scholarly journals The Effects of Plant Leaves Variants from The Philippines on Infective Oesophagustonum dentatum Larvae

2019 ◽  
Vol 1 (1) ◽  
pp. 9-18
Author(s):  
Maria Asuncion G. Beltran ◽  
◽  
Allan Robertson ◽  
Richard J. Martin ◽  
◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Room Temperature ◽  
Moringa Oleifera ◽  
Gliricidia Sepium ◽  
The Philippines ◽  
Ananas Comosus ◽  
Aqueous Extracts ◽  
Plant Leaves ◽  
Annona Squamosa ◽  
Chrysophyllum Cainito

Nine aqueous extracts of plant leaves from the Philippines were evaluated against Oesaphagustomum dentatum sensitive (ODSEns) larvae for their anthelmintic effects. The plant species evaluated were Ananas comosus, Chrysophyllum cainito,Gliricidia sepium, Annona squamosa, Moringa oleifera, Tinospora rumphii Boerl,Azadirachta indica, Trichantera gigantea and Leuacaena leucocephala. The leaves of the plants gathered from Camiling, Tarlac, Philippines, were dried under room temperature for 30 days and then ground. Aqueous extracts of these plant leaves were prepared using migration buffer. In vitro migration and inhibition assays were used to evaluate the anthelmintic activity against exsheathed third stage larvae of Oesophagustomum dentatum. The experiments were put in 24-well plates at room temperature with four replications per treatment using different concentrations of 1, 2.5, 5 and 10 mg/ml. About 120 larvae per well were deposited on a larval migration apparatus consisting of 20 μm nylon mesh filters and incubated 20 hours under different treatments and another 2 hours to allow the migration of active motile larvae. The evaluation criteria were based on the average numbers of inhibited larvae under the different treatments. The percentage inhibition were statistically analyzed using Two-Way ANOVA (P=≤.0001) of the Graph Pad Prizm software (V5 San Diego, Ca). The most effective leaves with their highest inhibition level were from Ananas comosus (pineapple, 86.50%), Gliricidia sepium (madre de cacao, 84.50%), Chrysophyllum cainito (star apple, 83.75%), Moringa oleifera (horseradish,58.75%), Annona squamosa (sweet sop, 56.00%), Tinospora rumphii Boerl ( \Makabuhay, 55.75%), and Azadirachta indica (Neem, 51.50%), whereby extremely significant (P˂0.001) effect against O. dentatum larvae. Two leaves extracts, from Trichantera gigantea and Leuacaena leucocephala have no significant effect on O. dentatum larvae. The larval migration assay allows initial studies on the anthelmintic effects of the herbs.

scholarly journals COMPARATIVE ANALYSIS OF THE PHYTOCHEMICAL COMPOSITIONS OF LEAF, STEM-BARK AND ROOT OF AZADIRACHTA INDICA (NEEM)

2018 ◽  
Author(s):  
Nwali ON. ◽  
Idoko A. ◽  
Okolie JE ◽  
Ezeh E ◽  
Ugwudike PO ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Room Temperature ◽  
Reducing Sugars ◽  
Stem Bark ◽  
Aqueous Extracts ◽  
Leaf Sample ◽  
Plant Parts ◽  
Physico Chemical ◽  
Antifungal Action ◽  
Different Parts

Plant chemicals abound in different parts of plants, in different compositions. Thus, the comparative screening of the leaf, stem-bark and root of Azadirachta Indica becomes imperative. The presence of nine phytochemicals which include; Alkaloids, Flavonoids, Saponins, Glycosides, Phenols, Steroids, Tannins, Reducing sugars and Anthraquinones, and the quantity of alkaloids, flavonoids, saponins, tannins and glycoside in the leaf, stem-bark and root of Azadirachta indica were investigated. The plant parts were collected from a plantation grown in Okpotegu Echara, Ikwo Local Government Area of Ebonyi State, Nigeria. Plant samples were  air dried at room temperature, ground into uniform powder, sieved, bottled and labeled, ready for physico – chemical analysis. Results of the investigation revealed that anthraquinones were beyond detection limits in all the plant parts tested in both ethanol and aqueous extracts. Alkaloids were not detectable in leaf, stem-bark and root samples of aqueous extract. Glycosides were not detectable in leaf sample of ethanol and aqueous extracts. Quantitatively, the phytochemical compositions of each part showed higher concentrations of Alkaloids in the leaf, stem-bark and root (11.63%, 4.93% and 3.79%), compared to flavonoids (2.19%, 2.72% and 0.92%), saponins (0.70%, 1.12% and 0.44%), tannins (0.33 mg/100, 0.50mg/100 and 0.17mg/100) and glycosides (0.23%, 0.27% and 0.19%), respectively. Obviously, except for the higher percentage (11.63%) of alkaloids in the leaf, the phytochemicals in the stem-bark are higher as shown by the results, which could support the reason that the bark is preferably chewed commonly together with the stem as chew stick for its germicidal and antifungal action.

scholarly journals Prospects of botanical pesticides in management of Iroko gall bug, Phytolyma fusca (Hemiptera, Psylloidea) under laboratory and field conditions

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Juliana Amaka Ugwu
Keyword(s):  
West Africa ◽  
Azadirachta Indica ◽  
Residual Effects ◽  
Aqueous Extracts ◽  
Plant Materials ◽  
Piper Guineense ◽  
Early Management ◽  
Ethanol Extracts ◽  
Milicia Excelsa ◽  
Contact Effects

Abstract Background Iroko gall bug, Phytolyma fusca Walker, is a major insect pest of Milicia excelsa (Iroko) seedling hampering its propagation in West Africa. Milicia excelsa is an indigenous forest timber tree in the tropical rain forest of West Africa with a very high value in international trade due to its wood quality. Sustainable management of P. fusca infestations on Iroko seedlings have not been achieved due to their cryptic nature and multivoltine generations. This study evaluated the residual and contact effects of crude ethanol and aqueous extracts of four plants (Azadirachta indica, Jatropha curcas, Piper guineense, and Aframomum melegueta) on adult P. fusca in the laboratory and field. Results All the extracts had residual effects and contact effects on adult insects in the laboratory at 75% and 100% concentrations of applications. Azadirachta indica, P. guineense, and A. melegueta gave 80–100% adult mortality at both concentrations in the laboratory; their efficacies were comparable to cypermethrin. The ethanol extracts of P. guineense and A. indica were more effective than other extracts in protecting the seedlings against Phytolyma infestations in the field. They significantly (p < 0.01) reduced infestation compared to other extracts and control. Ethanol extracts of the tested plant materials were more effective than their aqueous extracts both in the laboratory and field. Conclusion The results proved that P. guineense and A. indica extracts were very potent and promising in protecting Milicia excelsa seedlings against Phytolyma fusca infestations and they can be used in the early management of Phytolyma infestations in the field.

scholarly journals THE ANTHELMINTIC EFFECTS OF ETHANOL EXTRACT OF CURANGA FEL-TERRAE LEAVES ON ASCARIDIA GALLI

2017 ◽  
Vol 10 (3) ◽  
pp. 117 ◽  
Author(s):  
Popi Patilaya ◽  
Dadang Irfan Husori ◽  
Imam Bagus Sumantri
Keyword(s):  
Sodium Chloride ◽  
Plant Extract ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ascaridia Galli ◽  
Plant Leaves ◽  
Sodium Carboxymethylcellulose ◽  
Solvent Control

ABSTRACTObjective: This study was to investigate the anthelmintic effects of ethanol extract of Curanga fel-terrae leaves on Ascaridia galli.Methods: Extract of C. fel-terrae was prepared by percolating the dried powder of the plant leaves. To observe anthelmintic effects, A. galli wasexposed to the plant extract solutions in different concentrations at room temperature for 72 hrs. Sodium chloride 0.9%, sodium carboxymethylcellulose 0.5%, and albendazole 0.1% were served as negative control, solvent control, and positive control, respectively. The anthelmintic effectswere determined by observing the time taken for paralysis and the time taken for death of A. galli during experiment.Results: The results demonstrated that there were no different effects between negative and solvent controls. However, the ethanol extract ofC. fel-terrae leaves exhibited significantly paralysis and death effects toward A. galli. The plant extracts at the concentration of 100 mg/ml showedsimilar anthelmintic effects with albendazole 0.1%. This study also indicated that the paralysis and death effects of plant extract were shorter whenthe worms exposed with the concentration above of 100 mg/ml.Conclusion: This study indicated that the ethanol extract of C. fel-terrae leaves has potential anthelmintic effects on A. galli.Keywords: Curanga fel-terrae, Ascaridia galli, Anthelmintic, Helminthiasis, Ascariasis. 

scholarly journals The Study of Pineapple Peel (Ananas comosus L.) Waste Based-Electrolyte Medium: A Simple Experiment Design for the Students

2019 ◽  
Vol 2 (2) ◽  
pp. 89
Author(s):  
Irvan Maulana Firdaus ◽  
Rosiana Dewi Silvia ◽  
Ahmad Faqih Amin ◽  
Rajwa Vourza Tsaqifa ◽  
Ira Purnama ◽  
...  
Keyword(s):  
Room Temperature ◽  
Electrode Materials ◽  
Oxidation Process ◽  
Operating Time ◽  
Normal Pressure ◽  
Ananas Comosus ◽  
Simple Experiment ◽  
Measured Voltage ◽  
Turn On ◽  
Pineapple Peel

<p>To promote the student curiosity and understanding in the electrolyte medium was carried out using home-made Volta cell. The electrode materials were iron and carbon for anode (-) and cathode (+), respectively. The experiment was designed by two models that were single-chamber (SC) and three series-packed chambers (3-SCs), respectively. Electrolyte properties could be investigated in pineapple peel (<em>Ananas comosus </em>L.)-based electrolyte medium during the operating time of 8 hours, respectively. The measured-voltage of 2.63 and 2.60 of the 3-SCs system could turn on the LED lamp. However, in this study, the decrease of voltage and current were due to the oxidation process of the pineapple peel-based medium under air, room temperature, and normal pressure during the long-operating time of the experiment. Finally, this research expected to provide additional valuable experience and knowledge as same as to facilitate in information delivering to the students in understanding the electrolyte medium from the waste or natural sources.</p>

Effect of dietary supplementation with powder derived from Moringa oleifera and Azadirachta indica leaves on growth and microbial load of edible crickets

2021 ◽  
pp. 1-14
Author(s):  
J. Ng’ang’a ◽  
S. Imathiu ◽  
F. Fombong ◽  
J. Vanden Broeck ◽  
J. Kinyuru
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Azadirachta Indica ◽  
Moringa Oleifera ◽  
Antibacterial Properties ◽  
Acheta Domesticus ◽  
The Body ◽  
Microbial Load ◽  
Field Cricket ◽  
Bacterial Endospores

Cricket farming has shown its potential to address food and nutrition insecurity in parts of the world. However, one of the remaining challenges limiting mass production of edible insects is the affordability of feeds. One possible way to address this issue would be to explore the use of traditional plants such as Moringa oleifera leaf (MOL) and Azadirachta indica leaf (AIL) that are locally available, are protein-rich and possesses some antibacterial properties in insect feed formulation. In this regard, a study was conducted to evaluate the effect of supplementation of MOL (5 or 10%) and AIL (5 or 10%) powder in the commercially used starter chicken feed on growth and microbial load of house cricket (Acheta domesticus) and field cricket (Gryllus bimaculatus). After feeding for four weeks, the supplemented feeds with 10% MOL or 10% AIL in A. domesticus and G. bimaculatus significantly decreased (P<0.05) the body weights. Also, supplementation with MOL (5 or 10%) and AIL (5 or 10%) increased the mortality in both cricket species. Although high counts of total aerobic, Enterobacteriaceae, lactic acid bacteria, bacterial endospores, yeasts and moulds were observed, significantly lower counts (P<0.05) of lactic acid bacteria and bacterial endospores were observed in treatments containing MOL (5 or 10%) and 10% AIL in both cricket species. After processing (toasting and boiling), significant reductions (P<0.001) of all vegetative microbial cells were observed but bacterial endospores were not completely eliminated. Thus, during production and processing of insects and insect-derived products, bacterial endospores would require special attention. Salmonella and Escherichia coli were not detected in processed crickets’ samples. In conclusion, supplementation with 5% MOL or 5% AIL can produce crickets with similar body weight as control feed. Mortality increased significantly when feeds were supplemented with either MOL or AIL powder. Supplementation with MOL and AIL powders significantly reduced the cell numbers for some bacterial groups in both cricket species.

Effects of diet fortification with Azadirachta indica and Moringa oleifera leaf meal on parasitaemia, performance, rectal temperature and serum biochemistry of rabbits infected with Trypanosoma brucei brucei

2021 ◽  
Vol 48 (2) ◽  
pp. 101-110
Author(s):  
A. O. Salifu ◽  
S. N. Carew ◽  
A. J. Dadah ◽  
M. Adamu
Keyword(s):  
Rectal Temperature ◽  
Azadirachta Indica ◽  
Total Protein ◽  
Moringa Oleifera ◽  
Line Graph ◽  
Serum Biochemistry ◽  
Design Parameters ◽  
Feed Conversion ◽  
Randomized Design ◽  
Percent Mortality

Numerous plants are reported to have ethno-therapeutic properties; among these plants are the leaves of Moringa oleifera and Azadirachta indica used for the treatment of protozoan infections. Forty grower rabbits at approximately 70 days old were used to determine the prophylactic anti-trypanosomal effects of the dry leaf meals of Moringa oleifera (MLM) and Azadirachta indica (NLM). The rabbits were placed into eight groups of five animals each in a completely randomized design, with mean live weights of 1.2 Kg. The rabbits were allotted to four diets (D1, D2, D3 and D4) and two levels of inoculation (inoculated and uninoculated rabbits) in a 4×2 factorial arrangement of a Completely Randomised Design. Parameters monitored were performance, serum biochemistry and rectal temperature. The parasitaemia were presented as line graph for the infected rabbits. The percent mortality of the groups was evaluated and no mortality was observed for rabbits fed D4. No significant differences (P>0.05) were observed between treatments for growth rate, feed intake and feed conversion, except rectal temperature and total protein (P<0.05) in serum biochemistry. Rabbits that were uninfected with trypanosomes had (P<0.05) lower total protein than the infected animals except T2. It was concluded that only the 1% MLM had prophylactic anti- trypanosomal effect on serum total protein and mortality amelioration of T. b. brucei infected rabbits when administered alone and a combination of both MLM and NLM prevented  pyrexia and mortality.     De nombreuses plantes auraient des propriétés ethno-thérapeutiques ; parmi ces plantes figurent les feuilles de Moringaoleifera et d'Azadirachtaindica utilisées pour le traitement des infections protozoaires. Quarante lapins de culture à environ 70 jours ont été employés pour déterminer les effets anti-trypanosomal prophylactiques des repas secs de feuille de Moringaoleifera (le 'MLM') et d'Azadirachtaindica (le 'NLM'). Les lapins ont été placés en huit groupes de cinq animaux chacun dans une conception complètement randomisée, avec des poids vivants moyen de 1,2 kg. Les lapins ont été attribués à quatre régimes (D1, D2, D3 et D4) et deux niveaux d'inoculation (lapins inoculés et non ininoculés) dans un arrangement factorial de 4×2 d'un Désigne complètement randomisé. Les paramètres surveillés étaient la performance, la biochimie sérique et la température rectale. La parasitémie a été présentée comme graphique linéaire pour les lapins infectés. La mortalité en pourcentage des groupes a été évaluée et aucune mortalité n'a été observée chez les lapins nourris au D4. Aucune différence significative (P>0,05) n'a été observée entre les traitements pour le taux de croissance, l'apport alimentaire et la conversion des aliments pour animaux, à l'exception de la température rectale et de la protéine totale (P<0,05) dans la biochimie sérique. Les lapins qui n'étaient pas infectés par les trypanosomes avaient (P<0,05) moins de protéines totales que les animaux infectés, à l'exception du T2. On l'a conclu que seulement le MLM de 1% a eu l'effet anti-trypanosomal prophylactique sur la protéine totale de sérum et l'amélioration de mortalité des lapins infectés de T.b. brucei une fois administrés seuls et une combinaison de MLM et de NLM a empêché la pyrexie et la mortalité.

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