽  
Positive Role ◽  
Anthracnose Disease ◽  
Vitro Test

<p>The research aims were to get the indigenus endophytic microbial consortium and to test the potency of kalakai leaf extract as biopesticides and biofertilizer on chili plant specific to wetlands (i.e., var. Hiyung). The microbes capable of inhibiting the growth of anthracnose have been performed on in-vitro test in pairs method.  It was found that 12 isolates have the ability to inhibit the growth of pathogens.  However, based on the results of a confirmatory endophytic test only three isolates had positive role as endophytic in chili plants, namely <em>Trichoderma</em> sp DN3, <em>Trichoderma</em> sp AK2, and <em>Trichoderma</em> sp BT1. The results of the effectiveness of each treatment on chilli plants in the greenhouse and the field shows that the application of endophytic could inhibit the development of anthracnose and spur the growth of plants. It could be concluded that the applications of <em>kalakai</em> leaf extract at the rate of 30 mL/plant can function as biopesticides and biofertilizer.</p>

scholarly journals Potential of Chromolaena odorata Leaf as A Cure of Aeromonas hydrophila on Giant Gouramy (Osphronemus gouramy)

2007 ◽  
Vol 4 (2) ◽  
pp. 139 ◽  
Author(s):  
Y. Hadiroseyani ◽  
. Hafifuddin ◽  
M. Alifuddin ◽  
H. Supriyadi
Keyword(s):  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
In Vitro Study ◽  
In Vitro Test ◽  
Chromolaena Odorata ◽  
In Vivo Test ◽  
Treated Fish ◽  
Vitro Test

<p>This study was conducted to examine the potency of <em>Chromolaena odorata</em> leaf extract as a medicine for skin eruption disease caused by  Aeromonas hydrophila in giant gouramy <em>Osphronemus gouramy</em>.  Leaf extract of Chromolaena odorata for in vitro test was 0 (as control), 13000, 15000, 17000, 19000 and 21000 ppm, poured onto TSA medium containing bacteria 103 cfu/ml, and then is incubated for 24 hours. In vivo test was performed by injecting bacteria 0.1 ml of 10<sup>9</sup> cfu/ml intramuscularly into giant gouramy (14 g weight), and then  fish were maintained in the water containing 15000 ppm of Chromolaena odorata leaf extract. In vitro study showed that prevention area of leaf extract against Aeromonas hydrophila was increase by increasing the concentration of leaf extract used, reached 9,33 mm.  Prevention zone of leaf extract by difusion tends to constant, reached 7,6 mm. By in vivo test, survival rate of giant gouramy infected by <em>Aeromonas hydrophila</em> was no significantly different between dosages of leaf extract.  All treated fish, excluded control died after 24 hours infection.</p> <p>Keywords: <em>Aeromonas hydrophila</em>, <em>Osphronemus gouramy</em>, <em>Chromolaena odorata</em></p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui potensi daun kirinyuh <em>Chromolaena odorata</em> sebagai obat untuk penyakit cacar yang diakibatkan oleh <em>Aeromonas hydrophila</em> pada ikan gurame <em>Osphronemus gouramy</em>. Konsentrasi ekstrak daun <em>Chromolaena odorata</em> untuk uji <em>in vitro</em> adalah 13000, 15000, 17000, 19000 dan 21000 serta 0 ppm sebagai kontrol, yang diletakkan di atas media TSA yang telah mengandung biakan bakteri 103 cfu/ml dan diinkubasi selama 24 jam. Uji <em>in vivo</em> dilakukan dengan menginjeksikan bakteri  sebanyak 0,1 ml (10<sup>9</sup> cfu/ml) secara intramuskular ke ikan gurame (berat 14 g) dan kemudian ikan dipelihara dalam air yang mengandung ekstrak daun kirinyuh 15000 ppm. Hasil uji <em>in virto</em> menunjukkan bahwa semakin tinggi konsentrasi ekstrak daun kirinyuh basah semakin efektif dalam menghambat perkembangan <em>A. hydrophila</em> dengan zona hambat tertinggi mencapai 9,33 mm. Zona hambat yang dihasilkan melalui metode difusi cenderung konstan, mencapai 7,6 mm. Melalui uji <em>in vivo</em>, tingkat kelangsungan hidup ikan gurame yang tidak berbeda nyata pada masing-masing perlakuan, bahkan terjadi kematian total dalam 24 jam pada semua perlakuan, kecuali kontrol.</p> <p>Kata kunci: <em>Aeromonas hydrophila</em>, <em>Osphronemus gouramy</em>, <em>Chromolaena odorata</em></p>

scholarly journals Safety and Efficacy of Prosopis juliflora Leaf Extract as a Potential Treatment against Visceral Leishmaniasis in Balb/c Mice

2021 ◽  
Author(s):  
Muendo Charity Mutile ◽  
Mutiso Joshua Muli ◽  
Gicheru Michael Muita
Keyword(s):  
Visceral Leishmaniasis ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Prosopis Juliflora ◽  
In Vitro Test ◽  
Reference Drug ◽  
Major Health Problem ◽  
Group I ◽  
Vitro Test

Background: Visceral Leishmaniasis caused by Leishmania donovani is a major health problem in the tropics and sub-tropic regions where it is endemic. We aimed in testing the leishmanicidal activity and toxicity of Prosopis juliflora leaf extract in BALB/c mice and in vitro test systems respectively. Methods: In the year 2017 until 2019, BALB/c mice of mixed sexes aged between 6 and 8 weeks in groups of 8 were used. Group I treated with 100 mg/kg of P. juliflora extract, Group II -1 mg/kg of Sodium stibogluconate (SSG) and Group III treated with normal saline. All mice were anaesthized and sacrificed to obtain blood, spleen samples for antibody measurements, and determination of parasite loads. Results: There was significant inhibitory effect (P<0.05) exhibited by P. juliflora leaf extract on promastigote growth during the in vitro test whereby up to 98% parasites were killed at the highest concentrations of 100 µg/Ml of the extract as compared to SSG, which showed less inhibitory effect on promastigotes. P. juliflora exhibited a higher splenic antiamastigote effect after 21 days of administration as compared to SSG. P. juliflora methanolic leaf extract induced a higher total IgG level as compared to the reference drug which could be attributed to higher titer in IgG2a subtype in mice treated with the extract, which was not induced in mice, treated with SSG. Conclusion: P. juliflora exhibited higher inhibitory effects against L. donovani promastigotes as well as amastigotes and induced significantly higher IgG antibody levels as compared to SSG (P<0.05). Furthermore, it was safer than SSG on Vero E6 cells.

Haemostatic Platelet Plug Formation in the Isolated Rabbit Mesenteric Preparation - An Analysis of Red Blood Cell Participation

1980 ◽  
Vol 44 (01) ◽  
pp. 006-008 ◽  
Author(s):  
D Bergqvist ◽  
K-E Arfors
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
In Vitro Test ◽  
Pharmacological Agents ◽  
Vitro Test ◽  
Releasing Effect ◽  
Plug Formation

SummaryIn a model using an isolated rabbit mesenteric preparation microvessels were transected and the time until haemostatic plugs formed was registered. Perfusion of platelet rich plasma gave no haemostasis whereas whole blood did. Addition of chlorpromazine or adenosine to the whole blood significantly prolonged the time for haemostasis, and addition of ADP to the platelet rich plasma significantly shortened it. It is concluded that red cells are necessary for a normal haemostasis in this model, probably by a combination of a haemodynamic and ADP releasing effect.The fundamental role of platelets in haemostatic plug formation is unquestionable but there are still problems concerning the stimulus for this process to start. Three platelet aggregating substances have been discussed – thrombin, adenosine diphosphate (ADP) and collagen. Evidence speaking in favour of thrombin is, however, very minimal, and the discussion has to be focused on collagen and ADP. In an in vitro system using polyethylene tubings we have shown that "haemostasis" can be obtained without the presence of collagen but against these results can be argued that it is only another in vitro test for platelet aggregation (1).To be able to induce haemostasis in this model, however, the presence of red blood cells is necessary. To further study this problem we have developed a model where haemostatic plug formation can be studied in the isolated rabbit mesentery and we have briefly reported on this (2).Thus, it is possible to perfuse the vessels with whole blood as well as with platelet rich plasma (PRP) and different pharmacological agents of importance.

scholarly journals In Vitro Newly Isolated Environmental Phage Activity against Biofilms Preformed by Pseudomonas aeruginosa from Patients with Cystic Fibrosis

2021 ◽  
Vol 9 (3) ◽  
pp. 478
Author(s):  
Ersilia Vita Fiscarelli ◽  
Martina Rossitto ◽  
Paola Rosati ◽  
Nour Essa ◽  
Valentina Crocetta ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
In Vitro Test ◽  
Chronic Infections ◽  
Hospital Environments ◽  
Vitro Test ◽  
General Reliability ◽  
Phage Activity

As disease worsens in patients with cystic fibrosis (CF), Pseudomonas aeruginosa (PA) colonizes the lungs, causing pulmonary failure and mortality. Progressively, PA forms typical biofilms, and antibiotic treatments determine multidrug-resistant (MDR) PA strains. To advance new therapies against MDR PA, research has reappraised bacteriophages (phages), viruses naturally infecting bacteria. Because few in vitro studies have tested phages on CF PA biofilms, general reliability remains unclear. This study aimed to test in vitro newly isolated environmental phage activity against PA isolates from patients with CF at Bambino Gesù Children’s Hospital (OBG), Rome, Italy. After testing in vitro phage activities, we combined phages with amikacin, meropenem, and tobramycin against CF PA pre-formed biofilms. We also investigated new emerging morphotypes and bacterial regrowth. We obtained 22 newly isolated phages from various environments, including OBG. In about 94% of 32 CF PA isolates tested, these phages showed in vitro PA lysis. Despite poor efficacy against chronic CF PA, five selected-lytic-phages (Φ4_ZP1, Φ9_ZP2, Φ14_OBG, Φ17_OBG, and Φ19_OBG) showed wide host activity. The Φ4_ZP1-meropenem and Φ14_OBG-tobramycin combinations significantly reduced CF PA biofilms (p < 0.001). To advance potential combined phage-antibiotic therapy, we envisage further in vitro test combinations with newly isolated phages, including those from hospital environments, against CF PA biofilms from early and chronic infections.

In Vitro Toxicology Methods in Risk Assessment

1996 ◽  
Vol 24 (3) ◽  
pp. 325-331
Author(s):  
Iain F. H. Purchase
Keyword(s):  
Risk Assessment ◽  
Hazard Assessment ◽  
Human Health Risk ◽  
In Vitro Test ◽  
In Vitro Methods ◽  
New Concepts ◽  
Vitro Test

The title of this paper is challenging, because the question of how in vitro methods and results contribute to human health risk assessment is rarely considered. The process of risk assessment usually begins with hazard assessment, which provides a description of the inherent toxicological properties of the chemical. The next step is to assess the relevance of this to humans, i.e. the human hazard assessment. Finally, information on exposure is examined, and risk can then be assessed. In vitro methods have a limited, but important, role to play in risk assessment. The results can be used for classification and labelling; these are methods of controlling exposure, analogous to risk assessment, but without considering exposure. The Ames Salmonella test is the only in vitro method which is incorporated into regulations and used widely. Data from this test can, at best, lead to classification of a chemical with regard to genotoxicity, but cannot be used for classification and labelling on their own. Several in vitro test systems which assess the topical irritancy and corrosivity of chemicals have been reasonably well validated, and the results from these tests can be used for classification. The future development of in vitro methods is likely to be slow, as it depends on the development of new concepts and ideas. The in vivo methods which currently have reasonably developed in vitro alternatives will be the easiest to replace. The remaining in vivo methods, which provide toxicological information from repeated chronic dosing, with varied endpoints and by mechanisms which are not understood, will be more difficult to replace.

scholarly journals In vitro dissolution testing models of ocular implants for posterior segment drug delivery

2021 ◽  
Author(s):  
Muhammad Faris Adrianto ◽  
Febri Annuryanti ◽  
Clive G. Wilson ◽  
Ravi Sheshala ◽  
Raghu Raj Singh Thakur
Keyword(s):  
Drug Release ◽  
Posterior Segment ◽  
In Vitro Dissolution ◽  
Prolonged Treatment ◽  
Term Therapy ◽  
In Vitro Test ◽  
Dissolution Testing ◽  
Vitro Test ◽  
Ocular Implants

AbstractThe delivery of drugs to the posterior segment of the eye remains a tremendously difficult task. Prolonged treatment in conventional intravitreal therapy requires injections that are administered frequently due to the rapid clearance of the drug molecules. As an alternative, intraocular implants can offer drug release for long-term therapy. However, one of the several challenges in developing intraocular implants is selecting an appropriate in vitro dissolution testing model. In order to determine the efficacy of ocular implants in drug release, multiple in vitro test models were emerging. While these in vitro models may be used to analyse drug release profiles, the findings may not predict in vivo retinal drug exposure as this is influenced by metabolic and physiological factors. This review considers various types of in vitro test methods used to test drug release of ocular implants. Importantly, it discusses the challenges and factors that must be considered in the development and testing of the implants in an in vitro setup. Graphical abstract

Animal Models and Alternatives in the Quality Control of Vaccines: Are In Vitro Methods or In Vivo Methods the Scientific Equivalent of the Emperor's New Clothes?

1995 ◽  
Vol 23 (1) ◽  
pp. 61-73
Author(s):  
Coenraad Hendriksen ◽  
Johan van der Gun
Keyword(s):  
Quality Control ◽  
Test Methods ◽  
In Vitro Test ◽  
Large Numbers ◽  
Alternative Approach ◽  
Inactivated Vaccines ◽  
Vitro Test

In the quality control of vaccine batches, the potency testing of inactivated vaccines is one of the areas requiring very large numbers of animals, which usually suffer significant distress as a result of the experimental procedures employed. This article deals with the potency testing of diphtheria and tetanus toxoids, two vaccines which are used extensively throughout the world. The relevance of the potency test prescribed by the European Pharmacopoeia monographs is questioned. The validity of the potency test as a model for the human response, the ability of the test to be standardised, and the relevance of the test in relation to the quality of the product are discussed. It is concluded that the potency test has only limited predictive value for the antitoxin responses to be expected in recipients of these toxoids. An alternative approach for estimating the potency of toxoid batches is discussed, in which a distinction is made between estimation of the immunogenic potency of the first few batches obtained from a seed lot and monitoring the consistency of the quality of subsequent batches. The use of animals is limited to the first few batches. Monitoring the consistency of the quality of subsequent batches is based on in vitro test methods. Factors which hamper the introduction and acceptance of the alternative approach are considered. Finally, proposals are made for replacement, reduction and/or refinement (the Three Rs) in the use of animals in the routine potency testing of toxoids.

Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials

2006 ◽  
Vol 69 (12) ◽  
pp. 976-982 ◽  
Author(s):  
Nenad Ignjatović ◽  
Petar Ninkov ◽  
Vesna Kojić ◽  
Miloš Bokurov ◽  
Vladimir Srdić ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
In Vitro Test ◽  
Vitro Test
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