scholarly journals Determination of Optimal Storage Temperature to Maintain Viable Pawpaw [Asimina triloba (L.) Dunal] Seed

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 557A-557 ◽  
Author(s):  
Kirk W. Pomper ◽  
Snake C. Jones ◽  
LaTeasa Barnes
Keyword(s):  
Native American ◽  
Seed Germination ◽  
Storage Temperature ◽  
Germination Rate ◽  
Germplasm Collection ◽  
Peat Moss ◽  
Short Period ◽  
Seed Stratification ◽  
And Storage ◽  
Storage Temperatures

Pawpaw is a native American tree fruit that has great potential as a new commercial crop. The USDA National Clonal Germplasm Repository for Asimina sp. is located at Kentucky State Univ. (KSU); therefore, germplasm collection and storage are important components of the research program. Recalcitrant seeds do not tolerate desiccation, have a relatively short period of viability, and tend not to tolerate subfreezing temperatures. Since pawpaw seed shows a moderate level of recalcitrance, the objectives of this experiment were to determine which storage temperatures (20, 5, -15, and -70 °C for 8.5 weeks) would maintain viable seed, and whether prior seed stratification (5 °C for 17.5 weeks) would influence survival at the various storage temperatures. Seeds were placed in ziplock bags in moist peat moss and subjected to the range of storage temperatures either before or after stratification. After storage and stratification treatments, seed germination rate was examined for 10 weeks at 25 °C on moist filter paper in petri dishes. Both stratification and storage temperature significantly affected seed germination rate. Seeds did not germinate after storage at subfreezing temperatures, regardless of stratification treatment. The best germination rate, ≈70%, was obtained with stratification followed by storage at 20 °C. However, for long-term storage of viable nongerminating pawpaw seed, stratification followed by storage at 5 °C would be most appropriate. Subfreezing storage temperatures were found to be lethal to pawpaw seeds.

scholarly journals Study of storage on seed germination of Chresta sphaerocephala DC. – Asteraceae

2021 ◽  
Vol 10 (9) ◽  
pp. e50110917893
Author(s):  
Amanda Domingas Ediodato de Sousa ◽  
Luciana Botezelli ◽  
Patrícia Neves Mendes
Keyword(s):  
Seed Germination ◽  
Storage Temperature ◽  
Morphological Difference ◽  
Seed Storage ◽  
Seed Vigor ◽  
Significance Level ◽  
Different Temperatures ◽  
And Storage ◽  
Storage Temperatures ◽  
Germinated Seeds

High-altitude fields have been subjected to an intense anthropization process. Due to this, studies on germination, seed storage and propagation of species that compose this plant formation become fundamental. Among such species is the Chresta sphaerocephala DC., Asteraceae. The purpose of this work was to study different temperatures for germination and storage of this species. Four germination temperatures (15°C, 20°C, 25°C and 30°C) and two storage temperatures (-5°C and +5°C) were tested. In order to assess seed vigor, the germination speed index (GSI) and average seed germination time were calculated. ANOVA and Tukey's test were performed at a 5% significance level. In addition, a morphological difference was observed in the analyzed Chresta sphaerocephala seeds, with these being classified as C1 (small seeds), C2 (long thin seeds), C3 (thicker seeds) and C4 (dark colored seeds). As for the storage temperature, 5°C was shown to be the best. When correlated with the germination temperature, 20°C resulted in a statistically higher number of germinated seeds.

Modeling the Effect of Water Activity, pH, and Temperature on the Probability of Enterotoxin A Production by Staphylococcus aureus

2016 ◽  
Vol 79 (1) ◽  
pp. 148-152 ◽  
Author(s):  
TIAN DING ◽  
YAN-YAN YU ◽  
CHENG-AN HWANG ◽  
QING-LI DONG ◽  
SHI-GUO CHEN ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Water Activity ◽  
Storage Temperature ◽  
Probability Model ◽  
Toxin Production ◽  
Factors Affecting ◽  
Model Predictions ◽  
And Storage ◽  
Storage Temperatures ◽  
Good Agreement

ABSTRACT The objectives of this study were to develop a probability model of Staphylococcus aureus enterotoxin A (SEA) production as affected by water activity (aw), pH, and temperature in broth and assess its applicability for milk. The probability of SEA production was assessed in tryptic soy broth using 24 combinations of aw (0.86 to 0.99), pH (5.0 to 7.0), and storage temperature (10 to 30°C). The observed probabilities were fitted with a logistic regression to develop a probability model. The model had a concordant value of 97.5% and concordant index of 0.98, indicating that the model satisfactorily describes the probability of SEA production. The model showed that aw, pH, and temperature were significant factors affecting the probability of toxin production. The model predictions were in good agreement with the observed values obtained from milk. The model may help manufacturers in selecting product pH and aw and storage temperatures to prevent SEA production.

scholarly journals Effect of Ozone and Storage Temperature on Postharvest Diseases and Physiology of Carrots (Daucus carota L.)

1994 ◽  
Vol 119 (3) ◽  
pp. 563-567 ◽  
Author(s):  
Chiam L. Liew ◽  
Robert K. Prange
Keyword(s):  
Ozone Concentration ◽  
Storage Temperature ◽  
Postharvest Diseases ◽  
Total Flow ◽  
Total Flow Rate ◽  
Daily Growth ◽  
Daucus Carota L ◽  
Color Differences ◽  
And Storage ◽  
Storage Temperatures

Effects of ozone and storage temperature on carrots and two postharvest pathogens—Botrytis cinerea Pers. and Sclerotinia sclerotiorum de Bary—were investigated. Pathogen-inoculated and uninoculated whole carrots were exposed to an ozone concentration of 0 (control), 7.5, 15, 30, or 60 μl·liter-1. Treatment chambers were flushed with a total flow rate of 0.5 liters·min-1 (air and ozone) for 8 h daily for 28 days. The experiment was repeated twice at storage temperatures of 2, 8, and 16C. The residual ozone concentration (ozone supplied-exhausted and reacted ozone) increased with ozone supply concentration but was less at higher storage temperatures. A 50% reduction of daily growth rates of both fungi at the highest ozone concentration indicated that ozone was fungistatic. Carrot respiration rate, electrolyte leakage, and total color differences increased with ozone concentration. Ozone-treated carrots were lighter (higher L* values) and less intense (lower chroma values) in color than control carrots.

scholarly journals Effects of gibberellic acid and storage temperature on the germination of hawthorn seeds

2017 ◽  
Vol 63 (No. 9) ◽  
pp. 417-424 ◽  
Author(s):  
Ahmadloo Fatemeh ◽  
Kouchaksaraei Masoud Tabari ◽  
Goodarzi Gholam Reza ◽  
Salehi Azadeh
Keyword(s):  
Electrical Conductivity ◽  
Gibberellic Acid ◽  
Water Uptake ◽  
Temperature Regime ◽  
Storage Temperature ◽  
Fresh Weight ◽  
Freeze Thaw ◽  
Refrigerator Temperature ◽  
And Storage ◽  
Storage Temperatures

This study investigated methods to overcome seed dormancy in Crataegus pseudoheterophylla Pojarkova seeds. Seeds with and without endocarps were treated with gibberellic acid (GA<sub>3</sub>) at different concentrations and four storage temperatures. Then they were stratified in an alternate temperature regime. The amount of absorbed water in seeds with endocarps was monitored by measuring the fresh weight of seeds for 0, 24, 48, 72, and 96 h of imbibition. The electrical conductivity (EC) and the percentage of water uptake by seeds stored for 12 months at laboratory temperature, in a refrigerator, in a freezer, and in freeze-thaw conditions were measured. The highest germination (59.7%) was recorded in seeds without endocarps treated with 3,000 mg·l<sup>–1</sup> GA<sub>3 </sub>and stored either in a laboratory or a refrigerator (32.7–35.3%). All treatments of seeds without endocarps where GA<sub>3</sub> was applied showed statistically higher percentages of germination than the control. Seeds with endocarps stored at refrigerator temperature imbibed water up to 44.3% with increasing imbibition periods, whereas the amount of seeds that absorbed water in freezer and freeze-thaw conditions was almost the same. The tests showed the highest EC during storage in the freezer, with the lowest water uptake and viability in seeds stored during the freeze-thaw process.

scholarly journals GERMINATION OF STORED SEEDS OF FOUR TREE SPECIES FROM THE TROPICAL DRY FOREST OF MORELOS, MEXICO

2014 ◽  
Vol 92 (2) ◽  
pp. 281 ◽  
Author(s):  
Martha Cervantes ◽  
Eliane Ceccon ◽  
Consuelo Bonfil
Keyword(s):  
Seed Germination ◽  
Seed Size ◽  
Storage Time ◽  
Germination Rate ◽  
Tropical Dry Forest ◽  
Dry Forest ◽  
Tropical Dry Forests ◽  
Germination Capacity ◽  
Dry Forests ◽  
And Storage

<p><span style="font-size: medium;"><span style="font-family: Times New Roman;">Studies on propagation of trees of Tropical Dry Forests are scarce in Mexico, besides, the provenance of seeds used in reforestation programs is generally unknown or poorly addressed. Knowledge on seed germination patterns of different provenances, and how they change through time, is useful to identify adequate sources of seeds and to develop seed collection and storage programs under the low-tech conditions prevailing in most rural nurseries. We evaluated seed size variation and germination of stored seeds from three different provenances per species in <em>Acacia bilimekii</em>, <em>Haematoxylum brasiletto</em>, <em>Lysiloma acapulcense</em>, and<em> L. divaricatum</em>.<em> </em>Seeds were collected in four sites in the Tropical Dry Forest of Morelos, Mexico, and were stored at room temperature; seed size was estimated through the volume of 75 seeds per provenance/species. Seed germination tests were made periodically from six to 24 months after storage and the effects of provenance and storage time on germination were analyzed using Anovas. There were significant differences in seed size among provenances in all species, while the effect of provenance on germination rate was significant in three of them. Germination rate changed with storage time among species and provenances. After 24 months, germination capacity was still ≥ 50% in all provenances of the two <em>Lysiloma</em> species, but in <em>A.</em> <em>bilimekii</em> there were large differences among provenances. Mean germination capacity was low in <em>H. brasiletto</em> after six months. More research on seed germination and storage of a larger set of species and provenances is needed to restore the Mexican tropical dry forests. </span></span></p>

scholarly journals Antioxidizing Enzyme Activities in Chilling-sensitive and Chilling-tolerant Pepper Fruit as Affected by Stage of Ripeness and Storage Temperature

2009 ◽  
Vol 134 (1) ◽  
pp. 156-163 ◽  
Author(s):  
Chae Shin Lim ◽  
Seong Mo Kang ◽  
Jeoung Lai Cho ◽  
Kenneth C. Gross
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Ethylene Production ◽  
Electrolyte Leakage ◽  
Storage Temperature ◽  
Chilling Injury ◽  
Pepper Fruit ◽  
And Storage ◽  
Storage Temperatures ◽  
Β Carotene

To study ripening-related chilling injury in pepper (Capsicum annuum L.), chilling-tolerant ‘Buchon’ and chilling-sensitive ‘Nockgwang’ fruit were harvested at mature green (MG), breaker (BR), and red-ripe (RR) stages and stored at 1, 5, and 10 °C for 21 d. ‘Buchon’ did not show surface pitting (SP) regardless of ripeness stage and storage temperature, whereas ‘Nockgwang’ at MG and BR exhibited SP at 1 and 5 °C. After 14 days of storage at 1 °C, chilling-sensitive ‘Nockgwang’ did not show SP when fruit were at the RR stage. Compared with ‘Buchon’, ‘Nockgwang’ at MG and BR had more electrolyte leakage increase during storage at 1 and 5 °C. ‘Buchon’ at all ripeness stages showed significantly higher ethylene production during storage regardless of storage temperatures. Contents of β-carotene and lycopene increased in both cultivars as ripening progressed. The contents of β-carotene and lycopene were similar between the two cultivars regardless of storage temperatures and ripeness stages. Susceptibility of pepper fruit to chilling appeared to be related to superoxide dismutase (SOD) and catalase (CAT). Activities of SOD and CAT were much higher in ‘Buchon’ than ‘Nockgwang’, more apparently at MG and BR. The results suggest that chilling-tolerant ‘Buchon’ and fruit at RR could have been equipped with a more efficient antioxidizing system, even if it was not clear whether oxidative stress is a cause or an effect of the CI in pepper.

scholarly journals Textural Changes of Asparagus in Relation to Delays in Cooling and Storage Conditions

1992 ◽  
Vol 2 (3) ◽  
pp. 378-381 ◽  
Author(s):  
Luis Hernández-Rivera ◽  
Robert Mullen ◽  
Marita Cantwell
Keyword(s):  
Shear Force ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Asparagus Officinalis ◽  
Shear Transfer ◽  
Textural Changes ◽  
And Storage ◽  
Temperature Time ◽  
Storage Temperatures

Asparagus (Asparagus officinalis L.) spears (cv. UC 157) trimmed to 20 cm were cooled 0, 4, 8, 12, 16, and 20 hours after harvest and evaluated for resistance to shear at 5 and 10 cm above the cut end immediately after cooling and during storage at 0.5, 2.5, and 5C. Resistance to shear increased during cooling delays and with time in storage. Initial cooling delays and simulated marketing conditions (transfer to 15C for 1 day) were the principal causes of increased resistance to shear. A 4-hour cooling delay resulted in an average 40% increase in resistance to shear. Transfer to 15C for 1 day resulted in a greater increase in shear force in the rapidly cooled than in the delayed-cooled spears. Increases in resistance to shear during storage varied from 0% to 50% and depended on the storage temperature, time, and the initial cooling delay. Spears trimmed to a green base showed less increase in shear force after storage than did spears trimmed to a white base. The visual quality of asparagus stored for 14 days was similar (excellent) among spears from different storage temperatures and between green- and white-base spears. Storage quality after 24 days had decreased more in spears stored at 5C than at 2.5 or 0.5C, and more in the green-base than the white-base spears.

scholarly journals Irradiation, Stage of Maturity at Harvest, and Storage Temperature during Ripening Affect Papaya Fruit Quality

HortScience ◽  
1999 ◽  
Vol 34 (6) ◽  
pp. 1112-1115 ◽  
Author(s):  
W.R. Miller ◽  
R.E. McDonald
Keyword(s):  
Carica Papaya ◽  
Storage Temperature ◽  
Cold Temperatures ◽  
Green Fruit ◽  
Fruit Maturity ◽  
Before And After ◽  
Ripe Stage ◽  
And Storage ◽  
Degree Of Severity ◽  
Storage Temperatures

Solo-type papaya (Carica papaya L.) fruit at the mature green (MG) or one-quarter yellow (QY) stage of maturity were imported through the Port of Miami, Fla., and either irradiated (0.675 kGy) or not irradiated. Fruit condition and quality attributes were determined after ripening to the edible ripe stage at 25 °C before and after storage for 7 days at 10, 12, or 15 °C. The incidence and severity of peel scald was increased by irradiation regardless of storage and ripening regime; however, the degree of severity was dependent on fruit maturity at irradiation. Irradiated QY fruit tended to have the most serious incidence and severity of scald. Mature green fruit ripened at 25 °C without storage had the lowest incidence of fruit with hard areas in the pulp (“lumpy” fruit). The QY fruit generally were second only to irradiated MG fruit stored at 10 °C in incidence of lumpiness. Anthracnose sp. decay and stem-end-rots affected 53% of all fruit. The least decay occurred on fruit ripened at 25 °C without storage, regardless of fruit maturity, and the most decay occurred on QY fruit with or without irradiation. Fruit ripened at 25 °C without storage had more palatable pulp (5.5 N) at the edible ripe stage than did fruit held in storage and then ripened. The effect of fruit maturity or irradiation dose on fruit firmness, however, was dependent on the storage temperature. Mature green fruit ripened at 25 °C lost less weight than did those stored at cold temperatures prior to ripening. We recommend that importers obtain fruit with only a slight break in ground color, and distribute them as rapidly as possible, while maintaining transit/storage temperatures at or above 15 °C with or without exposure to irradiation.

scholarly journals Pengaruh Aminoethoxyvinylglycine (Avg), Plastic Wrapping Dan Suhu Simpan Terhadap Masa Simpan Dan Mutu Buah Jambu Biji (Psidium Guajava L.) ‘Mutiara’

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Soesiladi E. Widodo ◽  
Kuswanta F. Hidayat ◽  
Zulferiyenni Zulferiyenni ◽  
Sherly I. Annisa
Keyword(s):  
Weight Loss ◽  
Storage Temperature ◽  
Psidium Guajava ◽  
Interaction Effects ◽  
Fruit Weight ◽  
Fruit Firmness ◽  
Single Application ◽  
The Third ◽  
And Storage ◽  
Storage Temperatures

The research was aimmed at studying the effects of (1) single application AVGs,plastic wrappings, and storage temperatures, (2) interactions among AVGs andplastic wrappings, AVGs and storage temperatures, and plastic wrappings andstorage temperatures, and (3) interactions among AVGs, plastic wrappings, andstorage temperatures in extending fruit self-life and maintain qualities of‘Mutiara’guava fruits. The research was arranged in a completely randomized designin 2 x 2 x 2 factorial with three replications. The first factor was AVGs (with 1,25ppm and without AVG), the second factor was plastic wrappings (without and withone layer of plastic wrapping), and the third factor was storage temperature (a coldtemperature of 21, 53 ºC and a room temprature of 26,57 ºC). The result showed that(1) the application of AVG 1,25 ppm did not significantly affect fruit self-life, fruitfirmness, fruit weight loss, ºBrix, acid contens, and sweetness of ‘Mutiara’guava’fruits, (2) storage at cold temprature of 21, 53 ºC did not significantly affect fruit self-life, fruit firmness, fruit weight loss, ºBrix, acid contens, and sweetness of‘Mutiara’guava’ fruits, (3) the application of plastic wrapping was able to extend fruitself-life by 17 days longer than without plastic wrapping and supressed fruit weightloss of ‘Mutiara’guava, but accelerated softening and not able to maintain ºBrix, andalso did not significantly affect acid contens, and sweetness of ‘Mutiara’guava fruits,(4) there were no interaction effects among AVGs with plastic wrappings, AVGs withcold tempratures, and plastic wrappings with cold tempratures on self-life, fruitfirmness, fruit weight loss, ºBrix, acid contens, and sweetness of ‘Mutiara’guavafruits, and (5) there were no interaction effects among AVGs, plastic wrappings, andcold temperatures on fruit self-life, fruit firmness, fruit weight loss, ºBrix, acidcontens, and sweetness of ‘Mutiara’ guava fruits.

scholarly journals PENGARUH WADAH, SUHU DAN WAKTU SIMPAN TERHADAP PERKECAMBAHAN BENIH MENIRAN (Phyllanthus niruri L.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Nurul Husniyati Listyana ◽  
Rahma Widyastuti ◽  
Widyantoro Widyantoro
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Storage Temperature ◽  
Storage Period ◽  
Raw Material ◽  
Wild Plant ◽  
Phyllanthus Niruri ◽  
Storage Container ◽  
Genetic Traits ◽  
And Storage

ABSTRACT Meniran (Phyllanthus niruri L.) is a wild plant that used extensively as a raw material for traditional medicine but has not been cultivated yet. Meniran cultivation requires the availability of quality seeds and seedlings to produce the high productivity. The ability of seeds to germinate (seed viability) after the storage period is influenced by several factors both internal and external, including seed water content, genetic traits, initial viability and packaging material, storage temperature and humidity, microorganisms, and human factors.. The study aims to determine the effect of storage containers, storage temperature and time of storage on the power of meniran seed germination. The study was conducted in March-April 2018 at the B2P2TO2T Seed Laboratory. The research design was Factorial Completely Randomized Design with 3 factors and 3 replications. The first factor was a storage container consisting of plastic, paper envelopes and aluminum foil. Second factor was a storage temperature consisting of 0 ° C, -20 ° C and -50 ° C.  The third factor was storage time which consists of 2 weeks, 4 weeks, 6 weeks, 8 weeks and 10 weeks. The results showed that the storage container and storage time had a significantly different effect on meniran seed germination. The storage time does not have a significant effect on germination. Together, the storage container, storage temperature and storage time do not have a significant effect on meniran seed germination. The interaction between the storage temperature and the storage time and the interaction between the storage container and the storage time give significantly different effects on germination. ABSTRAK Meniran (Phyllanthus niruri L.) merupakan tanaman liar yang simplisianya banyak dibutuhkan sebagai bahan baku obat tradisional namun belum dibudidayakan. Budidaya tanaman meniran memerlukan ketersediaan benih dan bibit yang bermutu untuk menghasilkan produktivitas yang tinggi. Kemampuan benih untuk berkecambah (viabilitas benih) setelah masa penyimpanan dipengaruhi oleh beberapa faktor baik internal maupun eksternal, meliputi kadar air benih, sifat genetik, viabilitas awal serta bahan pengemas, suhu dan kelembaban ruang simpan, mikroorganisme, serta faktor manusia. Penelitian bertujuan untuk mengetahui pengaruh wadah simpan, suhu simpan dan waktu simpan terhadap daya perkecambahan benih meniran. Penelitian dilaksanakan pada bulan Maret-April 2018 di Laboratorium Benih B2P2TO2T. Desain penelitian yang digunakan yaitu Rancangan Acak Lengkap Faktorial dengan 3 faktor dan 3 ulangan. Faktor 1 yaitu wadah penyimpanan yang terdiri dari plastik, kertas amplop dan aluminium foil. Faktor 2 yaitu suhu simpan yang terdiri dari 0°C, -20°C dan -50°C. Faktor 3 yaitu waktu penyimpanan yang terdiri dari 2 minggu, 4 minggu, 6 minggu, 8 minggu dan 10 minggu. Hasil penelitian menunjukkan wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran.  Suhu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan. Secara bersama-sama wadah simpan, suhu simpan dan waktu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran. Interaksi antara suhu simpan dan waktu simpan serta interaksi antara wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan.  

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