scholarly journals Myeloperoxidase Interacts with Endothelial Cell-Surface Cytokeratin 1 and Modulates Bradykinin Production by the Plasma Kallikrein-Kinin System

2007 ◽  
Vol 171 (1) ◽  
pp. 349-360 ◽  
Author(s):  
Joshua M. Astern‡ ◽  
William F. Pendergraft ◽  
Ronald J. Falk‡ ◽  
J. Charles Jennette‡ ◽  
Alvin H. Schmaier ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Plasma Kallikrein ◽  
Kinin System ◽  
Endothelial Cell Surface ◽  
Kallikrein Kinin System

scholarly journals Plasma kallikrein‐kinin system and endothelial cell activation

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Zia Shariat‐Madar ◽  
Jia Yang ◽  
Noah Osman ◽  
Sean M Wilson
Keyword(s):  
Endothelial Cell ◽  
Cell Activation ◽  
Plasma Kallikrein ◽  
Endothelial Cell Activation ◽  
Kinin System ◽  
Kallikrein Kinin System

scholarly journals Surface charge distribution on the endothelial cell of liver sinusoids.

1984 ◽  
Vol 99 (2) ◽  
pp. 639-647 ◽  
Author(s):  
L Ghitescu ◽  
A Fixman
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Liver Sinusoid ◽  
Electron Microscopic ◽  
Coated Pits ◽  
Endothelial Cell Surface ◽  
Liver Sinusoids ◽  
Surface Charge Distribution ◽  
Cationic Residues

The topography of the charged residues on the endothelial cell surface of liver sinusoid capillaries was investigated by using electron microscopic tracers of different size and charge. The tracers used were native ferritin (pl 4.2-4.7) and its cationized (pl 8.4) and anionized (pl 3.7) derivatives, BSA coupled to colloidal gold (pl of the complex 5.1), hemeundecapeptide (pl 4.85), and alcian blue (pl greater than 10). The tracers were either injected in vivo or perfused in situ through the portal vein of the mouse liver. In some experiments, two tracers of opposite charge were sequentially perfused with extensive washing in between. The liver was processed for electron microscopy and the binding pattern of the injected markers was recorded. The electrostatic nature of the tracer binding was assessed by perfusion with high ionic strength solutions, by aldehyde quenching of the plasma membrane basic residues, and by substituting the cell surface acidic moieties with positively charged groups. Results indicate that the endothelial cells of the liver sinusoids expose on their surface both cationic and anionic residues. The density distribution of these charged groups on the cell surface is different. While the negative charge is randomly and patchily scattered all over the membrane, the cationic residues seem to be accumulated in coated pits. The charged groups co-exist in the same coated pit and bind the opposite charged macromolecule. It appears that the fixed positive and negative charges of the coated pit glycocalyx are mainly segregated in space. The layer of basic residues is located at 20-30-nm distance of the membrane, while most of the negative charges lie close to the external leaflet of the plasmalemma.

Sign in / Sign up

Export Citation Format

Share Document