Fe induced a dynamic and biased allocation of material flux within terpenoid metabolism is controlled by CbNudix in Conyza blinii
Abstract Conyza Blinii (C. blinii) is a unique traditional Chinese medicine growing in Sichuan, China, which has soils with an abundant iron content. This Fe enrichment environment contributed to a variety of terpenoids of C. blinii, blinin and saponin which can play an important role in the process of resisting abiotic stress. The relationship between Fe and terpenoid metabolism was studied to explore Fe tolerance mechanism of C. blinii. In this study, C. blinii was treated with ferrous iron solutions, and the effect of ferrous iron on the synthesis of blinin and saponins was further studied by spectrophotometry and liquid chromatography. Also, gene expression was detected by qRT-PCR. Under ferrous treatment, the blinin content of C. blinii increased, while the total saponin content decreased. When the ferrous concentration reached 200 μM, the difference in metabolite production was the largest. Furthermore, it was found that blinin and saponin have synchronous and opposite accumulation trends, accompanied by time dependence. The genes expression results of key enzyme in the MVA and MEP pathways showed the same trends. In this process, the expression of CbNudixs played a key role to switch the material flux between MVA and MEP, via catalyzed the dephosphorylation of isoprenoid diphosphate. This study explored the effect of ferrous iron on the saponin and blinin synthesis of C. blinii and found the unique role of CbNudixs in regulating material flux between diterpene and triterpene synthesis, which greatly deepened our understanding of the mechanism of Fe tolerance of C. blinii.