Freeze Tolerance of `Braeburn' Apple Shoots
Differential thermal analysis (DTA) and tetrazolium triphenyl chloride (TTC) were done on shoots of 4-year-old `Braeburn' apple trees for 3 years. The trees acclimated slowly in autumn. If cold temperatures last long enough in winter, shoots will acclimate as low as –40C. Shoots are sensitive to warm temperatures and deacclimated rapidly. An attempt to run a controlled test on freeze resistance of `Braeburn' did not respond to DTA. Moisture samples indicated trees were freeze dried. Different sets of trees were rehydrated and showed an exotherm pattern. Exotherms could be seen after 3 days at 26C, 14 days at 10C, and 21 days at 4C. Another controlled freeze test was performed on 1-year-old `Braeburn' trees. Trees were acclimated outdoors. An exotherm pattern could be seen upon DTA analysis. After artificial freezing, DTA and TTC tests showed pith killed at –24C, primarily xylem at –28C, and all tissue at –35C. After freezing, trees were placed in a greenhouse and warmed over 2 months. Upon dissection, we found xylem produced before freezing was dead, but a large amount of new xylem was generated. Trees appeared to have normal leaf and shoot growth for about a month, but eventually wilted and died. Dissection of these showed the same results as the first set dissected. New xylem evidently was not enough to carry the growth of the trees.