Influence of temperature on the activity of anammox granular biomass

2016 ◽  
Vol 73 (10) ◽  
pp. 2518-2525 ◽  
Author(s):  
D. Sobotka ◽  
K. Czerwionka ◽  
J. Makinia

The aim of this study was to determine a short-term and long-term effect of temperature on the anammox rate and determination of temperature coefficients in the Arrhenius and Ratkowsky equations. The short-term effects of temperature on the anammox granular biomass were investigated in batch tests at ten different temperatures in the range of 10–55 °C. The maximum overall nitrogen removal rate of 1.3 gN gVSS−1·d−1 was observed at 40 °C (VSS: volatile suspended solids). The minimum rate, close to 0 gN gVSS−1·d−1, was observed for the limits of the analyzed temperature range (10 and 55 °C). The activity tests carried out at 55 °C showed an irreversible loss of the activity due to the observed biomass lysis. Subsequently to the batch tests, a sequencing batch reactor (SBR) was operated at different temperatures (from 30 to 11 °C) to determine the long-term effects of temperature. The system was successfully operated at 15 °C, but when temperature was decreased to 11 °C, nitrite started to accumulate and the system lost its stability. The temperature coefficient (θ) was 1.07 for the batch tests carried out in the temperature range of 10–40 °C. In contrast, during the long-term SBR operation, substantially different θ had to be estimated for two temperature ranges, 1.07 (T = 15–30 °C) and 1.65 (T = 11–15 °C).

Energies ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3961
Author(s):  
Hussein Al-Hazmi ◽  
Xi Lu ◽  
Dominika Grubba ◽  
Joanna Majtacz ◽  
Przemysław Kowal ◽  
...  

The short-term effects of temperature on deammonification sludge were evaluated in a laboratory-scale sequencing batch reactor (SBR). Mathematical modeling was used for further evaluations of different intermittent aeration strategies for achieving high and stable deammonification performance at decreasing temperatures. As for the biomass cultivated at high temperatures (e.g., 30 °C), a higher temperature dependency (the adjusted Arrhenius coefficient θ for 11–17 °C = 1.71 vs. θ for 17–30 °C = 1.12) on the specific anammox growth rates was found at lower temperatures (11–17 °C) in comparison with higher temperatures (17–30 °C). Further evaluations of recovering the nitrogen removal efficiency at decreasing temperatures with the mathematical model by modifying the intermittent aeration strategies (aeration frequency (F) and the ratio (R) between non-aerated (non-aer) phase and aerated (aer) phase durations) indicated that intermittent aeration with a prolonged non-aerated phase (e.g., R ≥ 4 regardless of F value) would help to maintain high and stable deammonification performance (~80%) at decreasing temperatures (14–22 °C). Extending the non-aerated phases (increasing R) and reducing the frequency (F) of off/on phase changes have a positive effect on increasing energy savings, leading to increasing interest in this method.


2008 ◽  
Vol 58 (5) ◽  
pp. 1113-1120 ◽  
Author(s):  
S. E. Vlaeminck ◽  
L. F. F. Cloetens ◽  
M. Carballa ◽  
N. Boon ◽  
W. Verstraete

A novel and efficient way of removing nitrogen from wastewater poor in biodegradable organic carbon, is the combination of partial nitritation and anoxic ammonium oxidation (anammox), as in the one-stage oxygen-limited autotrophic nitrification/denitrification (OLAND) process. Since anoxic ammonium-oxidizing bacteria grow very slowly, maximum biomass retention in the reactor is required. In this study, a lab-scale sequencing batch reactor (SBR) was used to develop granular, rapidly settling biomass. With SBR cycles of one hour and a minimum biomass settling velocity of 0.7 m/h, OLAND granules were formed in 1.5 months and the nitrogen removal rate increased from 50 to 450 mg N L−1 d−1 in 2 months. The granules had a mean diameter of 1.8 mm and their aerobic and anoxic ammonium-oxidizing activities were well equilibrated to perform the OLAND reaction. Fluorescent in-situ hybridization (FISH) demonstrated the presence of both β-proteobacterial aerobic ammonium oxidizers and planctomycetes (among which anoxic ammonium oxidizers) in the granules. The presented results show the applicability of rapidly settling granular biomass for one-stage partial nitritation and anammox.


Energies ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1624
Author(s):  
Nelson Thambiraj ◽  
Ivar Waernhus ◽  
Crina Suciu ◽  
Arild Vik ◽  
Alex C. Hoffmann

This paper studies the robustness of off-shore solid oxide fuel cell (SOFC) installations and the nature and causes of possible cell degradation in marine environments. Two important, cathode-related, impediments to ensuring SOFC reliability in off-shore installations are: cathode degradation due to salt contamination and oxygen depletion in the air supply. Short-term and long-term tests show the effect of salt contamination in the cathode feed on cell performance, and reveal the underlying cause of the degradation seen. SEM/X-ray Diffraction/(XRD) analyses made it possible to identify salt taken up in the cathode microstructure after the short-term testing while the macroscopic cell structure remained intact after the short-term tests. The long-term degradation was found to be more severe, and SEM images showed delamination at the cathode/electrolyte interface with salt present, something that was not seen after long-term testing without salt. The effect of oxygen depletion on the performance was also determined at three different temperatures using I-V curves.


2008 ◽  
Vol 58 (1) ◽  
pp. 239-245 ◽  
Author(s):  
M. Naso ◽  
A. Chiavola ◽  
E. Rolle

This paper provides new insights on the application of the ozonation process for the reduction of the activated sludge production in a sequencing batch reactor (SBR). The study was performed in two identical lab-scale SBRs plant, one for experimental activities (Exp SBR) and one used as control (Control SBR), both fed with domestic sewage. A fraction of the activated sludge collected from the Exp SBR at the end of the aerobic react phase was periodically subjected to ozonation for 30 minutes at three different specific dosages (0.05, 0.07 and 0.37 g O3/gSS) and then recirculated before the beginning of the following cycle. Recirculation of the ozonated sludge to the Exp SBR did not appreciably affect the efficiency of the biological nitrogen and carbon removal processes. Nonetheless, an improvement of the denitrification kinetic was observed. Mixed liquor volatile and suspended solids (MLSS and MLVSS, respectively) concentrations in the reactor decreased significantly with time for long term application of the ozonation treatment. Kinetic batch tests on unstressed sludge taken from Control SBR indicated that the different oxidant dosages (0.05, 0.07 and 0.37 g O3/gSS) and durations of the ozonation process (10, 20 and 30 minutes) used remarkably affected chemical oxygen demand (COD) and organic nitrogen fractioning. In particular, soluble and biodegradable fractions seemed to be higher at lower dosage and longer contact time.


Zygote ◽  
2015 ◽  
Vol 24 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Marcella Costa Radael ◽  
Leonardo Demier Cardoso ◽  
Dalcio Ricardo de Andrade ◽  
André Veloso Ferreira ◽  
Douglas da Cruz Mattos ◽  
...  

SummaryThe present study aimed to provide data on the time required for Melanotaenia boesemani to complete embryonic development, and to investigate the influence that incubation at different temperatures caused in this species. The effects of temperature on the time and hatching rate are presented, as well as information related to embryonic development stages. After fertilization, the eggs were kept in incubators at 23, 26, 29 or 32°C and observed at predetermined times until the moment of hatching. Stages of development were identified and classified according to morphological and physiological characteristics. Oil droplets were visualized inside the eggs as well as filament adhesion present at the chorion. Embryonic development was similar to that observed in other species of the genus Melanotaenia with hatching and faster development in higher temperatures.


2004 ◽  
Vol 36 (1) ◽  
pp. 11-20 ◽  
Author(s):  
S.A. Firstov ◽  
E.P. Pechkovsky

The ternary compound of titanium-siliceous carbide Ti3SiC2, one of the representatives of Nan laminates, prepared by solid-phase sintering is investigated in compact and porous (q=0.03-0.41) states. Features of its short-term and long-term hardness (?=10 N) behavior in the temperature range from 20 to 1200?? at a holding time of 1-60 min were studied. It is shown that a temperature of about 700?? and holding time under load of about 10 min are critical values of the indentation procedure that correspond to an intensive decrease of hardness. The presence of porosity results in a decrease in hardness. a deformation scheme of compact and porous titanium-siliceous carbide Ti3SiC2 in the temperature range from 20 to 1200?? is proposed. .


2010 ◽  
Vol 129-131 ◽  
pp. 230-234
Author(s):  
Ying Ying Zhang ◽  
Qi Lin Zhang ◽  
Chuan Zhi Zhou ◽  
Ying Zhou

As composite, the mechanical properties of coated fabrics are sensitive to environment. This paper presented mechanical properties under different environments. A list of uniaxial tests are carried out under different temperatures including -20°C, 0°C, 23°C, 50°C, and 70°C. First, the tensile behaviors at room temperature and the failure behaviors are studied. Then, the effects of temperature on mechanical properties are determined. Finally, the effects of water immersion on mechanical properties are discussed. Results show PTFE coated fabrics remained unchanged in varying temperature and humidity. The temperature has effects on the mechanical properties of PVC coated fabrics. With increasing temperature, the strength decrease and the strain at break increase. The temperature induction factors are proposed for the design and analysis. The water immersion has little effect on the mechanical properties because of the impervious coating.


2009 ◽  
Vol 60 (1) ◽  
pp. 9-17 ◽  
Author(s):  
A. Donoso-Bravo ◽  
C. Retamal ◽  
M. Carballa ◽  
G. Ruiz-Filippi ◽  
R. Chamy

The effect of temperature on the kinetic parameters involved in the main reactions of the anaerobic digestion process was studied. Batch tests with starch, glucose and acetic acid as substrates for hydrolysis, acidogenesis and methanogenesis, respectively, were performed in a temperature range between 15 and 45°C. First order kinetics was assumed to determine the hydrolysis rate constant, while Monod and Haldane kinetics were considered for acidogenesis and methanogenesis, respectively. The results obtained showed that the anaerobic process is strongly influenced by temperature, with acidogenesis exerting the highest effect. The Cardinal Temperature Model 1 with an inflection point (CTM1) fitted properly the experimental data in the whole temperature range, except for the maximum degradation rate of acidogenesis. A simple case-study assessing the effect of temperature on an anaerobic CSTR performance indicated that with relatively simple substrates, like starch, the limiting reaction would change depending on temperature. However, when more complex substrates are used (e.g. sewage sludge), the hydrolysis might become more quickly into the limiting step.


2007 ◽  
Vol 18 (4) ◽  
pp. 313-315 ◽  
Author(s):  
Xiao-rui Guo ◽  
Lei Yang ◽  
Jing-hua Yu ◽  
Zhong-hua Tang ◽  
Yuan-gang Zu

2016 ◽  
Vol 1 ◽  
pp. 6 ◽  
Author(s):  
Lily D. Poulikakos ◽  
Bernhard Hofko ◽  
Laurent Porot ◽  
Xiaohu Lu ◽  
Hartmut Fischer ◽  
...  

Properties of asphalt concrete after aging are important parameters in determining the long-term performance of these materials. With the popularity of reduced temperature mixtures the question remains how this reduced temperature in short-term aging affects the long-term properties. This paper focuses on developing a robust and fundamental understanding of the effect of temperature on aging by connecting the chemistry of bitumen to its mechanics. To this end, round robin experiments are being currently conducted within 8 laboratories using four binders of the same grade 70/100 pen from different crude sources. In developing chemo-mechanical characterization techniques at the nano- and micro-scale, the material’s variability from crude-source to crude-source and its sensitivity to temperature needs to be taken into consideration. Furthermore, the development of uniform specimen preparation procedures for these bituminous materials at the nano- and micro-structural level is the focus. The chemical characterization is performed using Fourier transform infrared spectroscopy (FTIR). For the mechanical characterization rheological data is used using the dynamic shear rheometer (DSR) as well as conventional tests, e.g. needle penetration and softening point using the ring and ball method. It is shown that the short-term aging temperature affects the increase in softening point, while the consequent effect on long term aging is less pronounced.


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