In vitro test on the ability of a yeast cell wall based product to inhibit the Escherichia coli F4ac adhesion on the brush border of porcine intestinal villi1

2012 ◽  
Vol 90 (suppl_4) ◽  
pp. 275-277 ◽  
Author(s):  
P. Trevisi ◽  
D. Priori ◽  
G. Gandolfi ◽  
M. Colombo ◽  
F. Coloretti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Yeast Cell ◽  
Brush Border ◽  
Yeast Cell Wall ◽  
In Vitro Test ◽  
Vitro Test

Antioxidative and antimutagenic activity of yeast cell wall mannans in vitro

2001 ◽  
Vol 497 (1-2) ◽  
pp. 213-222 ◽  
Author(s):  
Lı́via Križková ◽  
Zdena Ďuračková ◽  
Jozef Šandula ◽  
Vlasta Sasinková ◽  
Juraj Krajčovič
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Yeast Cell Wall ◽  
Antimutagenic Activity

scholarly journals In vitro efficacy of several disinfectants against Salmonella enterica serovar Enteritidis and Escherichia coli strains from poultry

2016 ◽  
Vol 46 (8) ◽  
pp. 1438-1442
Author(s):  
Sonia Martínez-Martínez ◽  
Sheila Yubero-Delgado ◽  
Elías-Fernando Rodríguez-Ferri ◽  
Rafael Frandoloso ◽  
Álvaro Álvarez-Estrada ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Enterica ◽  
Environmental Control ◽  
Control Measures ◽  
In Vitro Test ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Vitro Test ◽  
The Individual ◽  
Ammonium Compounds

ABSTRACT The efficacy of 28 individual or blended disinfectants against avian Salmonella enterica serovar Enteritidis and Escherichia coli strains was determined. An in vitro test in the presence and absence of serum as source of organic material was conducted. Povidone-iodine (releasing 1% available iodine), 1% potassium permanganate, 70% ethanol, 2% chlorhexidine digluconate and three commercial formulations based on quaternary ammonium compounds + formaldehyde or cresol derivates were the most effective against all strains tested and reduced bacterial counts by more than 106 times (6-log10) regardless of the presence of organic matter. These commercial compounds as well as ethanol and chlorhexidine among the individual substances tested might be helpful in the adoption of environmental control measures against these two enterobacteria in poultry industry.

scholarly journals Comprehensive Evaluation of the Efficiency of Yeast Cell Wall Extract to Adsorb Ochratoxin A and Mitigate Accumulation of the Toxin in Broiler Chickens

Toxins ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 37 ◽  
Author(s):  
Suvi Vartiainen ◽  
Alexandros Yiannikouris ◽  
Juha Apajalahti ◽  
Colm A. Moran
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Ochratoxin A ◽  
Broiler Chickens ◽  
Animal Feed ◽  
Animal Health ◽  
Yeast Cell Wall ◽  
In Vivo Model

Ochratoxin A (OTA) is a common mycotoxin contaminant in animal feed. When absorbed from the gastrointestinal tract, OTA has a propensity for pathological effects on animal health and deposition in animal tissues. In this study, the potential of yeast cell wall extracts (YCWE) to adsorb OTA was evaluated using an in vitro method in which consecutive animal digestion events were simulated. Low pH markedly increased OTA binding to YCWE, which was reversed with a pH increased to 6.5. Overall, in vitro analysis revealed that 30% of OTA was adsorbed to YCWE. Additional computational molecular modelling revealed that change in pH alters the OTA charge and modulates the interaction with the YCWE β-d-glucans. The effectiveness of YCWE was tested in a 14-day broiler chicken trial. Birds were subjected to five dietary treatments; with and without OTA, and OTA combined with YCWE at three dosages. At the end of the trial, liver OTA deposition was evaluated. Data showed a decrease of up to 30% in OTA deposits in the liver of broilers fed both OTA and YCWE. In the case of OTA, a tight correlation between the mitigation efficacy of YCWE between in vitro and in vivo model could be observed.

scholarly journals EFEKTIVITAS EKSTRAK BUAH SAWO, BAWANG PUTIH, DAUN ANDONG, DAN BUAH PARE TERHADAP PERTUMBUHAN BAKTERI ESCHERICHIA COLI

2018 ◽  
Vol 7 (2) ◽  
pp. 144-149
Author(s):  
Susiwati Susiwati
Keyword(s):  
Escherichia Coli ◽  
Inhibition Zone ◽  
Garlic Extract ◽  
In Vitro Test ◽  
E Coli ◽  
Significant Difference ◽  
Vitro Test ◽  
Inhibition Zones ◽  
Escherichia Coli Bacteria

This research aims to determine the inhibition of sapodilla fruit, garlic, andong leaves and pare fruit toward the growth of escherichia coli bacteria. Antimicroba test used paper disc diffusion was in-vitro test. Sapodilla fruit, garlic, andong leaves and pare fruit were extracted by using maceration process. The extracts were tested on the growth of E- coli bacteria.  The highest inhibition zone (6,7mm) was found in andong leaves extract. The highest inhibition zone was 8.3 mm, whereas the inhibition of pare fruit did not not provide the inhibitory zone. It can be concluded that garlic extract, sapodolla extract and decoction of andong leaves have highly inhibitory in vitro. Based on stastistical analysis, there was Significant difference betwen the effectiveness of garlic extract with a decoction of andong leaves but the effectivess of garlic extract with sapodilla extract was not meaningful. Whereas pare fruit did not give any inhibition zones. From the result of this research, the society can be encouraged to consume andong leaves or sapodilla fruit to treat diarrhea. In addition, garlic spices and pare fruit also can be used to overcome diarrhea, which is caused by the bacterium E. coli.

scholarly journals In Vitro Binding Potentials of Bentonite, Yeast Cell Wall and Lactic Acid Bacteria for Aflatoxin B1 and Ochratoxin A

2018 ◽  
Vol 12 (2) ◽  
pp. 7-13 ◽  
Author(s):  
Damoon Ghofrani Tabari ◽  
Hassan Kermanshahi ◽  
Abolghasem Golian ◽  
Reza Majidzadeh Heravi ◽  
◽  
...  
Keyword(s):  
Cell Wall ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Yeast Cell ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Yeast Cell Wall ◽  
In Vitro Binding ◽  
Vitro Binding

In vitro zearalenone adsorption by a mixture of organic and inorganic adsorbents: application of the Box Behnken approach

2015 ◽  
Vol 8 (3) ◽  
pp. 291-299 ◽  
Author(s):  
J.G. Bordini ◽  
D. Borsato ◽  
A.S. Oliveira ◽  
M.A. Ono ◽  
T.H. Zaninelli ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Activated Charcoal ◽  
High Efficiency ◽  
Statistical Design ◽  
Yeast Cell Wall ◽  
Quadratic Model ◽  
Wide Range

Zearalenone (ZEA) adsorption by a mixture of organic (yeast cell wall) and inorganic (activated charcoal) adsorbents was evaluated by an incomplete Box Behnken (33) statistical design with a quintuplicate at the central point. The variables analysed were different ratios of adsorbents (yeast cell wall and activated charcoal) at 100:0, 87.5:12.5 and 75:25, pH (3.0, 4.5 and 6.0) and ZEA concentrations (300, 750 and 1,200 ng/ml). The adsorbent mixture at 75:25 showed higher efficiency for ZEA adsorption (≯96.1%) than the 87.5:12.5 ratio (81.3 to 93.7%) and with the pure yeast cell wall (78.1 to 55.7%). The significant variables were the ratio of adsorbent mixture and ZEA concentration. The effect of pH was not significant (P=0.05), indicating that the binding between ZEA and the adsorbent would be stable at different pH (3.0, 4.5 and 6.0). The quadratic model obtained by the Box Behnken (33) design can be used for predictive purposes, because it showed a non-significant deviation (P=49.54%) and a good correlation coefficient (R2=0.98), suggesting that the ZEA adsorption would be maximum (100%) when the adsorbent mixture is set at 75:25 and the ZEA concentration at 300 ng/ml. Although the predictive model showed that an increase in adsorption efficiency could occur in a smaller ZEA concentration (300 ng/ml), the mixture at the 75:25 ratio presented high efficiency (≯98%) in adsorption when high ZEA concentrations were used (1,200 ng/ml), indicating that these mixtures would be able to adsorb a wide range of ZEA concentrations. Therefore, this mixture of yeast cell wall and activated charcoal adsorbents at 75:25 might be a candidate for further in vivo testing.

Efficacy of b-glucans and manna oligosaccharides (Yeast Cell Wall) and hydrated sodium calcium aluminosilicate (HSCAS) in preventing aflatoxicosis in bovine calves

2017 ◽  
Author(s):  
Omer Naseer ◽  
Jawaria Khan ◽  
Muhammad Khan ◽  
Muhammad Omer ◽  
Muhammad Avais ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Hematological Parameters ◽  
Negative Control ◽  
Yeast Cell Wall ◽  
Feed Consumption ◽  
Sodium Calcium ◽  
Calcium Aluminosilicate

The objective of this study was to determine the response of bovine calves against aflatoxin B1 (AFB1) in terms of feed consumption, hematological and serum biochemical parameters and to compare the efficacy of two different mycotoxin adsorbents, in vitro and in vivo. 36 bovine calves were divided into 4 groups. Group A was fed AFB1 added feed with the addition of â-glucans and Mannan oligosaccharides (Yeast Cell Wall), group B was fed AFB1 with hydrated sodium calcium aluminosilicate (HSCAS) and group C was fed AFB1 contaminated feed without addition of mycotoxin binders while group D was kept as negative control. AFB1 was given by gelatinized capsules at a dose rate of 1.0mg/ kg/ animal/ day. Results revealed average daily feed intake (ADFI) of AFB1 treated bovine calves significantly reduced (P less than 0.05) and all hematological parameters i.e; TEC, HGB, TLC, lymphocytes, neutrophils and monocytes, MCHC, HCT and MCH decreased significantly (P less than 0.05). Moreover, serum levels of AST, ALT, Creatinine and BUN were significantly increased (P less than 0.05) in response to AFB1. When compared between groups, YCW significantly (P less than 0.05) improved the feed consumption of bovine calves while HSCAS significantly reduced (P less than 0.05) the AFB1 induced deleterious alterations in hematology and serum biochemistry.

A novel cancer vaccine platform utilizing autologous tumor lysate loaded into capped yeast cell wall particles.

2017 ◽  
Vol 35 (7_suppl) ◽  
pp. 169-169
Author(s):  
Kaitlin M. Peace ◽  
Timothy J Vreeland ◽  
Diane F Hale ◽  
Doreen O Jackson ◽  
Julia M Greene ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Ex Vivo ◽  
Yeast Cell Wall ◽  
Autologous Tumor ◽  
Tumor Lysate ◽  
Vaccine Platform ◽  
Gm Csf ◽  
Murine Melanoma

169 Background: Our current tumor lysate, particle-loaded, dendritic cell (TLPLDC) vaccine employs yeast cell wall particles (YCWP) to deliver antigen to DC ex vivo and is being tested in a randomized phase IIb trial. This strategy may be improved by injecting TL-loaded YCWP as an intradermal vaccine, with in vivo DC uptake. Silicate capping should allow YCWP to retain TL longer and stimulate DC uptake. Here, we present preclinical data on the tumor lysate, particle only (TLPO) vaccine concept vs. TLPLDC. Methods: To test the TLPO concept, YCWP were loaded with fluorescence (flr) labeled albumin and capped (cp) vs. uncapped (ucp) particles were compared for flr leak. Next, YCWP were added to cultured macrophages to evaluate DC uptake. Cells were then lysed, centrifuged, and flr in the cytoplasm vs. organelles measured. 3 C57B mice were then injected with 100ml NaCl, 106empty cp YCWP, or 1mcg GM-CSF. 5 hours (hr) post-injection, 100ml NaCl was injected into the same site, withdrawn, and examined via microscope to count monocytes. TLPO was compared to TLPLDC in a B16 murine melanoma survival model. Finally, 4 grey horses with equine melanoma were treated with autologous TLPO, injected biweekly for 4 vaccinations. Target lesions were assessed over 6 months. Results: Compared to ucp YCWP, cp had decreased flr leak at 1 (15.8% vs. 24.7%) and 2 hr (6.7% vs. 16.6%), increased uptake by DC (2hr flr readings 11065 vs. 3928) and higher delivery to DC cytoplasm (68.9% vs. 48.8%). Empty cp YCWP showed increased recruitment of monocytes (276/hpf) vs. GM-CSF (55/hpf) or NaCl alone (18/hpf). In the B16 murine melanoma model, the median survival time in days was 21 for controls (n=5), 42 for TLPLDC (n=10), and 56 for TLPO (n=20). At 6 months, the equine model revealed 1 complete and 3 partial responses (50%, 68%, and 45% tumor reduction). Conclusions: Silicate capping of the YCWP effectively limits leakage of contents, improves uptake by DC and delivery to the cytoplasm without the need for GM-CSF. In early animal studies, TLPO appears to have equivalent efficacy to TLPLDC, but eliminates the need for in vitro DC loading. We plan to perform a bridging study comparing TLPO to TLPLDC after enrollment is complete in our phase IIb TLPLDC melanoma trial.

Effect of temperature on in vitro ochratoxin A biosorption onto yeast cell wall derivatives

2005 ◽  
Vol 40 (9) ◽  
pp. 3008-3016 ◽  
Author(s):  
Diana Ringot ◽  
Benoit Lerzy ◽  
Jean Paul Bonhoure ◽  
Eric Auclair ◽  
Eric Oriol ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Ochratoxin A ◽  
Yeast Cell Wall ◽  
Effect Of Temperature
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