scholarly journals Effect of Vermicompost Application to Arabica Coffee Seedlings (Coffea arabica L.)

2020 ◽  
pp. 1-9
Author(s):  
Indra Dwipa ◽  
Merlyana Boangmanalu ◽  
Musliar Kasim ◽  
Dedi Azwardi
Keyword(s):  
Sea Level ◽  
Seedling Growth ◽  
Coffea Arabica ◽  
Experimental Unit ◽  
Arabica Coffee ◽  
Multiple Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Coffea Arabica L ◽  
Experimental Garden

Aims: The research aimed to study the effect of several dose of vermicompost to arabica coffee seedling growth. Study Design: Completely randomized design. Place and Duration of Study: The research was conducted in experimental garden of Faculty of Agriculture, Andalas University from September to December 2018. The altitude of research site was 385 meter above sea level (asl). Methodology: The research aimed to study the effect of several dose of vermicompost to arabica coffee seedling growth. Completely randomized design was used in the research that consisted of 5 treatments (No vermicompost, 5 ton/ha, 10 ton/ha, 15 ton/ha and 20 ton/ha) and replied 3 times. Each experimental unit consisted of 6 seedlings. The data was analyzed by Duncan’s New Multiple Test in 5%. Results: The result showed that 5 ton/ha of vermicompost dose has affected the growth component of arabica coffee seedling. Conclusion: The addition of vermicompost dose affected the arabica coffee seedlings growth.

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

scholarly journals Population of Pratylenchus coffeae (Z.) and growth of Arabica coffee seedling inoculated by Pseudomonas diminuta L. and Bacillus subtilis (C.).

2015 ◽  
Vol 31 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Irfan Fauzi ◽  
Soekadar Wiryadiputra
Keyword(s):  
Bacillus Subtilis ◽  
Seedling Growth ◽  
Coffea Arabica ◽  
Additional Treatment ◽  
Density Level ◽  
Arabica Coffee ◽  
Pratylenchus Coffeae ◽  
Pseudomonas Diminuta ◽  
The Moment ◽  
Coffea Arabica L

AbstractPratylenchus coffeae is a parasitic nematoda that infected the roots of some plants, one of them is coffee. The Infection of Pratylenchus coffeae cause root tissue damage that led to root lession and make root become rotten, it will interfere the ability of roots to absorb water and nutrients in the soil which resulted in the growth of plants. At the moment, control of Pratylenchus coffeae are following integrated pests management (IPM) program, which integrated between the use of coffee resistant clone and application of biological agents. Research on biological control was conducted more intensive, at the moment; due to it is friendlier save against environment and cheaper then using chemical nematicides. The research was conducted to know the effects of Micorrhiza Helper Bacteria (MHB),Pseudomonas diminuta and Bacillus subtilis in suppressing the population of P. coffeaeas well as their effect on growth of coffee seedling.  Coffee arabica (Coffea arabica L.) seedling one moth old were used in the experiment. The experiment prepared with eight treatments and five  replications, as follows: A (Pseudomonas diminuta with density of 108 cfu / ml), B (Pseudomonas diminuta with density of 2x108 cfu / ml), C (Bacillus subtilis with density of 108 cfu / ml ), D (Bacillus subtilis with density 2x108 cfu / ml), E (Carbofuran nematicide 5 g formulation / pot), F (Pseudomonas diminuta and Bacillus subtilis with each density of 108 cfu / ml), K- (Nematoda inoculation but without bacteria and nematicide), K+ (coffee seedling  without any additional treatment). The experiment was conducted for sixteen weeks or about four months. The results of the experiment showed that application of MHB could suppress population of P. coffeae and increase coffee seedling growth significantly. Inoculation of B. subtilis at 108 cfu per seedling suppressed significantly nematoda population of 71.3% compared with untreated seedling but inoculated with nematoda. It was not significant different with carbofuran treatment which could suppress nematoda population by 89.7%. The same result also occur on the treatment of P. diminuta at density level 2.108 cfu/seedling, which could suppress nematoda population by 64.2%. Seedling growth were treated with MHB also significantly increase compared with seedling without treatment and inoculation of nematodas, especially on the treatment of B.subtilis at density level 108 cfu and P.diminuta at density level of 108 cfu, with increasing level of 35.4% and 34.2 %, respectively.Keywords: MHB (Mycorrhiza Helper Bacteria), Arabica coffee (Coffea arabica L.),  Pratylenchus coffeae, Bacillus subtilis, Pseudomonas diminuta.

scholarly journals Arabica coffee accessions originated from Ethiopia with resistance to nematode Meloidogyne paranaensis

2020 ◽  
pp. 1209-1213
Author(s):  
Melina Marques Holderbaum ◽  
Dhalton Shiguer Ito ◽  
Débora Cristina Santiago ◽  
Luciana Harumi Shigueoka ◽  
Lucas Eduardo Fernandes ◽  
...  
Keyword(s):  
Coffea Arabica ◽  
Germplasm Bank ◽  
Heterozygous Condition ◽  
Arabica Coffee ◽  
Final Population ◽  
Randomized Design ◽  
Susceptible Control ◽  
Completely Randomized Design ◽  
Reproduction Factor

The aim of this study was to evaluate the resistance of Ethiopian Coffea arabica from IAPAR’s germplasm bank to Meloidogyne paranaensis. Two experiments were carried out in a greenhouse in IAPAR, Londrina, Paraná State, Brazil. A completely randomized design was used with 15 treatments, eight replications and one plant per plot. 14 accessions of C. arabica from Ethiopia were evaluated and the cultivar Mundo Novo IAC 376-4 was used as a susceptible control. Seedlings with three to four pairs of leaves were transplanted into plastic cups with a capacity of 700 mL and 1200 eggs and/or J2 of M. paranaensis (IP) were inoculated after one month. The evaluations were performed 130 days after inoculation, when the data of the number of eggs and J2 per gram of roots, the final population of nematodes (FP) were obtained and the reproduction factor (RF) was calculated using the formula: RF = IP / FP. To classify the resistance levels of the genotypes, the reduction of the reproduction factor (RRF) was used. The Ethiopian accessions were classified from highly resistant to highly susceptible. Most of the Ethiopian coffees were highly resistant to M. paranaensis. The genotype Geisha also showed resistance, but is still in heterozygous condition.

scholarly journals PENGARUH MEDIA TANAM TERHADAP PERKECAMBAHAN BENIH KOPI ARABIKA (Coffea arabica L)

2020 ◽  
Vol 4 (2) ◽  
pp. 64
Author(s):  
Yana Taryana ◽  
Lia Sugiarti
Keyword(s):  
Seed Germination ◽  
Coffea Arabica ◽  
Block Design ◽  
Mixed Media ◽  
Arabica Coffee ◽  
Randomized Block Design ◽  
The Media ◽  
Coffea Arabica L ◽  
Coffee Seed ◽  
Experimental Garden

A study to determine the effect of various types of mixed planting media on Arabica coffee seed germination was carried out in April to May 2019, located in the Experimental Garden of the Faculty of Agriculture, University of Winaya Mukti, Tanjungsari, Sumedang. This study used a randomized block design (RBD) consisting of 4 treatments and 5 replications. The planting media used in this study were mixed media consisting of A = soil; B = mixture of soil and manure (1: 1); C = mixture of soil and charcoal (1: 1); D = mixture of soil, manure and husk charcoal (1: 1: 1), The results of the study showed the planting media in the form of soil, a mixture of soil with manure or charcoal husk, and the mixture of the three did not affect the germination of seeds, but the use of in the form of a mixture of soil with manure and husk charcoal can increase the germination index compared to the media in the form of soil without mixture.

scholarly journals Resistance to red mite in Coffea arabica genotype introgressed with Coffea racemosa genes

2019 ◽  
pp. 683-686
Author(s):  
Fernando Cesar Carducci ◽  
Inês Cristina de Batista Fonseca ◽  
Willian Gabriel dos Santos ◽  
Carlos Theodoro Motta Pereira ◽  
Valdir Mariucci Junior ◽  
...  
Keyword(s):  
Leaf Area ◽  
Coffea Arabica ◽  
Visual Evaluation ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Susceptible Control ◽  
Natural Infestation ◽  
Completely Randomized Design

The aim of this study was to assess the resistance to red mite (Oligonychus ilicis) in Coffea arabica genotypes with introgression of genes from a different species (Coffea racemosa). The experiment was conducted in a greenhouse at IAPAR (Londrina, PR, Brazil), between January 2016 and January 2017. The experiment was conducted in a completely randomized design with five genotypes, 12 replications of one plant per plot. The C. arabica cultivars IPR 100, IPR 99 and IPR 103 were evaluated as well as an F4 Arabica coffee line IAPAR H0113-40-26-10 with introgressed C. racemosa genes. Catuaí Vermelho IAC 99 cultivar, which is a pure C. arabica, was used as a susceptible control. In conditions of high natural infestation of red mite, the resistance of coffee genotypes was evaluated using a standard visual evaluation in January 2017. This evaluation was based on the percentage of leaf area with typical symptoms caused by this mite. Only one genotype, the F4 line IAPAR H0113-40-26-10 showed resistance to the red mite because present less leaf area with symptoms compared to other genotypes. The Arabica coffee cultivars IPR 99, IPR 100 and IPR 103 are as susceptible to red mite as to the susceptible control Catuaí Vermelho IAC 99.

scholarly journals POTENTIAL OF ACEH ARABICA COFFEE EXTRACT (Coffea arabica L.) IN REJUVENATION OF AGING SKIN IN RAT

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Negative Control ◽  
Arabica Coffee ◽  
Treatment Groups ◽  
Randomized Design ◽  
Skin Collagen ◽  
Completely Randomized Design ◽  
Skin Quality ◽  
Average Skin ◽  
Coffea Arabica L ◽  
Aging Skin

This study aimed to u determine the potency of Aceh Arabica coffee In rejuvenation of aging skin in rat. This study was conducted in vivousing completely randomized design (RAL), which consisted of 4 treatment groups as follows: premenopausal rats as negative control (KON),premenopausal rats with administration of distilled water as placebo (PLS), premenopausal rats with administration of ethinylestradiol 9x10-3mg/day/200 g BW (EST), and premenopausal rats with coffee extract administration of 300 mg/day/200 g BW (KOP). Each treatment groupconsisted of 5 rats. The parameters observed were skin collagen, water content/skin hydration, and skin ribonucleic acid (RNA) level. The datawere analyzed using analysis of variance (ANOVA) and Duncan test using SAS 9.13 software. The result showed the average skin collagen andRNA levels were higher in premenopausal rats with administration of coffee extract and ethinylestradiol (P<0.05) than control rats. On the otherhand, average of water level in premenopausal rats with coffee extract and ethinylestradiol administration were the same as control rats. It can beconcluded that administration of Aceh Arabica coffee extract can repair skin quality, which is indicated by the increase of collagen and RNAlevel on aging skin in rat.

scholarly journals Effect of drying and/or warming piglets at birth under warm farrowing room temperatures on piglet rectal temperature over the first 24 h after birth

2021 ◽  
Author(s):  
Katherine D Vande Pol ◽  
Andres F Tolosa ◽  
Caleb M Shull ◽  
Catherine B Brown ◽  
Stephan A S Alencar ◽  
...  
Keyword(s):  
Birth Weight ◽  
Birth Control ◽  
Rectal Temperature ◽  
Fixed Effects ◽  
Random Effect ◽  
Repeated Measure ◽  
Experimental Unit ◽  
Concentration Data ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract Piglets experience a decline in body temperature immediately after birth, and both drying and warming piglets at birth reduces this. However, these interventions may have less effective at higher farrowing room temperatures. This study was carried out at a commercial facility to compare the effect of drying and/or warming piglets at birth on postnatal rectal temperature (RT) under relatively warm farrowing room temperatures (26.6 ± 2.09°C). Forty-five sows/litters were used in a completely randomized design to compare three Intervention Treatments (applied at birth): Control (no treatment); Warming (piglets placed in a plastic box under a heat lamp for 30 min); Drying+Warming (piglets dried with desiccant and warmed as above). Temperatures in the warming boxes over the study period averaged 37.7 ± 2.75°C. At birth, piglets were weighed; RT temperature was measured at 0, 10, 20, 30, 45, 60, 120, and 1440 min after birth. Blood samples were collected at 24 h after birth from a subsample of one piglet from each birth weight quartile within each litter to measure plasma immunocrit concentration. Data were analyzed using PROC MIXED of SAS with litter as the experimental unit; and piglet a subsample of litter. The model for analysis of piglet rectal temperature included fixed effects of treatment, measurement time (repeated measure), the interaction, and the random effect of sow. Compared to the Control, piglet RT were higher (P ≤ 0.05) for the Warming treatment between 10 and 60 min, and higher (P ≤ 0.05) for the Drying+Warming treatment between 10 and 120 min after birth. Rectal temperatures were higher (P ≤ 0.05) for the Drying+Warming than the Warming treatment between 20 and 120 min. Responses to drying and/or warming were greater for low birth weight piglets (&lt; 1.0 kg) than heavier littermates, but were generally less than observed in previous experiments with similar treatments carried out under cooler temperatures. Piglet immunocrit values were lower (P ≤ 0.05) for the Drying+Warming treatment compared to the other treatments, which were similar (P &gt; 0.05). Immunocrit values tended (P = 0.10) to be lower for light (&lt; 1.0 kg) compared to heavier birth weight piglets. In conclusion, drying and warming piglets at birth was more effective for reducing piglet RT decline after birth than warming alone, though the effect was less than observed in previous studies carried out under cooler farrowing room temperatures.

scholarly journals Digestibility and bioavailability of phosphorus from spray-dried yeasts in the diet of starting pigs (15-30 kg)

2013 ◽  
Vol 14 (2) ◽  
pp. 308-321
Author(s):  
Lina Maria Peñuela Sierra ◽  
Ivan Moreira ◽  
Antonio Claudio Furlan ◽  
Paulo Levi de Oliveira Carvalho ◽  
Ângela Rocio Poveda Parra ◽  
...  
Keyword(s):  
Live Weight ◽  
Basal Diet ◽  
Relative Bioavailability ◽  
Mean Value ◽  
Experimental Unit ◽  
Available Phosphorus ◽  
Standard Curve ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Spray Dried

Two experiments were carried out to determine the bioavailability of phosphorus in two spray-dried yeasts - sugarcane yeast (SCY), and sugarcane yeast + brewer's yeast (SCBY) - in starting pigs, by comparing different methods (Apparent Digestibility Coefficient of Phosphorus - ADCP; True Digestibility Coefficient of Phosphorus - TDCP; slope ratio; and standard curve). In experiment I, a digestibility assay were carried out using 30 cross breed pig with initial weigh of 22.69 ± 4.24kg, allotted in a completely randomized design. The mean ADCP and TDCP values were 62.68 and 64.15% for SCY and 77.01 and 79.33% for SCBY. ADCP and TDCP for SCBY were higher (P<0.05) than the values for SCY. In Experiment II, a growth test was conducted, 56 crossbred piglets, were utilized, with initial live weight of 15.11 ± 3.43kg, allotted in a completely randomized design, with seven treatments, four replications, and two pigs per experimental unit. The treatments consisted of a basal diet without supplementation with P and the same diet including supplementation with two levels of P (0.053% and 0.105%) from dicalcium phosphate, SCY and SCBY. The relative bioavailability mean value of 57.23% for SCY and 91.96% for SCBY, corresponding to 0.30% and 0.40% of available phosphorus, respectively.

scholarly journals Resistance to nematode Meloidogyne paranaensis in Arabica coffee genotypes introgressed with Coffea liberica

2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Mother Plants ◽  
Completely Randomized Design ◽  
Set Up ◽  
Moderately Resistant ◽  
Reproduction Factor ◽  
Coffea Liberica

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.

scholarly journals Broccoli yield in response to top-dressing fertilization with green manure and biofertilizer

Revista CERES ◽  
2011 ◽  
Vol 58 (5) ◽  
pp. 645-650 ◽  
Author(s):  
Gilberto Bernardo de Freitas ◽  
Márcio Sousa Rocha ◽  
Ricardo Henrique Silva Santos ◽  
Letícia Monteiro da Silva Freitas ◽  
Leandro de Almeida Resende
Keyword(s):  
Green Manure ◽  
Mineral Fertilizer ◽  
Dry Mass ◽  
Experimental Unit ◽  
Productive Performance ◽  
Mineral Fertilization ◽  
Randomized Design ◽  
Protected Cultivation ◽  
Completely Randomized Design ◽  
Organic Compost

The objective of this work was to evaluate the productive performance of broccoli under different top-dressing organic fertilizations. The experiment was conducted under protected cultivation, in a completely randomized design with four replications, with two plants per experimental unit. Broccoli seedlings were produced in a commercial substrate in styrofoam trays. The seedlings were transplanted to plastic pots containing 10.0 L of substrate made up of subsoil and organic compost at the ratio of 3:1 (v/v), respectively, which is equivalent to about 20.0 t ha-1 of organic compost at planting. After seedling establishment, the top-dressing fertilization treatments were applied: gliricidia biomass associated or not with liquid biofertilizer of cattle manure to the soil and bokashi. Two control treatments were established: one with mineral fertilization recommended for the crop and the other without top-dressing fertilization. The broccoli production was evaluated (commercial standard). Plants that received mineral fertilizer were more productive, however, they were not significantly different (p>0.05), by Dunnet test, from the plants fertilized with 2.5 t ha-1 gliricidiabiomass (dry mass) associated with liquid biofertilizer (2.0 L m-2) applied to soil. Top-dressing fertilizations with only gliricidia, at 2.5 and 5.0 t ha-1 of biomass (dry mass), resulted in no significant increase in production of broccoli inflorescence. The use of bokashi in addition to gliricidia biomass and liquid biofertilizer reduced the efficiency of the fertilization compared with plants that received only gliricidia and liquid biofertilizer.

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