scholarly journals LCMS Measurement of Steroid Biomarkers Collected from Palmar Sweat.

2020 ◽  
Author(s):  
Jacob Hyde ◽  
J. Ray Runyon
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Real World ◽  
Liquid Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Non Invasive ◽  
Stress Task ◽  
Monitoring Study ◽  
Study Participants ◽  
Sweat Collection

<div>Human eccrine sweat contains numerous biomarkers which can provide information on health,</div><div>performance, and aging. Non-invasive collection and measurement of biomarkers has become</div><div>especially important in recent times given viral outbreaks like SARS-CoV-2. In the current study</div><div>we describe a method of sweat collection from palmar surfaces in participants via surface capture</div><div>using glass beads and the resulting analysis of biomarkers from very low volumes of sweat using</div><div>liquid chromatography mass spectrometry with selected ion monitoring. Study participants</div><div>underwent a cognitive and physical stress task with easy and hard conditions with sweat being</div><div>collected after each task. Resulting analysis found a signal for 22 steroid biomarkers and we</div><div>report detailed information on selected biomarkers, given their applicability to timely real-world</div><div>exemplars, including cortisol, dehydroepiandrosterone, allopregnanolone, estrone, aldosterone,</div><div>and 20a/b-dihydrocortisone.</div><div><br></div>

scholarly journals LCMS Measurement of Steroid Biomarkers Collected from Palmar Sweat.

2020 ◽  
Author(s):  
Jacob Hyde ◽  
J. Ray Runyon
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Real World ◽  
Liquid Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Non Invasive ◽  
Stress Task ◽  
Monitoring Study ◽  
Study Participants ◽  
Sweat Collection

<div>Human eccrine sweat contains numerous biomarkers which can provide information on health,</div><div>performance, and aging. Non-invasive collection and measurement of biomarkers has become</div><div>especially important in recent times given viral outbreaks like SARS-CoV-2. In the current study</div><div>we describe a method of sweat collection from palmar surfaces in participants via surface capture</div><div>using glass beads and the resulting analysis of biomarkers from very low volumes of sweat using</div><div>liquid chromatography mass spectrometry with selected ion monitoring. Study participants</div><div>underwent a cognitive and physical stress task with easy and hard conditions with sweat being</div><div>collected after each task. Resulting analysis found a signal for 22 steroid biomarkers and we</div><div>report detailed information on selected biomarkers, given their applicability to timely real-world</div><div>exemplars, including cortisol, dehydroepiandrosterone, allopregnanolone, estrone, aldosterone,</div><div>and 20a/b-dihydrocortisone.</div><div><br></div>

Confirmatory Identification of Carbadox-Related Residues in Swine Liver by Gas-Liquid Chromatography/Mass Spectrometry with Selected Ion Monitoring

1982 ◽  
Vol 65 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Martin J Lynch ◽  
S Richard Bartolucci
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Carboxylic Acid ◽  
Gas Liquid Chromatography ◽  
Target Tissue ◽  
Liquid Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Methyl Ester Derivative ◽  
Chromatography Mass Spectrometry ◽  
Swine Liver

Abstract A confirmatory method has been developed to identify quinoxaline-2-carboxylic acid, the carbadox tissue residue, in swine liver, a target tissue, at the regulatory level of 30 μg/kg. Quinoxaline-2-carboxylic acid (QCA) is isolated from liver hydrolysates by solvent extraction and ion-exclusion chromatography, and a methyl ester derivative (CP-25,536 or QME) is identified by gas-liquid chromatography/ mass spectrometry with selected ion monitoring. The relative intensities of 3 ions: the base peak at m/z = 130, a second significant mass at m/z = 158, and the molecular ion (M+) at m/z = 188, are monitored simultaneously with a quadrupole mass spectrometer. Validation studies consisting of the analysis of liver fortified with QCA at the regulatory level and analysis of swine specimens containing physiologically incurred carbadox residues demonstrated that peak height ratios of ions in these tissue extracts corresponded to ion intensities of standards monitored at m/z = 188,158, and 130.

scholarly journals Identification of Fumonisin B2, HT-2 Toxin, Patulin, and Zearalenone in Dried Figs by Liquid Chromatography–Time-of-Flight Mass Spectrometry and Liquid Chromatography–Mass Spectrometry

2008 ◽  
Vol 71 (7) ◽  
pp. 1500-1504 ◽  
Author(s):  
H. Z. ŞENYUVA ◽  
J. GILBERT
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Uv Light ◽  
Time Of Flight ◽  
Fungal Metabolites ◽  
Liquid Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Flight Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Fumonisin B2

Dried figs from Turkey that were visibly moldy (or fluorescent under UV light) and thus rejected as unsuitable for human food were screened for the presence of fungal metabolites. Crude solvent extracts from individual figs were directly analyzed by liquid chromatography combined with time-of-flight mass spectrometry to generate accurate mass data for all detectable components. A comparison of these data with a metabolite database indicated the presence of fumonisins B2 and B4, patulin, HT-2 toxin, and zearalenone among various other metabolites. Portions of the same figs were reextracted and then analyzed by conventional liquid chromatography–mass spectrometry. On the basis of coincident retention times and by matching selected ion monitoring for coincident ions with that of authentic standards, the identification of fumonisin B2, HT-2 toxin, patulin, and zearalenone was confirmed.

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