Analyzing the Amperometric Response of a Glucose Oxidase Sensor Applying Mathematical Models

2016 ◽  
Vol 12 (6) ◽  
pp. 634-641 ◽  
Author(s):  
Felix F. González Navarro ◽  
Margarita Stoytcheva ◽  
Lluís A. Belanche Munoz ◽  
Brenda L. Flores Rios ◽  
Jorge E. Ibarra Esquer ◽  
...  
Keyword(s):  
Mathematical Models ◽  
Glucose Oxidase ◽  
Amperometric Response

Glucose Biosensor Based on Pt Nanoparticles/Graphene Chitosan Bionanocomposites

2013 ◽  
Vol 328 ◽  
pp. 695-699 ◽  
Author(s):  
Hui Ping Liu ◽  
Gong Yong Zhan ◽  
Qi Zhi Dong ◽  
Yan An Lv ◽  
Jian Fang Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Hybrid Film ◽  
Pt Nanoparticles ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Redox Behavior ◽  
Amperometric Response ◽  
Scanning Electron

In this paper, we fabricated a bionanocomposite film of glucose oxidase/Pt nanoparticles/graphene-chitosan (GOD/PtNPs/GR-Chit) for glucose sensing. The hybrid bionanocomposites modified GCE were characterized by scanning electron microscopy (SEM), cyclic voltammetry, and amperometric i-t curve. It was found that the PtNPs were uniformly deposited on the surface of GR-Chit hybrid film. The resultant PtNPs/GR-Chit/GCE exhibited a high electrochemical catalytic ability to hydrogen peroxide (H2O2), due to the electrocatalytic synergy of GR and PtNPs. The redox behavior of the GOD/PtNPs/GR-Chit/GCE is a surface-controlled process. Finally, we obtained the amperometric response of the GOD/PtNPs/GR-Chit/GCE toward different concentration of glucose, and also achieved a sensitive glucose oxidase biosensor with a detection limit of 4.6μM glucose.

scholarly journals Fabrication of Biosensor Based on Polyaniline/Gold Nanorod Composite

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Uğur Tamer ◽  
Ali İhsan Seçkin ◽  
Erhan Temur ◽  
Hilal Torul
Keyword(s):  
Glucose Oxidase ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Gold Nanorod ◽  
Sers Spectrum ◽  
Pani Film ◽  
Amperometric Response ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

This present paper describes a new approach to fabricate a new amperometric sensor for the determination of glucose. Polyaniline (PANI) film doped with colloidal gold nanorod particles has been used to immobilize glucose oxidase by glutaraldehyde. The polyaniline/gold nanorod composite structure gave an excellent matrix for enzyme immobilization due to the large specific surface area and higher electroactivity. The composite has been characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), and surface-enhanced Raman spectroscopy (SERS). The SERS spectrum of the surface-immobilized glucose oxidase and the spectrum of the native enzyme indicate that the main feature of the native structure of glucose oxidase was conserved after being immobilized on the polymer matrix. The amperometric response was measured as a function of concentration of glucose at a potential of 0.6 V versus Ag/AgCl in 0.1 M phosphate buffer at pH 6.4. Linear range of the calibration curve was from 17.6 μM to 1 mM with a sensitivity of 13.8 μA⋅mM−1⋅cm−2and a limit of detection (LOD) of 5.8 μM. The apparent Michaelis-Menten constantKMwas calculated as 1.0 mM and the response time was less than 3 seconds.

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