scholarly journals Phytochemical Analysis, Antibacterial Activity, FTIR and GCMS Analysis of Ceropegia juncea Roxb.

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Visveshwari M. ◽  
Subbaiyan B. ◽  
Thangapandian V.
Keyword(s):  
Antibacterial Activity ◽  
Functional Group ◽  
Methanol Extract ◽  
Biological Activities ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Major Peak ◽  
Zone Of Inhibition ◽  
Natural Health ◽  
Aerial Parts

The present study was focused on the preliminary phytochemical, antibacterial activity, FTIR and GC-MS analysis of aerial parts of C. juncea. Phytochemical analysis of three extracts showed that the presence of alkaloids, tannins and flavonoids. The methanol extract of aerial parts were found to be exhibit highest zone of inhibition against S. aureus (19.3mm), P. florescence (17.6mm) and K. pneumonia (17.0mm). FTIR analysis of the methanol extract given the major peak observed was at wavenumber 3354.08cm-1 that indicates the presence of O-H Alcohol functional group. 29 components were identified through the GCMS analysis of methanol extract. From results to confirm the fact that C. juncea possesses potential of bioactive compounds which are responsible for the biological activities that is useful for natural health.

scholarly journals Evaluation of cytotoxicity, anti-herpes simplex virus type 1 (HSV-1) and antibacterial activities of Ficus vasta and phytoconstituents

2013 ◽  
Vol 3 (1) ◽  
pp. 211-218 ◽  
Author(s):  
Khaled Rashed ◽  
Lucy Ono
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Vero Cells ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Potential Candidate ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Hsv 1

This study deals with the evaluation of in vitro cytotoxicity of the methanol 80% extract of Ficus vasta aerial parts in Vero cells, anti-HSV-1 and antibacterial activities and the determination of the phytochemical content of the F. vasta methanol extract. The extract was tested for its cytotoxicity in Vero cells and for anti-HSV-1 and against bacterial strains as S. aureus, S. epidermidis, E. coli and P. aeruginosa and also phytochemical constituents were detected. The results showed that this extract presented a CC50 value of 389 µg/mL, an intermediate value taking into account other researchers reported for some Moraceae species. Regarding the other biological activities, antiviral effect was not demonstrated by a HSV-1 challenge dilution of 100x tissue culture infective dose 50% (TCID50) and also by the disk-diffusion method at the higher non-toxic concentration of the extract (119 µg/mL). Phytochemical analysis of the methanol extract proved the presence of carbohydrates, tannins, flavonoids, coumarins, alkaloids and triterpenes. Chromatographic separation of the methanol extract resulted in the isolation and identification of ?-sitosterol, stigmasterol, lupeol, ursolic acid, naringenin, luteolin, quercetin, vitexin, quercetin 3-O-?-glucoside and rutin. The results indicated that F. vasta aerial parts methanol extract is potential candidate for experiments of biological activity screening that are not anti-HSV-1 or antibacterial against the strains evaluated in this study, to be carried on in the future.DOI: http://dx.doi.org/10.3329/icpj.v3i1.17295 International Current Pharmaceutical Journal, December 2013, 3(1): 211-218

scholarly journals Phytochemical screening, metal concentration determination, antioxidant activity, and antibacterial evaluation of Drymaria diandra plant

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Ashish Phuyal ◽  
Pawan Kumar Ojha ◽  
Biswash Guragain ◽  
Narendra Kumar Chaudhary
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Metal Concentration ◽  
Toxic Metals ◽  
Methanol Extract ◽  
Water Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Concentration Determination ◽  
Medicinal Properties

Abstract Background Several phytochemical constitutes possess natural antioxidant activity and are in fact advantageous in reducing many oxidative stress related diseases. Drymaria diandra of Caryophyllaceae family is one such plant which exhibits various medicinal properties. The aim of the current study is to provide detailed exploration of phytochemical constitutes, metal concentration determination, antioxidant, and antibacterial activity of Drymaria diandra. Results Qualitative phytochemical analysis showed the presence of maximum number of metabolites in methanol-water (1:1) extract. The heavy and toxic metals such as As, Cd, Cr, and Pb were almost near to the detection limit. The concentration of Fe (19.64 mg/l) was highest followed by Mn (2.35 mg/l). The (IC50) value for methanol extract was found 195.61 which is greater than the standard Ascorbic acid. Antibacterial activity of methanol extract was found higher for Staphylococcus aureus and Escherichia coli with 22-mm and 14-mm sized diameter of zone of inhibition respectively and methanol-water extract for Proteus vulgaris with 17-mm diameter of zone of inhibition. Conclusion The findings of the present study showed the presence of various valuable phytochemical constitutes responsible to give antibacterial and antioxidant potency. The presence of bio-metals and the absence of toxic metals further highlight the importance of plant as the source of food that bears medicinal properties.

Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis

2021 ◽  
Vol 2 (1) ◽  
pp. 23-33
Author(s):  
Abdul Aziz ◽  
Veggy Nadya Yuliawan ◽  
Paula Mariana Kustiawan
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Polar Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Content ◽  
Color Changes

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2  mm ± 1.5) at  200µg/mL concentration.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF SWIETENIA MAHAGONI – A HIGHLY MEDICINAL PLANT IN INDIA

2016 ◽  
Vol 9 (6) ◽  
pp. 62
Author(s):  
Dhanapaul Vinoth Kumar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Leaf Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Volatile Oils ◽  
Medicinal Uses ◽  
Antibacterial Study ◽  
Active Constituents ◽  
Swietenia Mahagoni

The present study was designed to evaluate the phytochemical analysis and antibacterial activity of Swietenia mahagoni leaf extract. The leaf extract was subjected to a variety of phytochemical analysis. The qualitative phytochemical tests exhibited the presence of common phytocompounds like alkaloids, tannins, saponins, phlobatannins, flavanoids, terpenoids and volatile oils as major active constituents. The plant (Swietenia mahagoni) leaf has valuable medicinal uses, hence petroleum ether (18.2g) was found to be a good solvent for the phytochemical and antibacterial study. Similarly, the Methanolic leaf extract of Swietenia mahagoni has highest zone of inhibition on Bacillus subtilis.

scholarly journals Phytochemical Screening, Antimicrobial and Antioxidant Activities of Sonchus Oleraceus l (Leaves) Extracts

2020 ◽  
pp. 1-4
Author(s):  
Hatim MY Hamadnalla ◽  
◽  
Mahdi Abd Elmageed Mohammed ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Sonchus Oleraceus

The objective of this study to examine phytochemical analysis of sonchus oleraceus l, (asteraceae) (moliata) extracts which were extracted by four solvents, petroleum ether, chloroform, methanol and distill water and to assess their potential antibacterial against four standard bacteria gram-positive and gramnegative (bacillus subtilis staphylococcus aureu, salmonella typhi, and pscudomona aeruginosa) and antioxidant activities. (moliata) extracts using cold maceration method to extract sonchus oleraceus l, (asteraceae) locally known as (moliata) extracts, where dpph assay and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. The results showed that tannins, triterpenes, a sterols, flavonoids and cardiac glycosides compounds were present in the sonchus oleraceus l, (asteraceae) lives (moliata) extracts. The methanol extract of leaves showed high antioxidant potential (77± 0.03 % ) and chloroform showed low antioxidant potential activity (4±0.09) compare with propyl gallate as standard (93± 0.010 %) against dpph. Chloroform extract was effective and give moderate zone of inhibition against staphylococcus aureus. Methanol extract was effective and showed moderate zone of inhibition against staphylococcus aureus, bacillus subtilis, salmonella typhi and pseudomonas aeruginosa.

scholarly journals Study on antibacterial activity of Calotropis procera

2014 ◽  
Author(s):  
Muhammad Muzammal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Calotropis Procera ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Crude Extracts ◽  
Mic Value

Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i.e. Salmonella typhi and E.coli. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of Calotropis procera was carried out in disc method and also determined MIC value at 600nm through optical density using spectrophotometer. The zone of inhibition produced by extracts was examined and compares it with zone produced by antibiotics. The effect exhibited by ethanolic extract of leaves and flower was significantly greater than the aqueous and methanolic extract of leaves and flower. Crude extracts i.e. latex, leaves, fruit and flower crude extracts. Among them, flower crude extracts shows similar zone of inhibition to test anitibiotics.while in MIC value, we made different concentration of extracts and antibiotics.i.e for crude we made 25%, 50%.75% and 100% concentration of crude juice and for the aqueous, methanolic and ethanolic we made 0.5mg/mL,1mg/mL,2mg/mL,3mg/mL,4mg/mL, 5mg/mL and 6mg/mL in Dimethyl salfoxide, and same antibiotics concentration. Aqueous leaves extracts show MIC at 0.5mg/ml against E.coli while against Salmonella it shows MIC at 1mg/ml... We also determine phytochemical analysis for presences of different compounds in crude extracts. The obtained results provide a support for the use of Calotropis procera, in traditional medicine and suggest its further advance investigation.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

scholarly journals PHYTOCHEMICAL INVESTIGATION, GC-MS PROFILE AND ANTIMICROBIAL ACTIVITY OF A MEDICINAL PLANT RUTA GRAVEOLENS L. FROM ETHIOPIA

2017 ◽  
Vol 9 (6) ◽  
pp. 29 ◽  
Author(s):  
Henok Gulilat Azalework ◽  
Sahabjada . ◽  
Asif Jafri ◽  
Md Arshad ◽  
Tabarak Malik
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Micrococcus Luteus ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Phytochemical Analysis ◽  
Ruta Graveolens ◽  
Zone Of Inhibition ◽  
Ms Analysis

Objective: This study was designed to screen the phytochemicals present in various solvents extracts of Ruta graveolens (Rue) and furthermore to investigate their antimicrobial activity.Methods: The leaves, stems and seeds of Rue were extracted using four different solvents viz. ethanolic, methanolic, chloroform, and aqueous of varying polarity. The phytochemical screening was carried out qualitatively and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis was performed to identify major phytoconstituents present in the methanolic leaf extract. The antimicrobial effect of extracts was evaluated against six microbial strains namely Bacillus subtillis, Escherichia coli, Proteus vulgaris, Candida albicans, Candida tropicalis and Micrococcus luteus with disc diffusion method.Results: Phytochemical analysis revealed the presence of various secondary metabolites such as flavonoids, alkaloids, terpenoids, saponins and carotenoid. The methanolic leaf extract showed the presence of both tannin and phenolic contents in the higher amount, whereas aqueous extract displayed in the least amount. GC-MS analysis of methanolic leaf extract revealed the presence of approximately 26 phytochemical constituents. The antimicrobial assay revealed that B. subtilis showed a high zone of inhibition (20 mm) at 200 mg/ml of methanolic extract. However, E. coli and C. tropicalis did not show any zone of inhibition against each solvent extract.Conclusion: In conclusion, secondary metabolites present in the extracts have biological activities which warrant further to evaluate in vivo pharmacological studies.

scholarly journals Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3625
Author(s):  
Christos Ganos ◽  
Nektarios Aligiannis ◽  
Ioanna Chinou ◽  
Nikolaos Naziris ◽  
Maria Chountoulesi ◽  
...  
Keyword(s):  
Free Radical ◽  
Biological Activities ◽  
Biological Membrane ◽  
3D Structure ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Endemic Plant ◽  
Aerial Parts ◽  
Potential Applications ◽  
First Time

Rindera graeca is a Greek endemic plant of the Boraginaceae family which has never been studied before. Consequently, this study attempted to phytochemically examine the aerial parts of this species. Nine phenolic secondary metabolites were identified, consisting of seven caffeic acid derivatives and two flavonol glucosides, namely rutin and quercetin-3-rutinoside-7-rhamnoside. These flavonoids, together with rosmarinic acid, were isolated via column chromatography and structurally determined through spectral analysis. Quercetin-3-rutinoside-7-rhamnoside is an unusual triglycoside, which is identified for the first time in Rindera genus and among Boraginaceae plants. This metabolite was further examined with thermal analysis and its 3D structure was simulated, revealing some intriguing information on its interaction with biological membrane models, which might have potential applications in microcirculation-related conditions. R. graeca was also analyzed for its pyrrolizidine alkaloids content, and it was found to contain echinatine together with echinatine N-oxide and rinderine N-oxide. Additionally, the total phenolic and flavonoid contents of R. graeca methanol extract were determined, along with free radical inhibition assays. High total phenolic content and almost complete inhibition at experimental doses at the free radical assays indicate a potent antioxidant profile for this plant. Overall, through phytochemical analysis and biological activity assays, insight was gained on an endemic Greek species of the little-studied Rindera genus, while its potential for further applications has been assessed.

Synthesis, Characterization, and Antibacterial Evaluation of Curcumin-Sulfanilamide Compound

2020 ◽  
Vol 840 ◽  
pp. 265-269
Author(s):  
Nurjanah Nurjanah ◽  
Endang Saepudin
Keyword(s):  
Antibacterial Activity ◽  
Functional Group ◽  
Biological Activities ◽  
Curcuma Longa ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Ftir Spectrophotometry

Curcumin, a diarylheptanoids compound which isolated primary from Curcuma longa, exhibits a variety of exciting biological activities, including as an antibacterial agent. In the present study, a sulfanilamide-contained curcumin compound was synthesized and characterized to investigate the antibacterial activity against gram-positive bacteria S. aureus, B. subtilis and gram-negative bacteria E. coli. The characterization of the synthesized compound was determined by analysing peak absorbance, functional group, and molecular weight using mass spectroscopy, UV/Vis and FTIR spectrophotometry. Curcumin-sulfanilamide compound exhibited the best antibacterial activity against gram-negative bacteria compared to curcumin and the curcumin-derived compound containing isoxazole with inhibitory zone of 11 mm.

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