Antimicrobial Activities of Coriander Seed Essential Oil and Silver Nanoparticles

Author(s):  
Kadriye Ozlem saygi ◽  
Birgul Kacmaz ◽  
Serdar Gul

Abstract Bacterial infections are one of the most serious health problems all over the world, which cause need for the discovery of new drug. Since antibiotic resistance is a major threat to both humans and the environment, there is a need for studies on the antimicrobial properties of different forms of traditionally used plants. Herein, the seeds of coriander were used to isolate essential oil (EO) and to synthesise silver nanoparticles (C-AgNPs). The major oil constituents were characterized by GC-MS as Linalool (79.12%), Camphor (6.16%), γ-Terpinene (2.82%) and α-Pinene (2.67%). The synthesized AgNPs were characterized by UV-Visible spectrophotometry, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), X-ray difraction (XRD) and Fourier Tranmission Infrared (FTIR). The surface plasmon resonance (SPR) of C-AgNPs at 437 nm was recorded on the UV-Vis spectrometer. The spherical and homogenous of AgNPs was presented in SEM images. EDX showed elemental distribution and confirmed AgNPs. A characteristic intense peak was at 3.0 keV. The antibacterial activities of the essential oil and AgNPs form of coriander seed against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922 bacteria were investigated by broth microdilution test. AgNPs and essential oil of coriander can be expected to provide future opportunities in nanomedicine and materials science. AgNPs can be displayed synergistic antimicrobial effect when used in combination with essential oil.

Author(s):  
Judith M. Brock ◽  
Max T. Otten

A knowledge of the distribution of chemical elements in a specimen is often highly useful. In materials science specimens features such as grain boundaries and precipitates generally force a certain order on mental distribution, so that a single profile away from the boundary or precipitate gives a full description of all relevant data. No such simplicity can be assumed in life science specimens, where elements can occur various combinations and in different concentrations in tissue. In the latter case a two-dimensional elemental-distribution image is required to describe the material adequately. X-ray mapping provides such of the distribution of elements.The big disadvantage of x-ray mapping hitherto has been one requirement: the transmission electron microscope must have the scanning function. In cases where the STEM functionality – to record scanning images using a variety of STEM detectors – is not used, but only x-ray mapping is intended, a significant investment must still be made in the scanning system: electronics that drive the beam, detectors for generating the scanning images, and monitors for displaying and recording the images.


Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1716 ◽  
Author(s):  
Muna Ali ◽  
Kareem Mosa ◽  
Ali El-Keblawy ◽  
Hussain Alawadhi

Nanoparticle (NP) synthesis by biological systems is more cost-effective, safe, and environmentally friendly when compared to currently used chemical and physical methods. Although many studies have utilized different plant extracts to synthesize NPs, few studies have incorporated living plants. In this study, silver nanoparticles (AgNPs) were synthesized exogenously by Tephrosia apollinea living plant system under the combined stresses of silver nitrate and different levels of drought stress simulated by Polyethylene glycol (PEG) (0, −0.1, −0.2, and −0.4 MPa for three and six days). Biomass, cell death, and H2O2 content were evaluated to determine the toxicological effect of the treatments on the plant. More severe effects were detected in day 6 plants compared to day 3 plants, and at higher drought levels. UV-visible spectrum, energy dispersive X-ray spectroscopy, X-ray diffraction, scanning electron microscope, and Fourier transform infrared spectroscopy were used to detect and characterize the T. apollinea synthesized NPs. The shapes of the NPs were spherical and cubic with different phytochemicals being the possible capping agents. Broth microdilution was used to determine the antimicrobial activity of the NPs against Escherichia coli and Staphylococcus aureus. In this case, antimicrobial activity increased at higher PEG concentrations. Bactericidal effects were observed against E. coli, while only bacteriostatic effects were detected against S. aureus.


Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1512 ◽  
Author(s):  
Jeong Su Choi ◽  
Ji Woong Lee ◽  
Un Chul Shin ◽  
Min Woo Lee ◽  
Dae Jin Kim ◽  
...  

This paper investigated the antifungal and antibiofilm activity of silver nanoparticles synthesized with Lycopersicon esculentum extracts against Candida species. Lycopersicon esculentum extracts obtained by homogenization were mixed with silver nitrate to synthesize silver nanoparticles. Analysis of the particle characteristics by UV–Vis spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDAX), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FT-IR) confirmed that the Lycopersicon esculentum extracts effectively served as reductants and capping agents. Minimum inhibitory concentration (MIC) tests were conducted to confirm antifungal activity against Candida species. In all the tested species, the silver nanoparticles inhibited the growth of Candida. Moreover, the SEM images of Candida species treated with silver nanoparticles synthesized using natural extracts of Lycopersicon esculentum showed that silver nanoparticles adhered to the surface of Candida, which induced pore formation in the membranes and prevented their normal growth. Ultimately, these abnormal forms of Candida were thought to be less able to form biofilms than normal Candida. The antifungal and antibiofilm activities of silver nanoparticles against Candida are expected to be utilized in various fields and contribute in particular to developments in nanomedicine.


2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Musarat Amina ◽  
Nawal M. Al Musayeib ◽  
Nawal A. Alarfaj ◽  
Maha F. El-Tohamy ◽  
Hisham E. Orabi ◽  
...  

The present study focused on the prospect of fabricating a polymeric naturally extracted Moringa oleifera oil bionanocomposite film enriched with silver nanoparticles for antimicrobial activity. In this study, a standard concentration of Moringa oleifera oil (5-10 wt%) was used to fabricate a polymeric bionanocomposite film using polyvinyl chloride (PVC) enriched with silver nanoparticles. The active constituents of the extracted Moringa oleifera oil were verified using gas chromatography-mass spectrometry. Spectroscopic and microscopic techniques, including scanning electron microscopy, X-ray diffraction, and energy-dispersive X-ray analysis, were employed to characterize and study the surface morphology of the fabricated bionanocomposite film. The antimicrobial activity of the fabricated bionanocomposite film was investigated using different strains of bacteria and fungus. The results revealed well-oriented and excellently dispersed silver nanoparticles in the PVC-Moringa oleifera oil matrix. The bionanocomposite was able to inhibit the growth of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa, Shigella flexneri, and Candida albicans. The combination of nanoparticles with polymers is opening new routes for engineering fixable composites, which showed antimicrobial properties.


Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1775 ◽  
Author(s):  
Raphaël E. Duval ◽  
Jimmy Gouyau ◽  
Emmanuel Lamouroux

Due to the constant increase in the number of infectious diseases and the concomitant lack of treatment available, metallic nanoparticles (e.g., silver nanoparticles) have been of particular interest in the last decades. Indeed, several studies suggest that silver nanoparticles have valuable antimicrobial activities, especially against bacteria, which may lead us to think that these nanoparticles may one day be an attractive therapeutic option for the treatment of bacterial infections. Unfortunately, when we look a little closer to these studies, we can see a very great heterogeneity (e.g., in the study design, in the synthetic process of nanoparticles, in the methods that explore the antibacterial properties of nanoparticles and in the bacteria chosen) making cross-interpretation between these studies impossible, and significantly limiting the interest of silver nanoparticles as promising antibacterial agents. We have selected forty-nine international publications published since 2015, and propose to discuss, not the results obtained, but precisely the different methodologies developed in these publications. Through this discussion, we highlighted the aspects to improve, or at least to homogenize, in order to definitively establish the interest of silver nanoparticles as valuable antibacterial agents.


Author(s):  
ALEXANDRE PORTE ◽  
RONOEL LUIZ DE OLIVEIRA GODOY

Apresenta breve revisão bibliográfica sobre a composição química e as propriedades antimicrobianas do óleo essencial de alecrim. Enfoca aspectos gerais, usos, atividade contra bactérias e fungos em alimentos e as principais substâncias do óleo de alecrim oriundo de diferentes países. Conclui que os compostos oxigenados são os responsáveis pelas atividades fisiológica e antimicrobiana, mas há discordância sobre os compostos majoritários, ora hidrocarbonetos ora oxigenados. ROSEMARY (Rosmarinus officinalis L.): ESSENTIAL OIL ANTIMICROBIAL AND CHEMICAL PROPERTIES Abstract This article presents a brief review on the chemical composition and antimicrobial properties of the rosemary essential oil. It focuses general features, uses, antimicrobial activity against bacteria and fungi in foods and the major compounds of the rosemary oil from different countries. Concludes that physiologic and antimicrobial activities are due to oxygenated compounds, but there is discordance about the major components if hydrocarbon or oxygenated monoterpenes.


Author(s):  
Tevan R ◽  
Saravanan Jayakumar ◽  
Nor Haledah Ahmad Sahimi ◽  
Nur Farah Ain Iqbal ◽  
Iffah Zapri ◽  
...  

Biosynthesis of metal nanoparticles has received a remarkable attention due to their eco-friendly and potential applications in pharmaceutical and medical fields. The searches for natural alternatives to replace biosynthetic nanoparticles have resulted in extensive studies of microalgal derived metal nanoparticles. Since there are very limited reports on Isochrysis sp. in synthesising metal nanoparticles, a novel initiative was taken to induce an environmentally friendly and low cost technique to biosynthesise the silver nanoparticles (AgNPs) using marine microalgae, Isochrysis sp. Further, the synthesised silver nanoparticles were screened against human pathogen for antimicrobial effects. The characterisation of nanoparticles were confirmed by UV visible spectroscopy, field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (XRD). The results obtained from characterisations indicate that the AgNPs have an almost spherical shape with a various size of 98.1 to 193 nm. The synthesised nanoparticles exhibited outstanding antioxidant and antimicrobial activities.


Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 212
Author(s):  
Hemmat M. Abd-Elhady ◽  
Mona A. Ashor ◽  
Abdelkader Hazem ◽  
Fayez M. Saleh ◽  
Samy Selim ◽  
...  

The ability of microorganisms to reduce inorganic metals has launched an exciting eco-friendly approach towards developing green nanotechnology. Thus, the synthesis of metal nanoparticles through a biological approach is an important aspect of current nanotechnology. In this study, Streptomyces aizuneusis ATCC 14921 gave the small particle of silver nanoparticles (AgNPs) a size of 38.45 nm, with 1.342 optical density. AgNPs produced by Streptomyces aizuneusis were characterized by means of UV-VIS spectroscopy and transmission electron microscopy (TEM). The UV-Vis spectrum of the aqueous solution containing silver ion showed a peak between 410 to 430. Moreover, the majority of nanoparticles were found to be a spherical shape with variables between 11 to 42 nm, as seen under TEM. The purity of extracted AgNPs was investigated by energy dispersive X-ray analysis (EDXA), and the identification of the possible biomolecules responsible for the reduction of Ag+ ions by the cell filtrate was carried out by Fourier Transform Infrared spectrum (FTIR). High antimicrobial activities were observed by AgNPs at a low concentration of 0.01 ppm, however, no deleterious effect of AgNPs was observed on the development and occurrence of Drosophila melanogaster phenotype. The highest reduction in the viability of the human lung carcinoma and normal cells was attained at 0.2 AgNPs ppm.


Author(s):  
Hussein A. R. Abbood ◽  
Salah S. Zain alabden

This article aims to detect the effect of combining silver nanoparticles prepared with the extract of the Pimpinella anisum seeds and was obtained from the local markets in the city of Kirkuk. Iraq with some antibiotics on antibiotic resistance bacteria (E.coli and Staphylococcus aureus ) were obtained from Kirkuk General Hospital and re-diagnosed based on reliable diagnostic sources. This study demonstrates that the P. anisum seeds extract reduced the silver ion through changing the solution color to reddish brown. The examination of silver nanoparticles synthesized by P. anisum the extract of seeds was carried out using X-ray and SEM (Scanning electron microscopy) and X-ray showed spherical shape particles with 44 nm in size with three diffractions which are indexed as (111), (200) and (220)and the SEM images showed clear particales. The synergistic effects of biosynthesis AgNPs at different concentrations with different standard antibiotic discs (Amoxicillin, chloramphenicol, Erythromycin, Ciprofloxacin) against multi-antibiotic resistance bacteria were also investigated. The result showed the synergistic action of AgNPs and antibiotics leading to enhance antibacterial activity.


Author(s):  
Derya Doğanay ◽  
Sevval M. Özcan ◽  
Ahmet M. Şentürk ◽  
Süreyya Ölgen

Background: Besides the viral infections, bacterial infections can cause serious and life-threatening complications and drug resistance is an important problem to fight bacterial infections. Therefore, it is important to discover novel antimicrobial agents to fight such infections. Objective: Several indole containing antimicrobial drug development studies have been reported in literature that provided strong evidences for good antimicrobial activities against a variety of microorganisms. Taken into consideration from these findings, antimicrobial properties of previously synthesized 16 indole amide derivatives were evaluated by in vitro tests against 14 different microorganisms, and also molecular docking and in silico prediction studies were used to identify structure-activity relationship of compounds. Methods: Antimicrobial activity of compounds was determined by disc diffusion and tube dilution methods. Molecular docking of compounds was studied to determine the relationship between the structure of compounds with DNA gyrase interactions of microorganisms by using the version of Autodock vina 4.2.6. Mol inspiration and Swiss ADME prediction online software programs were also used to identify drug-like properties of compounds. Results: The results showed that some compounds exhibited quite pronounced antibacterial and antifungal activities compared to reference drugs. These results were also supported by molecular docking studies and in silico ADME calculations presented that all tested compounds obey the Lipinski’s Rule of Five and are metabolized by CYP450 enzymes. Conclusion: It can be concluded that these results can be taken as reference in the development of new indole-based antimicrobial agents.


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