scholarly journals Territorial distribution alleles of SSR-loci of chloroplast DNA of scots pine (Pinus sylvestris L.) in Belarus

2021 ◽  
Vol 65 (1) ◽  
pp. 87-95
Author(s):  
V. E. Padutov ◽  
D. I. Каgаn ◽  
S. I. Ivanovskaya ◽  
O. Yu. Baranov ◽  
O. A. Razumova
Keyword(s):  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Chloroplast Dna ◽  
Molecular Genetic Analysis ◽  
Point Of View ◽  
Limited Area ◽  
Frequency Of Occurrence ◽  
Scotch Pine ◽  
Allelic Variants ◽  
Ssr Loci

Scotch pine (Pinus sylvestris L.) is one of the main forest forming species in Belarus, which cover 49.2 % of the country’s forested area. The aim of the study was to investigate spatial distribution of microsatellite (SSR) alleles (chloroplast DNA) and identify the features of the genetic structure and genogeographic differentiation of P. sylvestris populations in Belarus. Molecular genetic analysis of six SSR loci of Scotch pine cpDNA in samples form 73 naturally originated stands was carried out. 35 allelic variants of loci PCP1289, PCP4507, PCP83314, PCP71987, PCP26106, PCP30277 were identified. The analysis of the geographic distribution of the dominant allelic variants showed that the population structure of the pine forest is rather homogeneous. Certain regional differences in a number of cases were found for less common, but also widespread variants. One group of alleles is characterized by an increase or decrease in the frequency of occurrence in the direction from the southwest to the northeast. Another group includes allelic variants which share is maximal in the zone covering the Grodno region, the southwestern part of the Minsk region and the Gomel region, while to southwest and to northeast from this zone their frequency of occurrence decreases or is absent. A number of alleles were found only in one of the analyzed stands or in a limited area, which may indicate their local origin as a result of spontaneous mutations. The obtained results are important both from a general biological point of view in studying the evolution and formation of the genetic structure of P. sylvestris in Belarus, and from a practical point of view, since they allow to improve the forest seed zoning of the species.

scholarly journals Population genetic structure of pedunculate oak (Quercus robur L.) in Belarus according to the analysis of chloroplast DNA

2021 ◽  
pp. 59-70
Author(s):  
Vladimir E. Padutov
Keyword(s):  
Genetic Structure ◽  
Chloroplast Dna ◽  
Population Genetic Structure ◽  
Population Genetic ◽  
Quercus Robur ◽  
Phylogenetic Trees ◽  
Rflp Analysis ◽  
Pedunculate Oak ◽  
Maximum Likelihood Methods ◽  
The Republic

Pedunculate oak (Quercus robur L.) is one of the main forest forming species in the Republic of Belarus. Its population genetic structure was formed under the influence of various migration processes. Six chloroplast DNA loci (µdt1, µdt3, µdt4, µcd4, µcd5 and µkk4) were used for the genogeographic study. The material for the analysis was collected in 100 oak forest stands (2325 samples); 18 allelic variants were identified, which are grouped into 17 different combinations (haplotypes). Five of them are widespread (the proportion of occurrence varies from 7 to 48 %, totalling 85 %). The remaining 12 are rare (the proportion of occurrence varies from 1 to 3 %, totalling 15 %). Phylogenetic trees constructed using the nearest neighbour and maximum likelihood methods show the presence of two groups (branches) of haplotypes. One of it comprises 8 variants including 2 dominant haplotypes and the other comprises 9 variants including 3 dominant haplotypes. PCR-RFLP analysis of chloroplast DNA showed that the pedunculate oak in Belarus originates from the Balkan refugium. Haplotype No. 1 (µdt189, µdt3123, µdt4142, µcd494, µcd574, µkk4109) is found almost everywhere in Belarus with the exception of the southwest and northeast, while haplotype No. 8 (µdt189, µdt3121, µdt4142, µcd494, µcd574, µkk4109) is mainly localised in the southwest and northeast. Haplotypes No. 3 (µdt189, µdt3120, µdt4141, µcd494, µcd575, µkk4109) and No. 7 (µdt189, µdt3122, µdt4142, µcd494, µcd574, µkk4109) predominantly found in the west of the country. Haplotype No. 2 (µdt190, µdt3120, µdt4141, µcd495, µcd574, µkk4109) is typical for the southeast.

scholarly journals Growth stimulants application for pre-sowing treatment of Scotch pine (Pinus Sylvestris) seeds in the ribbon forest in Priirtyshje

2019 ◽  
Vol 23 (136) ◽  
pp. 13-19
Author(s):  
S.A. Kabanova ◽  
◽  
I.S. Kochegarov ◽  
M.A. Danchenko ◽  
◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Scotch Pine

POLYMORPHISM OF PROLACTIN AND SOMATOTROPIN GENES IN DAIRY CATTLE OF THE REPUBLIC OF TATARSTAN

2021 ◽  
pp. 22-25
Author(s):  
Р.Р. ШАЙДУЛЛИН ◽  
А.Б. МОСКВИЧЕВА ◽  
Т.Х. ФАИЗОВ ◽  
Л.Р. ЗАГИДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Dna Markers ◽  
Frequency Of Occurrence ◽  
Allelic Variants ◽  
Black And White ◽  
Prolactin Gene ◽  
White Breed ◽  
White Cattle ◽  
Cattle Herds ◽  
The Republic

Изучен полиморфизм и определена частота встречаемости аллельных вариантов по генам пролактина (PRL) и соматотропина (GH) у коров в условиях двух племенных хозяйств Республики Татарстан. Объектом исследования были животные черно-пестрой породы первого (n=151) и третьего (n=168) отелов и холмогорской породы первого (n=160) и третьего (n=143) отелов. Проведено генотипирование коров по генам PRL и GH методом ДНК-диагностики. Установлено, что среди молочного скота преимущественно встречаются особи с генотипом PRL АА (70,0—77,5%), с частотой аллеля А гена пролактина — 0,83—0,87, аллеля В — 0,13—0,17. Встречаемость аллеля В была выше среди холмогорских коров (0,16—0,17). В стаде полновозрастных животных черно-пестрой породы имеется сдвиг генетического равновесия в сторону генотипов PRL AA и PRL BB (χ2=3,97; Р<0,05). В исследуемых стадах крупного рогатого скота выражено преимущество генотипа GH LL с частотой встречаемости 51,0—79,2%. Встречаемость генотипа GH VV у черно-пестрого скота составила 2,0—2,4%, у холмогорского — 5,6—7,0%. Аллель L гена соматотропина преобладает над аллелем V в обеих породах. При этом чаще встречаемость аллеля L GH среди коров холмогорской породы (0,28—0,29). У холмогорских коров больше представлены редкие и желательные аллели генов PRL и GH. Чтобы повысить встречаемость «предпочтительных» в хозяйственном отношении генотипов и аллелей ДНК-маркеров, необходимо в большей степени использовать быков-производителей с желательными аллелями PRL и GH, а именно В и V соответственно. The polymorphism was studied and the frequency of occurrence of allelic variants for the genes of prolactin (PRL) and somatotropin (GH) was determinedin cows in two bred livestock farms of the Republic of Tatarstan. The object of the study were animals of the black-and-white breed of the first (n=151) and third (n=168) calving and the Kholmogory breed of the first (n=160) and third (n=143) calving. Genotyping of cows for PRL and GH genes was carried out using method of DNA diagnostics. It was revealed that there were mainly individuals with the PRL AA genotype (70,0–77,5%)among dairy cattle, the frequency of the A allele of the prolactin gene was 0,83–0,87, the B allele was 0,13–0,17. The frequency of allele B was higher among Kholmogory cows (0,16–0,17). There was a shift in the genetic balance towards the PRL AA and PRL BB genotypes (χ2=3,97; Р<0,05) in the herd of full-age animals of the black-and-white breed. The advantage of the GH LL genotype was expressed with a frequency of occurrence 51,0–79,2% in the studied cattle herds. The occurence of the GH VV genotype was 2,0–2,4%in black-and-white cattle, 5,6–7,0% was in Kholmogorycattle. The L allele of the somatotropin gene predominated over the V allele in both breeds. Moreover, the occurrence of the L GH allele among cows of the Kholmogory breed (0,28–0,29) was more often. Rare and desirable alleles of the PRL and GH genes are more represented in Kholmogory cows. In order to increase the occurrence of economically “preferred” genotypes and alleles of DNA markers, it is necessary to use more stud bulls with the desired PRL and GH alleles, namely B and V, respectively.

AN INVESTIGATION OF THE COMPOSITION OF ESSENTIAL OIL IN THE WOODY GREENERY OF SCOTCH PINE (PINUS SYLVESTRIS)

2021 ◽  
Vol 70 (3) ◽  
pp. 31-35
Author(s):  
Anna Mikhailovna Sumenkova ◽  
Dmitry Konstantinovich Gulyaev ◽  
Valentina Dmitrievna Belonogova ◽  
Petr Sergeevich Mashchenko
Keyword(s):  
Essential Oil ◽  
Pinus Sylvestris ◽  
Scotch Pine
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