scholarly journals Osmotic Seed Priming of Rudbeckia fulgida Improves Germination and Expands Germination Range

HortScience ◽  
1994 ◽  
Vol 29 (8) ◽  
pp. 868-870 ◽  
Author(s):  
Amy M. Fay ◽  
Mark A. Bennett ◽  
Steven M. Still
Keyword(s):  
Polyethylene Glycol ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
The Mean ◽  
Mean Time

Low-vigor seeds of black-eyed Susan (Rudbeckia fulgida Ait.) primed in aerated -1.3 MPa KNO3 for 7 days at 30C in darkness had double the total germination percentage at 30C and one-half the mean time of germination as nonprimed seeds. Priming the seeds in polyethylene glycol rather than KNO3 generally resulted in lower total germination percentage and longer mean time of germination. Osmotic priming increased total germination percentage and germination rate of seeds germinated at 21.9 to 32.2C, but the priming benefit on total germination percentage was greater at ≤27.6C. Total germination percentage of primed and nonprimed seeds was highest at 28.8 to 32.2C.

scholarly journals 837 PB 355 A COMPARISON OF BROCCOLI SEED PRIMING TREATMENTS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 553b-553
Author(s):  
Lewis W. Jett ◽  
Gregory E. Welbaum
Keyword(s):  
Polyethylene Glycol ◽  
Calcium Silicate ◽  
Priming Effect ◽  
Field Experiments ◽  
Solid Phase ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
The Mean ◽  
Mean Time

Various osmotic solutions and solid carriers have been used to prime (controlled hydration followed by drying) seeds. Broccoli (Brassica oleracea L., var. italica, cvs. Brigadier and Earlidawn) seeds were primed in solutions of polyethylene glycol, potassium nitrate (KNO3), and mannitol or calcium silicate (Micro-ccl E) and vermiculite to determine which treatment provided the greatest enhancement of germination. Germination percentage and rate as well as head yield were determined in a series of laboratory, glasshouse, and field experiments over a three year period. Water potentials (Y) ranged from -0.8 to -2.6 MPa at 20°C for both osmotic solutions and carriers. The duration of the priming treatments were 3, 7, or 10 days. All priming treatments, except KNO3 and mannitol, consistently reduced the mean time to germination in the laboratory, reduced the mean time to field emergence, increased final stands, but did not affect the mean time to harvest. However, solid phase was more effective than osmotic priming at each Ψ tested. The best solid priming mixture was 1.0:0.8:1.8 (seed:carrier:water) for 7 days at 20°C using Micro-cel E. This treatment was measured to have a Y = -1.2 MPa, and other types of priming at the same Y were less effective. The Y threshold below which no priming effect was obtained was Ψ= -2.4 MPa. Priming response was closely associated with, but not entirely dependent on, the Ψ of the treatment.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Does Primed Seed Improve Stand Establishment and Yield of Broccoli?

1995 ◽  
Vol 5 (4) ◽  
pp. 314-317 ◽  
Author(s):  
Lewis W. Jett ◽  
Gregory E. Welbaum ◽  
Charles R. O'Dell ◽  
Ronald D. Morse
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycol ◽  
Brassica Oleracea ◽  
Seedling Emergence ◽  
Plant Competition ◽  
Seed Priming ◽  
Stand Establishment ◽  
The Third ◽  
The Mean ◽  
Mean Time

The effect of matric and osmotic seed priming on stand establishment and maturity of broccoli (Brassica oleracea L. var. italica) was investigated in three years at two locations in Virginia. Seeds (`Earlidawn') were primed at 1.1 MPa (68F for 7 days) either osmotically in polyethylene glycol (8000 molecular weight) or metrically in vermiculite (horticultural grade no. 2). In the frost year of the study, seeds were hand-seeded in August into crustprone soil with a mean temperature of 82F, and there were no differences in the percentage or mean time of seedling emergence between osmotic- and matric-primed seeds. Under cooler temperatures during the remaining two years of the study, priming increased the percent emergence and decreased the mean time of emergence by about 15 hours. Primed seeds did not increase yields or accelerate maturity in two out of three years. In the third year, the spread of seedling emergence times was less for primed seeds, which reduced plant-to-plant competition and hastened maturity. The primary benefit of primed broccoli seeds was faster emergence, which increased stands by reducing exposure to stresses that decrease emergence.

scholarly journals Effect of hydration treatments on seed parameters of different lettuce (Lactuca sativa L.) seed lots

2012 ◽  
Vol 29 (No. 1) ◽  
pp. 12-16
Author(s):  
J. Pazdera ◽  
V. Hosnedl
Keyword(s):  
Polyethylene Glycol ◽  
Germination Percentage ◽  
Lettuce Seed ◽  
Sufficient Criterion ◽  
Lactuca Sativa L ◽  
Treated Seed ◽  
The Mean ◽  
Mean Time ◽  
Influence Of Hydration

The influence of hydration treatments on seed parameters of lettuce seed lots with different initial quality was investigated. Nine seed lots were treated by prehydration (for 3, 6, 12 and 24 hours) and by osmopriming in polyethylene glycol 6,000 solution (for 24, 72 and 144 hours) with osmotic potential –1.5 MPa. The result of hydration treatment depends on seed lot attributes. The germination percentage is not a sufficient criterion for determination of seed suitability for hydration treatment. A possible complementary parameter can be the mean time of germination (MTG) with high correlation between untreated and treated seed lots. The effect of hydration also depends on the hydration method and time of treatment. The osmopriming is a more suitable method for lettuce seed than prehydration. Generally, a shorter time of hydration (till 6 hours at prehydration and till 72 hours at priming) proved more effective on the parameters of lettuce seed lots after treatment.

scholarly journals Effects of Matric and Osmotic Priming Treatments on Broccoli Seed Germination

1996 ◽  
Vol 121 (3) ◽  
pp. 423-429 ◽  
Author(s):  
Lewis W. Jett ◽  
Gregory E. Welbaum ◽  
Ronald D. Morse
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Root Growth ◽  
Growth Rates ◽  
Germination Rate ◽  
Membrane Integrity ◽  
Seed Vigor ◽  
Radicle Emergence ◽  
The Mean ◽  
Peg 8000

Priming, a controlled-hydration treatment followed by redrying, improves the germination and emergence of seeds from many species. We compared osmotic and matric priming to determine which was the most effective treatment for improving broccoli seed germination and to gain a greater understanding of how seed vigor is enhanced by priming. Broccoli (Brassica oleracea L. var. italica) seeds were osmotically primed in polyethylene glycol (PEG 8000) at -1.1 MPa or matrically primed in a ratio of 1.0 g seed:0.8 g synthetic calcium silicate (Micro-Cel E):1.8 ml water at -1.2 MPa. In the laboratory, germination rates and root lengths were recorded from 5 to 42C and 10 to 35C, respectively. Broccoli seeds germinated poorly at >35C. Root growth after germination was more sensitive to temperatures >30C and <15C than radicle emergence. Matric and osmotic priming increased germination rate in the laboratory, greenhouse, and field. However, matric priming had a greater effect on germination and root growth rates from 15 to 30C. Neither priming treatment affected minimum or maximum germination or root growth temperatures. Both priming treatments decreased the mean thermal time for germination by >35%. The greater germination performance of matrically primed seeds was most likely the result of increased oxygen availability during priming, increased seed Ca content, or improved membrane integrity.

PARTICIPATORY EVALUATION BY FARMERS OF ON-FARM SEED PRIMING IN WHEAT IN INDIA, NEPAL AND PAKISTAN

2001 ◽  
Vol 37 (3) ◽  
pp. 403-415 ◽  
Author(s):  
D. Harris ◽  
B. S. Raghuwanshi ◽  
J. S. Gangwar ◽  
S. C. Singh ◽  
K. D. Joshi ◽  
...  
Keyword(s):  
Seed Priming ◽  
Germination Percentage ◽  
Wheat Seed ◽  
Saline Irrigation ◽  
Yield Increase ◽  
Marginal Areas ◽  
Input Use ◽  
On Farm ◽  
Tribal Areas ◽  
Mean Time

The mean time for 50% germination at 20 °C of 12 Indian wheat (Triticum aestivum) cultivars was nearly halved, from 51 h to 27 h, by soaking seed in water for 8 h prior to sowing. A delay of 24 h without further soaking, intended to simulate postponement of sowing, reduced the time saved by priming to 16%. Priming had no effect on final germination percentage. These results were used in the design of 275 on-farm, farmer-managed, participatory trials of seed priming in wheat during the 1997–98 and 1998–99 rabi (post-monsoon) seasons. In forty-one trials in tribal areas of Bihar and West Bengal states of India, seed priming gave a 13% grain yield advantage for farmers growing wheat in marginal areas with low levels of agricultural inputs. Mean benefits from seed priming of wheat in nine trials in Chitwan, Nepal were 17%. In high potential areas of Gujarat, India, 205 trials had higher rates of input use. Yield benefit from priming in these trials averaged only 5% but constituted an extra 200 kg ha−1 grain at little or no cost. In 20 trials on marginal land, with slightly saline irrigation water in Ahmadwala, Pakistan, an average yield increase of 36% was obtained using seed primed with a 0.2% gypsum solution. Collaborating farmers reported that priming wheat seed overnight resulted in faster, more complete emergence, more vigorous early growth, better tillering, earlier flowering, larger ears, earlier maturity and higher yields. In addition, many farmers also reported that foliage in primed plots was a darker shade of green than that in non-primed plots, suggesting that primed plants may have been using nitrogen more efficiently. Seed priming was popular with farmers, most of whom reported that they would prime wheat seed the following year. A survey in Gujarat in 1998–99 of 63 farmers who had tested priming in 1997–98 showed that, while 65% had primed some of their own seed, none had primed more than 50 kg, suggesting that there were practical difficulties in priming larger volumes of seed.

scholarly journals Effect of Seed Priming on Germination Properties and Seedling Establishment of Cowpea (Vigna sinensis)

2011 ◽  
Vol 3 (4) ◽  
pp. 113-116 ◽  
Author(s):  
Hamdollah ESKANDARI ◽  
Kamyar KAZEMI
Keyword(s):  
Laboratory Tests ◽  
Low Cost ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Laboratory Data ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Vigor ◽  
Contributing Factors

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.

scholarly journals Priming and Accelerated Aging Affect L-isoaspartyl Methyltransferase Activity in Tomato Seed

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 632d-632 ◽  
Author(s):  
S.T. Kester ◽  
R.L. Geneve ◽  
R.L. Houtz
Keyword(s):  
Accelerated Aging ◽  
Germination Percentage ◽  
Methyltransferase Activity ◽  
Tomato Seed ◽  
Developmentally Regulated ◽  
New Yorker ◽  
Radicle Emergence ◽  
The Mean ◽  
Mean Time ◽  
Isoaspartyl Methyltransferase

Damage and degradation of cellular proteins is observed during seed deterioration due to aging. L-isoaspartyl methyltransferase (EC 2.1.1.77) is an enzyme hypothesized to play a role in limiting and repairing aging-induced damage of proteins. Tomato seeds (Lycopersicon esculentum `New Yorker') were assayed for changes in L-isoaspartyl methyltransferase activity during accelerated aging and after osmotic priming. Accelerated aging of seeds for 1 to 4 days at 45°C and 100% humidity reduced germination from 94% to 71%, the mean time of germination (MTG) increased from 2.4 to 5.8 days and was accompanied by a correlative decrease in L-isoaspartyl methyltransferase activity R2 = 0.90. Aged and untreated seeds were primed for 7 days at 20°C in darkness using aerated solutions of 3% KNO3 or polyethylene glycol 8000 (PEG) with equivalent osmotic potential (–1.25 MPa). Priming with KNO3 decreased the MTG but not germination percentage for untreated seeds. Priming did not affect L-isoaspartyl methyltransferase activity in untreated seeds but restored activity in aged seeds primed in KNO3 to levels near that of untreated seeds. Priming with PEG did not effectively improve the MTG or increase L-isoaspartyl methyltransferase activity. During germination, enzyme activity remained constant for 48 hours post-imbibition and then declined suggesting the enzyme was developmentally regulated and inactivated or degraded as radicle emergence occurred.

scholarly journals Physiological quality of osmoprimed Eremanthus erythropappus (D.C.) MacLeish seeds

2020 ◽  
Vol 13 (5) ◽  
pp. 18
Author(s):  
A. M. S. Oliveira ◽  
A. A. Silva ◽  
M. C. Vasconcelos ◽  
J. A. A. Granja ◽  
J. M. R. Faria ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Germination Rate ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
The Other ◽  
Other Substances ◽  
Physiological Quality ◽  
Glycol Solution ◽  
Polyethylene Glycol 6000

Physiological conditioning is a technique that consists in controlling the speed of water uptake by seeds, with osmotic solutions, aiming to improve their quality, reducing time and increasing germination rate. Eremanthus erythropappus (D.C.) MacLeish seeds were subjected to priming with polyethylene glycol 6000 (PEG), potassium nitrate (KNO3) and the combination of the two at the concentrations of 0.4; -0.8; -1.0; -1.2 and -1.4 MPa for four, six and eight days. Germination, germination speed index (GSI), emergence, emergence speed index (ESI) and expression of superoxide dismutase (SOD), catalase (CAT) and peroxidase (PO) enzymes were assessed. E. erythropappus seed priming in polyethylene glycol solution with potentials between -0.8 MPa and -1.4 MPa is not satisfactory because it adversely affects germination and reduces the activity of peroxidase and catalase enzymes. The other substances were not significant so there is a need for further research with other substances and / or concentrations

scholarly journals Germination and Emergence of Parsley in Response to Osmotic or Matric Seed Priming and Treatment with Gibberellin

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 907-909 ◽  
Author(s):  
Wallace G. Pill ◽  
Elizabeth A. Kilian
Keyword(s):  
Polyethylene Glycol ◽  
Gibberellic Acid ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Petroselinum Crispum ◽  
Hypocotyl Length ◽  
Shoot Dry Weight ◽  
Emergence Percentage

`Moss Curled' seeds of parsley (Petroselinum crispum L.) were primed osmotically in polyethylene glycol or matrically in fine, exfoliated vermiculite at –0.5 MPa for 4 or 7 days at 20 or 30 °C with 0 or 1 mm GA3. All priming treatments stimulated and hastened germination. Matric priming resulted in greater germination (89%) than osmotic priming (83%) when seeds were primed for 7 days at 30 °C, but priming agent had no effect on germination percentage following priming at 20 °C or for 4 days. In seeds primed for 4 days at 20 or 30 °C, matric priming hastened germination more than did osmotic priming. Germination was generally less synchronous with matric than with osmotic priming. Increasing priming time from 4 to 7 days increased the rate of germination, but increased germination synchrony only when seeds were primed at 20 °C. Inclusion of 1 mm GA3 during priming had little or no effect on germination. All matric priming treatments (other than 4-day priming) were repeated to assess seedling emergence in a greenhouse (25°C day/22 °C night). Priming increased the percentage, rate and synchrony of emergence, and increased hypocotyl length at 3 weeks after planting. Priming at 30 °C with 1 mm GA3 resulted in the greatest emergence percentage, hypocotyl length, and shoot dry weight. We conclude that matric priming is a satisfactory alternative to osmotic priming of parsley seeds. Chemical name used: gibberellic acid (GA3).

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