scholarly journals The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

2018 ◽  
Vol 41 (2) ◽  
pp. 177-186
Author(s):  
Mustafa Sencer Karagul ◽  
Serkan Ikiz

Abstract The aim of this study is to evaluate the success of Brucella spp. isolation in ruminant abortion cases by using different selective media. To this end, 58 samples from ruminant abortion cases were utilized. 4 selective media; namely, Farrell Medium (FM), CITA Medium (CM), Modified Thayer Martin (MTM) and Jones & Morgan (JM) were preferred for isolation. In addition to these, one medium with antibiotics was used to extend the range of the results. Suspensions prepared from organ and fetal stomach contents were inoculated to media plates and incubated at 37C° for 5-8 days in 5-10% CO2 condition. Conventional biotyping method was used to identify Brucella isolates within the level of species and biovar. MTM (67.2%) and Farrell (65.5%) outperformed the other media with regards to isolation rate. However, regarding the inhibition ability against contaminant microrganisms, Farrell (86.2%) and CITA (72%) have the highest and second highest percentages respectively. The media’s inhibition ability was examined in the samples in which Brucella spp. isolation occurred to be able to investigate the correlations between isolation and inhibition. Lower isolation percentage was observed in the samples in which the media displayed the lowest inhibition ability against contaminants. In this context, using two different selective media with high inhibition ability against contaminants may be recommended to enhance the isolation rate. Moreover, the components stimulating the growth of Brucella strains might be added to the media to obtain better results.

2005 ◽  
Vol 53 (3) ◽  
pp. 275-285 ◽  
Author(s):  
L. Makrai ◽  
L. Fodor ◽  
I. Vendég ◽  
G. Szigeti ◽  
B. Dénes ◽  
...  

In order to improve the isolation rate of Rhodococcus equi from animals and soil, the efficacy of four previously described selective media (CAZ-NB, M3T, NANAT and TINSDALE) and that of four other media (NC, PNP, TCP and TVP) composed by us was compared and evaluated. Two selective plating media proved to be the best for the isolation of R. equi from contaminated samples. One of them was CAZ-NB containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed TCP containing trimethoprim, cefoperazone, polymyxin B, cycloheximide and potassium tellurite as selective components. These two media allowed the growth of at least 62-72% of R. equi present in the artificially contaminated samples, and the inhibition of unwanted contaminant bacteria and fungi was satisfactory with both media. TCP medium proved to be superior to CAZ-NB since the colony morphology of R. equi was much more characteristic (shiny, smooth, black colonies 3-5 mm in diameter) on it, and it inhibited the unwanted contaminant bacterial and fungal flora more effectively, especially in the case of faecal and soil samples. Therefore, TCP is recommended as a new, highly selective plating medium for the isolation of R. equi from contaminated samples.


2019 ◽  
Vol 4 (1) ◽  
pp. 1-19
Author(s):  
Neri Widya Ramailis ◽  
Dede Nopendri

Discourse is a series of sentences that relate and connect one proposition with the other propositions to from a unity. The main function of the news is not to warn, instruct, and make the public stunned, the main function of the news is to inform and then it is upto the public to utilize the news. There are two ways for the news to be useful to the public, the first to effort news as general knowledge and the second to effort the news a tool of social control. E-Ktp corruption cases are one of the biggest corruption cases that occurered in Indonesia. Therefore, many mass media reported heavilly on E-Ktp corruption cases, one of which was the kompas.com. furthermore, to find out how the writer gets the source the writer gets the source of data and information the writer uses the criminology visual method and then analyzes it using criminology newsmaking theory. However, the results of this study illustrate that the aspect highlighted are those of actors suspected of being involved in E-Ktp corruption cases. Where the media only emphasizes one institution, namely the people’s representative council, even though in this case the involved parties are not only the legislature but case the involved parties are not only the legislature but also from various institutions such as the interior ministry, state-owned enterprises, and private entrepreneurs. In the aspect of media projection Kompas.com make the bulk of the news about E- Ktp corruption cases as news headline and a tranding topic.


Author(s):  
Michael Ahmed

This paper re-evaluates the significance of Sir Curtis Seretse, a black character from the 1960s television series Department S (ITV 1969-70) which has largely been ignored. While earlier critical and academic discourse of Department S has primarily centred on the flamboyant Jason King, the importance of Seretse’s character has been overlooked. Seretse, as the head of Department S, is in a position of authority and power over the other (white) characters of the show. Furthermore, he represents a highly educated character that converses on equal terms with Prime Ministers and Presidents, a unique representation of a black character on British television at that time. Seretse’s appearance on prime time television, at a period when black performers in the media were invariably confined to little more than token characters, is therefore worthy of further attention. This paper examines how Seretse represents a different type of black character not previously seen on British television, when compared to the representations of racial problems on other television crime dramas.


2021 ◽  
pp. 216747952199237
Author(s):  
Ondřej Trunečka

This study explores how professional athletes perceive sports journalists and the role of the media. They acknowledge that communication with journalists is an essential part of their profession and appreciate the verified information, knowledge, and dutiful preparation. They consider the main role of media as monitorial. In some cases, they cast doubt on journalists’ ability to evaluate athletic performance accurately, and they are also critical of errors in interviews, however they acknowledgethat there are good, and possibly excellent journalists as well.


2021 ◽  
Vol 8 (3) ◽  
pp. 52
Author(s):  
Chanon Suntara ◽  
Anusorn Cherdthong ◽  
Metha Wanapat ◽  
Suthipong Uriyapongson ◽  
Vichai Leelavatcharamas ◽  
...  

Saccharomyces cerevisiae is a yeast strain often used to improve the feed quality of ruminants. However, S. cerevisiae has limited capacity to provide biomass when inoculated with carbon sources and a low ability to produce cellulase enzymes. Here, we hypothesized that yeast in the rumen produces a large amount of biomass and could release cellulase enzymes to break down fiber content. Therefore, the aim of this study was to screen, isolate and identify yeast from the rumen fluids of Holstein Friesian steers and measure the efficiency of biomass production and cellulase activity. A fermentation medium containing sugarcane molasses as a carbon source and urea as a nitrogen source was optimized. Two fistulated–crossbred Holstein Friesian steers averaging 350 ± 20 kg body weight were used to screen and isolate the ruminal yeast. Two experiments were designed: First, a 12 × 3 × 3 factorial was used in a completely randomized design to determine biomass and carboxymethyl cellulase activity. Factor A was the isolated yeast and S. cerevisiae. Factor B was sugarcane molasses (M) concentration. Factor C was urea (U) concentration. In the second experiment, potential yeasts were selected, identified, and analyzed for 7 × 4 factorial use in a completely randomized design. Factor A was the incubation times. Factor B was the isolated yeast strains, including codes H-Khon Kaen University (KKU) 20 (as P. kudriavzevii-KKU20), I-KKU20 (C. tropicalis-KKU20), and C-KKU20 (as Galactomyces sp.-KKU20). Isolation was imposed under aerobic conditions, resulting in a total of 11 different colonies. Two appearances of colonies including asymmetric colonies of isolated yeast (indicated as A, B, C, E, and J) and ovoid colonies (coded as D, F, G, H, I, and K) were noted. Isolated yeast from the rumen capable of providing a high amount of biomass when inoculant consisted of the molasses 15% + urea 3% (M15 + U3), molasses 25% + urea 1% (M25 + U1), molasses 25% + urea 3% (M25 + U3), and molasses 25% + urea 5% (M25 + U5) when compared to the other media solution (p < 0.01). In addition, 11 isolated biomass-producing yeasts were found in the media solution of M25 + U1. There were 4 isolates cellulase producing yeasts discovered in the media solution of M25 + U1 and M25 + U5 whereas molasses 5% + urea 1% (M5 + U1), molasses 5% + urea 3% (M5 + U3), molasses 5% + urea 5% (M5 + U5), molasses 15% + urea 1% (M15 + U1), molasses 15% + urea 3% (M5 + U3), and M25 + U3 were found with 2, 3, 1, 2, 1, and 2 isolates, respectively. Ruminal yeast strains H-KKU20, I-KKU20, and C-KKU20 were selected for their ability to produce biomass. Identification of isolates H-KKU20 and I-KKU20 revealed that those isolates belonged to Pichia kudriavzevii-KKU20 and Candida tropicalis-KKU20 while C-KKU20 was identified as Galactomyces sp.-KKU20. Two strains provided maximum cell growth: P. kudriavzevii-KKU20 (9.78 and 10.02 Log cell/mL) and C. tropicalis-KKU20 (9.53 and 9.6 Log cells/mL) at 60 and 72 h of incubation time, respectively. The highest ethanol production was observed in S. cerevisiae at 76.4, 77.8, 78.5, and 78.6 g/L at 36, 48, 60, and 72 h of incubation time, respectively (p < 0.01). The P. kudriavzevii-KKU20 yielded the least reducing sugar at about 30.6 and 29.8 g/L at 60 and 72 h of incubation time, respectively. The screening and isolation of yeasts from rumen fluids resulted in 11 different yeasts being obtained. The potential yeasts discovered in the rumen fluid of cattle were Pichia kudriavzevii-KKU20, Candida tropicalis-KKU20, and Galactomyces sp.-KKU20. P. kudriavzevii-KKU20 had higher results than the other yeasts in terms of biomass production, cellulase enzyme activity, and cell number.


Diagnostics ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 205
Author(s):  
Kou Tsuyama ◽  
Nobukazu Miyamoto ◽  
Atsuhiko Shindo ◽  
Kenichiro Hira ◽  
Yuji Ueno ◽  
...  

Duplication and accessory of the middle cerebral artery (MCA) constitute a rare congenital variation. MCA anomalies are found at a lesser frequency than the vascular anomalies of the other major intracranial arteries. Duplicated/accessory MCA was usually noted incidentally with subarachnoid hemorrhage, due to resulted aneurysmal formation. However, duplicated/accessory MCA-related cerebral infarction is rarer. We report two cases of cerebral infarction due to dissection at the entry of the duplicate/accessory MCA. Both cases were similar in dissected site and clinical course, without headache or injury. In 20 previously reported cases and our two cases of duplicated/accessory MCA-related infarction, mean age (55.8 ± 21.2 years) was slightly younger for cerebral infarction, and stroke etiology was mainly embolism. The main etiologies of stroke were embolism and dissection. Considering embolism etiology, proximal site of arterial diameter changing lesion was a common site for embolism, as duplicated/accessory MCA was usually smaller than normal M1 segment. In cerebral dissection cases, the dissected site was similar to our cases. Numerous mechanisms of dissection were considered, but they mainly included dysfunction of the media and endothelium or shearing stress at the entry of duplication. As the detailed mechanisms of cerebral dissection remain unknown, clinicians should include a differential diagnosis for MCA dissection.


Author(s):  
Dr. Jnanee Debasish Mishra

Synopsis: Literature is the medicine for man and civilization for years. It attracts and affects the soul and mind. In modern times transformation of media makes a big difference in human approach. Though Communication is the primary aim of media, but it works like a window of conscience. In the age of globalization the media has a great influence on society. Though market is an economical concept but our daily life is bound to rely upon it. And now literature, media and market remain in an inter related manner. One affects the other two. This analysis tries to find out the inter relationship among literature, media and market. Keywords: Literature, Media, Market, Communication, Globalization, Literary Sensibility, Media ethics, Change in media approach


Author(s):  
Ruth Grüters ◽  
Knut Ove Eliassen

AbstractTo understand the success of SKAM, the series’ innovative use of “social media” must be taken into consideration. The article follows two lines of argument, one diachronic, the other synchronic. The concept of remediation allows for a historical perspective that places the series in a longer tradition of “real time”-fictions and media practices that span from the epistolary novels of the 18th century by way of radio theatre and television serials to the new media of the 21st century. Framing the series within the current media ecology (marked by the connectivity logic of “social media”), the authors analyze how the choice of the blog as the drama’s media platform has formed the ways the series succeeded in affecting and mobilizing its audience. Given the long tradition of strong pedagogical premises in the teenager serials of publicly financed Norwegian television, the authors note the absence of any explicit media critical perspectives or didacticism. Nevertheless, the claim is that the media-practices of the series, as well as the actions and discourses of its followers (blogposts, facebook-groups, etc.), generate new insights and knowledge with regards to the series’ form, content, and practices.


2021 ◽  
Vol 3 (1) ◽  
pp. 667
Author(s):  
Risca Hidayanti Qurani ◽  
Safira Dewi Kurnia ◽  
Henny Dwi Bhakti ◽  
Andi Rahmad Rahim ◽  
Sukaris Sukaris ◽  
...  

This study aims to increase courage children to speaking English using implementation of game in Dahanrejo village, Kebomas, Gresik. The author as tutors and teachers for children in Dahanrejo village in this research. The problems refer to in Dahanrejo village, many children are afraid to speak English because there are no motivation to increase knowledge related studying English, therefore they have lack vocabulary and also teachers can’t explore the other media to teaching English in the classroom. The results of the action research given by the authors are the application of the media can improve the English language skills especially speaking of students and be able to provide variations in indoor learning that are not only fixed on the Package Books and Students Worksheets (LKS). Not only that, but the authors also found that the media was able to change the mood of students who did teaching through the media and were more interested in English.


Author(s):  
Mohamed el Mouden

In this work, the Dutch critical thinker and the socio-cognitive researcher on discourse and power issues, Teun A. Van Dijk, gives us a critical analysis of power and its discursive strategies for domination. It exposes us some forms of abuse of speech on the part of power (such as the media) that end up provoking hate discrimination and rejection of the other.


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