Hydrotropic Root Behavior in Water-Saving Cultivation: A High-Resolution Monitoring Study of Soil Water Dynamics
Abstract BackgroundSubsurface irrigation has been confirmed to have high water use efficiency due to it irrigating only the crop root zone. Hydrotropism allows roots to grow towards higher water content areas for drought avoidance, which has research interests in recent years. However, most hydrotropism studies focused on a single root and were conducted in air or agar systems. The performance of hydrotropism in subsurface irrigation is not clear. ResultsWe developed a method to observe and analyze hydrotropism in soil under water-saving cultivation. A wet zone was produced around the whole root system based on using subsurface irrigation method and micro soil water dynamics were observed using high-resolution soil moisture sensors. This method enabled the observation and analysis of plant water absorption activities and the hydrotropic response of the root system. In the analysis, we first applied a high-pass filter and fast Fourier transform to the soil water dynamics data. The results indicated that the plant’s biological rhythm of photosynthetic activities can be identified from the soil moisture data. We then observed root growth in response to the dynamics of soil water content in the wet zone. We quantified root distribution inside and outside the wet zone and observed the shape of the root system from the cross-section of the wet zone. The results showed that the root hydrotropic response is not uniform for all roots of an individual plant. ConclusionsThis study verified the feasibility of using high-resolution soil moisture sensors to study root hydrotropic responses in soil during water-saving cultivation. To further evaluate a plant’s hydrotropic ability, it is necessary to use statistical analysis and/or a non-deterministic approach. Future studies may also explore developing an automated experimental system and robotic manipulations for getting steady repeatable observation of hydrotropism in water-saving cultivation.