scholarly journals Arabica coffee accessions originated from Ethiopia with resistance to nematode Meloidogyne paranaensis

2020 ◽  
pp. 1209-1213
Author(s):  
Melina Marques Holderbaum ◽  
Dhalton Shiguer Ito ◽  
Débora Cristina Santiago ◽  
Luciana Harumi Shigueoka ◽  
Lucas Eduardo Fernandes ◽  
...  
Keyword(s):  
Coffea Arabica ◽  
Germplasm Bank ◽  
Heterozygous Condition ◽  
Arabica Coffee ◽  
Final Population ◽  
Randomized Design ◽  
Susceptible Control ◽  
Completely Randomized Design ◽  
Reproduction Factor

The aim of this study was to evaluate the resistance of Ethiopian Coffea arabica from IAPAR’s germplasm bank to Meloidogyne paranaensis. Two experiments were carried out in a greenhouse in IAPAR, Londrina, Paraná State, Brazil. A completely randomized design was used with 15 treatments, eight replications and one plant per plot. 14 accessions of C. arabica from Ethiopia were evaluated and the cultivar Mundo Novo IAC 376-4 was used as a susceptible control. Seedlings with three to four pairs of leaves were transplanted into plastic cups with a capacity of 700 mL and 1200 eggs and/or J2 of M. paranaensis (IP) were inoculated after one month. The evaluations were performed 130 days after inoculation, when the data of the number of eggs and J2 per gram of roots, the final population of nematodes (FP) were obtained and the reproduction factor (RF) was calculated using the formula: RF = IP / FP. To classify the resistance levels of the genotypes, the reduction of the reproduction factor (RRF) was used. The Ethiopian accessions were classified from highly resistant to highly susceptible. Most of the Ethiopian coffees were highly resistant to M. paranaensis. The genotype Geisha also showed resistance, but is still in heterozygous condition.

scholarly journals Resistance to nematode Meloidogyne paranaensis in Arabica coffee genotypes introgressed with Coffea liberica

2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Mother Plants ◽  
Completely Randomized Design ◽  
Set Up ◽  
Moderately Resistant ◽  
Reproduction Factor ◽  
Coffea Liberica

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.

scholarly journals Intermediate resistance to nematode Meloidogyne paranaensis in Híbrido de Timor coffee genotypes

2021 ◽  
pp. 628-633
Author(s):  
Gustavo Hiroshi Sera ◽  
Lucas Eduardo Fernandes ◽  
Inês Cristina de Batista Fonseca ◽  
Dhalton Shiguer Ito ◽  
Luciana Harumi Shigueoka ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Resistance Sources ◽  
Final Population ◽  
Randomized Design ◽  
Intermediate Resistance ◽  
Completely Randomized Design ◽  
Moderate Resistance ◽  
Different Levels ◽  
Reproduction Factor

The aim of this study was to prove that Híbrido de Timor (HdT) accessions are resistance sources to Meloidogyne paranaensis. Two experiments were carried out in a greenhouse at IDR-Paraná (Londrina, Paraná State, Brazil). Open pollinated fruits were harvested from 10 accessions (HdT UFV 380-05, HdT UFV 408-10, HdT UFV 408-11, HdT UFV 408-28, HdT UFV 428-04, HdT UFV 439-14, HdT UFV 443-08, HdT UFV 445-70, HdT UFV 446-138, HdT UFV 448-75) from the EPAMIG/ UFV germplasm bank. Seeds of these accessions were sown to obtain seedlings to test the resistance to M. paranaensis. For each experiment, a completely randomized design was used with 11 treatments, eight replications and one plant per plot. Cultivar Mundo Novo IAC 376-4 was the susceptible check. Seedlings with three to four pairs of leaves were transplanted into plastic cups with a capacity of 700 mL and 1200 eggs. The J2 of M. paranaensis (IP) were inoculated after one month. The assessments were performed 134 days after inoculation, when the data of the number of eggs and J2 per gram of roots and the final population of nematodes (FP) were obtained. The reproduction factor (RF) was calculated using the formula: RF = IP / FP. To classify the resistance levels of the genotypes, the reproduction factor reduction (RFR) was used. It classified the levels from highly resistant to highly susceptible. Different levels of intermediate resistance between the accesses were observed, highlighting HdT UFV 408-28 that presented moderate resistance to M. paranaensis. HdT UFV 408-28 showed a high percentage of plants with intermediate resistance, which can be used in breeding programs aimed at resistance to M. paranaensis

scholarly journals Resistance to red mite in Coffea arabica genotype introgressed with Coffea racemosa genes

2019 ◽  
pp. 683-686
Author(s):  
Fernando Cesar Carducci ◽  
Inês Cristina de Batista Fonseca ◽  
Willian Gabriel dos Santos ◽  
Carlos Theodoro Motta Pereira ◽  
Valdir Mariucci Junior ◽  
...  
Keyword(s):  
Leaf Area ◽  
Coffea Arabica ◽  
Visual Evaluation ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Susceptible Control ◽  
Natural Infestation ◽  
Completely Randomized Design

The aim of this study was to assess the resistance to red mite (Oligonychus ilicis) in Coffea arabica genotypes with introgression of genes from a different species (Coffea racemosa). The experiment was conducted in a greenhouse at IAPAR (Londrina, PR, Brazil), between January 2016 and January 2017. The experiment was conducted in a completely randomized design with five genotypes, 12 replications of one plant per plot. The C. arabica cultivars IPR 100, IPR 99 and IPR 103 were evaluated as well as an F4 Arabica coffee line IAPAR H0113-40-26-10 with introgressed C. racemosa genes. Catuaí Vermelho IAC 99 cultivar, which is a pure C. arabica, was used as a susceptible control. In conditions of high natural infestation of red mite, the resistance of coffee genotypes was evaluated using a standard visual evaluation in January 2017. This evaluation was based on the percentage of leaf area with typical symptoms caused by this mite. Only one genotype, the F4 line IAPAR H0113-40-26-10 showed resistance to the red mite because present less leaf area with symptoms compared to other genotypes. The Arabica coffee cultivars IPR 99, IPR 100 and IPR 103 are as susceptible to red mite as to the susceptible control Catuaí Vermelho IAC 99.

scholarly journals Effect of Vermicompost Application to Arabica Coffee Seedlings (Coffea arabica L.)

2020 ◽  
pp. 1-9
Author(s):  
Indra Dwipa ◽  
Merlyana Boangmanalu ◽  
Musliar Kasim ◽  
Dedi Azwardi
Keyword(s):  
Sea Level ◽  
Seedling Growth ◽  
Coffea Arabica ◽  
Experimental Unit ◽  
Arabica Coffee ◽  
Multiple Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Coffea Arabica L ◽  
Experimental Garden

Aims: The research aimed to study the effect of several dose of vermicompost to arabica coffee seedling growth. Study Design: Completely randomized design. Place and Duration of Study: The research was conducted in experimental garden of Faculty of Agriculture, Andalas University from September to December 2018. The altitude of research site was 385 meter above sea level (asl). Methodology: The research aimed to study the effect of several dose of vermicompost to arabica coffee seedling growth. Completely randomized design was used in the research that consisted of 5 treatments (No vermicompost, 5 ton/ha, 10 ton/ha, 15 ton/ha and 20 ton/ha) and replied 3 times. Each experimental unit consisted of 6 seedlings. The data was analyzed by Duncan’s New Multiple Test in 5%. Results: The result showed that 5 ton/ha of vermicompost dose has affected the growth component of arabica coffee seedling. Conclusion: The addition of vermicompost dose affected the arabica coffee seedlings growth.

scholarly journals Reaction of melon genotypes to Meloidogyne incognita and Meloidogyne javanica

2016 ◽  
Vol 46 (1) ◽  
pp. 111-115
Author(s):  
Guilherme Matos Martins Diniz ◽  
Willame dos Santos Candido ◽  
Renato Silva Soares ◽  
Lucas da Silva Santos ◽  
Marcus Vinícius Marín ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Santa Cruz ◽  
Breeding Programs ◽  
Northeast Region ◽  
Resistant Cultivars ◽  
Randomized Design ◽  
Susceptible Control ◽  
Completely Randomized Design ◽  
Reproduction Factor

ABSTRACT Melon is one of the most economically important crops cultivated in Brazil, especially in the Northeast region. However, as its cultivation increases, phytosanitary problems arise, including those caused by nematodes, which are most effectively controlled using resistant cultivars. This study aimed at assessing the reaction of muskmelon genotypes, in terms of resistance to Meloidogyne incognita and M. javanica infestation. The experiment was conducted under greenhouse conditions using a completely randomized design, in a 2 x 15 factorial scheme, with six replications. A total of 15 muskmelon genotypes were evaluated and the 'Santa Cruz Kada' tomato was used as a susceptible control. The total number of eggs and juvenile nematodes in the roots and the reproduction factor were used to assess the genotype reaction. None of the genotypes was resistant to M. incognita. Eight genotypes were classified as resistant to M. javanica and promising for use in melon breeding programs.

scholarly journals Influence of Meloidogyne incognita race 1 on the development of clones of Coffea canephora, variety “Jequitibá Incaper 8122”

2020 ◽  
Vol 87 ◽  
Author(s):  
Lilian Katiany Castello Rabello Zinger ◽  
Fernando Domingo Zinger ◽  
Fábio Ramos Alves ◽  
Waldir Cintra de Jesus Junior ◽  
Angelo Oliveira Gonçalves ◽  
...  
Keyword(s):  
Coffea Canephora ◽  
Root Knot Nematode ◽  
Fresh Matter ◽  
Second Stage ◽  
Final Population ◽  
Randomized Design ◽  
Race 1 ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Reproduction Factor

ABSTRACT: Root-knot nematode is one of the most important phytosanitary problems for Conilon coffee, as it reduces productivity and is difficult to handle. We aimed at studying the infectivity and damage caused by M. incognita race 1 in the “Jequitibá Incaper 8122” intermediate maturity coffee variety. The experiment was conducted in a greenhouse, in completely randomized design, with five replicates. The clones composing the variety “Jequitibá Incaper 8122” were inoculated with 2,000 eggs + second-stage juveniles of M. incognita race 1. Uninoculated plants were the control. Evaluations were performed 180 days after inoculation, considering the plant height (H), stem diameter (SD), number of leaves (NOL), leaf area (LA), number of plagiotropic branches (NPB), number of nodes (NN), chlorophyll content (CHLO), shoot dry matter (SDM), root fresh matter (RFM), final population (FNP), and reproduction factor (NRF). The nematode reduced NOL in clones 208 and 209, NRF in clones 201, 203, 207 and 208, NN in clones 203, 207, 208 and 209, CHLO in clones 201, 204, 206, 207 and 209, SDM in clones 201, 203, 204 and 205 and RFM in clones 205 and 207. M. incognita race 1 FNP and NRF were larger in clones 208, 201, 207 and 203. Clone 202 had FNP and NRF equal to zero, being immune to the nematode. Clone 206 presented the lowest NRF value among clones parasitized by M. incognita.

scholarly journals Seed treatment for management of Meloidogyne javanica in soybean

2017 ◽  
Vol 38 (5) ◽  
pp. 2995 ◽  
Author(s):  
Adriely Alves de Almeida ◽  
Vinicius Hicaro Frederico Abe ◽  
Ricardo Marcelo Gonçalves ◽  
Maria Isabel Balbi-Peña ◽  
Débora Cristina Santiago
Keyword(s):  
Seed Treatment ◽  
Meloidogyne Javanica ◽  
Vitro Assay ◽  
Juvenile Mortality ◽  
Second Stage ◽  
Final Population ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Soybean Plants

The objective of this study was to evaluate the efficacy of the active ingredient abamectin, either in isolation or in combination with fungicides and insecticides formulated for the industrial seed treatment, on the population of Meloidogyne javanica, tested under greenhouse and in vitro conditions. In both tests, the combination of the following treatments was assessed: abamectin; thiamethoxam; fludioxonil + metalaxyl-M + thiabendazole. Water was used as control for the in vitro assay, whereas under greenhouse conditions, controls were inoculated and not inoculated with M. javanica. The tests were conducted in a completely randomized design with six (in vitro) and ten (greenhouse) replicates. For in vitro studies the effect on hatching, motility, and mortality of juveniles of M. javanica was evaluated. Under greenhouse conditions, the soybean ‘BRS 133’ seeds were treated, and at 15, 30, and 60 days after inoculation (DAI) with M. javanica, plant measurements were recorded. The penetration of second stage juveniles (J2) was also evaluated at 15 DAI. At 30 DAI, galls, egg masses, nematodes/g of root, and final population were evaluated. At 60 DAI, the final population of nematodes in the roots was quantified. The treatments containing abamectin were the most effective in diminishing the hatching of juveniles. All treatments had an effect on nematode motility when compared to the control, and in the treatment containing only abamectin, total juvenile mortality was observed. In greenhouse conditions, at 15 DAI, the treated soybean plants had the highest root mass and shoot length, differing statistically from the inoculated control. All treatments reduced the number of nematodes per gram of root, differing from the control. At 30 DAI, treatment efficiency was observed in reducing the final population of M. javanica, particularly the treatments using abamectin, and abamectin + thiamethoxam + fludioxonil + metalaxyl-M + thiabendazole. However, at 60 DAI, the effect of the treatments on the population of M. javanica did not persist.

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

scholarly journals Do the stage of plant and initial population of root knot nematode influence on sweet pepper development?

O Biológico ◽  
2020 ◽  
Vol 82 (1) ◽  
pp. 1-10
Author(s):  
Samara A. de Oliveira ◽  
Juliana M. O Rosa ◽  
Juliana Eulálio ◽  
Claudio Marcelo G. de Oliveira
Keyword(s):  
Sweet Pepper ◽  
Initial Population ◽  
Root Knot Nematode ◽  
One Pot ◽  
Leaf Pair ◽  
Final Population ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Shoot Weight ◽  
Reproduction Factor

The aim of this study was to investigate the response of three different stages (one, three and five leaf pairs) of sweet pepper (Capsicum annuum cv. Orazio) seedlings to five inoculation levels of Meloidogyne enterolobii (zero (control), 300, 1000, 3000 and 10000) under greenhouse conditions. Each plant was cultivated in one pot filled with 3.8 L of substrate. The test was a completely randomized design with four replications. The plants were assessed 60 days after inoculation, plant shoot weight, final population of nematodes, and reproduction factor were measured. The results were fitted to Seinhorst model: Y = m + (1-m). ZPi –T. The results showed a tolerance limit (T) of 2,500 nematodes for plants with one and three leaf pair, and 8,500 nematodes for the five-leaf pair plant.

scholarly journals RESISTANCE OF POTATO GENOTYPES TO Meloidogyne javanica

2017 ◽  
Vol 30 (2) ◽  
pp. 536-540 ◽  
Author(s):  
JAQUELINE TAVARES SCHAFER ◽  
CESAR BAUER GOMES ◽  
ARIONE DA SILVA PEREIRA ◽  
FERNANDA FERREIRA CRUZ ◽  
DANIELLE RIBEIRO DE BARROS
Keyword(s):  
Potato Cultivar ◽  
Meloidogyne Javanica ◽  
Temperate Climate ◽  
Initial Population ◽  
Nematode Reproduction ◽  
Potato Plants ◽  
Final Population ◽  
Susceptible Control ◽  
Different Levels ◽  
Reproduction Factor

ABSTRACT The objective of this study was to evaluate the resistance of a group of clones and cultivars from the potato breeding program of Embrapa to Meloidogyne javanica. The experiment was conducted at Embrapa Temperate Climate, Pelotas / RS, under greenhouse conditions at temperatures of 25 ± 5°C. Individual potato plants of different genotypes [BRSIPR Bel, BRS F63 (Camila), CL02-05, F23-11-06, F32-02-06, F38-03-07, F189-09-06, F23-24-06 and F22-01-08], kept in pots with sterilized soil were inoculated with 5,000 eggs and J2 of M. javanica, using six replicates per treatment. The susceptible control, potato cultivar BRS Ana was inoculated with the same level of inoculum. Fifty-five days after inoculation, the number of galls on the roots of each plant was determined as well as the number of protuberances caused by nematodes in the different treatments. Then, the roots of each plant were processed for counting the number of eggs and J2, as well as determining the nematode reproduction factor (FR: final population / initial population). All genotypes were susceptible (FR> 1.00) to M. javanica. However, there were different levels of susceptibility among the cultivars tested.

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